Course Number : BIO 269-A
Course Title : Basic Cell Culture Lecture
Division/Discipline : Trade & Technology
Credit s: 3 Total Length in Hours : 51
Lecture hours: 17
Lab hours: 34
Course Description :
This course serves as an introductory course for biology, science, and health science majors. Provides an introduction to concepts of animal cell and plant tissue culture, genetic engineering of animal and plant cells in culture, antibody production in mammalian cells, scale – up procedures, industrial uses of cell culture, introduction to laboratory design and layout of cell culture lab, demonstration and experiences with usage of equipment along with maintenance of various aseptic techniques, media formulations and preparations, culturing primary and sub cultures, plant transformation, etc.
Course Competencies/Objectives :
Learning goals for the class include:
1. Explain the importance of animal cell and plant cell cultures, and their advantages and disadvantages.
2. Explain the characteristics of cells and their culture environment in animal and plant culture
3. Describe growth and maintenance of cells in animal and plant culture
4. Discuss and demonstrate genetic engineering concepts like transformation, cloning, selection etc
5. Discuss about scale-up technology (Bioreactors) in animal cell culture.
Learning goals for the lab include:
1. Explain cell culture laboratory lay out
2. Demonstrate usage of different equipment including cell culture microscopes like Inverted and Fluorescence
3. Demonstrating various culturing techniques
4. Demonstrating preparation of various media and antibiotic stock solutions
5. Demonstrating scale-up technology (Bioreactors) used in animal cell culture experiments.
6. Explaining and applying methodology of transformation, cloning and selection in plant tissue culture
Text and Materials :
1. Culture of Animal Cells – A Manual of Basic Technique, 5 th edition (2008), Freshney, John Wiley & Sons, Inc.
2. Animal Cell Culture and Technology – The Basics, 2 nd edition (2008), Butler, Bios Scientific Publishers
3. Plant Cell Culture – The Basics, Evans, Coleman and Kearns (2008), Bios Scientific Publishers
Instructor/Division : Shirisha Yelamanchili, Trade &Technology Date developed : 7-28-08

Course Number : BIO 269-A – Basic Cell Culture Page 2
Lecture component: 1 hour
Name of the chapter
Biology of cultured cells
Media & Supplements, Serum-free media
Refer to the book written by author
Freshney
Freshney
Number of Chapter
3
9, 10
Characterization
Differentiation
Quantitation
Genetic engineering of animal cells in culture
Freshney
Freshney
Freshney
Butler
16
17
21
6
Mammalian cell products
Culture of tumor cells
Introduction and applications of tissue culture
Lecture + Lab component: 2 hours
Name of the chapter
Butler
Freshney
Evan, Coleman & Kearns
Refer to the book written
12
24
1, 14, 15
Number of chapter
Introduction by author
Freshney 1
Freshney 4, 5, 6, 7 Laboratory design and layout, Aseptic technique, Equipment, Safety, bioethics and validation (labs 1 and 2)
Freshney 9, 10 Media & Supplements, Serum-free media,
Preparation and sterilization (lab 3)
Primary culture (lab 4)
Subculture and cell lines (lab 5)
Contamination and Cytotoxicity (labs 6 and 7)
Freshney
Freshney
Freshney
12
13
19, 22
Hybridoma –sources of antibodies
(lab 8)
Transformation and Immortalization (Lab 9)
Scale up (Lab 10 and 11)
Plant transformation (Lab 12, 13 and 14)
Butler
Freshney
Freshney
Evan, Coleman & Kearns
8
18
8, 26
*
Final exams and presentation
* - Materials will be provided in class
Note: This outline is tentative and may be adjusted if needed. Office hours will be decided in the class.

