University AlaskaAnchorage Anchorage University of of Alaska Graduate Studies and Research Summit Graduate Studies and Research Summit April 8, 2011 April 8, 2011 Conoco Philips Integrated Science Sponsored by Building UAA Graduate Student Association And The Union of Students (USUAA) by InSponsored Partnership with UAA Graduate Student Association UAA’s Environment and Natural Resources Institute (ENRI) & The Union of Students (USUAA) In Partnership with UAA’s Environmental and Natural Resources Institute (ENRI) About the Graduate Student Association What is GSA? The Graduate Student Association (GSA) is an association for the organization and advocacy of graduate students at UAA. Student members are currently sitting on numerous university advisory boards. The GSA hosts social gatherings to encourage graduate student communication and organization. The GSA also offers research and professional funding opportunities each semester. Who can get involved with GSA? Membership in the GSA is open to all students. How to get involved: Register to the GSA listserve (gsa@lists.uaa.alaska.edu) Attend GSA meetings Present your research by participating in the graduate student summit Apply for funding opportunities Become member of a campus committee Discuss and advocate graduate student issues Plan and attend GSA organized activities When does GSA meet? Meeting times and locations are announced through the mailing list (gsa@lists.uaa.alaska.edu) by the Coordinator. Meetings will occur at least three times per semester. Any member may raise a topic for discussion. How to know when the meetings are happening: To receive meeting information, prospective members need to register for the GSA listserve by completing the form at http://lists.uaa.alaska.edu/mailman/listinfo/gsa. Current members can contact all other registered members by simply emailing gsa@lists.uaa.alaska.edu. How to apply for GSA funding: GSA requests proposals for awards to assist advanced level studies and research. Request for proposals usually happen once a semester, however, that may be funding dependent. Awards are available per currently enrolled UAA student or per research project (in the case that more than one applicant requests funding for the same project). How the GSA funding can be used: GSA funds can be awarded for supplies necessary to conduct research, travel for business related to education goals, professional society memberships and anything else that enhances a student’s program of study. For more information visit: http://www.uaa.alaska.edu/gsa/ or http://uaa.collegiatelink.net/organization/gsa 2 Acknowledgements The accomplishments of the UAA Graduate Student Association and the Graduate Studies and Research Summit would not have happened without the hard work, dedication, and vision of great students, faculty, and administrators. Students Becky Sawyer –coordinator Lisa Ebbs –treasurer Emily Lescak –secretary Leah Kenney and Elizabeth Sharp – ENRI liaisons Erin Trimble and Kim Jochum - Graduate Council representatives Rachel Boschma-Wynn - Technology chair Dawn Copell - USUAA representative Lynda Hernandez - MPH representative Lauren Caruso - biology graduate student representative Cody Chipp – co-organizer for Applying to Graduate School workshop, previous coordinator Scholarship Reviewers Summit Volunteers All the graduate students who attended the meetings and gave their insight and creative ideas to help us achieve success Faculty Dr. Jeffery Welker – Professor and Director of ENRI The GSA would like to thank Dr. Welker for his vision and inspiration in partnering ENRI with UAA’s Graduate Student Association, as well as supporting graduate education at UAA. Poster session judges Panelists for the Applying to Graduate School workshop Administration Anita Bradbury – Administrative Assistant for USUAA Paula Uturbey-Fish - Assistant director of student leadership For their guidance and assistance with the scholarship Kathy Boeckman - Administrative Assistant to the Director of ENRI For assistance with event advertising Elisa Mattison – Director of UAA Graduate School For her dedication to and support of graduate students Robert White – Former Dean of UAA Graduate School & Vice Provost for Research David Yesner – Associate Dean of the Graduate School For their advocacy for graduate students, participation in events, and support for graduate education at UAA 3 Door Prize Donors Moose’s Tooth Gemmes du Terre UAA General Support Services UAA Music Department UAA Bookstore Cinemark Campus Map Oral presentations will take place at the Allied Health Sciences (AHS) Building. The poster session and mixer will be held at EBL. 4 5 Schedule Time Presentations in AHS 106 9:00 Introductions/Welcome AHS 106 CO2 exchanges in High Arctic A survey of blood parasites from ecosystems: responses to multiple southern Alaskan brown and black levels of warming and increases in bears using microscopic and summer precipitation, NW molecular techniques Greenland Becky Sawyer Department of Biological Sciences Elizabeth Sharp Department of Biological Sciences Habitat-specific foraging drives Testing the conservation