FET09 Title: Development and evaluation of novel 3D nano-structured biosensor for diagnostic assay platforms Contact: [email protected] Project: A key limitation of current biosensors is their restricted durability and shelf life due to the loss of biological activity. This has prevented their widespread application in fields such as medical diagnostics for the third world, wearable biosensors and for use during military operations. To address this limitation a new sensor structure has been invented at UWE that comprises a novel 3D sensor surface using a nano-crystal matrix combined with a novel encapsulation technique to protect the biological layer and permit the creation of robust biosensors. This PhD research project will investigate the key factors that contribute to the performance of the nano-structure-based sensor. It will involve comparison of fabrication techniques for creating a 3D matrix, including the use of carbon nanotubes, self-assembled graphene or metal oxide layers. Sensor surfaces will be analysed using several physical analytical techniques and through a model assay measuring cortisol, which has relevance for body worn sensors. Impedance spectroscopy will be used to determine the binding of target to the sensor. Algorithms for analysis of the output signal will be developed and evaluated to provide the optimum method for establishing a dose dependent relationship.