README.

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RAW DATA README FOR Data Accessibility for Manuscript MEC-14-1210.R1:
Paternal epigenetic effects of population density on locust phase-related
characteristics associated with heat-shock protein expression.
Bing Chen, Shaoqin Li, Qiang Ren, Xiwen Tong, Xia Zhang, Le Kang
DATAFILE: Egg weight_Paternal effects_Crowding vs isolation_201408. xlsx
Note this datafile presents data about paternal effects of density changes on egg
weight. The spreadsheet “Crowding effects” shows the effect of paternal crowding
rearing on egg weight. The spreadsheet “Isolation effects” shows the effects of paternal
isolation rearing on egg weight.
Column A =Density treatment: G♀ × G♂ (control) versus G♀ × Gisol♂ for isolation
effect, and S♀ × S♂ (control) versus S♀ × Scrowd♂ for crowding effect. 1 represents S♀
× S♂, 2 reprents S♀ × Scrowd♂, 3 represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = Egg pod: consecutive egg number.
Column C = Mating line: replicate mating lines.
Column D = Egg weight: egg weight of seven-day-old egg. The unit is mg.
DATAFILE: Egg weight_Maternal effects_Crowding vs isolation_201402.xlsx
Note this datafile presents data about maternal effects of density changes on egg
weight. The spreadsheet “Crowding effects” shows the effect of maternal crowding
rearing on egg weight. The spreadsheet “Isolation effects” shows the effects of maternal
isolation rearing on egg weight.
Column A =Density treatment: G♂ × G♀ (control) versus G♂ × Gisol♀, and S♂ × S♀
(control) versus S♂ × Scrowd♀. 1 represents S♀ × S♂, 2 reprents S♀ × Scrowd♂, 3
represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = Egg pod: consecutive egg number.
Column C = Mating line: replicate mating lines.
Column D = Egg weight: egg weight of seven-day-old egg. The unit is mg.
DATAFILE: Hsp gene expression_in Egg_Paternal effects_201407.xlsx
Note this datafile presents the effect of PATERNAL crowding or islation on expression
of Hsp genes in offspring, i.e., Hsp90, Hsp70 and Hsp20.6, which are shown in three
spreadsheets.
Column A = Density treatment: G♀ × G♂ (control) versus G♀ × Gisol♂ for isolation
effect, and S♀ × S♂ (control) versus S♀ × Scrowd♂ for crowding effect. 1 represents S♀
× S♂, 2 reprents S♀ × Scrowd♂, 3 represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = qPCR replicate: each sample have three technical replicates on PCR
plate, i.e., 1, 2, 3.
Column C = Ct_Rp49: The threshold cycle value (Ct) for the amplification of the internal
control gene Rp49.
Column D = Averaged Ct_Rp49: The avaraged value of three technical replicates
Column E = Ct_Hsp: The threshold cycle value (Ct) for the amplification of Hsp gene.
Column F = Averaged Ct_Hsp: The avaraged value of three technical replicates
Column G = ∆Ct: the vaule of Averaged Ct_Hsp minus Averaged Ct_Rp49.
Column H = 2^(-∆Ct): the value of 2^(-∆Ct). This value was used as the final value for
mRNA level of each sample.
DATAFILE: Hsp expression in Egg_Maternal effects_201301.xlsx
Note this datafile presents the effect of MATERNAL crowding or islation on expression
of Hsp genes in offspring, i.e., Hsp90, Hsp70 and Hsp20.6, which are shown in three
spreadsheets.
Column A = Density treatment: G♂ × G♀ (control) versus G♂ × Gisol♀, and S♂ × S♀
(control) versus S♂ × Scrowd♀. 1 represents S♀ × S♂, 2 reprents S♀ × Scrowd♂, 3
represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = qPCR replicate: each sample have three technical replicates on PCR
plate, i.e., 1, 2, 3.
Column C = Ct_Rp49: The threshold cycle value (Ct) for the amplification of the internal
control gene Rp49.
Column D = Averaged Ct_Rp49: The avaraged value of three technical replicates
Column E = Ct_Hsp: The threshold cycle value (Ct) for the amplification of Hsp gene.
Column F = Averaged Ct_Hsp: The avaraged value of three technical replicates
Column G = ∆Ct: the vaule of Averaged Ct_Hsp minus Averaged Ct_Rp49.
Column H = 2^(-∆Ct): the value of 2^(-∆Ct). This value was used as the final value for
each sample.
DATAFILE: Larva morphology_Paternal effects_201408.xlsx
Note this datafile presents data about paternal effects of density changes on
morphometric traits in offspring larvae. The spreadsheet “Paternal effects_crowding”
shows the effect of paternal crowding rearing on morphological traits. The spreadsheet
“Paternal effects_Isolation” shows the effects of paternal isolation rearing on
morphological traits.