of the mercury concentrations in benthic mammalian WINAC and B-WICH and limnetic morphotypes of ATP-dependent remodeling stickleback in Benka Lake, Alaska complexes in Xenopus laevis James Willacker Nicole Stopa Department of Biological Sciences Department of Biological Sciences To reproduce or not: NutritionAn evaluation of the current hormonal influence on reproductive methods of monitoring annual decisions in seasonally synchronous production of Midcontinent Greater breeders White-fronted Geese (Anser Cory Stantorf albifrons) that breed in Interior and Department of Biological Sciences Northwest Alaska Wade Schock Department of Biological Sciences 9:30 9:50 10:10 10:30 11:00 11:20 11:40 Presentations in AHS 147 Coffee Break and Door Prizes Behavioral health aide manual ethics Partitioning ecosystem respiration in Matthew R Sena arctic tussock tundra to more Department of Clinical-Community accurately forecast changes in the Psychology carbon cycle Aliza Segal Department of Biological Sciences Measuring resilience among Somali Water sources of evergreen and refugees living in the United States deciduous species depend upon Rebecca Volino Robinson season, ecosystem type and Program in Clinical-Community snowpack depth in arctic tundra Psychology near Toolik Lake, Alaska Lisa Ebbs Department of Biological Sciences Trauma in schizophrenia disorders: Genomics underlying rapid Asking the question phenotypic change in threespine Ann K. Yates stickleback populations from Prince Program in Clinical-Community William Sound and the Gulf of 6 Psychology 12:00 12:30 1:30 1:50 2:10 2:30 3:00 3:30 4:00 5:007:00 Alaska Emily Lescak Department of Biological Sciences Plenary Speaker – Paula Donson AHS 106 Lunch Break Life meaning among rural Alaska Confronting bear management in Native and American Indian college the changing North – future foci for students efficient sustainable adaptive Tonie Quaintance management strategies Program in Clinical-Community Kim Jochum Psychology RAM Group, Biological Sciences, University of Alaska Anchorage Barriers and facilitators to self-care Recent phenotypic and molecular in graduate students evolution of trophic polymorphism Karin Sandberg and Rebecca Volino in a species pair of kokanee Robinson (Oncorhynchus nerka) in southwest Program in Clinical-Community Alaska Psychology Kyle Shedd Department of Biological Sciences Health-related behaviors of firstUnderstanding moose foraging year university students behavior in response to variations in Cody Chipp patch architecture and spatial motifs Center for Behavioral Health Research Lauren Caruso and Services Department of Biological Sciences Coffee Break and Door Prizes A Hidden Impact of Climate Change on Women’s Health: A Literature Review Linking Violence against Women and Climate Change Jennifer Jones Department of Health Sciences Plenary Speaker – Stephanie McAfee AHS 106 Plenary Speaker - David Yesner AHS 106 Graduate Student Mixer and Poster Session in EBL 7 Posters Increasing NDVI values in northern Alaska: studies that mix shrub density, spectral and CO2 exchange measurements Andy Anderson-Smith Department of Biological Sciences Water sources of evergreen and deciduous species depend upon season, ecosystem type and snowpack depth in arctic tundra near Toolik Lake, Alaska Lisa Ebbs Department of Biological Sciences Why ask about firesetting? A review of the clinical literature on firesetting behavior Valerie Hewell Program in Clinical-Community Psychology Total Hg concentration in freshwater fish at Adak, Island, Aleutian Archipelago, Alaska Leah Kenney Department of Biological Sciences Distribution and ecology of zooplankton and juvenile salmon in the Copper River Plume Laurel McFadden Department of Biological Sciences The Role of Histone H2A in DNA MisMatch Repair David Robinson Department of Biological Sciences Impacts of Melilotus alba on pollinator services, diversity and abundance in the boreal forest of interior Alaska Laura Schneller Department of Biological Sciences Stable isotope (δ13C, δ15N) analysis and satellite telemetry depict the complexity of gray wolf (Canis lupus) diets in Southwest Alaska Ashley Stanek Department of Biological Sciences The effects of hibernation on the gut microbial community of the Arctic Ground Squirrel Tim Stevenson Department of Biological Sciences 8 Abstracts (Listed alphabetically by author’s last name) Increasing NDVI values in northern Alaska: studies that mix shrub density, spectral and CO2 exchange measurements Andy Anderson-Smith1*, Amber Lewis1, Paddy Sullivan1, Jeffrey M. Welker1,2 1 University of Alaska Anchorage, Department of Biological Sciences 2 University of Alaska Anchorage, Environment and Natural Resources Institute *andersonsmith@gci.net Delineating the mechanisms and consequences of changes in tundra landscapes is central to predicting the functional ecology of Alaska in the 21st Century. Evidence has been mounting during the last decade that shrub communities are expanding in the Arctic and Normalized Difference Vegetation Index (NDVI) values which measure surface greenness are rising. Several studies have suggested