Curriculum Vitae
Petroff, Brian K.
Page |1
Date: 3/10/2014
I.
PERSONAL DATA:
1. Applicant Information:
First Name: Brian
Middle Initial: K
Last Name: Petroff
Suffix:
Degree(s): D.V.M., Ph.D.
Current Academic Rank: Associate Professor with Tenure
Primary Department: Internal Medicine
Secondary Department: Physiology
Office Address: WHE 1005, 3901 Rainbow Boulevard, Kansas City, KS 66160
Phone: 913-588-3992
Fax: 913-588-3821
Email: bpetroff@kumc.edu
2. Professional Development:
Undergraduate and Graduate Education:
Years (inclusive)
Degree
1988-1992
B.S. (Anim Sci, summa cum laude)
Institution
Ohio State University
1992-1994
D.V.M. (magna cum laude)
Ohio State University
1996-1998
D.V.M. (magna cum laude)
Ohio State University
1992-1996
Ph.D. (Physiology)
Ohio State University
Degree
Intern, Veterinary Medicine
Women’s Health
Frontiers in Repro Summer
Course
Clinical practicum
Institution
VVC, Pataskala, OH
KUMC, Center for Reprod. Sci.
Woods Hole Biological Laboratory
Postgraduate Education:
Years (inclusive)
1998
1998-2000
2001
2008
Academic and Professional Appointments and Activities:
Month and Year
Position
06/1980-08/1985
Herdsman
08/1985-07/1988
Military Policeman, Team Leader
08/1988-08/1992
Veterinary Assistant
08/1992-06/1996
Graduate Research Assistant
06/1994-06/1998
Veterinary Clinical Assistant
03/1998-09/1998
Veterinarian
09/1998-09/2000
NIH NRSA Research Fellow
09/2000-12/2004
04/2003-present
01/2005-2009
Research Assistant Professor
Laboratory Scientific Director
Assistant Professor
Dr. Jeffery Reiswig
Institution
Spoerr Farms, New London, OH
U.S. Army MP Corps
Wakeman Veterinary Clinic, OH
Reproductive Biology, Ohio State
Veterinary Medicine, Ohio State
Village Vet. Care, Pataskala, OH
Center for Reproductive Sci,
KUMC
Physiology, KUMC
Breast Cancer Preven., KUMC
Internal Medicine, KUMC
Curriculum Vitae
07/2009-present
06/2006-present
Petroff, Brian K.
Associate Professor
Co-leader, Cancer Prevention
Page |2
Internal Medicine, KUMC
KU Cancer Center
Professional Registration/Licensure:
Year
Number
1998-present
Veterinary License 763
OH
2010-present
MO
Veterinary License 2010031563
State
Professional Societies and Affiliations: List by national and local level; include offices held
Date
Organization
1996-present
American Veterinary Medical Association, National
1998-present
Society for the Study of Reproduction, National
1999-2009
Society of Toxicology, National
2002-present
American Association for Cancer Research, National
2008-present
American Society for Fertility Preservation, National
Honors and Awards:
Year
1989
1991
1989-1991
1990
1991
1992-1993, 1997-1998
1993-1997
1997
1998
1998
1999
2000-2004
2001
2004
2005
2007
2008
2009
2010-2015
2011
2013
Award
Ohio State University Undergraduate Research Award
Ohio State University Sigma Xi Undergraduate Research Award
Ohio State University Kottman Honors Scholarship
Top Junior Scholar, Ohio State Univ. Coll. of Agric. and Natural
Resources
Top Senior Scholar, Ohio State Univ. Coll. of Agric. and Natural
Resources
Hills Pet Products Veterinary Scholarship
Ohio State University College of Agriculture Kennedy Fellowship
AVMA Student Chapter Reproductive Physiology Award
Ohio State University Veterinary Medicine Reproductive Medicine
Award
KUMC, NIH Institutional National Research Service Award
NIH Individual National Research Service Award
Mellon CONRAD Junior Investigator Grant
Frontiers in Reproduction Scholarship, Woods Hole Biological
Laboratory
NCI/SPORE Career development award in breast cancer research
Dr. Susan Love Research Foundation pilot award
Nominee, KUMC Chancellor’s Teaching Award
Nominee, Chancellor’s Teaching Award
Panelist, EPA Panel on Health Effects of Dioxin-like Chemicals
Komen Promise grantee, Komen for the Cure
Chancellor’s Distinguished Teaching Award (highest institutional
teaching award)
Komen Zumba Scholar
Curriculum Vitae
II.
Petroff, Brian K.
Page |3
TEACHING ACTIVITIES:
Teaching evaluations and other evidence of quality teaching must be provided and should accurately
summarize ALL of your teaching activities.
Philosophy of Teaching:
Briefly describe your philosophy of teaching and how you apply it to different types of learners, e.g. medical
students, graduate students, residents, fellows.
My teaching interests include translational biomedical and animal science and integrative medicine.
Reproductive cancers, infertility and environmental health case discussions and thought experiments are used
commonly in my graduate lectures. I use problem based learning throughout medical and graduate education
and for undergraduates where appropriate. Additionally, I enjoy teaching scientific history, comparative
human and veterinary medicine and physiology and the theory and practice of biomedical laboratory
techniques. My general teaching approach is to combine strong factual underpinnings with strategies to
engage students as active learners. I adapt this approach to match the goals of the coursework – imparting
concise basic knowledge and problem solving skills for all, emphasizing concepts for undergraduates, applied
and theoretical scientific methodology for graduate students and synthesis of pathophysiology for medical
students.
1. Instruction:
Didactic: e.g. lectures and formal presentations
Instruction
Title and
Academic
Course
Year
Number
2000Reproductive
present
Physiology
2002Med Physiol
2007
Research
2007
techniques
2007Sexuality
present
Reprod. Med.
2012Environmental
present
Toxicology
2013Cancer Biology
present
Learner
Type of
Type (e.g.
Learner(s)
lecture,
(e.g. medical
grand
students,
Lecture or
rounds,
Hours
Number
graduate
Presentation
formal
(actual
of
students,
Title
presentation) instruction) Learners
residents)
Lecture
Lecture
8/year
6
graduate
Case Study
Lecture
12/year
12
medical
Lecture
Lecture
6/year
20
residents/staff
Lecture
3/year
3
120
medical
Lecture
1/yr
1
10
MPH
Lecture
1/yr
1
20
mixed
Curriculum Vitae
Petroff, Brian K.
Page |4
Nondidactic: e.g. workshops, labs, and discussion groups
Instruction
Type (e.g.
small group,
laboratory,
seminar,
Title and
workshop,
Hours
Academic
Course
Presentation
journal
(actual
Year
Number
Title
club)
instruction)
Journal
2005-2007
Discussion
Rounds
Review
1
Problem
2005based
Discussion
PBL
40-48/year
present
learning
Learner
Type of
Learner(s)
(e.g. medical
students,
graduate
Number of
students,
Learners
residents)
20
residents/staff
10
medical
Master’s Theses and PhD Dissertations Directed: List only those for which you are/were primarily
responsible.
Degree
Year
Student Name
Thesis Title
(completed/in progress)
2005-2011
Alison Ting
A combined approach to
Ph.D. (hons), SP 2009
breast and ovarian cancer
prevention
2010-2012
Megan Kreitner
Nutritional prevention of
breast and ovarian cancer
M.S.
2011-present
Brandon Hidaka
Fatty acid profiles and
cancer prevention
M.D. Ph.D. student
Supervision of Postdoctoral Fellows: List only those for which you are/were primary supervisor.
Year
Fellow Name
Area of Study
12/2004Anita Franczak, Ph.D.
Reproductive Endocrinology
06/2005
09/200501/2007
07/20042006/09
Zhanquan Shi, Ph.D.
Reproductive Endocrinology
Kelli Valdez, Ph.D.,
Women’s cancers
Advising: Thesis or dissertation committees; student academic group/individual
Date
Student or Group Name
Type of Student/Group
2006-2008
Stephanie Fiedler, Physiology
Ph.D.
2008-2010
Erik Pacyniac, Pharmacology and
Toxicology
Ph.D.
2008-2011
Ann Thomas, Pharmacology and
Toxicology
Ph.D.
2008-2012
Pan Wang, Pharmacology and Toxicology
Ph.D.
2010-present
Jonathan Fitzgerald, Mol. Int. Physiology
Ph.D.
Curriculum Vitae
Petroff, Brian K.
Page |5
2. Development of Educational Materials:
Describe any process of peer-review or expert assessment to which materials have been subjected.
Specifically document if the materials have been used or referenced by colleagues, especially at other
institutions or otherwise disseminated. Examples of course materials include syllabi, educational software
packages, web sites, films, educational tapes, and evaluation tools.
Year
2000-present
Title Description
Corpus luteum and comparative
endocrinology (new lecture)
Intended Audience
Graduate Students
2005-2006
Theory and practice of
biomedical laboratory
techniques (new lecture series)
M.D. students and housestaff
2007-present
Sexlty Reprod. Med (new
lectures)
1) Fetal origins of adult disease
2) Risk factors breast and
ovarian cancer
Mammary gland and breast
cancer (new lectures)
Normal and assisted parturition
(new lectures)
M.D. students
Reproductive toxicology and
carcinogenesis
One Health website and seminars
MPH students
2008
2008-present
2012
2013
Graduate Students
Graduate Students
M.D., MPH and D.V.M. students
3. Educational Leadership:
Describe administrative responsibility for courses and other leadership activities, including formal
mentoring of junior faculty. Please list faculty members mentored and your role as mentor- letters from
mentorees may be included.
Director and course originator – Principles of laboratory practice lecture series for Internal Medicine students
and housestaff
Member, Ad hoc Ob Gyn curriculum evaluation committee, KUMC, 2008
Lead, KUMC One Health website and coursework, 2013
Curriculum Vitae
III.
Petroff, Brian K.
Page |6
SERVICE ACTIVITIES:
1. Academic Service:
In Academic Service, your contribution to the academic community should be clearly documented. Names
and dates of committees, task forces, or working groups should be provided. A brief description of the
significance of the group and explanation your role should be provided. Activities related to University of
Kansas School of Medicine Academic Societies should be documented in this section.
