APPENDIX 1. Voucher information for new sequence data used in this study and GenBank accession numbers for all ITS and trnL-F sequences, listed in that order. AllThysanocarpus sequences were generated for the present study. Among other taxa, accessions from which sequences were generated for the present study are marked with an asterisk. Thelypodieae: Thysanocarpus—1. Thysanocarpus conchuliferus,U. S. A.: California: Santa Barbara Co.: Santa Cruz Island, rocky butte E of Lagunita Secas (originally M.R. Benedict, 28 May 1969; grown under lath at Santa Barbara Botanic Garden), R.B. Philbrick B69-130 (DS), GU246121, GU246184. 2. Thysanocarpus conchuliferus,U. S. A.: California: Santa Barbara Co.: Santa Cruz Island, Twin Harbor, R. Hoffman 655 (RSA), GU246122, GU246185. 3. Thysanocarpus conchuliferus,U. S. A.: California: Santa Barbara Co.: Santa Cruz Island, upper west fork of Orizaba Canyon (fork which intersects main ridge line due S of Frye''s Harbor), S. A. Junak 1053 (RSA), GU246123, GU246186. 4. Thysanocarpus curvipes subsp. amplectens,Mexico: Sonora: Mcpio. Tubutama: 4.5 km SE of Tubutama on road to Magdalena, T. R. Van Devender 2003-262 (NMC), GU246124, GU246187. 5. Thysanocarpus curvipes subsp. amplectens,U. S. A.: Arizona: Cochise Co.: South-southwest of Willcox along Stronghold Canyon East in the Dragoon Mountains ca. 1.93 km southeast of Barret Spring, M. D. Windham 2929 (NMC), GU246125, GU246188. 6. Thysanocarpus curvipes subsp.amplectens,U. S. A.: Arizona: Graham: Camp at confluence of Bonita Creek and Gila River, L.A. McGill 1968 (CAS), GU246126, GU246189. 7. Thysanocarpus curvipes subsp. amplectens,U. S. A.: Arizona: Greenlee Co.: East of Clifton near Arizona Highway 78 on slope overlooking Black Jack Canyon ca. 1.29 km northeast of Dunigan Spring, M. D. Windham 2921 (NMC), GU246127, GU246190. 8.Thysanocarpus curvipes,intermediate between subsps. amplectens anderadiatus,U. S. A.: Arizona: La Paz Co.: Highway 72 17.5 km east of Salome, 12.5 km W of Maricopa Co. line, R. W. Spellenberg 13138 (NMC), GU246128, GU246191. 9. Thysanocarpus curvipes,intermediate between subsps. amplectensand eradiatus,U. S. A.: Arizona: La Paz Co.: East of Wenden on lower slopes of the Harquahala Mountains ca. 2.81 km northwest of the summit (5,681) of Harquahala Peak, M. D. Windham. 3290A (NMC), GU246129, GU246192. 10.Thysanocarpus curvipes subsp. eradiatus,U. S. A.: Arizona: Mohave Co.: South side of Virgin River Gorge on slopes above Interstate 15 ca. 1.0 road miles east of the lower entrance to The Narrows, M. D. Windham 97-052 (NMC), GU246130, GU246193. 11. Thysanocarpus curvipes subsp. amplectens,U. S. A.: Arizona: Pima Co.: Rincon Mountains, Saguaro National Monument East, Tanque Verde Ridge Trail, C. E. Jenkins 1272 (MO),GU246131, GU246194. 12. Thysanocarpus curvipes subsp. amplectens,U. S. A.: Arizona: Pima Co.: Lower Thomas Canyon, 4 miles southeast of Baboquivari Peak in the Baboquivari Mountains, P. J. Alexander 93 (NMC), GU246132, GU246195. 