that NDVI increases are being driven by increases in shrub abundance. While it is clear that NDVI has increased across vegetation types, it is not clear that NDVI values are increasing in moist acidic tundra (MAT), the most extensive vegetation type in arctic Alaska and the one most likely to be changed by global warming. The MAT is important to large mammal herbivores such as caribou which provide subsistence for indigenous people. The focus of this research is to determine what rising NDVI values actually mean in the MAT. The degree to which tundra plant community composition affects NDVI is still very poorly understood. In order to clarify the role of shrub encroachment per se as opposed to other functional groups in driving increases in NDVI, we measured functional group composition in moist acidic tundra in conjunction with hand-held measures of NDVI and direct CO2 exchange measurements to explicitly link spectral properties, shrub, graminoid and bryophyte density, and trace gas feedbacks to atmospheric chemistry. Point frame data shows a shrub coverage of Betula nana (Dwarf Birch) and Salix pulchra (Diamond Leaf Willow) combined of 5% to 35% in MAT. Our results indicate that high shrub density (>30%) corresponds to peak season NDVI values greater than .75 whereas low shrub density correspond to values below .65 (R2=.66). Furthermore, NDVI is closely correlated with canopy leaf area and greater leaf area is associated with higher rates of gross and net ecosystem CO2 uptake. Understanding moose foraging behavior in response to variations in patch architecture and spatial motifs Lauren Caruso 1*, Don Spalinger 1 1 University of Alaska Anchorage, Department of Biological Sciences *lacaruso@uaa.alaska.edu Understanding the foraging behavior of herbivores is critical to the analysis of habitat requirements of a population or species, and hence, their conservation. Foraging herbivores face a number of decisions in food selection, and the goal of this experiment is to identify the scale at which herbivores makes these decisions in a diverse landscape. 9 This study will investigate the effects of modifying the distribution of browses and its impact on the foraging behavior of moose, an important large herbivore in boreal and arctic ecosystems. We hypothesize that the foraging currency for moose is net rate of energy intake, and that this rate will be maximized. In using mass intake as a proxy for energy intake, we will examine whether energy intake drives foraging behavior by observing how hand-reared moose move within and among simulated patches during a series of feeding trials, in which two browse species of different preference and nutritional value are offered in varying “diluted” proportions. From these results, we should be able to develop functional response curves that mechanistically predict the point at which a foraging herbivore becomes either search-limited or processing-limited by the composition and availability of food types. Health-related behaviors of first-year university students Cody Chipp 1*, Christiane Brems 1, Mark Johnson 1 1* University of Alaska Anchorage, Center for Behavioral Health Research and Services *cchipp@uaa.alaska.edu Over the past 30 years, obesity in the United States has become a health epidemic, with a prevalence rate of overweight and obese adults at nearly 68%. Obesity is a public health concern as it is associated with a higher risk of life threatening chronic illnesses such as diabetes, cardiovascular disease, liver disease, and cancer. Obesity rates have been accelerating over the past two decades nationwide and one crucial time period identified for weight gain is among emerging adults who are starting college. Given that obesity rates have increased in the US, and the transition from high school to college appears to be a time of weight gain, this study examined weight gain among freshmen students based in the context of the psychological constructs of self-efficacy and motivation as related to nutrition and physical activity. Data collected for the study consisted of two waves, a baseline data collection phase and a 24-month follow-up. There were 207 (61% women) respondents at baseline and 89 (43%) completed the follow-up study. Participants completed a series of questionnaires, including self-efficacy measures, stage-of-change measures, physical activity measures, and a Brief Food Frequency Questionnaire. Upon completion of the measures, anthropometric measures were taken (e.g., height, weight, and body fat percentage). Preliminary results indicate that 31% of the sample was overweight or obese at baseline and 26% did not meet physical activity guidelines. Additionally, 30%and 35% of students reported low selfefficacy and low motivation to live a healthyfully, respectively. Further analyses will explore the relationships between students’ BMI, nutritional intake, physical activity levels, and their self-efficacy and motivation to live healthfully when entering college and across time. Using the findings from this study, a series of recommendations will be offered for researchers, university service and care providers, and academic policymakers to address the health needs of new and continuing university students. 