Chairman: Pharmacology sessions, annual Central Veterinary Conference, Kansas City, MO, 2000-2008
Member, KUMC Institutional Animal Care and Use Committee; 12/03-present
Member, Kansas University Cancer Center, Protocol Review and Monitoring Committee, 2004-present
Member, Clinical Outreach Committee, Society for the Study of Reproduction, 2006-2009
Member, Ad hoc research committee, Department of Internal Medicine, KUMC
Program Co-leader, University of Kansas Cancer Center, Cancer Prevention 06/06-present
Member, Ad hoc Ob Gyn curriculum evaluation committee, KUMC, 2008
Member, Environmental Protection Agency Scientific Review Panel, 2008-2009
Vice Chair, KUMC Institutional Animal Care and Use Committee; 08/08-present
Chair, KUMC Institutional Animal Care and Use Committee; 04/12-12/12
Scientific Director: Breast Cancer Prevention Laboratory, KU Medical Center
Member, Kansas University Cancer Center, Shared Equipment Committee, 2008-present
Member, Kansas University Cancer Center Leadership Council, 2008-present
Member, KUCC ACS Training Grant Advisory Board, 2008-present
Member PI, Center for Reproductive Sciences, University of Kansas Medical Center
Member, Mental Retardation Research Center, University of Kansas Medical Center
Judge, KUMC Student Research Forum, 2002-present
Judge, KUMC Postdoctoral Research Forum, 2009-present
Judge, Kansas Junior Science Association, 2004-present
Mentor, Kansas Science Pioneers
Mentor/Advisee, Ms. Alison Ting, Ph.D., 2008 Top Trainee Presentation, KUMC Student Research
Forum
Ad Hoc Reviewer: Biology of Reproduction, Biomarkers, Biomarkers in Medicine, BMC Cancer, Breast
Cancer: Basic and Translational Research, Cancer Research, Carcinogenesis, Comparative
Biochemistry and Physiology, Congenital Diseases and Environment, Current Medicinal Chemistry,
Endocrine, Endocrinology, Fertility and Sterility, Journal of Assisted Reproduction and Genetics,
Journal of Endocrinology, Marine Drugs, Molecular and Cellular Probes, Reproduction, Reproductive
Biology, Reproductive Biology and Endocrinology, Reproduction Nutrition and Development,
Reproductive Toxicology, Toxicology, Toxicology and Applied Pharmacology, Toxicology Letters,
Zoological Sciences
Ad Hoc Reviewer: Chemoprevention study section, National Cancer Institute, NIH, 10/05, 10/08
Ad Hoc Reviewer: Kansas City University of Medicine and Biosciences Pilot Proposals, 12/05
Ad Hoc Reviewer: Department of Defense IDEA program, 2006; Kansas City Area Life Sciences Inst, 2006
Ad Hoc Reviewer: National Institute of Environmental Health Sciences, 11/08
Ad Hoc Reviewer: US-Israel Binational Agricultural Research and Development Fund, 11/09
Ad Hoc Reviewer: NIH/NIEHS ARRA BPA Grand Opportunity Awards, 2009
Ad Hoc Reviewer: NIH ICER study section, 2010
Ad Hoc Reviewer: NICHD training grants, 2012
North American Managing Editor, Reproductive Biology
Editorial Board: Current Medicinal Chemistry
Curriculum Vitae
IV.
Petroff, Brian K.
Page |7
RESEARCH AND SCHOLARLY ACTIVITIES:
Opening Statement:
Provide a brief opening statement to orient the reviewers to the principal areas of scholarly activity and
provide your perspective. A description of your most significant contributions to the literature is often
helpful to the reviewers.
Cancers of the female reproductive system, endocrine disruption and infertility share a number of
suspected disease pathways and risk factors. My laboratory focuses upon the characterization and
antagonism of promising targets in animal models. Major research efforts include the study of
individuals at high risk for breast or ovarian cancer, the use of novel technologies to assess early
markers of risk for these conditions and characterization and alleviation of the premature loss of
female fertility with age and chemotherapy or toxicant exposure. A novel focus of our work concerns
the impact of selective estrogen receptor modulators on normal and precancerous breast and ovarian
tissue and the follicular reserve. I also supervise the laboratory evaluation of promising chemo
preventives for breast and ovarian cancer in human clinical trials through the KUMC Breast Cancer
Prevention Center. The central goal of this work is to improve reproductive health across species
through improved understanding of endocrine disruption and an emphasis on prevention. Major
research efforts in more detail include:
1. Breast and ovarian cancer prevention: Ovarian cancer prevention faces nearly insurmountable
biological and logistical obstacles due to difficulties in imaging and sampling ovarian tissue and the low
incidence of the disease. Our approach is to develop breast cancer prevention strategies focused on
risk factors in common with ovarian cancer. Towards this end, we have developed a novel animal
model of simultaneous breast and ovarian cancer progression which is used in parallel with our human
breast cancer prevention studies in an effort to prioritize breast cancer prevention strategies giving
simultaneous benefit to ovarian cancer. This work was recognized with a 5 year Komen Promise
Award. Additional work seeks to identify common targets for dual breast and ovarian cancer
prevention efforts. Our goal for this work is to identify and test chemoprevention agents with
simultaneous benefit for breast and ovarian cancers.



Ting, A., B.F. Kimler, C.J. Fabian and B.K. Petroff. 2007. Development of a preclinical model of
simultaneous breast and ovarian cancer progression. Carcinogenesis, 28:130-135.
Ting, A., Fabian, C.J., Kimler, B.F. and B.K. Petroff. 2009 Tamoxifen prevents preneoplastic
changes of the breast but the ovary in a rat model of simultaneous breast and ovarian cancer.
Cancer Prevention Research, 1:546-553.
Petroff, B.K. 2012. A case for combined breast and ovarian cancer prevention. Med. Hypoth,
79:549-551.
2. Human breast cancer prevention trials: Based upon my endocrine disruption biomarker work, I was
recruited into our Breast Cancer Prevention Center at KUMC under the leadership of Dr. Carol Fabian –
perhaps the most successful clinician in human breast cancer prevention in the world. For the past 7
years I have served as the laboratory scientific director of this group, directing both exploratory
endocrine and tissue biomarker analysis of our human clinical trials (including LCM, proteomic,
epigenetic, mRNA and miRNA endpoints), performing parallel preclinical studies and serving as leader
of our large cancer prevention group with Dr. Carol Fabian. Goals for this research going forward
would be to translate this successful human approach into similar efforts in veterinary species.
Curriculum Vitae


Petroff, Brian K.
Page |8
Fabian, C.J., B.F. Kimler, C.M. Zalles, Q.J. Khan, M.S. Mayo, T.A. Phillips, M. Simonsen, T. Metheny
and B.K. Petroff. 2007. Reduction in proliferation with six months of letrozole in women on
hormone replacement therapy. Breast Cancer Res Treat, 106:75-84.
Petroff, B.K., Phillips, T., Kimler, B.F., Fabian, C.J. 2013 Comparison of RNA endpoints in fixed
vs. frozen benign human breast tissue harvested by ductal lavage or random periareolar fine
needle aspiration (RPFNA). 2012. Reprod Biol, 13:75-81.
3. Iatrogenic oocyte loss and endocrine damage: While testing tamoxifen in our animal model of breast
and ovarian cancer, we serendipitously discovered that SERMs can protect the ovarian follicular
reserve from multiple ovotoxicants, including cancer chemotherapy drugs. We are incredibly excited
about this research since tamoxifen has already been used safely in front line cancer treatment, is
relatively inexpensive, non-invasive and avoids treatment delay – unlike current cryopreservation
efforts for fertility preservation. This work has already garnered several awards and NIH pilot funding.
An NCI R21 and March of Dimes application to explore the mechanisms of follicular rescue with SERMs
is currently in review after narrowly missing funding on initial submission. We are also performing a
locally funded teratogenesis study to determine the impact on embryo quality. Future goals for this
work are to better elucidate the therapeutic mechanism of tamoxifen during ovarian protection, to
engage human clinicians and move into human phase I study as appropriate and to apply these insights
into veterinary species. *Most recently this work was featured editorially in the journal Endocrinology
when a European group published their findings of protection from radiation-mediated infertility using
SERMs citing our studies as their incitement.




Ting, A. and Petroff, B.K. 2010. Tamoxifen rescues ovarian follicles from multiple classes of
ovarian toxicants including cancer chemotherapy. J. Assist. Reprod. Genet., 27:519-525.
Albertini D, Fabian, CJ., Ting, A., Kim, S., Petroff, B.K. 2011. Hormonal techniques for fertility
preservation in cancer patients. Clin Ob Gyn, 53:740-752.
Piasecka, J., Delman, D., Ciereszko, R.E., Petroff, B.K. 2013. Tamoxifen prevents chemotherapymediated apoptosis and follicular depletion in vitro. Biol. Reprod., in review.
*Gleicher, N., Kushnir, V.A., Barad, D.H. 2013. Therapeutic internventions into early stage of
follicle maturation: a new treatment paradigm after over 50 years of modern infertility
treatment. Endocrinology 154:3498-3501. Editorial featuring Petroff lab work.
4. Ovarian physiology and endocrine disruption: This work began during my postdoctoral study with
Dr. Paul Terranova and initially focused on ovulation and its disruption by ligands of the aryl
hydrocarbon receptor in the environment. Major contributions in this arena from our group
include documentation of premature menopause from chronic, environmentally-relevant
exposures to aryl hydrocarbon receptor ligands and elucidation of major mechanisms of this
toxicity. This work garnered repeated NIEHS support and was featured at the 2010 SSR meeting in
Milwaukee. Our fertility preservation work is an offshoot of this research as well. Additionally, an
emerging topic, which we are investigating in collaboration with Dr. Margaret Petroff, is the role of
Autoimmune Regulator (AIRE) in immune-mediated premature reproductive failure. We are
resubmitting an RO1 as Co-PIs (initial score 30th percentile). This endocrine disruption work has
already been integrated into veterinary species with several grants and publications in
collaboration with European veterinary faculty.
 Shi Z.Q., Valdez, K.E., Ting, A., Gum, S., Franczak, A. and B.K. Petroff. 2007. Chronic AhR ligand
exposure accelerates reproductive aging via ovarian endocrine disruption at environmentally
relevant exposures. Biol Report, 76:198-202.
 Hutt, K., Shi, Z., Albertini, D.F. and B.K. Petroff. 2008 Aberrant aryl hydrocarbon receptor
activation disrupts morphogenesis of the rat conceptus. BMC Dev Biol, 8:1-12.
 Valdez, K., Shi, Z., Ting, A. and B.K. Petroff. 2009 Gene expression profile accompanying
premature reproductive aging with chonic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Curriculum Vitae
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
Petroff, Brian K.
Page |9
Reprod. Toxicol., 28:32-37.
Jablonska, O., Shi, Z., Valdez, K.E., Ting, A. and B.K. Petroff. 2010. Temporal and anatomical
sensitivities to the aryl hydrocarbon receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin
leading to premature acyclicity with age in rats. Int. J. Androl., 33:405-412.
Jasti, S., Warren, B., McGinnis, L.K., Kinsey, W.H., Petroff, B.K./M.G. (shared senior authorship)
2012. The autoimmune regulator (AIRE) prevents premature reproductive senescence in
female mice. Biol Reprod, 12:86-92. Editorial: Cushman, R.A.
doi:10.1095/biolreprod.112.099242.
While the work of my laboratory has been varied, the common theme has been female reproductive health
and endocrine disruption.
1. Grants and Contracts:
Information must include whether you are the principal investigator or a co-investigator, names of all
investigators, title of grant, funding source, dollar amount in direct costs, and years during which grant
applies. Co-investigators must specify role. Provide the cover sheet, abstract, and Notice of Award in PDF
for all grants or contracts awarded in last five years.
Previous Grants and Contracts Awarded: List in chronological order; oldest first, newest last; list the
applicant’s name in BOLD.
Inclusive
Principal
Title of
Direct
Years of
Investigator Investigators Grant/Contract Funding Source
Costs
Award
Status
Petroff
Novel
Mellon
2000 compounds that
Foundation/
2003
block follicular
CONRAD
rupture
Fabian
Petroff
A study to
N01 CN 151352001 Kimler
identify
MAO NIH/NCI
2006
biomarker
modulation by a
COX-2 inhibitor
in breast tissue of
premenopausal
women at high
risk for estrogen
receptornegative breast
cancer
Petroff
Toxigenomic
KCALSCI-02-09
2002 profile of dioxin
Kansas City Area
2003
in the aging
Life Sciences
female
Institute
Petroff
The aryl
National
2003 Ciereszko
hydrocarbon
Research
2004
receptor and
Council/National
prolactin
Science
pathways
Foundation
Curriculum Vitae
Petroff, Brian K.
