Oxygen uptake rate, critical oxygen concentration
Marchut-Mikołajczyk O., PhD
Author/User
Keywords: oxygen, uptake rate
equipment required: CTN-920S Elsen oxygen sensor coupled with CO-551 Elmetron
microcomputer oxygen interface
execution time: 30 minutes
Description:
The total oxygen uptake by microbial population was determined by dynamic method ( Taguchi and
Humphrey, 1966) through interrupting aeration and monitoring the decrease in oxygen concentration
in the culture medium. The rate of oxygen consumption by growing bacterial cells was monitored using
the CTN-920S Elsen oxygen sensor coupled with CO-551 Elmetron microcomputer oxygen interface. The
maximum solubility of oxygen in culture medium was measured by determining the steady-state oxygen
concentration in the not inoculated flasks. Recording of changes in dissolved oxygen concentration
(dC/dt) during shortintervals of aeration (up to 10 min) resulted in the following
function:dCdt¼rCx,where C is the oxygen concentration in the liquid phase (mgO2l1);t is the time
(min);Cx is the cell concentration in the liquid phase (mgl1);r is the specific cell respiration rate (min1)
Taguchi, H., Humphrey, A.E., 1966. Dynamic measurement of volumetric oxygen transfer coefficient
in fermentation systems. Journal of Fermentation Technology 44, 881–889
Ryc. Oxygen sensor CO-551, Elmetron