13. Thysanocarpus curvipes subsp.amplectens,U. S. A.: Arizona: Pima Co.: Coronado National Forest ca. 9.0 miles north of Tucson on the Catalina Highway, H. H. Schmidt 2562 (MO), GU246133, GU246196. 14. Thysanocarpus curvipes subsp. amplectens,U. S. A.: Arizona: Santa Cruz Co.: North side of the Pajarito Mountains on the west side of Peña Bianca Lake, P. J. Alexander 89 (NMC), GU246134, GU246197. 15.Thysanocarpus curvipes subsp. curvipes,U. S. A.: California: Calaveras Co.: Highway 49 1.6 km north of the junction with Hwy. 120, north of Chinese Camp, south side of North Fork of the Calaveras River, R. W. Spellenberg 13504 (NMC), GU246135, GU246198. 16. Thysanocarpus curvipes subsp. curvipes,U. S. A.: California: Colusa Co.: Goat Mountain Road at Sullivan Creek, 1.2 miles southwest of Digger Pine Flat, south of Stonyford, V. H. Oswald 6064 OEPS), GU246136, GU246199. 17. Thysanocarpus curvipes subsp. eradiatus,U. S. A.: California: Imperial Co.: Southwest of Ocotillo in the Jacumba Mountains near Interstate 8 ca. 1.35 km east-northeast of Mountain Spring, M. D. Windham 3295 (NMC), GU246137, GU246200. 18. Thysanocarpus curvipes subsp. elegans,U. S. A.: California: Mendocino Co.: 8 km south of Yorkville, G. L. Smith 6490 (CAS), GU246138, GU246201. 19. Thysanocarpus curvipes subsp. elegans,U. S. A.: California: San Diego Co.: Southeast of Julian along Sunrise Highway near Lake Cuyamaca ca. 2.80 miles east-southeast of the summit (5,593) of North Peak, M. D. Windham 2948 (NMC), GU246139, GU246202. 20. Thysanocarpus curvipessubsp. elegans,U. S. A.: California: Sutter Co.: South side of South Butte, Sutter Buttes, L. Ahart 2726 (CAS), trnL-F only: GU246140, GU246203. 21.Thysanocarpus curvipes subsp. elegans,U. S. A.: California: Tehama Co.: BLM parcel along Highway 36, east edge of Paynes Creek near its mouth on the Sacramento River, Hog Lake Plateau, V H. Oswald 7332.1 (JEPS), GU246141, GU246204. 22. Thysanocarpus curvipes subsp. longistylus,U. S. A.: California: Tuolumne Co.: Along north bank of North Fork of Tuolumne River, 2.9 miles from Long Barn, I. L. Wiggins 21272 (DS), GU246142, GU246205. 23. Thysanocarpus curvipes subsp. longistylus,U. S. A.: California: Tuolumne Co.: Carnegie Institute of Washington Field Station at Mather, F. Almeda 4365 (CAS), GU246143, GU246206. 24. Thysanocarpus curvipes subsp. curvipes,U. S. A.: Idaho: Ada Co.: Freestone Creek, northeast of Boise, B. Ertter 3231 (CAS), GU246144, GU246207. 25. Thysanocarpus curvipes subsp. amplectens,U. S. A.: New Mexico: Luna Co.: A small canyon on the east side of the narrow central portion of the Florida Mountains, just south of Lobo Draw, P. J. Alexander 188 (NMC), GU246145, GU246208. 26. Thysanocarpus curvipes subsp. eradiatus,U. S. A.: Nevada: Clark Co.: West of Las Vegas along Pine Creek Canyon in the Spring Mountains ca. 0.31 km north-northeast of Pine Creek Spring, M. D. Windham 98–118 (NMC), trnL-F only: GU246209. 27. Thysanocarpus curvipes subsp.curvipes,U. S. A.: Nevada: Humboldt Co.: Calico Mountains, A. Tiehm 13112(CAS), GU246146, GU246210. 28. Thysanocarpus curvipes subsp. curvipes,U. S. A.: Nevada: Washoe: Virginia Mountains, Paiute Canyon at south end of the range, 0.4 road miles north of main foothill road, A. Tiehm 13778 (CAS), GU246147, GU246211. 