10 Water sources of evergreen and deciduous species depend upon season, ecosystem type and snowpack depth in arctic tundra near Toolik Lake, Alaska Lisa Ebbs 1*, Patrick Sullivan1, Andrew Kulmatiski1 and Jeffrey Welker1,2 1 University of Alaska Anchorage, Department of Biological Sciences 2 University of Alaska Anchorage, Environment and Natural Resources Institute *lisaebbs@gmail.com Arctic ecosystems are experiencing rapid increases in surface air temperatures, which are altering the hydrological cycle and its components. In the absence of increased precipitation, these changes will result in surface drying. Recent increases in the frequency and magnitude of tundra fires provide evidence that this change is already occurring. As temperature rises, active layers deepen and soils dry, plant-water relations will become an increasingly important component of the functioning of Arctic ecosystems (previously viewed as water-abundant). Arctic ecosystems are covered by snow for approximately 9 months every year, and yet little is known about the importance of snowmelt as a water source for arctic plants. In this study, we examined seasonal variation in the water sources of two evergreen and two deciduous species in two ecosystems (tussock and dry heath tundra) in response to long-term experimental increases in snow depth. We analyzed δ18O and δD in xylem water and ∆18O, ∆13C and N concentration in leaf tissue, which reveal differences in water sources and in leaf gas exchange physiology. Our findings indicate that snowmelt water may ameliorate the progressive drying of tundra during the summer; however, this is primarily confined to tussock as opposed to dry tundra. Identifying the importance of snowmelt as a source of water for arctic plants will improve our ability to predict changes in plant species composition and ecosystem function in a warmer and drier arctic. Why ask about firesetting? A review of the clinical literature on firesetting behavior Valerie Hewell 1*, Danielle Giroux 1 1 University of Alaska Joint PhD Program in Clinical-Community Psychology *vmhewell@alaska.edu The recently implemented Alaska Screening Tool 2011 (AST2011) may be used to inform the mental health screening and assessment process within the state of Alaska. However, concerns have been raised regarding the utility of including a screening item on firesetting within the AST2011. What might a question on firesetting be able to tell clinicians during the screening process and how might this single item be used as an indicator of other mental health issues and how should it be treated by clinicians if endorsed? In an attempt to answer this complex question, a systematic review of the literature pertaining to firesetting was conducted. An exhaustive literature review of five separate data bases was conducted to find peer-reviewed journal articles published within the field of psychology between January 2000 and October 2010. This search yielded 58 unique articles which met criteria for inclusion. Findings suggest that there are different indicators and implications for children, teens and adults in regard to firesetting. Firesetting is also comorbid with several other diagnoses including substance abuse. 11 Finally, gender differences in firesetting behavior may require different diagnostic and treatment approaches. Confronting bear management in the changing North – future foci for efficient sustainable adaptive management strategies Kim Jochum 1,2*, Lilian Alessa 1, Andrew Kliskey 1, Falk Huettmann 2, Susan Todd 3 1 RAM Group, Biological Sciences, University of Alaska Anchorage ² EWHALE lab, Institute of Arctic Biology, Biology and Wildlife Department, University of Alaska Fairbanks 3 School of Natural Resources and Agricultural Sciences, University of Alaska Fairbanks *kajochum@alaska.edu Resource development and the expansion of industrial activity contribute to problems concerning natural resource use. As a result wildlife has to adapt to increasing rural and urban development. These effects are driven by governmental policies of economic growth, an increasing human population, and the resulting human footprint. In northern ecosystems wildlife in general, but especially bear species and humans alike have tremendous interest in rivers during salmon run season. In some parts of the world strict regulations have been established to regulate fisheries and to assure salmon numbers in rivers meet ecosystem needs (e.g. Alaska). In other regions regulations might exist, however their implementation, the education of local people, and poaching control are absent (e.g. Russian Far East). Additionally during recent years human-bear conflicts have increased in the Russian Far East and in Alaska. Whichever factor is the major trigger - economy, population or ecosystem - such incidences bring into question existing management strategies. Adams et al. (2010) and Woodroffe et al. (2005), among others, conclude that adaptive resilient wildlife management has yet to be accomplished. Its application reveals substantial implementation problems. This study compares the current state-of-knowledge and problems in relation to human-brown bear conflicts along the Pacific Rim (Alaska and Sakhalin Island), identifies reasons for failures in the past, and makes suggestions for improving future brown bear management approaches across regions. Methods are interdisciplinary, including a social science mixed methods approach based on interviews and a follow up survey. Further supervised classification of land use change is used to evaluate historic landscapes and predictive modelling to evaluate suitable brown bear habitat on Sakhalin island. This PhD project will be carried out between 2010 - 2013. Interviews and land use classification are currently conducted. 