Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ Matching: Place the letter of the word that best answers the question on the blank. Letters may be used once, more than once or not at all 1. _______ matches amino acids to the growing polypeptide 2. _______ process of rewriting DNA into RNA, takes place inside the nucleus 3. _______ nitrogen base that binds with adenine in RNA 4. _______ any error in the DNA sequence, or gene expression 5. _______ 3-nucleotide sequence on the tRNA that matches mRNA 6. _______ coded message copied from DNA that leaves the nucleus 7. _______ building blocks of proteins 8. _______ five carbon sugar found in RNA 9. _______ cause of a mutation A. amino acid B. anticodon C. codon D. chromosome mutation E. deletion F. frameshift mutation G. inversion H. mRNA I. mutagen J. mutation K. nondisjunction L. peptide bond M. point mutation N. polypeptide O. promoter P. protein synthesis Q. ribose R. rRNA S. RNA T. RNA polymerase U. substitution V. tRNA W. termination signal X. transcription Y. translation Z. translocation AA. uracil 10. _______ frameshift mutation where a nucleotide has been removed 11. _______ process of making proteins out of RNA (in general) 12. _______ forms between two amino acids to make a protein 13. _______ chromosome mutation where segment breaks off, flips around and reattaches 14. _______ three nitrogen bases on mRNA, acts as a code 15. _______ process of mRNA being decoded into a protein, done by ribosomes 16. _______ chain of amino acids 17. _______ material that makes up ribosomes 18. _______ general term for any change in a DNA or RNA sequence 19. _______ enzyme responsible for copying DNA onto mRNA 20. _______ ribose+ phosphate + nitrogen base There are three types of RNA listed below. Name each one and tell its function in transcription/translation (protein synthesis) 21. mRNA 1 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ 22. rRNA 23. tRNA There are two steps in transcription, the first step of protein synthesis. Draw/describe each below the number. 24.-25. 26. Where in the cell does transcription take place? There are three steps in translation, the second step of protein synthesis. Draw/describe each below the number. 27.-29. 30. Where in the cell does translation take place? Here is a DNA strand: 5’ A T G G T C A T A C G C T A A 3’ 31. The matching DNA strand is: 32. The matching RNA strand is: 33. The amino acids would be: 2 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ Here is a list of point mutations. Describe each: 34. Insertion 35. Deletion 36. Substitution Here is a list of chromosomal mutations. Describe each: 37. Deletion 38. Duplication 39. Inversion 40. Translocatioin 41. Nondisjunction 3 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ Answer Key__ Name: _ Period: _____ Date: ________________________ Ch 13 Pretest: RNA and Protein Synthesis Matching: Place the letter of the word that best answers the question on the blank. Letters may be used once, more than once or not at all V___ matches amino acids to the 1. ___ A. amino acid growing polypeptide B. anticodon 2. ____X___ process of rewriting DNA into RNA, C. codon takes place inside the nucleus D. chromosome mutation 3. ___AA____ nitrogen base that binds with adenine E. deletion in RNA F. frameshift mutation 4. __J/D___ any error in the DNA sequence, or G. inversion gene expression H. mRNA 5. ___B____ 3-nucleotide sequence on the tRNA I. mutagen that matches mRNA J. mutation 6. ____H___ coded message copied from DNA that K. nondisjunction leaves the nucleus L. peptide bond 7. ____A___ building blocks of proteins M. point mutation N. polypeptide 8. ___Q____ five carbon sugar found in RNA O. promoter P. protein synthesis 9. ____I___ cause of a mutation Q. ribose R. rRNA 10. ___E____ frameshift mutation where a nucleotide S. RNA has been removed T. RNA polymerase 11. ___P___ process of making proteins out of RNA U. substitution (in general) V. tRNA 12. ___L____ forms between two amino acids to make W. termination signal a protein X. transcription 13. ____G___ chromosome mutation where segment Y. translation breaks off, flips around and reattaches Z. translocation 14. ____C___ three nitrogen bases on mRNA, acts as AA. uracil a code 15. ____Y___ process of mRNA being decoded into a protein, done by ribosomes 16. _____N__ chain of amino acids 17. ____R material that makes up ribosomes 18. ____J___ general term for any change in a DNA or RNA sequence 4 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ 19. __T _____ enzyme responsible for copying DNA onto mRNA 20. ____S___ ribose+ phosphate + nitrogen base There are three types of RNA listed below. Name each one and tell its function in transcription/translation (protein synthesis) 21. mRNA messenger- single stranded molecules that carries the instructions from a gene to make a protein (carries a message) 22. rRNA- ribosomal- part of the structure of ribosome, ribosomes are made of rRNA and many proteins 23. tRNA- transfer- transfers amino acids to the ribosome to make protein There are two steps in transcription, the first step of protein synthesis. Draw/describe each below the number. 24.-25 RNA polymerase binds; DNA unwinds mRNA starts to form DNA and mRNA are released 26. Where in the cell does transcription take place? Nucleus There are three steps in translation, the second step of protein synthesis. Draw/describe each below the number. 27.-29. initiation: tRNA binds to mRNA elongation: first peptide bond forms elongation: polypeptide chain grows termination: protein is complete 5 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ 30. Where in the cell does translation take place? Cytoplasm Here is a DNA strand: 5’ A T G G T C A T A C G C T A A 3’ 31. The matching DNA strand is: 3’ T A C C A G T A T A G C G A T T 5’ 32. The matching RNA strand is 5’ A U G G U C A U A C G C U A A 3’ 33. The amino acids would be: start (met)- val- iso- arg- stop 6 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ Here is a list of point mutations/framshift. Describe each: 34. Insertion- addition of a nucleotide and framshift occurs 35. Deletion-loss of a piece of a nucleotide, cause a frameshift 36. Substitution-one nucleotide replaces another Here is a list of chromosomal mutations. Describe each: 37. Deletion- loss of a piece of a chromosome due to breakage 38. Duplication- addition of a sequence 39. Inversion- segments breaks off, flips around and reattaches 40. Translocatioin breaks off and reattaches to non homologous chromosome 41. Nondisjunction- chromosome fails to separate from its homologous during meiosis, get and extra copy (trisomy 21) 7 Ch 13 Pretest: RNA and Protein Synthesis Name: ______________________________ Period: _____ Date: ________________________ 8