Fabian
Petroff
Fabian
Petroff
Petroff
Petroff
Study of the
effect of letrozole
on breast
biomarkers of
high risk
postmenopausal
women receiving
hormone
replacement
therapy
Phase IB study of
biomarker
modulation of a
cyclooxygenase-2
inhibitor vs.
placebo in newly
diagnosed breast
cancer
Simultaneous
chemoprevention
of breast and
ovarian cancer
NIH Breast
SPORE Career
development
award
P a g e | 10
CFEM345A US06
Novartis
Pharmaceutical
Company
2002 2005
NQ8-01-012
PharmaciaPfizer
2002 2006
3 P50
CA089019-05S6
Avon-NCI
Progress for
Patients Award
Program via
2003 2005
NIH/NCI
Subcontract
from the
University of
Alabama
Birmingham
SPORE in Breast
Cancer
3 P50
CA089019-05S6
Avon-NCI
Progress for
Patients Award
Program via
NIH/NCI
Subcontract
from the
University of
Alabama
Birmingham
SPORE in Breast
Cancer
2004 2005
Curriculum Vitae
Petroff, Brian K.
Petroff
Petroff
Petroff
Fabian
Bland
Petroff
Fabian
Petroff
Fabian
Petroff
Albertini
Petroff
Petroff
Petroff
Fabian
Petroff
Laser capture
and real time
PCR to assess
breast biomarker
gene expression
following RPFNA
and ductal lavage
Clinical trial
model for
ovarian cancer
chemoprevention
Aryl
hydrocarbon
receptor
pathway and the
aging female
SPORE in Breast
Cancer
Flaxseed lignan
for breast cancer
chemoprevention
Breast cancer
prevention by
letrazole in high
risk women
Endocrine
disruption and
embryonic stem
cell
morphogenesis
Breast stem cells
during benign
breast disease
AhR and
reproductive
aging
Breast cancer
prevention by
letrozole in high
risk women
P a g e | 11
Dr. Susan Love
Research
Foundation
2004 2006
KUMC Research
Institute
2004 2006
5-RO1
ES012916
NIH/NIEHS
2004 2008
3 P50
CA089019-05S6
Avon-NCI
Progress for
Patients Award
Program via
NIH/NCI
Subcontract
from the
University of
Alabama
Birmingham
R21 CA11784701 NIH/NCI
2005 2008
CFEM345DUS45
Novartis
Pharmaceuticals
Corporation
Kansas City Area
Life Sciences
Institute
2006 2009
RO3 CA12141702 NIH/NCI
2007 2009
1 R21
ES015879-01
NIH/NIEHS
1 RO1
CA122477-01A1
NIH/NCI
2007 2010
2005 2009
2007 2009
2007 2011
Curriculum Vitae
Petroff, Brian K.
Petroff
Petroff, MG
Fabian
Petroff
Petroff
Petroff
Petroff
Petroff
Petroff, MG
Center immune
tolerance,
autoimmunity
and infertility
Biomarkers of
Response to
Prevention
Strategies for
Postmenopausal
Women
Fertility
preservation in
women with
cancer
A rat model of
inducible p53
driven
tumorigenesis
Testing a Novel
Benefit to
Children from
Altered Maternal
Estrogen
Signaling
Placental
immunology and
cancer risk
P a g e | 12
Lied Pilot Grant
Program
2008 2009
Breast Cancer
Research
Foundation
2008 2009
Breast cancer
prevention by
letrozole in high
risk women
Kansas Masonic
Cancer Research
Institute
2008 2011
Kansas City Area
Life Sciences
Institute
2012 2013
Lied Biomedical
Sciences Grant
2012 2013
2010 2011
Current Grants and Contracts Awarded: List in chronological order; oldest first, newest last; list the
applicant’s name in BOLD.
Inclusive
Principal
Title of
Funding
Direct
Years of
Investigator Investigators Grant/Contract
Source
Costs
Award
Status
Fabian
Petroff
Biomarkers of
Breast
2008 Response to
Cancer
2014
Prevention
Research
Strategies for
Foundation:
Postmenopausal
Women
Fabian
Petroff
Vitamin D for
1 R01
2009 prevention of
CA1388862014
breast cancer in 01 NIH/NCI
premenopausal
women
Petroff
Cancer
Komen
2010 Fabian
Prevention with Promise
2015
Flaxseed Lignan Program
Grant
Jensen
Program
This center
1 P30
2012 Leader –
grant
CA1685242017
Curriculum Vitae
Petroff, Brian K.
Petroff
Petroff
Petroff, MG
Petroff
establishes
University of
Kansas Cancer
Center as a NCIdesignated
matrix cancer
center and
provides
support for
administrative
and core facility
for cancer
research at
KUMC
Combined
Prevention of
Breast and
Ovarian Cancer
Exosomes and
Maternal-Fetal
Interaction
P a g e | 13
01
University of
Kansas
Cancer
Center
University of
Kansas
Medical
Center
NIH ACB
COBRE Pilot
Grant
2013 2014
2014
Petroff,
MG
Grants and Contracts Submitted: List in chronological order; oldest first, newest last; list the applicant’s
name in BOLD.
Inclusive
Principal
Title of
Funding
Direct
Years of
Investigator Investigators Grant/Contract
Source
Costs
Award
Status
Petroff B.K. Fabian,
Protection
1
275,000
2013 –
ReKimler,
against
R21CA1826902015
submit
Sinclair,
chemotherapy01
July 2014
Hagenbuch
induced ovarian
toxicity
Petroff B.K. Fabian,
Ovarian
March of
300,000
2014 Pending
Kimler,
estrogen
Dimes
2016
Sinclair,
signaling and
Hagenbuch
birth defects
Petroff B.K. Vielhauer,
Maternal
Department of 320,000
2014 –
Pending
Fabian,
immunity and
Defense
2016
Kimler,
ovarian cancer
Petroff M.
prevention
Petroff B.K. Vielhauer,
Biopanning for
Department of 320,000
2014 –
Pending
Fabian,
autoantibodies
Defense
2017
Kimler
during breast
cancer
progression
Petroff B.K. Petroff M.,
A rational
KU Cancer
35,000
2014
Pending
Ellerbeck,
approach to
Center
Culley,
prevention
Yankee
biomarker
development
Curriculum Vitae
Petroff M.G.
Petroff, Brian K.
Petroff B.K.
Placental cancer
antigens and
cancer risk
National
Cancer
Institute
P a g e | 14
275,000
20142016
Pending
2. Scholarly Publications:
Publications must be numbered in chronological order, oldest publication first, most recent publications
last. In cases where there are multiple publications within a year, they should be listed in alphabetical
order.
Articles published: Include full-length, peer-reviewed articles in scientific journals; this list should not
include papers in preparation, submitted, or under revisions, nor should it include conference
proceedings, published abstracts, and book reviews (which are listed separately). Provide names of all
authors (applicant’s name in BOLD), year, title, journal, volume, and inclusive pages.
1. Inpanbutr, N., E.K. Miller, B.K. Petroff, and A.M. Iacopino. 1994. CaBP-D9k levels during the
luteal and follicular phases of the estrous cycle in the uterus. Biol. Reprod. 50:561-571.
2. Ciereszko, R.E., Z. Guan, B.T. Stokes, B.K. Petroff, et al. 1995. Arachidonic acid inhibits hCGstimulated progesterone production by corpora lutea of primates: potential mechanism of
action. Prostaglandins 50:103-110.
3. Petroff, B.K., K. Dabrowski, R.E. Ciereszko and J.S. Ottobre. 1997. Total ascorbate and
dehydroascorbate concentrations in porcine ovarian stroma, follicles and corpora lutea
throughout the estrous cycle and pregnancy. Theriogenology 47:1265-1273.
4. Petroff, B.K., R.E. Ciereszko, K. Dabrowski, A.C. Ottobre, W.F. Pope and J.S. Ottobre. 1998.
Depletion of vitamin C from pig corpora lutea by prostaglandin F2a-induced secretion of the
vitamin. J. Reprod. Fert. 112:243-247.
5. Ciereszko, R.E., B.K. Petroff, A.C. Ottobre, Z. Guan, B.T. Stokes and J.S. Ottobre. 1998.
Assessment of the mechanism by which prolactin stimulates progesterone production by early
corpora lutea of pigs. J. Endocrinol. 159:201-209.
6. Petroff, M.G., B.K. Petroff, and J.L. Pate. 1999. Expression of cytokine messenger mRNAs in the
bovine corpus luteum. Endocrinology 140:1018-1021.
7. Petroff, B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2000.
Effects of 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) and estradiol cypionate in an ovulation model: evidence
for local interaction and systemic effects. Reprod. Toxicol. 14:245-255.
8. Gao, X, B.K. Petroff, K.K. Rozman and P.F. Terranova. 2000. Gonadotropin releasing hormone
(GnRH) reverses the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin in the ovary of immature
Sprague-Dawley rats. Toxicology 147:15-22.
9. Petroff, M.G., B.K. Petroff, and J.L. Pate. 2001. Mechanisms of cytokine-induced cell death of
cultured bovine luteal cells. Reproduction 121:753-760.
10. Petroff, B.K., K.F. Roby, X. Gao, D.S. Son, S. Williams, D. Johnson, K.K. Rozman and P.F.
Terranova. 2001. Mechanisms controlling ovulation and their implications for the anovulatory
effects of polychlorinated dibenzo-p-dioxins (PCDDs). Toxicology 158:91-107.
11. Petroff, B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2001. The effects of 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) on weight gain and hepatic ethoxyresorufin-o-deethylase
(EROD) induction vary with ovarian hormonal status in an immature gonadotropin-primed rat
model. Reproductive Toxicology 15:269-274.
12. Petroff, B.K., X. Gao X., K. Ohshima, F. Shi, D.S. Son, K. F. Roby, K. K. Rozman, K. Taya and P.F.
Terranova. 2002. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum inhibin
concentrations and inhibin immunostaining during follicular development in female SpragueDawley rats. Reprod Toxicol 16:97-105.
13. Shi, F, B.K. Petroff, C.B. Herath, M. Ozawa, G. Watanabe and K. Taya. 2002. Serous cysts are a
Curriculum Vitae
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benign component of the cyclic ovary in the guinea pig with an incidence dependent upon
inhibin bioactivity. J Vet Res 64:129-135.
Gao, X, B.K. Petroff, O. Oluola, G. Georg, P.F. Terranova and K.K. Rozman. 2002. Endocrine
disruption by indole-3-carbinol and tamoxifen – blockage of ovulation. Toxicol Appl Pharmacol
183:179-188.
Petroff, B.K, C.R. Croutch, D.M. Hunter, M. Weirman and X. Gao. 2003. 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) disrupts gonadotropin secretion through an estradiol and
pentobarbital-sensitive mechanism but does not alter GnRH secretion by immortalized GnRH
neurons in vitro. Biol Reprod 68:2100-2106.
Petroff, B.K. and K. Mizinga. 2003. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the
pharmacokinetics of ovarian steroids in the female Sprague Dawley rat. Reprod Biol, 3:131-141.
Valdez, K.E. and Petroff, B.K. 2004. Potential roles of the aryl hydrocarbon receptor in female
reproductive senescence. Reprod Biol, 4:243-258.
Petroff , B.K. and K.M. Wegner. 2005. Preovulatory tranquilization with xylazine does not alter
the timing or hormonal profiles of ovulation in the mare. J Equine Sci, 16:67-72.
Franczak, A., Nynca, A., Valdez, K.E., Mizinga, K.M., Petroff, B.K. 2005. Effects of acute and
chronic exposure to the aryl hydrocarbon receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin
on the transition to reproductive senescence in female Sprague Dawley rats. Biol Reprod,
74:125-130.
Franczak, A., Kurowicka, B., Oponowicz, A., Petroff, B.K., and G. Kotwica. 2006. The effect of
progesterone on oxytocin-stimulated intracellular mobilization of Ca2+ and prostaglandin E2
and F2alpha from myometrial cells. Prost Lipid Mediators, 31:37-44.
Franczak, A., G. Kotwica, B. Kurowicka, A. Oponowicz, I. Woclowek-Potocka and B.K. Petroff.