12 A hidden impact of climate change on women’s health: A literature review linking violence against women and climate change Jennifer Jones University of Alaska Anchorage, Department of Health Sciences Jjones108@alaska.edu Violence against women is a health issue endemic to our society. Recent studies suggest that up to forty percent of Indigenous women will experience violence at some point in their life. In a northern context, such violence becomes entangled in the numerous issues that the circumpolar regions and populations are experiencing as a result of climate change. The purpose of this paper is to use a gendered approach to illuminate current research on climate change and the impact on health. More specifically, it illustrates how climate change and its outcomes are contributing factors to violence perpetrated against Indigenous women in the circumpolar region. The potential factors that link climate change and violence against women are not simple or linear. Recognizing the links requires investigation into the complex web of social and economic changes, and acculturation and health outcomes as result of climate change. This literature review examined research on climate change and violence against women. Selection criteria included English language articles and government documents published 2002 to the present accessible through PubMed using the following key words: violence, women, and climate change. Twenty-four documents met selection criteria and were included in this review. There is limited research examining the impact of climate changes on traditional lifestyle, economic stability and the role of family and the impact is has on violence experienced by women. Using a gendered approach to investigating and addressing climate changes may facilitate novel approaches to reducing violence against women. Further investigation of this issue may contribute to more complete surveillance of mental health issues, and social and economic stressors, and thus contribute to better health outcomes for women in Northern regions. Total Hg concentrations in freshwater fish at Adak Island, Aleutian Archipelago, Alaska Leah Kenney1*, Frank von Hippel1, Todd O’Hara2, James Willacker1 1 University of Alaska Anchorage, Department of Biological Sciences 2 University of Alaska Fairbanks *leahkenney@gmail.com The Aleutian Archipelago is an isolated arc of over 300 volcanic islands stretching 1600 km across the Bering Sea. Although remote, some Aleutian Islands were heavily impacted by military activities from World War II until the present and were exposed to anthropogenic contaminants, including mercury (Hg). Atmospheric and oceanic transport of Hg in the Aleutian Islands is a concern due to global atmospheric deposition and prevailing ocean currents. Recent research has documented high levels of Hg in marine fishes, seabirds, and marine mammals throughout the Aleutian Archipelago, but Hg contamination of freshwater ecosystems is poorly understood. Total Hg (THg) 13 concentrations were measured in threespine stickleback (Gasterosteus aculeatus) collected from eight freshwater lakes at Adak Island. Mean THg concentrations for whole-body homogenates for all lakes ranged from 0.314 to 0.560 mg/kg dry weight and differed between lakes. Stickleback collected from seabird associated lakes had significantly higher concentrations of THg compared to non-seabird lakes and lakes with formerly used defense sites. The δ13C and δ15N stable isotope ratios of stickleback collected from seabird lakes suggest a marine input of nutrients and contaminants. These findings indicate that seabirds are transporting marine derived Hg to freshwater lakes via guano and their tissues, and that the marine environment is a more important contributor of Hg contamination than the local military sites. Genomics underlying rapid phenotypic change in threespine stickleback populations from Prince William Sound and the Gulf of Alaska Emily Lescak1, 2*, William Cresko3, Frank von Hippel1 1University of Alaska Anchorage, Department of Biological Sciences 2University of Alaska Fairbanks, School of Fisheries and Ocean Science 3University of Oregon, Center for Ecology and Evolutionary Biology *elescak@alaska.edu The 1964 Great Alaska Earthquake was a colossal geologic event that uplifted several islands in Prince William Sound and the Gulf of Alaska, leading