29. Thysanocarpus curvipes subsp. curvipes,U. S. A.: Nevada: Washoe: Steamboat Hot Springs, J. T. Howell 48663 (CAS), GU246148, GU246212. 30. Thysanocarpus curvipes subsp. eradiatus,U. S. A.: Utah: Washington Co.: Southeast slope of the Pine Valley Mountains above Leeds Creek ca. 2.78 km south of Italian Spring, M. D. Windham 97-056 (NMC), GU246149, GU246213. 31. Thysanocarpus curvipes subsp. curvipes,U. S. A.: Washington: Klickitat Co.: Near Klickitat Co. landfill, R. R. Halse 3394 (UC), GU246150, GU246214. 32. Thysanocarpus desertorum,U. S. A.: California: Los Angeles Co.: East of Pinyon Hills, Highway 138 junction with Highway 18, S.Boyd 1540 (UC), ITS only: GU246151. 33. Thysanocarpus desertorum,U. S. A.: California: Los Angeles Co.: Big Rock Creek ca. 0.25 miles south of Big Pines Highway, between the stream and Big Rock Creek Road; Valyermo, T. S. Ross 6100 (CAS), GU246152, GU246215. 34. Thysanocarpus desertorum,U. S. A.: California: Riverside Co.: Joshua Tree National Monument, Squaw Tank, J. H. Thomas 18477(DS), GU246153, GU246216. 35. Thysanocarpus erectus,Mexico: Baja California: Mcpio. Ensenada: 55 miles southeast of San Luis Gonzaga, J. Henrickson 19339(RSA), GU246154, GU246217. 36. Thysanocarpus erectus,Mexico: Baja California: Mcpio. Ensenada: 22 miles northeast of El Arco, on the road to San Francisquito, S. Boyd 5756 (RSA), GU246155, GU246218. 37. Thysanocarpus erectus,Mexico: Baja California: Mcpio. Ensenada: East of La Ramona, S. Boyd 5381 (RSA), GU246156, GU246219. 38. Thysanocarpus erectus,Mexico: Baja California: Mcpio. Ensenada: North of Loma Creston Prieto, S. Boyd 5441 (RSA),trnL-F only: GU246220. 39. Thysanocarpus laciniatus,U. S. A.: California: Alameda Co.: Southeast of Livermore, along Mines Road (Arroyo Mocho) ca. 13.5 miles south of the junction with Tesla Road, L. R. Heckard 2507 (JEPS), GU246157, GU246221. 40. Thysanocarpus laciniatus,U. S. A.: California: Kern Co.: Flying Dutchman Creek canyon on Havilah Mountain Breckenridge road, J. T. Howell 47794 (CAS), GU246158, GU246222. 41. Thysanocarpus laciniatus,U. S. A.: California: Los Angeles Co.: North of Azusa along West Fork of the San Gabriel River ca. 1.51 km north of Fern Springs, M. D. Windham 98–103 (NMC), 41A: GU246160, 41B: GU246159; GU246223. 42. Thysanocarpus laciniatus,U. S. A.: California: Los Angeles Co.: San Francisquito Canyon, westerly draw draining from ridge which divides S. Fr. Cyn. from Dry Canyon; ca. 630 m south of where Drinkwater Canyon joins S. Fr. Cyn., T. S. Ross 7472 (CAS), GU246161, GU246224. 43. Thysanocarpus laciniatus,U. S. A.: California: Los Angeles Co.: San Clemente Island, east side of the island near Malo Point, first canyon south of Bryce Canyon, S. Boyd 4301 (CAS), GU246162, GU246225. 44. Thysanocarpus laciniatus,U. S. A.: California: Orange Co.: Along tributary of Hot Spring Canyon in the Santa Ana Mountains ca. 2.91 km southeast of Crow Spring, M. D. Windham 98-095 (MO), 44A: GU246163, 44B: GU246164; GU246226. 