2006. Effect of oxytocin on prostaglandin F2a and E2 synthesis and secretion by myometrium
during luteolysis and early pregnancy. Theriogenology, 66:1049-1056.
Petroff, B.K., Clark, J.L., Metheny, T., Xue, Q., Kimler, B.F. and C.J. Fabian. 2006. Optimization of
estrogen receptor analysis by immunocytochemistry in random periareolar fine needle
aspirates of benign breast tissue processed using thin layer preparation technology. Appl
Immunohist Mol Morph, 14:360-164.
Petroff, B.K., Phillips, T.L., Kimler, B.F. and C.J. Fabian. 2006. Detection of biomarker gene
expression by real-time polymerase chain reaction using amplified ribonucleic acids from
formalin-fixed random periareolar fine needle aspirates of human breast tissue. Anal Quant
Cytol Histol, 28:297-302.
Ting, A., B.F. Kimler, C.J. Fabian and B.K. Petroff. 2007. Development of a preclinical model of
simultaneous breast and ovarian cancer progression. Carcinogenesis, 28:130-135.
Shi Z.Q., Valdez, K.E., Ting, A., Gum, S., Franczak, A. and B.K. Petroff. 2007. Chronic AhR ligand
exposure accelerates reproductive aging via ovarian endocrine disruption at environmentally
relevant exposures. Biol Reprod, 76:198-202.
Fabian, C.J., B.F. Kimler, C.M. Zalles, Q.J. Khan, M.S. Mayo, T.A. Phillips, M. Simonsen, T. Metheny
and B.K. Petroff. 2007. Reduction in proliferation with six months of letrozole in women on
hormone replacement therapy. Breast Cancer Res Treat, 106:75-84.
Hutt, K., Shi, Z., Albertini, D.F. and B.K. Petroff. 2008 Aberrant aryl hydrocarbon receptor
activation disrupts morphogenesis of the rat conceptus. BMC Dev Biol, 8:1-12.
Ting, A., Fabian, C.J., Kimler, B.F. and B.K. Petroff. 2008 Tamoxifen prevent preneoplastic
changes of the breast but the ovary in a rat model of simultaneous breast and ovarian cancer.
Cancer Prevention Research, 1:546-553.
Valdez, K., Shi, Z., Ting, A. and B.K. Petroff. 2009 Gene expression profile accompanying
premature reproductive aging with chonic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Reprod. Toxicol., 28:32-37.
Nynca, A., Jablonska, O., Slomczynska, M., Petroff, B.K., Ciereszko, R.E. 2009 Effects of
phytoestrogen daidzein and estradiol on steroidogenesis and expression of estrogen receptors
in porcine luteinized granulosa cells from large follicles. J Physiol Pharmacol 50:95-105.
Curriculum Vitae
Petroff, Brian K.
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31. Hutt, K., Shi, X., Petroff, B.K. and Albertini, D.F. 2009 The environmental toxicant 2,3,7,8tetrachlorodibenzo-p-dioxin alters the establishment and maintenance of cell polarity in the
early embryo. Biol Reprod, 82:914-920.
32. Jablonska, O., Shi, Z., Valdez, K.E., Ting, A. and B.K. Petroff. 2010. Temporal and anatomical
sensitivities to the aryl hydrocarbon receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin
leading to premature acyclicity with age in rats. Int. J. Androl., 33:405-412.
33. Fabian, C.J., Kimler, B.F., Zalles, C.M., Klemp, J.R., Petroff, B.K., Khan, Q., Sharma, P., Setchell, K.,
Zhao, X., Phillips, T., Metheny, T., Hughes, J., Ye, H., Johnson, K. 2010. Reduction in Ki-67 in
Benign Breast Tissue of High Risk Women with the Lignan Secoisolariciresinol Diglycoside
(SDG). Cancer Prev Res, 3:1342-1350.
34. Ting, A. and Petroff, B.K. 2010. Tamoxifen rescues ovarian follicles from multiple classes of
ovarian toxicants including cancer chemotherapy. J. Assist. Reprod. Genet., 27:519-525.
35. Kim SS, Lee JR, Jee BY, Suh CS, Kim SH, Ting A, Petroff BK. 2010. Use of hormonal protection for
chemotherapy-induced gonadotoxicity. Clin. Obstet. Gynecol., 53:740-752.
36. Jablonska, O., Piasecka, J., Petroff, B.K. et. al. 2011. The effect of 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) exposure in vitro on the pituitary-ovarian axis in pigs. Theriogenology, 76:921932.
37. Petroff, B.K., Piasecka, J., Brown, S., Albertini, D.F. 2011 Dioxin disrupts fertilization and early
development of the rat embryo in vitro. Reprod Toxicol, 32:286-292.
38. Jasti, S., Warren, B., McGinnis, L.K., Kinsey, W.H., Petroff, B.K./M.G. (shared senior authorship)
2012. The autoimmune regulator (AIRE) prevents premature reproductive senescence in
female mice. Biol Reprod, in press. Published on January 4, 2012 as
DOI:10.1095/biolreprod.111.097501. Editorial: Cushman, R.A.
doi:10.1095/biolreprod.112.099242.
39. Franczak, A., Zmijewska, A., Kurowicka, B., Wojciechowicz, B., Petroff, B.K., and G. Kotwica.
2012. The effect of tumor necrosis factor alpha, interleukin 1 beta and interleukin 6 on
endometrial prostaglandin synthesis, metabolism and release in early-pregnant pigs.
Theriogenology, 77:155-165.
40. Petroff, B.K. 2012. A case for combined breast and ovarian cancer prevention. Med. Hypoth,
79:549-551.
41. Petroff, B.K., Phillips, T., Kimler, B.F., Fabian, C.J. 2013 Comparison of RNA endpoints in fixed
vs. frozen benign human breast tissue harvested by ductal lavage or random periareolar fine
needle aspiration (RPFNA). Reprod Biol, 13:75-81.
42. Fabian, C.J., Kimler, B.F., Donnelly, J.E., Sullivan, D.K., Klemp, J.R., Petroff, B.K., et. al. 2013.
Favorable modulation of benign breast tissue and serum risk biomarkers is associated with
>10% weight loss in postmenopausal women. Breast Canc Res Trt 142: 119-132.
Manuscripts submitted by not yet accepted: Provide names of all authors (applicant’s name in
BOLD), title, journal.
1. Piasecka, J., Delman, D., Ciereszko, R.E., Petroff, B.K. 2014. Tamoxifen prevents chemotherapymediated apoptosis and follicular depletion in vitro. Biol. Reprod., accepted pending revision.
2. Delman, D., Kimler,B.F., Fabian C.J., Petroff, B.K. 2014. Effect of flaxseed lignan
secoisoresoricinol diglucoside on preneoplasia in a rat model of early simultaneous breast and
ovarian cancer progression. Canc Prev Res, submitted.
3. Stecklein, S., Jensen, R., Petroff, B.K., Kimler, B.F., Fabian, C.J., Godwin, A., Sharma, P. 2014.
BRCA1 promoter methylation in triple negative breast cancer is associated with poor response
to anthracycline-based chemotherapy. Breast Canc Res Trtmt, in review.
4. Fabian, C.J., Kimler, B.F., Petroff, B.K., Metheny, T., Phillip, T.M., Zalles, C., Klemp, J. 2014 Impact
of a validated weight loss intervention on markers of risk in premenopausal women at elevated
Curriculum Vitae
Petroff, Brian K.
P a g e | 17
risk for breast cancer. J Breast Cancer Res, submitted.
5. Warren, B., Jasti, S., Petroff, B.K., Petroff, M.G. 2014 AIRE deficiency is associated with multiple
reproductive defects leading to subfertility in mice. Reproduction, submitted.
Published Abstracts: Provide names of all authors (applicant’s name in BOLD), year, title, where
published, volume and pages. If the same work is reflected in a published abstract and a presentation or
poster, the work must only be listed once.
1. Petroff, B.K., A.M. Iacopino, E.K. Miller, X.F. Zhuang and N. Inpanbutr. 1993. Changes in uterine
calbindin-D9k expression through the bovine estrous cycle. Biol. Reprod. 43 (Suppl. 1), 221.
2. Ciereszko, R.E., Z. Guan, B.K. Petroff et. al. 1993. Arachidonic acid inhibits progesterone
production by corpora lutea of primates. Biol. Reprod. 48 (Suppl. 1), 392.
3. Ciereszko, R.E., B.K. Petroff, A.C. Ottobre and J.S. Ottobre. 1994. The effect of prolactin on
progesterone production and protein kinase C activity in porcine luteal cells collected during
the early luteal phase. Biol. Reprod. 50 (Suppl.1), 242.
4. Petroff, B.K., K. Dabrowski, R.E. Ciereszko and J.S. Ottobre. 1995. Ascorbate and
dehydroascorbate concentrations in porcine corpora lutea, follicles and ovarian stroma
collected throughout the estrous cycle and pregnancy. Biol. Reprod. (Suppl. 1), 109.
5. Ciereszko, R.E., B.K. Petroff et al., 1995. The effects of mammalian and piscine gonadotropin on
steroid production by ovaries of yellow perch (Perca flavescens): possible mechanisms of action.
Biol. Reprod. 52 (Suppl. 1), 110.
6. Petroff, B.K., K. Dabrowski et al. 1996. Prostaglandin F2a depletes luteal ascorbate by
inducing secretion of the vitamin from porcine corpora lutea. Biol. Reprod. 53 (Suppl. 1), 178.
7. Petroff, M.G., B.K. Petroff and J.L. Pate. 1998. Detection of cytokine mRNAs by reverse
transcription-polymerase chain reaction in the bovine corpus luteum. Biol. Reprod. 56(Suppl.
1), 368.
8. Terranova, P.F., X. Gao, B.K. Petroff, and K.K. Rozman. 2000. Exogenous gonadotropin releasing
hormone induces luteinizing hormone and follicle stimulating hormone surges and partially
restores ovulation in an eCG-primed immature rat model treated with 2,3,7,8tetrachlorodibenzo-p-dioxin. Toxicol. Sci. 54 (Suppl. 1), 1310.
9. Petroff B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2000. Interaction of estradiol and 2,3,7,8tetrachlorodibenzo-p-dioxin in and ovulation model: evidence for systemic and local effects.
Toxicol. Sci. 54 (Suppl. 1), 1312.
10. Petroff, B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2001. The effects of 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) on weight gain and hepatic ethoxyresorufin-o-deethylase
(EROD) induction vary with ovarian hormonal status in an immature gonadotropin-primed rat
model. Toxicol. Sci. 60 (Suppl. 1), 1198.
11. Petroff, BK, Gao X, Ohshima K, Shi F, Son D-S, Roby KF, Rozman KK, Taya K, Terranova PF.
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum inhibin concentrations and
inhibin immunostaining during follicular development in female Sprague-Dawley rats. Toxicol
Sci 2002; 66(Suppl. 1):1840.
12. Gao, X, Petroff, BK, Oluola, O, Georg, G, Terranova, PF, Rozman, KK. Endocrine disruption by
indole-3-carbinol and tamoxifen – blockage of ovulation. Toxicol Sci 2002; 66(Suppl. 1):1841
13. Fried, KW, Gao, X, Petroff, BK, Schramm, KW, Terranova, PF, Rozman KK. Effects of chlorinated
phenothiazines on ovulation in rats. Toxicol Sci 2002; 66(Suppl. 1):835
14. Petroff, BK. Prepubertal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is associated
with a premature transition to reproductive senescence in female Sprague-Dawley rats. Biol
Reprod. 2003; 69 (Suppl. 1):1045.