Course Number/Title : BIO269-A - Basic Cell Culture
Course Requirements/Grading Criteria
Student requirements:
1. Exams
Page 3
2. Attendance and participation
3. Completion of assigned readings and exercises
4. Regular quizzes (can be announced or unannounced)
5. Student Presentations
Evaluation and Grading :
Exams may cover material from lecture, textbook, handouts, and overlapping labs. In addition to the unit exams, there will be a student presentation on the last day of the class.
Homework and due dates will be given in class. Assignments are due at the beginning of the lecture period on the due date. Any assigned work which is turned in after a deadline will be subject to a 20% reduction in points for each day late.
Students are expected to keep track of their progress in the course. Students should record the results of all quizzes, homework assignments, and exams immediately after the instructor returns them.
Division of points :
Quiz – 100 points
Quarter term exam: 50 points
Midterm exam: 50 points
Class participation and attendance: 20 points
Assignments – 30 points
Student Presentation: 50 points
Final exam: 50 points
Home work: 50 points
Maintaining cell cultures – 100 points
Note: This division of points is tentative and may be adjusted if needed.
Final Grades : letter grades are assigned based on total points.
A = 90-100% of the total points
B = 80-89% of the total points
C = 70-79% of the total points
D = 60-69% of the total points
F = < 60% of the total points
Make-up Exams/Quizzes (should have a strong reason to take a make-up exam)
No makeup quizzes are given unless you have prior instructor approval and take the quiz in advance of your absence. If you are going to miss an exam you must notify the instructor before the exam is given . Notification is required on my office phone or in person. If you do not notify the instructor before the exam, you will not be allowed to make up the exam. Student absent from an exam may be allowed to take a make-up exam at the discretion of the instructor. Make-up exams are to be taken before the next scheduled class period to avoid a deduction or a grade of 0%.
Academic Dishonesty :
If academic dishonesty is suspected on a test, quiz, or homework assignment, the following procedure will be used:
1. The person or persons suspected will be confronted.
2.
3.
The person or persons suspected will be allowed to state their case.
If it is determined that academic dishonesty has taken place, a zero will be given on the test, quiz, or homework assignment involved.

Course Number/Title : BIO269-A - Basic Cell Culture Page 4
Attendance and Instructor Expectations :
Regular attendance is absolutely necessary for the successful completion of Basic Cell Culture. Students are expected to attend every class/lab. Students are also expected to be on time and ready to begin class at the scheduled time. It is your responsibility to take notes in class in such a manner as to encompass not just what is on overheads, Power Points, or written on the blackboard, but also what the instructor says. Exams are based on material presented in class and material in the textbook as well as laboratory exercises. If absent, it is the student’s responsibility to obtain information covered on that day of class/lab. The instructor will not give the students a copy of instructor’s lecture notes.
Successful completion of Basic Cell Culture will require the student to play an active role in the learning process. In addition to full attention and participation in the classroom, an intense commitment is expected during the hours out of the classroom. If a student has a question they are encouraged to ask the instructor either during class or outside of class. The instructor can be contacted by:
Telephone: (712) 324-5061ext. 235
(800) 352-4907
Email: syelamanchili@nwicc.edu
Office Room: 520 F
Electronic Grading Information :
The final grade will be determined as outlined in the course syllabus. It is the student’s responsibility to keep track of how they are doing as they proceed through the course, based upon information in the syllabus. Any use of an electronic grade book for NCC courses is limited to providing information on individual assignments, and at any given time may NOT be a comprehensive assessment of your course grade.
AMERICANS WITH DISABILITIES ACT (ADA)
In accordance with the Americans with Disabilities Act, students with documented disabilities can pursue accommodations as needed. To assure that accommodations and or modifications will be available when classes start, students are encouraged to make request as soon as possible. Students who require some modifications of seating, testing, or other course requirements should see the instructor or institution’s counselor.

Course Number/Title : BIO269-A - Basic Cell Culture Page 5
This project is funded by a grant awarded under the President’s Community Based Job Training Grant as implemented by the U.S. Department of Labor’s Employment and Training Administration (CB-15-162-06-60).
NCC is an equal opportunity employer and does not discriminate on the following basis:
 against any individual in the United States, on the basis of race, color, religion, sex, national origin, age disability, political affiliation or belief; and
 against any beneficiary of programs financially assisted under Title I of the Workforce Investment Act of
1998 (WIA), on the basis of the beneficiary’s citizenship/status as a lawfully admitted immigrant authorized to work in the United States, or his or her participation in any WIA Title I-financially assisted program or activity.
This workforce solution was funded by a grant awarded under the President’s Community-
Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of the U.S. Department of Labor. The Department of
Labor makes no guarantees, warranties, or assurances of any kind, express or implied, with respect to such information, including any information on linked sites and including, but not limited to, accuracy of the information or its completeness, timeliness, usefulness, adequacy, continued availability, or ownership. This solution is copyrighted by the institution that created it. Internal use by an organization and/or personal use by an individual for noncommercial purposes is permissible. All other uses require the prior authorization of the copyright owner.