45. Thysanocarpus laciniatus,U. S. A.: California: San Bernardino Co.: North-northwest of Yucca Valley in Water Canyon along Pioneertown Rd. ca. 1.5 road miles north-northwest of its junction with CA Highway 62, M. D. Windham 2957A (NMC), GU246165, GU246227. 46. Thysanocarpus laciniatus,U. S. A.: California: San Diego Co.: West of Campo along old CA Highway 94 on slope above Campo Creek ca. 2.03 km north of VABM 3053 on the US-Mexico border, M. D. Windham 3424 (NMC), 46A: GU246166, 46B: GU246167; GU246228. 47. Thysanocarpus laciniatus,U. S. A.: California: Santa Barbara Co.: Santa Cruz Island, mouth of Dick's Harbor, S. A. Junak SC-1473 (UC), 47A: GU246169, 47B: GU246168; GU246229. 48.Thysanocarpus laciniatus,U. S. A.: California: Sutter Co.: South Butte, Sutter Buttes; along the edge of the road to the lower radio tower on the South Butte, L. Ahart 4637 (CAS), 48A: GU246170, 48B: GU246171, GU246230. 49.Thysanocarpus laciniatus,U. S. A.: Nevada: Nye Co.: Northwest of Rhyolite, M. J. Williams 78-37-1 (CAS), 49A: GU246172, 49B: GU246173, GU246231. 50.Thysanocarpus radians,U. S. A.: California: Butte Co.: Circa 2 air miles north-northeast of Honcut between Oroville and Marysville, Peter Ahart Ranch, B. Ertter 5884 (UC), GU246174, GU246232. 51. Thysanocarpus radians,U. S. A.: California: Napa Co.: 6 miles north of Monticello, C. Best 914 (CAS), GU246175, GU246233. 52. Thysanocarpus radians,U. S. A.: California: Sacramento Co.: Intersection of Bradshaw and Shelton Roads, ca. 3 miles northeast of Elk Grove, G. H. True 6516 (CAS), GU246176, GU246234. 53. Thysanocarpus radians,U. S. A.: California: Tehama Co.: Northwest side of Sacramento Valley, ca. 0.75 miles south of Gyle Road, V. H. Oswald 7240 (MO), GU246177, GU246235. 54.Thysanocarpus rigidus,U. S. A.: California: Riverside Co.: SSW of Palm Desert along CA 74 near rim of Deep Canyon ca..57 km SSW of the summit (4775) of Sugarloaf Mountain, M. D. Windham 3299 (NMC), GU246178, GU246236. 55.Thysanocarpus rigidus,U. S. A.: California: San Bernardino Co.: New York Mountains; east slope of New York Peak, R. F. Thorne 44490 (UC), GU246179, GU246237. Other Thelypodieae—56. Caulanthus inflatus S. Watson, AF531617, AY958534.57. Caulanthus lasiophyllus Payson, AF346644, AY958535. 58. Chilocardamum castellanosii (O. E. Schulz) Al-Shehbaz, AY958592, AY958548. 59.Coelophragmus auriculatus O. E. Schulz, AF531598, AY958547. 60.Dryopetalon runcinatum A. Gray, AF531634, AY958536. 61. Hesperidanthus argillaceus (S. L. Welsh & N. D. Atwood) Al-Shehbaz, AF531611, AY958542.62. Hesperidanthus linearifolius (A. Gray) Rydb., AF531612, AY958540. 63.Mostacillastrum andinum (Phil.) Al-Shehbaz, AF531650, AY958546. 64.Mostacillastrum commune (Speg.) Al-Shehbaz, AF531599, AY958564. 65.Mostacillastrum gracile (Wedd.) Al-Shehbaz, AY958596, AY958554. 66.Mostacillastrum leptocarpum (Hook. & Arn.) Al-Shehbaz, AF531632, AY958556.67. Mostacillastrum subscandens (Speg.) Al-Shehbaz, AF531631, AY958567. 68.Neuontobotrys frutescens (Gillies ex Hook. & Am.) Al-Shehbaz, AY958595, AY958551. 69. Polypsecadium effusum (O. E. Schulz) Al-Shehbaz, AY958594, AY958550. 