Chromatography Lab set up
Problem/Question: To examine solutes in the chromatography process. To
determine Rf factors of each substance derived from a sample. To determine how
many materials are contained in each of the substances separated.
Directions:
Describe the chromatography process through the use of capillary action. (You will
use a fibrous paper and a thin layer coated paper on which samples will travel.)
Use text to do this. Diagrams are helpful. Describe Rf factor and how it defines
material separation. What are the limitations of the Rf factor and the method used
in the lab?
When is TLC an appropriate use of sample separation and /or identification?
Each student includes one paper strip on the lab paper.
Experimental Procedure:
Construct steps of procedure and data table(s) based on the usage of solvent and
solute
Rf factors for each sample
Rf= distance traveled by substance/ distance traveled by
Solvent fractional
You will use thin-layer process with chlorophyll pigments and
paper chromatography for pen ink.
Results
Conclusions/ discussion: make sure to include the paper strip or thin-layer strip and
see questions above to include in discussion.
1. *Describe the chromatography process through the use of capillary action. (You
will use a fibrous paper on which samples will travel and pre- made tlc
sheets.
Use text. See chapter 5 on organic analysis. Do ch. 5 questions as well as
items #2-5 below.
2. *Describe Rf factor and how it defines materials.
Rf= distance traveled by substance/ distance traveled by solvent
3. *What are the limitations of the Rf factor and the method used in the lab?
4. *When is TLC an appropriate use of sample separation and /or identification?
5. *Each student includes one chromatographic paper or tlc strip on the lab paper
to be submitted.