to the formation of freshwater ponds in close proximity to the coast. These ponds were subsequently colonized by oceanic threespine stickleback and, over the last few decades, these populations have already evolved freshwater phenotypes, such as reductions in body size and bony armor. I propose the use of high-resolution, broad coverage genotyping (using the recently developed restriction site-associated DNA genotyping approach) to study the genomics underlying rapid morphological change in these populations. Using singlenucleotide polymorphism data from the nuclear genome as well as mitochondrial DNA, I will determine population structure, look for evidence of introgression in freshwater and oceanic fish found within the same ponds, and identify candidate genomic regions and genes that may be involved in the initial phenotypic changes that evolve when oceanic fish colonize freshwater environments. 14 Distribution and ecology of zooplankton and juvenile salmon in the Copper River Plume Laurel McFadden1*, Rob Campbell2, Douglas Causey1, Jeffery Welker1, 3 1University of Alaska Anchorage, Department of Biological Sciences 2Prince William Sound Science Center 3 University of Alaska Anchorage, Environment and Natural Resources Institute * Lfmcf47@gmail.com The Copper River is the largest point-source of fresh water to the northern Gulf of Alaska, and is an important spawning environment for all five species of Pacific salmon (Oncorhynchus spp.). When entering the marine environment, salmon smolts are known to subsist on zooplankton, but their location and feeding patterns are little studied in the region. Preliminary sampling suggests juvenile salmon may use the highly turbid Copper River plume to evade predation and take advantage of concentrated zooplankton populations. The influence of the plume biochemistry and physical dynamics can create a non-homogeneous distribution of zooplankton that may correlate with smolt feeding behavior. Previous studies on salmon have proposed the critical size and period hypothesis, which suggests that after smolts enter the marine environment, they must achieve sufficient size within a certain time to survive their first marine winter. Fluctuations in marine conditions and changes in zooplankton concentrations and salmon behavior have been implicated as drivers of salmon year-class strength. This project will sample the Copper River plume for zooplankton and salmon smolts with bongo nets and trawling, and make concurrent measurements of salinity, temperature, and turbidity with an undulating towed vehicle to develop an oceanographic description of the plume. Further description of the trophic status of fish and zooplankton will be conducted via isotopic analysis, and salmon growth rates will be estimated from otolith measurements. We hypothesize that relationships between zooplankton and smolt concentrations will correlate with plume dynamics in a way that supports optimal juvenile salmon growth and survival. Life Meaning among Rural Alaska Native and American Indian college students Tonie Quaintance University of Alaska Joint PhD Program in Clinical-Community Psychology tmquaintance@alaska.edu Little research exists on how ethnic minority students subjectively define meaning in life; nor is there a well-accepted definition or explanatory model of meaning in life for rural Alaska Native/American Indian (AN/AI) students. This study gathers qualitative data that explores experiences, attitudes, and beliefs rural Native students transitioning to urban university describe as important in providing reasons and meaning in life. Insight into what meaning in life is to rural students can inform interventions that can facilitate adjustment skills and promote sense of purpose for students dealing with this transition. The participant criteria include: (a) 18-29 years of age; (b) full-or part-time student at either University of Alaska Fairbanks (UAF) or University of Alaska Anchorage (UAA); 15 (c) self-identifying as American Indian or Alaska Native and (d) from rural areas of Alaska. The qualitative interview asks participants to construct a narrative or “story” about experiences, attitudes, and beliefs they describe as important in providing meaning and reasons in life. Domains include where students find meaning, what meaning is to students, and how the process of adapting to urban university shaped their meaning in life. The study employs an exploratory, inductive research design in which ideas and themes emerge from the data (grounded theory). Emic concepts from the students on factors that provide meaning are identifying in the data (interviews). The emerging themes inform a culturally appropriate meaning in life measure that fits the experiences and issues rural Native students indentify in transitioning to urban university. This research broadens the understanding of young Native adults in rural Alaska who are making a transition to an urban university. The research findings have a potential to influence many education and social policies related to young adults in Alaska. The Role of Histone H2A in DNA MisMatch Repair David