15. Fabian CJ, Petroff BK, Kimler BF, Clark J, Metheny T, Stecker K, Salunga R, Erlander M. 2004.
Quantitative RT-PCR to assess change in molecular expression in methanol-formalin fixed
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cytology specimens obtained by random periareolar FNA from women on Phase II breast
cancer chemoprevention trials. Cancer Epidemiol Biomarkers Prev 12:1301s, abstract 194.
Petroff BK, Gum SL, Phillips TA, Clark JS, Kimler BF, Fabian CJ. 2004 Assessment of risk
biomarker gene expression by real time reverse transcription-polymerase chain reaction using
amplified ribonucleic acids from formalin-fixed fine needle aspirates of human breast tissue: A
preliminary report. Cancer Epidemiol Biomarkers Prev 13:1921s, abstract C61.
Clark JL, Petroff BK, Metheny T, Xue Q, Kimler BF, Fabian CJ: Optimization of
immunocytochemical assessment of estrogen receptor expression in benign breast epithelial
cells obtained by random periareolar fine needle aspiration and processed using thin layer
preparation technology. Proc Am Assoc Cancer Res 46:377 (abstract 1606), 2005.
Petroff BK, Tawfik O, Gum SL, Nynca A, Valdez KE, Kimler BF, Fabian CJ. 2005. Development of
a rat model of simultaneous mammary and ovarian cancer: Expression of putative early
markers of cancer risk and progression. Proc Am Assoc Cancer Res 46:1429, (abstract 6075).
Valdez KE, Kimler BF, Fabian CJ, Petroff BK. A proteomic assessment of serum and fine needle
aspirate specimens to identify increased risk and response to breast cancer prevention
strategies. In: Program of the Kansas City Area Life Science’s Research Day, Overland Park, KS,
2005. Abstract G3.
Petroff BK, Franczak A, Valdez KE, Mizinga KM. Chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin accelerates reproductive senescence in
the female rat. In: Program of the 38th Annual Meeting of the Society for the Study of
Reproduction, Quebec City, Quebec, Canada 2005.
Petroff BK, Metheny T, Xue Q, Valdez KE, Zalles CM, Kimler BF, Jabian CJ. Repeatability and
long term stability of Ki-67 immunostaining, cell numbers and cytologic morphology in random
periareolar fine needle aspiration samples of breast tissue processed as thin layer preparations.
In: Program and Proceedings of 4th Annual AACR International Conference on Frontiers in
Cancer Prevention Research. Abstract C15.
Valdez KE, Kimler BF, Fabian CJ, Petroff BK. Optimization of Proteomic Assessment of Breast
random Periareolar fine-needle aspiration specimens from high risk women using SELDI-TOFMS. In: Program and Proceedings of 4th Annual AACR International Conference on Frontiers in
Cancer Prevention Research. Abstract B17
Valdez KE, Kimler BF, Fabian CJ, Petroff BK. Proteomic assessment of breast random
periareolar fine-needle aspiration specimens from high risk women using SELDI-TOF-MS. In:
Program and Proceedings of 5th Annual AACR International Conference on Frontiers in Cancer
Prevention Research. 2006 Abstract A22.
Ting AY, Kimler BF, Fabian CJ, Petroff BK. Tamoxifen decreases mammary but not ovarian
preneoplasia in a preclinical model of simultaneous breast and ovarian cancer progression. In:
Program and Proceedings of 5th Annual AACR International Conference on Frontiers in Cancer
Prevention Research 2006. Abstract A68.
Petroff BK, Fabian CJ, Phillips. 2007. Analysis of biomarker gene expression by LCM and real
time PCR in breast epithelial cells harvested by RPFNA and ductal lavage. Proceedings of the 4th
meeting of Dr. Susan Love Research Foundation.
Petroff BK. 2007. Chronic activation of the aryl hydrocarbon receptor pathway and
reproductive senescence. Annual meeting of the Society of Toxicology, March 25-29, 2007. The
Toxicologist, 96 (suppl. 1), abstract 692.
Phillips TA, Petroff BK, Fabian CJ. 2007. Comparison of protocols for the isolation and
amplification of biomarker RNA from small numbers of formalin-fixed human breast cancer
cells following thin layer preparation and laser capture microdissection. Annual meeting of the
AACR, April 14-18; Los Angeles, CA. AACR Proceedings 48: abstract 1668.
Hutt K, Shi Z, Albertini DF, Petroff BK. 2007. Early embryonic defects in chromosome and
blastomere allocation are induced by the aryl hydrocarbon receptor specific environmental
teratogen, 2,3,7,8-tetrachlorodibenzo-p-dioxin. Annual meeting of the Society for the Study of
Reproduction, July 21-25, 2007, San Antonio, TX. Biol Reprod (Suppl 1), abstract 154.
Curriculum Vitae
Petroff, Brian K.
P a g e | 19
29. Ting A, Shi Z, Valdez KE, Petroff BK. 2007. Use of a syngeneic orthotopic ovary transplantation
model to assess the relative impact of ovarian vs. extraovarian and developmental vs. adult
activation of the aryl hydrocarbon receptor pathway. Annual meeting of the Society for the
Study of Reproduction, July 21-25, 2007, San Antonio, TX. Biol Reprod (Suppl 1), abstract 542.
30. Valdez KE, Shi Z, Ting A, Petroff BK. 2007. Effect of chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin in female rats on ovarian gene expression.
Annual meeting of the Society for the Study of Reproduction, July 21-25, 2007, San Antonio, TX.
Biol Reprod (Suppl 1), abstract 542.
31. Petroff BK, Fabian CJ, Phillips T. 2007. Isolation and amplification of biomarker RNA from small
numbers of archived formalin-fixed human breast epithelial cells harvested by random
periareolar fine needle aspiration or ductal lavage followed by laser capture microdissection.
Annual meeting of the Society for the Study of Reproduction, July 21-25, 2007, San Antonio, TX.
Biol Reprod (Suppl 1), abstract 596.
32. Luense LJ, Ting A, and Petroff BK. 2008 A meta-analysis to identify shared biomarker genes
and prevention targets for human breast and ovarian cancer. Annual American Association of
Cancer Research Meeting. abstract 7050.
33. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. 2008 Expression of putative stem cell marker
genes in breast epithelial cells from normal and neoplastic breast tissue as measured by realtime PCR. In: 2008 Proceedings of the American Association for Cancer Research. Abstract #
2018.
34. Valdez KE, Brown SB, Albertini D, Petroff BK. 2009 The aryl hydrocarbon receptor agonist
2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) inhibits early embryonic development following in
vitro fertilization in female Sprague Dawley rats. In: Program of the 41th Annual Meeting of the
Society for the Study of Reproduction, Kona, HI. Abstract # 134.
35. Ting AY and Petroff BK. 2009. Tamoxifen rescues the ovarian follicular reserve following
exposure to multiple classes of ovotoxic drugs including cancer chemotherapy. [abstract] In:
Proceedings of the 100th Annual Meeting of the American Association for Cancer Research;
37:877
36. Petroff BK. 2010 Effect of chronic dioxin exposure on reproduction in the female rat. Annual
Meeting of the Society for the Study of Reproduction. Aug 1-3, Milwaukee, WI. Biol Reprod
(Suppl 1), abstract 209.
37. Warren BD, Jasti S, Petroff BK, Petroff MG. 2012 Autoimmune deficiency is associated with
embryonic loss and generation of autoantibodies against the uterus, placenta and embryo.
Annual Meeting of the Society for the Study of Reproduction, Aug 5-6, State College, PA.
38. Kimler BF, Metheny T, Phillips T, Zalles C, Petroff BK, Fabian CJ. 2012 Reduction in Ki-67 in
benign breast tissue of high risk premenopausal women with the SERM acolbiphene. Annual
Meeting of the American Society of Clinical Oncology, June 1-5, 2012, Chicago, IL.
39. Petroff BK. 2012 Effects of tamoxifen on markers of follicular health in rats treated with
cancer chemotherapy. Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
40. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice..
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
41. Li S, Hidaka BH, Harvey KE, Sullivan DK, Klemp JR, Carlson SE, Petroff BK, Kimler BF, Fabian CJ.
2012 Breast cancer risk biomarkers are associated with dietary intake and tissue content of n-3
polyunsaturated fatty acids. Annual Meeting of the International Society for the Study of Fatty
Acid Lipids, May 26-30, 2012, Vancouver, CA.
42. Delman, D., Fabian, C.J., Kimler, B.F., Petroff B.K. 2013. Flaxseed lignan prevents early changes
of mammary carcinogenesis in rats at high risk for breast and ovarian cancer. Annual Meeting of
the American Association for Cancer Research, April 6-10, 2013, Washington D.C.
Curriculum Vitae
Petroff, Brian K.
P a g e | 20
43. Delman, D. and Petroff B.K. Mechanisms of follicular rescue by tamoxifen in
44.
45.
46.
47.
cyclophosphamide-treated rat ovaries. 2013. Annual Meeting of the Society for the Study of
Reproduction. July 25, 2013.
Fabian C, Kimler B, Petroff B.K., Zalles C, Hursting S, Nydegger J, Phillips T, Metheny T: Lack of
modulation of serum and breast biomarkers by high dose Vitamin D supplementation in women
at high risk for breast cancer. American Society for Preventive Oncology Annual Meeting; 10-12
Mar 2013; Abst 25.
Fabian CF, Kimler BF, Petroff BK, Zalles CM, Metheny T, Box JA, Nydegger JL, Phillips TA, Hidaka
BH, Carlson SE, deGraffenried LA, Hursting SD: High dose omega-3 fatty acid (FA)
supplementation modulates breast tissue biomarkers in pre-menopausal women at high risk
for development of breast cancer. J Clin Oncol 31, 2013 (suppl; abstr 1515).
Fabian CJ, Kimler BF, Petroff BK, Zalles CM, Metheny T, Nydegger JL, Box JA, Phillips TA, Hidaka
BH, Carlson SE, deGraffenried LA, Hursting SD: High dose omega-3 fatty acid supplementation
modulates breast tissue biomarkers in post-menopausal women at high risk for development of
breast cancer. 2013 San Antonio Breast Cancer Symposium. 10-14 Dec 2013. P4-10-01.
Fabian CJ, Kimler BF, Nydegger JL, Metheny T, Zalles CM, Petroff BK, Yeh H, Alvarado MD. Initial
report of a randomized trial of letrozole in high risk women taking hormone replacement
therapy. 2014 San Antonio Breast Cancer Symposium.
3. Presentations and Posters:
Presentations and posters must be number in chronological order, oldest first, most recent last.
Oral paper presentation:
Provide names of all authors (applicant’s name in BOLD), title, sponsoring organization, and location
and date of presentation.
Scientific papers presented at national and international meetings:
1. Ciereszko, R.E., Z. Guan, B.K. Petroff et. al. 1993. Arachidonic acid inhibits progesterone
production by corpora lutea of primates. Biol. Reprod. 48 (Suppl. 1), 392.
2. Petroff, B.K., K. Dabrowski, R.E. Ciereszko and J.S. Ottobre. 1995. Ascorbate and
dehydroascorbate concentrations in porcine corpora lutea, follicles and ovarian stroma
collected throughout the estrous cycle and pregnancy. Biol. Reprod. (Suppl. 1), 109.
3. Petroff, B.K., K. Dabrowski et al. 1996. Prostaglandin F2a depletes luteal ascorbate by
inducing secretion of the vitamin from porcine corpora lutea. Biol. Reprod. 53 (Suppl. 1), 178.
4. Petroff, M.G., B.K. Petroff and J.L. Pate. 1998. Detection of cytokine mRNAs by reverse
transcription-polymerase chain reaction in the bovine corpus luteum. Biol. Reprod. 56(Suppl.
1), 368.
