The Effects of Carbon Dioxide on Brine Shrimp Development
Hypothesis:
Procedure:
Brine shrimp cysts were subjected to liquid carbon dioxide and compared to a control without liquid
carbon dioxide. Brine shrimp cysts were placed in mason jars. Each container helld 800 ml of
dechlorinated water and 1.00 g brine shrimp cysts. The Control contained water at PH 8.0 and salinity
consisting of 2 Tablespoons ocean salt dissolved in 1 Quart of dechlorinated water. Each container was
be placed under plant lights and aerated. PH was adjusted by the addition of white vinegar and/or
baking soda. Two control jars and two test jars were setup. Two (2) drops of liquid carbon dioxide will be
added to each jar daily during the school week.
Samples were taken by taking two drops of water and counting live brine shrimp under a stereotypic
microscope. PH was measured each time shrimp were counted. Carbon dioxide was also measured
once. Temperature under the plant lights was also recorded.
Temperature (oC) Under Plant Ligbts:
Setup Date:
Jar Number
Treatment
(Control or Test)
PH
Carbon Dioxide
1
2
3
4
Daily Data Tables:
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Date:
Temperature (oC) Under Plant Ligbts:
PH
Carbon Dioxide
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Date:
Temperature (oC) Under Plant Ligbts:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
PH
Carbon Dioxide
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
Count/Drop
(sample #1)
Count/Drop
(sample #2)
1
2
3
4
Temperature (oC) Under Plant Ligbts:
Date:
Jar Number
1
2
3
4
Count/Drop
(sample #1)
Count/Drop
(sample #2)