Robinson1*, Jocelyn Krebs1 1 University of Alaska Anchorage Department of Biological Sciences *suprdavr@gmail.com Chromatin is the combination of DNA and the proteins that help to package and organize it. The proteins are called histone proteins, and consist of two sets of four proteins called histones H2A, H2B, H3, and H4. These proteins contain a globular domain, around which DNA will wind to form a ‘beads-on-a-string’ configuration called nucleosomes, and a flexible tail region. This tail region protrudes from the globular domain, and will interact with the nucleosome, as well as mediate interactions between nucleosomes. Certain amino acids in the tail region are modifiable, meaning the cell can add a chemical group to the amino acid, changing its properties and the way in interacts with the DNA. These changes range from adding a group that carries a positive or negative charge, changing the way the basic amino acids interact with the negative DNA backbone, or chemical groups that are large and physically alter the DNA protein interactions. A number of modifications of histone H2A have been shown to be important for a number of different DNA repair processes in the past. Repair of UV damage, oxidative damage, chemically induced DNA double-strand breaks, but one area where the role of Histone H2A has not been thoroughly examined is the process of DNA Mismatch Repair. This is the system that helps a cell identify and correct areas where a cell has a single damaged DNA base, or a small group, and thus helps to ensure that during the DNA duplication process you don’t have an error copying the new strand of DNA, which would introduce a mutation. My project used a system to identify differences between the natural mutation rates of normal cells, and those with a mutated histone H2A, and identify any cells that may have an increased rate of spontaneous mutation due to a defect in Mismatch Repair. 16 Measuring resilience among Somali refugees living in the United States Rebecca Volino Robinson University of Alaska Joint PhD Program in Clinical-Community Psychology rebeccarobinson11@gmail.com Somalia is a country rife with conflict. The Somali Civil War, which began the mass migration of hundreds of thousands of Somali men, women, and children out of Somalia, began in 1991 and continues today. Most, if not all, Somali refugees have witnessed horrific acts of violence and many are victims of physical and sexual violence themselves. As a result, Somali refugees are disproportionately affected by mental health disorders and social problems, such as Posttraumatic Stress Disorder, depression, poverty, difficulty with school, and gang violence. The literature clearly represents the problems faced by Somali refugees, pre- and post-migration. However, despite the fact that over 80,000 Somali refugees have survived the civil war, fled Somalia, obtained refugee status, and resettled in the United States, the literature lacks any empirical data on Somali refugee resilience. A likely reason why the refugee literature in general, and the Somali refugee literature in specific, lacks data on resilience is the lack of culturally relative measures of resilience. Protective factors and positive psychological outcomes vary depending on the context of the population studied. Based on the literature reviewed, the best measure of adult resilience was developed in the Western world, based on Western ideology and worldview. The purpose of this presentation is to describe my dissertation research, which seeks to develop a measure of Somali refugee resilience from the ground up. This scale will be the first step to identifying Somali refugee strengths and protective factors. Barriers and facilitators to self-care in graduate students Kari Sandberg1* and Rebecca Volino Robinson1 1 University of Alaska Anchorage, PhD Program in Clinical-Community Psychology *karinsandberg81@gmail.com Graduate students are notoriously under high levels of stress from immense amounts of work and study. It is common for such students to live life somewhat out of balance and to neglect self-care as a result of the immense amount of personal resources being dedicated to work and academic obligations. Self-care practices have been shown to help prevent burnout. Preventing burnout among students and young professionals is essential to successful completion of academic requirements, early career building, and overall health. The purpose of our study was to evaluate barriers and facilitators to selfcare among graduate students at the University of Alaska Anchorage (UAA). We conducted a series of focus groups with graduate students attending UAA in order to understand the barriers and facilitators to self-care and to inform students and administrators of programs how best to prevent burnout with this population. The purpose of this presentation is to disseminate preliminary findings from these data and discuss future directions for self-care among graduate students attending UAA. 17 A survey of blood parasites from southern Alaskan brown and black bears using microscopic and molecular techniques Becky Sawyer University