5. Terranova, P.F., X. Gao, B.K. Petroff, and K.K. Rozman. 2000. Exogenous gonadotropin releasing
hormone induces luteinizing hormone and follicle stimulating hormone surges and partially
restores ovulation in an eCG-primed immature rat model treated with 2,3,7,8tetrachlorodibenzo-p-dioxin. Toxicol. Sci. 54 (Suppl. 1), 1310.
6. Petroff, BK, Gao X, Ohshima K, Shi F, Son D-S, Roby KF, Rozman KK, Taya K, Terranova PF.
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum inhibin concentrations and
inhibin immunostaining during follicular development in female Sprague-Dawley rats. Toxicol
Sci 2002; 66(Suppl. 1):1840.
Curriculum Vitae
Petroff, Brian K.
P a g e | 21
7. Petroff BK, Tawfik O, Gum SL, Nynca A, Valdez KE, Kimler BF, Fabian CJ. 2005 Development of a
rat model of simultaneous mammary and ovarian cancer: expression of putative early markers
of cancer risk and progression. Proc Am Assoc Cancer Res 46:1429 (abstract 6075).
8. Petroff, BK. Prepubertal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is associated
with a premature transition to reproductive senescence in female Sprague-Dawley rats. Biol
Reprod. 2003; 69 (Suppl. 1):1045.
9. Petroff BK, Franczak A, Valdez KE, Mizinga KM. 2005 Chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin accelerates reproductive senescence in
the female rat. Society for the Study of Reproduction Annual Meeting, W231.
10. Ting AY, Xue Q, Tawfik O, Kimler BF, Fabian CJ, Petroff BK Cancer biomarker expression in a
preclinical model for chemoprevention of breast and ovarian cancer. April 2006, Washington,
DC. Proc Amer Assoc Cancer Res 2006;47:697
11. Ting AY, Kimler BF, Fabian CJ, Petroff BK. Tamoxifen decreases mammary but not ovarian
preneoplasia in a preclinical model of simultaneous breast and ovarian cancer progression. In:
Program and Proceedings of 5th Annual AACR International Conference on Frontiers in Cancer
Prevention Research 2006. Abstract A68.
12. Petroff BK, Fabian CJ, Phillips. 2007. Analysis of biomarker gene expression by LCM and real
time PCR in breast epithelial cells harvested by RPFNA and ductal lavage. Proceedings of the 4th
meeting of Dr. Susan Love Research Foundation.
13. Petroff BK. 2007. Chronic activation of the aryl hydrocarbon receptor pathway and
reproductive senescence. Annual meeting of the Society of Toxicology, March 25-29, 2007. The
Toxicologist, 96 (suppl. 1), abstract 692.
14. Petroff BK. 2010 Effect of chronic dioxin exposure on reproduction in the female rat. Annual
Meeting of the Society for the Study of Reproduction. Aug 1-3, Milwaukee, WI. Biol Reprod
(Suppl 1), abstract 209.
15. Ting AY and Petroff BK. 2009. Tamoxifen rescues the ovarian follicular reserve following
exposure to multiple classes of ovotoxic drugs including cancer chemotherapy. [abstract] In:
Proceedings of the 100th Annual Meeting of the American Association for Cancer Research;
37:877
16. Valdez KE, Brown SB, Albertini D, Petroff BK. 2009 The aryl hydrocarbon receptor agonist
2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) inhibits early embryonic development following in
vitro fertilization in female Sprague Dawley rats. In: Program of the 41th Annual Meeting of the
Society for the Study of Reproduction, Kona, HI. Abstract # 134.
17. Ting AY and Petroff BK. 2009. Tamoxifen rescues the ovarian follicular reserve following
exposure to multiple classes of ovotoxic drugs including cancer chemotherapy. [abstract] In:
Proceedings of the 100th Annual Meeting of the American Association for Cancer Research;
37:877.
18. Kimler BF, Metheny T, Phillips T, Zalles C, Petroff BK, Fabian CJ. 2012 Reduction in Ki-67 in
benign breast tissue of high risk premenopausal women with the SERM acolbiphene. Annual
Meeting of the American Society of Clinical Oncology, June 1-5, 2012, Chicago, IL
19. Warren BD, Jasti S, Petroff BK, Petroff MG. 2012 Autoimmune deficiency is associated with
embryonic loss and generation of autoantibodies against the uterus, placenta and embryo.
Annual Meeting of the Society for the Study of Reproduction, Aug 5-6, State College, PA.
20. Delman, D. and Petroff, B.K. Mechanisms of follicular rescue by tamoxifen in
cyclophosphamide-treated rat ovaries. 2013. Annual Meeting of the Society for the Study of
Reproduction. July 25, 2013.
21. Fabian C, Kimler B, Petroff B.K., Zalles C, Hursting S, Nydegger J, Phillips T, Metheny T: Lack of
modulation of serum and breast biomarkers by high dose Vitamin D supplementation in women
at high risk for breast cancer. American Society for Preventive Oncology Annual Meeting; 10-12
Mar 2013; Abst 25.
Curriculum Vitae
Petroff, Brian K.
P a g e | 22
Scientific papers presented at local and regional meetings:
1. Ting AY, Valdez KE, Xue Q, Kimler BF, Fabian CJ, Petroff BK Development of a rat model of
simultaneous mammary and ovarian cancer. October 2005, The Greenwald Symposium, Kansas
City, KS.
2. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. A proteomic assessment of serum and fine needle
aspirate specimens to identify increased risk and response to breast cancer prevention
strategies. In: Program of the Kansas City Area Life Science’s Research Day, Overland Park, KS,
2005. Abstract G3.
3. Ting A, Petroff BK. A preclinical model of simultaneous breast and ovarian cancer. The 3rd
Annual Gilbert S. Greenwald Symposium on Reproduction (platform presentation), October,
2006, Kansas City, MO.
4. Petroff, BK 2008 Stem cells and breast cancer prevention. Annual Research Meeting of the KU
Cancer Center, Lawrence, KS.
5. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice.
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
6. Ting AY and Petroff BK. Protective Effect of Tamoxifen Against Ovarian Follicle Loss Caused by
Multiple Ovotoxic Agents. April 2008. Department of Molecular & Integrative Physiology,
University of Kansas Medical Center, Kansas City, KS.
7. Ting AY and Petroff BK. Tamoxifen rescues the ovarian follicular reserve following exposure to
multiple classes of ovotoxic drugs including cancer chemotherapeutics. 2008 Student Research
Forum, University of Kansas Medical Center.
8. Petroff BK. A novel approach for fertility preservation in cancer patients. Internal Medicine
Grand Rounds Translational Medicine conference, University of Kansas Medical Center,
4/09/08.
9. Petroff, BK 2008 Stem cells and breast cancer prevention. Annual Research Meeting of the KU
Cancer Center, Lawrence, KS.
10. Petroff BK. 2012 Effects of tamoxifen on markers of follicular health in rats treated with
cancer chemotherapy. Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
11. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice. .
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
12. Delman, D. and Petroff, B.K. Mechanisms of follicular rescue by tamoxifen in
cyclophosphamide-treated rat ovaries. 2013. Annual Greenwald Symposium, Oct 11-12, 2013.
Kansas City, MO.
Poster Presentations:
Provide names of all authors (applicant’s name in BOLD), title, sponsoring organization, extent of peerreview, and location and date of presentation.
Poster presentation at national and international meetings:
1. Petroff, B.K., A.M. Iacopino, E.K. Miller, X.F. Zhuang and N. Inpanbutr. 1993. Changes in uterine
calbindin-D9k expression through the bovine estrous cycle. Biol. Reprod. 43 (Suppl. 1), 221.
2. Ciereszko, R.E., Z. Guan, B.K. Petroff et. al. 1993. Arachidonic acid inhibits progesterone
production by corpora lutea of primates. Biol. Reprod. 48 (Suppl. 1), 392.
3. Ciereszko, R.E., B.K. Petroff, A.C. Ottobre and J.S. Ottobre. 1994. The effect of prolactin on
progesterone production and protein kinase C activity in porcine luteal cells collected during
the early luteal phase. Biol. Reprod. 50 (Suppl.1), 242.
Curriculum Vitae
Petroff, Brian K.
P a g e | 23
4. Petroff, B.K., K. Dabrowski, R.E. Ciereszko and J.S. Ottobre. 1995. Ascorbate and
dehydroascorbate concentrations in porcine corpora lutea, follicles and ovarian stroma
collected throughout the estrous cycle and pregnancy. Biol. Reprod. (Suppl. 1), 109.
5. Ciereszko, R.E., B.K. Petroff et al., 1995. The effects of gonadotropin on steroid production by
ovaries of yellow perch (Perca flavescens): possible mechanisms of action. Biol. Reprod. 52
(Suppl. 1), 110.
6. Petroff, B.K., K. Dabrowski et al. 1996. Prostaglandin F2a depletes luteal ascorbate by
inducing secretion of the vitamin from porcine corpora lutea. Biol. Reprod. 53 (Suppl. 1), 178.
7. Petroff, M.G., B.K. Petroff and J.L. Pate. 1998. Detection of cytokine mRNAs by reverse
transcription-polymerase chain reaction in the bovine corpus luteum. Biol. Reprod. 56(Suppl.
1), 368.
8. Terranova, P.F., X. Gao, B.K. Petroff, and K.K. Rozman. 2000. Exogenous gonadotropin releasing
hormone induces luteinizing hormone and follicle stimulating hormone surges and partially
restores ovulation in an eCG-primed immature rat model treated with 2,3,7,8tetrachlorodibenzo-p-dioxin. Toxicol. Sci. 54 (Suppl. 1), 1310.
9. Petroff B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2000. Interaction of estradiol and 2,3,7,8tetrachlorodibenzo-p-dioxin in and ovulation model: evidence for systemic and local effects.
Toxicol. Sci. 54 (Suppl. 1), 1312.
10. Petroff, B.K., X. Gao, K.K. Rozman and P.F. Terranova. 2001. The effects of 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) on weight gain and hepatic ethoxyresorufin-o-deethylase
(EROD) induction vary with ovarian hormonal status in a gonadotropin-primed rat model.
Toxicol. Sci. 60 (Suppl. 1), 1198.
11. Petroff, BK, Gao X, Ohshima K, Shi F, Son D-S, Roby KF, Rozman KK, Taya K, Terranova PF.
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum inhibin concentrations and
inhibin immunostaining during follicular development in female Sprague-Dawley rats. Toxicol
Sci 2002; 66(Suppl. 1):1840.
12. Gao, X, Petroff, BK, Oluola, O, Georg, G, Terranova, PF, Rozman, KK. Endocrine disruption by
indole-3-carbinol and tamoxifen – blockage of ovulation. Toxicol Sci 2002; 66(Suppl. 1):1841
13. Fried, KW, Gao, X, Petroff, BK, Schramm, KW, Terranova, PF, Rozman KK. Effects of chlorinated
phenothiazines on ovulation in rats. Toxicol Sci 2002; 66(Suppl. 1):835
14. Petroff, BK. Prepubertal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is associated
with a premature transition to reproductive senescence in female Sprague-Dawley rats. Biol
Reprod. 2003; 69 (Suppl. 1):1045.
15. Fabian CJ, Petroff BK, Kimler BF, Clark J, Metheny T, Stecker K, Salunga R, Erlander M. 2004.
Quantitative RT-PCR to assess change in molecular expression in methanol-formalin fixed
cytology specimens obtained by random periareolar FNA from women on Phase II breast
cancer chemoprevention trials. AACR A194.
16. Clark JL, Petroff BK, Metheny T, Xue Q, Zalles CM, Hall M, Fabian CJ. 2005 Optimization of
immunocytochemical assessment of estrogen receptor expression in benign breast epithelial
cells obtained by random periareolar fine needle aspiration and processed using thin layer
preparation technology. . Proc Am Assoc Cancer Res 46:377 (abstract 1606).