Column A =Density treatment: G♀ × G♂ (control) versus G♀ × Gisol♂ for isolation
effect, and S♀ × S♂ (control) versus S♀ × Scrowd♂ for crowding effect. 1 represents S♀
× S♂, 2 reprents S♀ × Scrowd♂, 3 represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = Mating line: replicate mating lines.
Column C = F (mm): hind femur length.
Column D = C (mm): maximum head width
Column E = F/C: the ratio of F and C.
Column F = ln(F/C): :tranformation of the ratio F/C with the function ln().
DATAFILE: Larva morphology-Maternal effects-201311.xlsx
Note this datafile presents data about maternal effects of density changes on
morphometric traits in offspring larvae. The spreadsheet “Maternal effects_crowding”
shows the effect of maternal crowding rearing on morphological traits. The spreadsheet
“Maternal effects_Isolation” shows the effects of maternal isolation rearing on
morphological traits.
Column A =Density treatment: G♂ × G♀ (control) versus G♂ × Gisol♀, and S♂ × S♀
(control) versus S♂ × Scrowd♀. 1 represents S♀ × S♂, 2 reprents S♀ × Scrowd♂, 3
represents G♀ × G♂, 4 represents G♀ × Gisol♂.
Column B = Mating line: replicate mating lines.
Column C = F (mm): hind femur length.
Column D = C (mm): maximum head width
Column E = F/C: the ratio of F and C.
Column F = ln(F/C): tranformation of the ratio F/C with the function ln().
DATAFILE: Parental adult coloration-201301.xlsx
Note this is the data file about the coloration of male adult in parental generation.
Column A =Density treatment: there are four different treatments. 1 represents
solitarious locusts, 2 represents crowded rearing of solitarious locusts, 3 represents
gregarious locusts, and 4 represents isolated rearing of gregarious locusts.
Column B= Sample no: the number of measured adults.
Column C = color score: Gregarious adults exhibit gray to black patterns in the thorax
pronotum and pleurite. By contrast, solitarious adults show a green pattern (green or
brown coloration). Here I represents black, and 2 represents green or brown color.
DATAFILE: Parental adult morphology-201301.xlsx
Note this is the data file about the morphology of male adult in parental generation.
Column A = Density treatment: there are four different treatments. 1 represents
solitarious locusts, 2 represents crowded rearing of solitarious locusts, 3 represents
gregarious locusts, and 4 represents isolated rearing of gregarious locusts.
Column B= Sample no: the number of measured adults.
Column C = E (mm): tegmen length.
Column D = F (mm): hind femur length.
Column E = C (mm): maximum head width.
DATAFILE: Parental expression of Hsp genes in BRAIN-201309.xlsx
Note this datafile presents the expression of Hsp genes in BRAIN in parents caused by
crowding or islation. The three Hsp genes, i.e., Hsp90, Hsp70 and Hsp20.6, are shown
in three spreadsheets.
Column A = Density treatment: Density treatment: there are four different treatments. 1
represents solitarious locusts, 2 represents crowded rearing of solitarious locusts, 3
represents gregarious locusts, and 4 represents isolated rearing of gregarious locusts.
Column B = qPCR replicate: each sample have three technical replicates on PCR
plate, i.e., 1, 2, 3.
Column C = Ct_Rp49: The threshold cycle value (Ct) for the amplification of the internal
control gene Rp49.
Column D = Averaged Ct_Rp49: The avaraged value of three technical replicates
Column E = Ct_Hsp: The threshold cycle value (Ct) for the amplification of Hsp gene.
Column F = Averaged Ct_Hsp: The avaraged value of three technical replicates
Column G = ∆Ct: the vaule of Averaged Ct_Hsp minus Averaged Ct_Rp49.
Column H = 2^(-∆Ct): the value of 2^(-∆Ct). This value was used as the final value for
mRNA level of each sample.
DATAFILE: Parental expression of Hsp genes in LEG-201309.xlsx
Note this datafile presents the expression of Hsp genes in LEG muscle in parents
caused by crowding or islation. The three Hsp genes, i.e., Hsp90, Hsp70 and Hsp20.6,
are shown in three spreadsheets.
Column A = Density treatment: Density treatment: there are four different treatments. 1
represents solitarious locusts, 2 represents crowded rearing of solitarious locusts, 3
represents gregarious locusts, and 4 represents isolated rearing of gregarious locusts.
Column B = qPCR replicate: each sample have three technical replicates on PCR
plate, i.e., 1, 2, 3.
Column C = Ct_Rp49: The threshold cycle value (Ct) for the amplification of the internal
control gene Rp49.
Column D = Averaged Ct_Rp49: The avaraged value of three technical replicates
Column E = Ct_Hsp: The threshold cycle value (Ct) for the amplification of Hsp gene.
Column F = Averaged Ct_Hsp: The avaraged value of three technical replicates
Column G = ∆Ct: the vaule of Averaged Ct_Hsp minus Averaged Ct_Rp49.
Column H = 2^(-∆Ct): the value of 2^(-∆Ct). This value was used as the final value for
mRNA level of each sample.
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