Taylor Farnetti BSC 307 DNA and RNA Standard Assessment Objectives 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Recall the history of DNA. Describe the components of DNA. Identify DNA in eukaryotic cells. Being able to identify the overall structure of a DNA molecule from a figure. Describe the components of DNA. Explain the process of mitosis. Relate the DNA molecule to chromosome structure. Identifying the structure differences between DNA and RNA . Comparing DNA and RNA based on similarities. Recall what a gene is. Summarize the events of DNA replication. Describe transcription and the editing of DNA. Summarize the events of DNA replication. Relate gene expression to development. Identify and Explain DNAs functions. Identifying components in DNA. Recalling what double helix stands for in relating to the overall structure of DNA . Relate the DNA molecule to the chromosome structure. Recognize the three different types of RNA and be able to identify the functions of the three. 20. Recognize the three different types of RNA and be able to identify the functions of the three. 21. Recognize the three different types of RNA and be able to identify the functions of the three. 22. A. Explain the history of the Hershey-Chase experiment and identify the importance. B. Describe the overall structure of the DNA molecule. C. Describe transcription and the editing of DNA/ Summarize the events of DNA replication/ Tell how DNA differs from RNA Other objectives I would add to my test with more questions that align to them are: summarizing translation, contrast gene mutations and chromosomal mutations, understanding mutations, and identifying the genetic code DNA AND RNA EXAM KEY: Name: ______________________ Period: ______ Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. ____ 1. Avery’s experiments showed that bacteria are transformed by a. RNA b. DNA. c. proteins. d. carbohydrates. ____ 2. Which of the following is a nucleotide found in DNA? a. ribose + phosphate group + thymine b. ribose + phosphate group + uracil c. deoxyribose + phosphate group + uracil d. deoxyribose + phosphate group + cytosine ____ 3. In eukaryotes, DNA a. is located in the nucleus. b. floats freely in the cytoplasm. c. is located in the ribosomes. d. is circular. ____ ____ Figure 12–1 4. Figure 12–1 shows the structure of a(an) a. DNA molecule. b. amino acid. c. RNA molecule. d. protein. 5. A linear stretch of DNA that specifies the sequence of amino acids in polypeptide is called a a. b. c. d. codon. enzyme. gene. protein. ____ 6. During mitosis, the a. DNA molecules unwind. b. histones and DNA molecules separate. c. DNA molecules become more tightly coiled. d. nucleosomes become more tightly packed. ____ 7. Which of the following include all the others? a. DNA molecules b. histones c. chromosomes d. nucleosomes ____ 8. Unlike DNA, RNA contains a. adenine. b. uracil. c. phosphate groups. d. thymine. ____ 9. Which of the following are found in both DNA and RNA? a. ribose, phosphate groups, and adenine b. deoxyribose, phosphate groups, and guanine c. phosphate groups, guanine, and cytosine d. phosphate groups, guanine, and thymine ____ 10. Genes contain instructions for assembling a. purines. b. nucleosomes. c. proteins. d. pyrimidines. Modified True/False Indicate whether the sentence or statement is true or false. If false, change the identified word or phrase to make the sentence or statement true. ___F_ 11. The replication of a DNA molecule results in four copies of the same gene. __Two_______________________ __T__ 12. The synthesis of an RNA molecule from a DNA template strand is transcription. ____________________ _F___13. In eukaryotes, DNA replication proceeds in one direction along the DNA molecule.___two directions___ _F____14. Genes determine a person’s eye color by coding for nitrogenous bases that affect eye color. _proteins__ _T____15. DNA codes for DNA polymerase. ______ Fill in the Blank Complete each sentence or statement. 16. The structure labeled X in Figure 12–1 is a(an) _nucleotide__. 17. The Watson and Crick model of DNA is a(an) _double helix__, in which two strands are wound around each other. 18. Chromatin contains proteins called __histones__. Matching. Match each of the following words with the correct statements. 19. Carries amino acids to ribosomes, where amino acids are linked into the primary structure of a polypeptide. 20. When a gene transcription occurs, the following is produced. A. mRNA- 20 B. rRNA- 21 C. tRNA- 19 21. This is the cell’s essential protein factory. Essay Portion From the following questions, pick one and answer them in a paragraph format. 22. A. Describe the Hershey-Chase experiment. Why were the results important? Hershey and Chase grew bacteriophages in cultures containing two radioactive isotopes. One became incorporated into the bacteriophage’s DNA, because DNA contains phosphorus. The other one became incorporated into the bacteriophage’s protein coat, because proteins contain sulfur. After the bacteriophages were allowed to infect bacteria, Hershey and Chase found that nearly all the radioactivity in the bacteria was from 32P. This indicated that the bacteriophage’s DNA was injected into the bacteria. The results were important because they showed that the bacteriophage’s genetic material was DNA, not protein. B. Describe the structure of a DNA molecule. A DNA molecule has the shape of a double helix, or that of a twisted ladder. Each strand of the helix is a chain of nucleotides. The two strands are held together by hydrogen bonds between the nitrogenous bases of the nucleotides on opposite strands. The nitrogenous bases form hydrogen bonds with one another in pairs. Adenine forms hydrogen bonds with thymine, and guanine forms hydrogen bonds with cytosine. C. How does transcription differ from DNA replication? Describe at least four differences. RNA polymerase is involved in transcription, whereas DNA polymerase is involved in DNA replication. During transcription, free nucleotides base pair with the nucleotides on only one strand of a DNA molecule, not both strands as in DNA replication. In transcription, the free nucleotides are RNA nucleotides, not DNA nucleotides. Transcription continues until a stop signal is reached on the DNA strand. DNA replication continues until the entire chromosome is replicated. At the end of transcription, one single-stranded RNA molecule is formed, not two double-stranded DNA molecules. The newly formed RNA molecule leaves the nucleus, whereas the newly formed DNA molecules stay in the nucleus.