SPECIFICATIONS OF HPLC SYSTEM
HPLC SYSTEM (High Pressure BINARY GRADIENT.)
(COUNTRY of Origin: JAPAN, Germany, USA)
1. PUMPS 2 NOs
2. Gradient Mixer
3. PDA DETECTOR
4. Auto Sampler
5. Column
6. System Controller
7. PC and Printer
8. Software
9. UPS 3KVA or higher suitable
TECHNICAL SPECIICATIONS:
1. HIGH PRESURE PUMP Qty 2
Flow rate
0.001 to 10mL
Max. pressure
40MPa
Flow rate Precision
0.06% RSD
Safety Measure
Leak sensor, High Pressure
/low pressure limit
Solvent Delivery
Serial type double plunger
Constant pressure Solvent
delivery
Supported
Typical Pulsation
0.08MPa
2. PDA DETECTOR
Wavelength range
190 nm to 800nm
Light Source
D2 lamp and Tungsten Lamp
Number Of diode elements
512
Bandwidth, slit width
8 nm (High sensitivity mode), 1.2 nm
high resolution mode.
Wavelength accuracy
+ 1nm
Wavelength precision
+ 0.1nm max.
Noise
0.6x10-5 AU
Drift
5x10-4AU/Hr
Cell Temp setting range:
OFF, 5 deg above ambient to 50degC
3. AUTO INJECTOR
Injection Volume Setting range
0.1 to 100uL
Number Of processed samples
175 (1mL vials), 105 (1.5 mL Vials, 50
(4 mL vials)
Injection Volume accuracy
1% maximum
Injection volume precision
RSD:0.3%
Sample carry Over
0.005% (Specified condition), 0.0025%
typical condition
Number Of repeated injection
30 max per sample
Operating PH
pH1 to pH14
Needle rinsing
Set freely before and after sample
injection
4. Columns
ODS C-18
Qty 3
Normal Phase Silica Column
C-30 analytical Column
Qty 2
Qty 1
5. PC and HP Laser Jet Printer
6. Software
1.
2.
3.
4.
5.
6.
Operation of the system should be very easy and intuitive via a state-of-the-art 32
bit Windows based software
It should be Client/Server based
It should be capable of control of HPLC systems and data acquisition of up to 4
channels. It should cover full one-point digital instrument control, qualitative and
quantitative processing, report creation and self-diagnosis
Sample schedule wizard function should be standard
There should be an on-line help function context sensitive
The reporting format should be flexible and easy to use in any desired format
The data can be converted to other formats. Spread Sheet software and wordprocessing software can be readily employed to provide data in tables or graphs
through industry standard protocols
7. The software should allow automatic execution of system checks, auto-purge and
baseline checks
8. Software must have its own log files for complete audit trails
9. System suitability, System security as well as System check functions must be
provided which comply with Good Laboratory Practice (GLP) and Regulatory
Conformity
SPECIFICATIONS FOR CHEMICALS
The chemicals should be from Sigma or Equivalent.
Chemicals or Reagents
3-keto5-cholestene
7-ketocholesterol
Acetic acid
Acetone
Alpha-tocopherol
Ammonium acetate
Ascarbic acid
a-tocopheryl acetate
Butylated hydroxytoluene
Chloroform
Cholesterol
Cholesterol epoxide
Cholesterol kit
DPPH
Ethanol
Ethyl acetate
Glucose kit
GST kits
HDL-c kit
Linoleic acid
Magnesium chloride
Methanol
Methyl tert butyl ether
Oleic acid
Perchloric acid
Peroxidase
Potassium chloride
Potassium hydroxide
Sodium acetate
Sodium hydroxide
Thiobarbituric acid
Tocopherol
Triglycerides Kit
Quantity
100 mg
100 mg
5L
10 L
100 g
250 g
500 g
100 g
1 kg
5L
50 g
50 mg
2 kits
2g
10 L
2L
5 kits
1 kit
2 kits
25 g
1 kg
50 L
10 L
25 g
500 mL
100 KU
500 mg
1000 g
1 kg
1 kg
100 g
500 g
2 kits
Unit Rate (Rs)
Total Price (Rs)
Trilenolein
Tripalmitin
50 g
50 g
SPECIFICATIONS FOR GLASSWARE’S
Glass wares/plastic wares
pH strips
Gloves
Erlenmeyer flasks (different volume)
Graduate cylinders (different volume)
Pipette (different volume)
HPLC vials with caps
Pipette pump
Centrifuge tubes (different volume)
Racks
Syringes (different size, volume)
Laboratory tool sets
Laboratory tubes (different size)
Glass bottles (different volume)
Tubing Fitting combination set
Absorption cuvettes
Para film M
Blood collection tubes
Pipette tips (different size)
Micro pipette controller
Watman filter paper
Mini shaker
Quantity
2
10
100
50
10
1000
5
1000
10
1000
2
50
100
2
4
2
1000
2000
5
20
1
Unit Price (Rs)
Total Price (Rs)