17. Petroff BK, Tawfik O, Gum SL, Nynca A, Valdez KE, Kimler BF, Fabian CJ. 2005 Development of a
rat model of simultaneous mammary and ovarian cancer: expression of putative early markers
of cancer risk and progression. Proc Am Assoc Cancer Res 46:1429 (abstract 6075).
18. Petroff BK, Franczak A, Valdez KE, Mizinga KM. 2005 Chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin accelerates reproductive senescence in
the female rat. Society for the Study of Reproduction Annual Meeting, W231.
19. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. A proteomic assessment of serum and fine needle
aspirate specimens to identify increased risk and response to breast cancer prevention
strategies. In: Program of the Kansas City Area Life Science’s Research Day, Overland Park, KS,
2005. Abstract G3.
Curriculum Vitae
Petroff, Brian K.
P a g e | 24
20. Petroff BK, Franczak A, Valdez KE, Mizinga KM. Chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin accelerates reproductive senescence in
the female rat. In: Program of the 38th Annual Meeting of the Society for the Study of
Reproduction, Quebec City, Quebec, Canada 2005.
21. Petroff BK, Methany T, Xue Q, Valdez KE, Zalles CM, Kimler BF, Fabian CJ. Repeatability and
long term stability of Ki-67 immunostaining, cell numbers and cytologic morphology in random
periareolar fine needle aspiration samples of breast tissue processed as thin layer preparations.
In: Program and Proceedings of 4th Annual AACR International Conference on Frontiers in
Cancer Prevention Research. 2006. Abstract C15.
22. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. Optimization of Proteomic Assessment of Breast
random Periareolar fine-needle aspiration specimens from high risk women using SELDI-TOFMS. In: Program and Proceedings of 4th Annual AACR International Conference on Frontiers in
Cancer Prevention Research. Abstract B17
23. Ting AY, Valdez KE, Xue Q, Kimler BF, Fabian CJ, Petroff BK Development of a rat model of
simultaneous mammary and ovarian cancer. October 2005, The Greenwald Symposium, Kansas
City, KS.
24. Ting AY, Xue Q, Tawfik O, Kimler BF, Fabian CJ, Petroff BK Cancer biomarker expression in a
preclinical model for chemoprevention of breast and ovarian cancer. April 2006, Washington,
DC. Proc Amer Assoc Cancer Res 2006;47:697
25. Kraszewska O, Nynca A, Petroff BK, Wasowska B, Pawlowska A, Ciereszko RE. Involvement of
environmental estrogens in the regulation of granulose cell function in pigs. 15th UNESCO/PAS
Symposium: Molecular and physiological aspects of regulatory processes of the organism. June
2006, Krakow, Poland.
26. Ting A, Petroff BK. A preclinical model of simultaneous breast and ovarian cancer. The 3rd
Annual Gilbert S. Greenwald Symposium on Reproduction (platform presentation), October,
2006, Kansas City, MO.
27. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. Proteomic assessment of breast random
periareolar fine-needle aspiration specimens from high risk women using SELDI-TOF-MS. 5th
Annual AACR International Conference on Frontiers in Cancer Prevention Research, November
2006, Boston, MA.
28. Ting AY, Kimler BF, Fabian CJ, Petroff BK. Tamoxifen decreases mammary but not ovarian
preneoplasia in a preclinical model of simultaneous breast and ovarian cancer progression. In:
Program and Proceedings of 5th Annual AACR International Conference on Frontiers in Cancer
Prevention Research 2006. Abstract A68.
29. Petroff BK, Fabian CJ, Phillips. 2007. Analysis of biomarker gene expression by LCM and real
time PCR in breast epithelial cells harvested by RPFNA and ductal lavage. Proceedings of the 4th
meeting of Dr. Susan Love Research Foundation.
30. Petroff BK. 2007. Chronic activation of the aryl hydrocarbon receptor pathway and
reproductive senescence. Annual meeting of the Society of Toxicology, March 25-29, 2007. The
Toxicologist, 96 (suppl. 1), abstract 692.
31. Phillips TA, Petroff BK, Fabian CJ. 2007. Comparison of protocols for the isolation and
amplification of biomarker RNA from small numbers of formalin-fixed human breast cancer
cells following thin layer preparation and laser capture microdissection. Annual meeting of the
AACR, April 14-18; Los Angeles, CA. AACR Proceedings 48: abstract 1668.
32. Hutt K, Shi Z, Albertini DF, Petroff BK. Early embryonic defects in chromosome and blastomere
allocation are induced by the aryl hydrocarbon receptor specific environmental teratogen
2,3,7,8-tetrachlorodibenzo-p-dixoin. 40th Annual Meeting of the Society for the Study of
Reproduction, July 21-25, 2007; San Antonio, TX. Abstract 154.
33. Ting A, Shi Z, Valdez KE, Petroff BK. Use of a syngeneic orthotopic ovary transplanatation
model to assess the relative impact of ovarian vs. extraovarian and developmental vs. adult
activation of the aryl hydrocarbon receptor pathway. 40th Annual Meeting of the Society for the
Study of Reproduction, July 21-25, 2007; San Antonio, TX. Abstract 542.
Curriculum Vitae
Petroff, Brian K.
P a g e | 25
34. Petroff BK, Fabian CJ, Phillips TS. Isolation and amplification of biomarker RNA from small
numbers of archived formalin-fixed human breast epithelial cells harvesxted by random
periareolar fine needle aspiration or ductal lavage followed by laser capture microdissection.
40th Annual Meeting of the Society for the Study of Reproduction. July 21-25; San Antonio, TX.
Abstract 596.
35. Valdez KE, Shi Z, Ting A, Petroff BK. Effect of chronic exposure to the aryl hydrocarbon
receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin on ovarian gene expression in female rats.
40th Annual Meeting of the Society for the Study of Reproduction. July 21-25, 2007; San
Antonio, TX. Abstract 543.
36. Ting AY and Petroff BK. Protective Effect of Tamoxifen Against Ovarian Follicle Loss Caused by
Multiple Ovotoxic Agents. April 2008. Department of Molecular & Integrative Physiology,
University of Kansas Medical Center, Kansas City, KS.
37. Ting AY and Petroff BK. Tamoxifen rescues the ovarian follicular reserve following exposure to
multiple classes of ovotoxic drugs including cancer chemotherapeutics. 2008 Student Research
Forum, University of Kansas Medical Center.
38. Petroff BK. A novel approach for fertility preservation in cancer patients. Internal Medicine
Grand Rounds Translational Medicine conference, University of Kansas Medical Center,
4/09/08.
39. Luense LJ, Ting A, and Petroff BK. 2008 A meta-analysis to identify shared biomarker genes
and prevention targets for human breast and ovarian cancer. Annual American Association of
Cancer Research Meeting. abstract 7050.
40. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. 2008 Expression of putative stem cell marker
genes in breast epithelial cells from normal and neoplastic breast tissue as measured by realtime PCR. In: 2008 Proceedings of the American Association for Cancer Research. Abstract #
2018.
41. Valdez KE, Brown SB, Albertini D, Petroff BK. 2008 The aryl hydrocarbon receptor agonist
2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) inhibits early embryonic development following in
vitro fertilization in female Sprague Dawley rats. In: Program of the 41th Annual Meeting of the
Society for the Study of Reproduction, Kona, HI. Abstract # 134.
42. Petroff, BK 2008 Stem cells and breast cancer prevention. Annual Research Meeting of the KU
Cancer Center, Lawrence, KS.
43. Ting AY and Petroff BK. 2009. Tamoxifen rescues the ovarian follicular reserve following
exposure to multiple classes of ovotoxic drugs including cancer chemotherapy. [abstract] In:
Proceedings of the 100th Annual Meeting of the American Association for Cancer Research;
37:877.
44. Valdez KE, Brown SB, Albertini D, Petroff BK. 2009 The aryl hydrocarbon receptor agonist
2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) inhibits early embryonic development following in
vitro fertilization in female Sprague Dawley rats. In: Program of the 41th Annual Meeting of the
Society for the Study of Reproduction, Kona, HI. Abstract # 134.
45. Ting AY and Petroff BK. 2009. Tamoxifen rescues the ovarian follicular reserve following
exposure to multiple classes of ovotoxic drugs including cancer chemotherapy. [abstract] In:
Proceedings of the 100th Annual Meeting of the American Association for Cancer Research;
37:877
46. Petroff BK. 2010 Effect of chronic dioxin exposure on reproduction in the female rat. Annual
Meeting of the Society for the Study of Reproduction. Aug 1-3, Milwaukee, WI. Biol Reprod
(Suppl 1), abstract 209.
47. Sharma, B. F. Kimler, Y. A. Park, S. R. Stecklein, Q. J. Khan, B. K. Petroff, O. W. Tawfik, R. A.
Jensen; University of Kansas Cancer Center, Kansas City, KS. Association of BRCA1 promoter
methylation in triple-negative breast cancer (TNBC) with resistance to standard anthracylinebased adjuvant chemotherapy. 2011 ASCO Annual Meeting. J Clin Oncol 29: 2011 (suppl; abstr
1123).
Curriculum Vitae
Petroff, Brian K.
P a g e | 26
48. Warren BD, Jasti S, Petroff BK, Petroff MG. 2012 Autoimmune deficiency is associated with
embryonic loss and generation of autoantibodies against the uterus, placenta and embryo.
Annual Meeting of the Society for the Study of Reproduction, Aug 5-6, State College, PA.
49. Kimler BF, Metheny T, Phillips T, Zalles C, Petroff BK, Fabian CJ. 2012 Reduction in Ki-67 in
benign breast tissue of high risk premenopausal women with the SERM acolbiphene. Annual
Meeting of the American Society of Clinical Oncology, June 1-5, 2012, Chicago, IL.
50. Petroff BK. 2012 Effects of tamoxifen on markers of follicular health in rats treated with
cancer chemotherapy. Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
51. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice. .
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
52. Li S, Hidaka BH, Harvey KE, Sullivan DK, Klemp JR, Carlson SE, Petroff BK, Kimler BF, Fabian CJ.
2012 Breast cancer risk biomarkers are associated with dietary intake and tissue content of n-3
polyuns turated fatty acids. Annual Meeting of the International Society for the Study of Fatty
Acid Lipids, May 26-30, 2012, Vancouver, CA.
53. Delman, D., Fabian, C.J., Kimler, B.F., Petroff, B.K. 2013. Flaxseed lignan prevents early changes
of mammary carcinogenesis in rats at high risk for breast and ovarian cancer. Annual Meeting of
the American Association for Cancer Research, April 6-10, 2013, Washington D.C.
54. Delman, D. and Petroff, B.K. Mechanisms of follicular rescue by tamoxifen in
cyclophosphamide-treated rat ovaries. 2013. Annual Meeting of the Society for the Study of
Reproduction. July 25, 2013.
55. Li S, Hidaka BH, Harvey KE, Sullivan DK, Klemp JR, Carlson SE, Petroff BK, Kimler BF, Fabian CJ.
2012 Breast cancer risk biomarkers are associated with dietary intake and tissue content of n-3
polyunsaturated fatty acids. Annual Meeting of the International Society for the Study of Fatty
Acid Lipids, May 26-30, 2012, Vancouver, CA.
56. Fabian CF, Kimler BF, Petroff BK, Zalles CM, Metheny T, Box JA, Nydegger JL, Phillips TA, Hidaka
BH, Carlson SE, deGraffenried LA, Hursting SD: High dose omega-3 fatty acid (FA)
supplementation modulates breast tissue biomarkers in pre-menopausal women at high risk
for development of breast cancer. J Clin Oncol 31, 2013 (suppl; abstr 1515).
