CHEM 12A—Trego
Thin Layer and Column Chromatography
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Partner:
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Date Submitted:
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Abstract
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Part A
Sketch your developed TLC plate in the large box below. Record the Rf values for each spot in the
spaces provided. Do not record Rf values for any contaminates in the standards. Note: not all
unknowns contain three compounds.
Fluorene
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Fluorenone
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Fluorenol
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Reference Mixture 1
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Reference Mixture 2
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Reference Mixture 3
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Unknown Spot 1
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Unknown Spot 2
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Unknown Spot 3
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Explain the relative Rf values for Fluorene, Fluorenone, and Fluroenol in terms of polarity and
structure.
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Composition of Unknown
Part D
Describe the TLC developed for fractions 2 and 3 from the column chromatograph. What do these
results suggest about the purity of the compounds isolated?
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Melting Point Data
Solid isolated in fraction 1
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Solid Isolated in fraction 2
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Comment on what the melting point values indicate about the purity of the compounds isolated from
the column.
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Questions
Respond to questions 1 (a, b, c), 2 (a, b) and 3 (a, b, c)
Question 1a
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Question 1b
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Question 1c
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Question 2a
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Question 2b
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Question 3a
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Question 3b
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Question 3c
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