Name: ______________________ Core: _______ Date: ____________ PROTISTS - AMOEBA AND PARAMECIUM – September 16, 2015 Prokaryotes: a single-celled organism that lacks a membrane-bound nucleus. -DNA is in cytoplasm -Ex: bacteria Eukaryotes: any organism whose cells contain a membrane-bound nucleus, which contains its DNA. -Ex: Plants, Animals, Protists Characteristics of Protists: -All protists have a membrane-bound nucleus (“control center” within the cell). -All protists contain other organelles (membrane-bound structures within a cell that perform specific jobs) -Most live in wet places (oceans, lakes, swamps, damp soils.) -Most are unicellular (made of one cell). -Most have structures that help them move. How do protists get energy? -Autotrophs = get energy from the sun through photosynthesis. -Heterotrophs = get energy by eating other organisms or by decomposing material. -Mixotrophs = can get energy from the sun AND by eating other organisms (“mixing it up”). AMOEBA -Microscopic (small), unicellular (one cell) protozoa (animal-like protist) -Live in fresh water, salt water, in soil, and as parasites in moist body parts of animals. Structures of an Amoeba -Plasma membrane: surrounds cell, allows substances to pass in/out -Nucleus: membrane-bound control center -Food vacuole: digests food -Cytoplasm: jelly-like substance within cell -Contractile vacuole: pumps water out of cell Amoeba Movement and Feeding Pseudopodia: extensions of the cytoplasm that allows amoebas to move and detect food; “false feet.” Also called cytoplasmic streaming. Amoeba Reproduction Amoebas reproduce by a type of asexual reproduction (1 parent) called binary fission. = DNA is replicated and cytoplasm pinches in half to produce 2 identical daughter cells. PARAMECIUM Paramecium is a microscopic (very small), unicellular (one cell) protozoa (animal-like protist). It is plentiful in freshwater ponds. Structures of an Amoeba Nucleus: “control center” of a cell. Pellicle: thick membrane that give the paramecium its shape Cytoplasm: jelly-like substance that holds all the organelles. Cilia: hair-like projections that act like microscopic oars Contractile vacuole: Remove excess water out of the cell; works like a pump. Oral groove: takes in food Food vacuole: digests food Anal pore: Removes waste Paramecium Movement -The outer surface of the cell is covered with many hundreds of tiny hair-like structures called cilia. -Act like “microscopic oars” -Can swim backwards, forwards, or rotate using the cilia. -Moves so quickly that we have to add a thickening agent or quieting solution to the slide to slow it down to study it. Paramecium Feeding -The paramecium rotates on its axis as it moves through the water. -Small particles of debris and food are collected and swept into the oral groove (6) and down the funnel-shaped gullet (7) by the cilia that also helps it move. -Eats small organisms such as bacteria, yeasts, algae and even other smaller protozoa. Paramecium Reproduction -Paramecium reproduce by conjugation and binary fission. -Conjugation: 2 paramecia exchange nuclear material and then separate. -Binary fission: cell replicates its DNA, cytoplasm pinches in half, and 2 new daughter cells are formed (cell then grows in size and makes structures). Paramecium Excretion -Food waste left in a food vacuole is excreted through the anal pore (the vacuole and pore fuse. -Other wastes left over from cellular activity (metabolic waste) simply diffuse through the pellicle. -Excess water and some metabolic wastes are excreted through the contractile vacuole.