70. Polypsecadium magellanicum (Pers.) Al-Shehbaz, AF531589, AY958558. 71. Romanschulzia arabiformis (DC.) Rollins, AF531635, AY958538.72. Romanschulzia costaricensis (Standi.) Rollins, AF531636, AY958539. 73.Sibara laxa Greene, AF531637, AY958543. 74. Stanleya pinnata (Pursh) Britton, AF531620, AY122459. 75.* Streptanthella longirostris,U. S. A.: Utah: Wayne Co.: West of Teasdale at N base of Boulder Mtn. ca. 4.04 km SSW of the summit (7472) of Velvet Ridge, M. D. Windham 97-058 (MO), GU246180, GU246238. 76.Streptanthus campestris S. Watson, AF346646, AY958571. 77. Streptanthus cordatus Nutt. ex Torr. & A. Gray, AF531622, AY958572. 78. Streptanthus glandulosus Hook., AF346652, AY958575. 79. Thelypodiopsis elegans Rydb., AF531638, AY958578. 80. Thelypodiopsis shinnersii (M. C. Johnst.) Rollins, AF531640, AY958579. 81. Thelypodiopsis vaseyi (S. Watson ex B. L. Rob.) Rollins, AF531641, AY958580. 82. Thelypodium flexuosum B. L. Rob., AF531624, AY958582. 83. Thelypodium wrightii A. Gray, AF531615, AY958583. 84. Warea amplexifolia Nutt.,AF531642, AY958584. 85. Warea cuneifolia Nutt.,AF531643,AY958585. 86. Weberbauera peruviana (DC.) Al-Shehbaz, AF531593, AY958562. 87. Werdermannia anethifolia I. M. Johnst., AF531645, AY958586.Other tribes, alphabetically by tribe —88. Aethionema grandiflorumBoiss. & Hohen., DQ249867, AP009367. 89. Lobularia libyca Webb & Berthel., DQ249847, DQ518372. 90. Matthiola incana (L.) W. T. Aiton, AJ628339+AJ628340, DQ180301. 91.* Athysanus pusillus,U. S. A.: Arizona: Gila Co.: Southwest of Globe along road to Pinal Recreation Area in the Pinal Mountains, ca. 4.21 km north-northwest of the summit (7812) of Signal Peak, M. D. Windham 2937 (NMC), GU246183, GU246241. 92. Drdba nemorosa L., AY047676, DQ467041. 93. Boecherastricta (Graham) Al-Shehbaz, AF183114, AF307554. 94.Brassica juncea (L.) Czern., DQ340651, EF426774. 95. Crucihimalaya wallichii(Hook. f. & Thomson) Al-Shehbaz, O'Kane, & R. A. Price, AF137555, DQ310520.96. Barbarea vulgaris W. T. Aiton, AJ232915, AY122453. 97. Cochlearia pyrenaica DC., DQ780946+DQ780947, DQ180269. 98.* Descurainia californicaO. E. Schulz, U. S. A.: Utah: Beaver Co.: Circa 13 miles east-northeast of Milford, Bailey's Spring, S. L. Welsh 13949 (MO), GU246181, GU246239. 99.Neotorularia korolkowii (Regel & Schmalh.) Hedge & J. Léonard, AY353156, AY353230. 100. Sphaerocardamum nesliiforme S. Schauer, AF055195, AF307529. 101. Heliophila coronopifolia L., DQ249846, DQ518369. 102.Hesperis matronalis L., AJ628313+AJ628314, AY546166. 103. Iberis amara L., AJ440311, AY122455. 104. Isatis tinctoria L., AF384104, DQ518370. 105.Lepidium virginicum L., AY662280, DQ821405. 106. Noccaea fendleri (A. Gray) Holub, AY154819, AY154804. 107.* Lyrocarpa coulteri Hook. & Harv., U. S. A.: Arizona: Pima Co.: East of State Route 85 near the base of the Gunsight Hills ca. 7.53 km east-northeast of Armenta Well, M. D. Windham 98-038 (MO), GU246182, GU246240. 108. Sisymbrium irio L., AF531558, AY958555. 109.Smelowskia calycina (Stephan ex Willd.) C. A. Mey., AY230640, DQ180295.