57. Fabian CJ, Kimler BF, Petroff BK, Zalles CM, Metheny T, Nydegger JL, Box JA, Phillips TA, Hidaka
BH, Carlson SE, deGraffenried LA, Hursting SD: High dose omega-3 fatty acid supplementation
modulates breast tissue biomarkers in post-menopausal women at high risk for development of
breast cancer. 2013 San Antonio Breast Cancer Symposium. 10-14 Dec 2013. P4-10-01.
58. Fabian CJ, Kimler BF, Nydegger JL, Metheny T, Zalles CM, Petroff BK, Yeh H, Alvarado MD. Initial
report of a randomized trial of letrozole in high risk women taking hormone replacement
therapy. 2014 San Antonio Breast Cancer Symposium.
Poster presentation at local and regional meetings:
1. “The role of prostaglandin F2a in uterine and ovarian physiology”, Department of Physiology
Seminar Series, University of Kansas Medical Center, 6/23/98.
2. “Interactions between estrogens and dioxins in the female rat”, Department of Physiology
Seminar Series, University of Kansas Medical Center, 7/25/99.
3. “Interactions between the female endocrine system and the aryl hydrocarbon receptor
pathway”, Molecular and Integrative Physiology, KUMC, 10/21/02.
4. “Interactions between the female endocrine system and the aryl hydrocarbon receptor
pathway”, Anatomy and Cellular Biology, KUMC, 11/12/02.
Curriculum Vitae
Petroff, Brian K.
P a g e | 27
5. “The aryl hydrocarbon receptor pathway and female reproductive health”, Donald Johnson
seminar series, KUMC, 06/12/04
6. “The aryl hydrocarbon receptor pathway and reproductive aging”, Hematology Oncology,
KUMC, 12/06/04.
7. “Theory and practice of immunoassay during biomedical research”, Internal Medicine, KUMC,
12/02/05.
8. “Reproductive aging and environmental toxicants”, Hematology and Oncology, KUMC, 3/21/05
9. “The aryl hydrocarbon receptor and infertility in polluted environments”, Donald Johnson
Seminar Series, University of Kansas Medical Center, Kansas City, KS, 3/1/06
10. Ting AY, Valdez KE, Xue Q, Kimler BF, Fabian CJ, Petroff BK Development of a rat model of
simultaneous mammary and ovarian cancer. October 2005, The Greenwald Symposium, Kansas
City, KS.
11. “An update on SERMs for cancer prevention and survivorship”. DC Johnson Seminar Series, KU
Medical Center, Kansas City, KS, 11/12/08.
12. Valdez KE, Kimler BF, Fabian CJ, Petroff BK. A proteomic assessment of serum and fine needle
aspirate specimens to identify increased risk and response to breast cancer prevention
strategies. In: Program of the Kansas City Area Life Science’s Research Day, Overland Park, KS,
2005. Abstract G3.
13. Ting A, Petroff BK. A preclinical model of simultaneous breast and ovarian cancer. The 3rd
Annual Gilbert S. Greenwald Symposium on Reproduction (platform presentation), October,
2006, Kansas City, MO.
14. “Mechanisms of premature reproductive senescence in females”, Anatomy departmental
seminar series, University of Kansas Medical Center, 1/4/07.
15. Petroff, BK 2008 Stem cells and breast cancer prevention. Annual Research Meeting of the KU
Cancer Center, Lawrence, KS.
16. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice. .
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
17. Ting AY and Petroff BK. Protective Effect of Tamoxifen Against Ovarian Follicle Loss Caused by
Multiple Ovotoxic Agents. April 2008. Department of Molecular & Integrative Physiology,
University of Kansas Medical Center, Kansas City, KS.
18. Ting AY and Petroff BK. Tamoxifen rescues the ovarian follicular reserve following exposure to
multiple classes of ovotoxic drugs including cancer chemotherapeutics. 2008 Student Research
Forum, University of Kansas Medical Center.
19. Petroff BK. A novel approach for fertility preservation in cancer patients. Internal Medicine
Grand Rounds Translational Medicine conference, University of Kansas Medical Center,
4/09/08.
20. Petroff, BK 2008 Stem cells and breast cancer prevention. Annual Research Meeting of the KU
Cancer Center, Lawrence, KS.
21. Petroff BK. 2012 Effects of tamoxifen on markers of follicular health in rats treated with
cancer chemotherapy. Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
22. Warren, BD, Jasti S, Petroff BK, Petroff MG. 2012. The Autoimmune Regulator (AIRE) protects
against infertility, reproductive tract inflammation and germ cell loss in male Balb/c mice.
Annual Greenwald Symposium, Oct 11-12, 2012. Kansas City, MO.
Invited seminars at other universities:
Provide title, sponsoring organization or institution, and date of presentation.
1.
“Polycystic vs. cystic ovarian disease: two pathways towards ovulation failure”, Advanced
Reproductive Physiology Seminar Series and Physiology of Reproduction course, Department of
Animal Sciences, Ohio State University, 3/10/96 and 3/20/96.
Curriculum Vitae
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Petroff, Brian K.
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“Prostaglandin F2 actions during parturition and luteolysis”, Polish National Academy of
Sciences, Division of Reproductive Endocrinology, Olsztyn, Poland, 4/14/98.
“Systemic vs. local ovarian interactions between dioxins and estrogens in the female rat”, College
of Veterinary Medicine, Tokyo University of Agriculture and Technology, 4/13/00.
“A young scientist’s perspective on biological training in the USA”. Graduate student society,
University of Warmia and Mazury, Olsztyn, Poland, 06/10/02.
“Disruption of the female endocrine system by dioxins”. Polish Physiological Society, University of
Warmia and Mazury,Olsztyn, Poland, 06/11/02.
“Disruption of the female endocrine system by dioxins”, Dept. of Zoology, Jagiellonian University,
Krakow, Poland, 06/13/02.
“Interactions between the AhR and prolactin systems”. University of Warmia and Mazury,Olsztyn,
Poland, 03/12/04.
“The aryl hydrocarbon receptor pathway and female reproductive aging”. Dept. of Psychology,
West Chester College, West Chester, PA, 03/12/05.
“Genetic analysis of formalin-fixed tissues for breast cancer biomarker discovery”, featured on
website of Arcturus, 10/05-12/10.
“Loss of fertility with chronic exposure to AhR ligands”, Central European Conference on
Reproduction, Jagiellonian University, 09/12/05.
“Breast biomarker assessment by laser capture, RNA amplification and real time PCR in benign
breast tissue sampled by random periareolar fine needle aspiration”. Arcturus Users Meeting, La
Jolla, CA, 09/18/05
“Breast biomarker expression in benign breast tissue sampled by RPFNA and measured by LCM,
RNA amplification and real time PCR. Arcturus AG, Web seminar, 11/10/05.
“DNA and RNA isolation and real time PCR”, Kansas Masonic Cancer Research Institute,
02/15/06
“Reproductive senescence following persistent activation of the aryl hydrocarbon receptor
pathway in polluted environments”, Frontiers in Reproduction annual symposium, Marine
Biological Laboratory, Woods Hole, MA, 06/17/06.
“AhR pathway and senescent infertility in polluted environments”, Frontiers in Reproduction
Summer Course, Woods Hole Research Institute, 7/16/06
“Reproductive aging in polluted environments”, Ohio State University College of Veterinary
Medicine, 4/30/07.
“Getting old before your time: the AhR and the menopause”, Midwest Research Institute,
Director’s Seminar Series; Kansas City, MO, 8/07/07.
“The aryl hydrocarbon receptor and infertility in polluted environments”, Donald Johnson
Seminar Series, University of Kansas Medical Center, Kansas City, KS, 3/1/06
“Veterinary medicine and human biomedical research”, College of Veterinary Medicine, Kansas
State University, Manhattan, KS, 6/29/06.
“AhR pathway and senescent infertility in polluted environments”, Frontiers in Reproduction
Summer Course, Woods Hole Research Institute, 7/16/06
“Mechanisms of premature reproductive senescence in females”, Anatomy departmental seminar
series, University of Kansas Medical Center, 1/4/07.
“Biomarker gene expression in breast epithelial cells harvested by RPFNA and DL followed by laser
capture microdissection”, 4th biannual meeting of Dr. Susan Love Research Foundation, Santa
Monica, CA, 3/3/07.
“Reproductive aging in polluted environments”, Ohio State University College of Veterinary
Medicine, 4/30/07.
“Getting old before your time: the AhR and the menopause”, Midwest Research Institute,
Director’s Seminar Series; Kansas City, MO, 8/07/07.
“Early TCDD exposure effects on reproductive cyclicity and early reproductive senescence”, Gordon
Research Conference on Endocrine Disruptors, Waterville, NH, 06/16/08.
Curriculum Vitae
Petroff, Brian K.
P a g e | 29
26. “Stem cell number as a measure of breast cancer risk”. KUCC Annual Symposium, University of
Kansas, Lawrence, KS, 11/08/08.
27. “Cancer prevention strategies in human and veterinary medicine”, Frostbite Veterinary
Conference, Overland Park, KS, 02/22/09.
28. “Cancer Prevention”, Mensa Society, Kansas City, KS. 7/05/09
29. “Mechanisms of infertility in polluted environments”, Department of Animal Sciences, Rutgers
University, New Brunswick, NJ, 10/02/09”.
30. Teaching sabattical, 01/11-02/11, European Union Human Capital Program, European Social
Fund, 30 hours of lecture.
31. “Characterization and prevention of infertility from environmental endocrine disruptors”. Polish
Physiological Society, University of Warmia and Mazury, Olsztyn, Poland, 05/17/09.
32. “Effects of endocrine disruptors on female reproductive function – animal and human”. 5th
Copenhagen Workshop on Endocrine Disruptors, Copenhagen, Denmark, 05/20/09.
33. “Cancer prevention”, Mensa Society, Kansas City, KS 7/05/09
34. “Mechanisms of infertility in polluted environments”, Department of Animal Sciences, Rutgers
University, New Brunswick, NJ, 10/02/09.
35.
“Effect of chronic dioxin exposure on reproduction in the female rat. Annual Meeting of the
Society for the Study of Reproduction. Aug 1-3, Milwaukee, WI. Biol Reprod (Suppl 1), abstract
209.
36. “Preventing infertility with environmental and chemotherapeutic toxicities”. Eur Physiol Society,
Olsztyn, Poland.
37. “Flaxseed lignan for breast and ovarian cancer prevention.” Komen for the Cure Annual Meeting,
Washington, DC.
38. “Effects of dioxins on female reproductive aging”. University of Illinois CVM, 07/30/12.
39. “Fetal origins of adult disease”. University of Illinois 07/31/12.
40. “Effects of flaxseed lignan SDG on early breast and ovarian cancer progression”. Komen for the
Cure Annual Meeting. Dallas, TX. 03/09/13.
41. Secoisoresorecinol diglycoside (SDG, flaxseed lignan) improves biomarkers of early breast cancer
progression in rats at high risk for breast and ovarian cancer. AACR Annual Meeting, Washington
DC. 04/08/13.
42. “Endocrinology and ovotoxicity from AhR ligands”. Michigan State University. 10/01/13.
4. Other Evidence of Scholarship:
Includes clinical guidelines, policy documents, contributions to significant position statements by
professional organizations, and development of national examinations.
Commercial applications of research activities
KUMC Invention disclosure: A rodent model for the development of dual target chemoprevention drugs
against breast and ovarian cancer. 2006. Petroff, B.K., Ting, A., Kimler, B.K., Fabian, C.J.
US Provisional Patent Application # 17469.11: Novel Fertility Preservation for Women with Cancer. Filed
1May08. Brian Petroff D.V.M. Ph.D. and Ms. Alison Ting, co-inventors.
Compan Pharmaceuticals: Long acting pain relief for veterinary patients., IP and startup funding
application. 2013.