of Alaska Anchorage, Department of Biological Sciences rebecca.sawyer@sbcglobal.net Few studies have been performed to catalog the microfaunal parasites carried by the three species of bears found in America. This is especially true in Alaska, where the most comprehensive studies of parasites were done over five decades ago. It is important to establish a baseline for parasite load, particularly in light of studies that have shown that parasites can change geographic distribution in response to climate change. Using blood smears and tissue samples collected by ADF&G, brown and black bears were screened for common blood parasites using both microscopy and PCR. These parasites include Babesia sp., Trypanosoma sp., Eimeria sp., Toxoplasma gondii and Hepatozoon sp. The data collected will be compared to genetic features of the host bears, specifically the major histocompatibility complex, to determine if there is a correlation between genetic identity and parasite load. Ultimately, this study will help to elucidate how parasites help to maintenance of variation within the MHC over evolutionary time. Impacts of Melilotus alba on pollinator services, diversity and abundance in the boreal forest of interior Alaska Laura Schneller University of Alaska Anchorage, Department of Biological Sciences lcschneller@alaska.edu Pollination is a critical ecosystem service that often is required or greatly increases fruit set for angiosperms. Pollination services can be disrupted by non-native plant species that are highly rewarding. This can impact plant-pollinator networks, visitation rates, and fruit and seed production in co-occurring native plants. Boreal Alaska is currently experiencing an invasion of a highly rewarding plant, Melilotus alba that is spreading along floodplains, roadsides and burn areas in Alaska. The questions tested in this study are 1. whether pollinator abundance and richness is different in communities with and without M. alba, and 2. whether per flower visitation rates change in communities with and without M. alba. I sampled pollinator diversity and abundance and recorded visitation rates in 24 sites in interior Alaska, 13 with and 11 without M. alba. Initial results indicate an increase in pollinator abundance and richness and a decrease in pollinator visitation to native plants at sites with M. alba. 18 An evaluation of the current methods of monitoring annual production of Midcontinent Greater White-fronted Geese (Anser albifrons) that breed in Interior and Northwest Alaska Wade Schock University of Alaska Anchorage, Department of Biological Sciences wschock@uaa.alaska.edu The population of midcontinent greater white-fronted geese has fluctuated significantly over the last ten years. One subpopulation, white-fronted geese from interior and northwest Alaska, has drawn specific attention because of lower annual survival rates. Wildlife agencies responsible for managing waterfowl harvest and habitat need better methods of assessing population status and identifying factors that affect population dynamics of these geese. My aim is to evaluate two assumptions associated with current methods that measure annual production of white-fronted geese that breed in interior and northwest Alaska. First, I will test the assumption that current techniques to monitor production at Delta Junction, Alaska with age-ratio estimation are not biased by timing of surveys. To test this assumption, I will measure changes in the ratio of adult to juvenile geese in Delta Junction, Alaska. Specifically, I will conduct group counts daily during the migration period and use a regression analysis to assess changes in age ratios over time during the migration season. Second, I will test the assumption that whitefronted geese migrating through the Delta Junction area in the fall are representative of the entire interior and northwest Alaska subpopulation. To do this, I will compare isotopic signatures of sample feathers collected from white-fronted geese migrating through the Delta Junction area with reference sample feathers collected from whitefronted geese on their breeding areas throughout interior and northwest Alaska. I will obtain sample feathers from birds killed by hunters and destructive sampling. I will then use mass spectrometry to measure and analyze stable isotopes of hydrogen, carbon, oxygen, nitrogen and sulfur. A principal components analysis will be used to test whether there are distinguishable isotopic patterns in the feather samples collected. The results of this research will enable waterfowl biologists to better assess the status and identify factors that affect the population dynamics of white-fronted geese from interior and northwest Alaska so that they can be managed most effectively. Partitioning ecosystem respiration in arctic tussock tundra to more accurately forecast changes in the carbon cycle Aliza Segal1*, Patrick Sullivan1,2 1 University of Alaska Anchorage, Department of Biological Sciences 2 Environment and Natural Resources Institute, University of Alaska Anchorage * segaliza@gmail.com Many arctic ecosystems that have historically been strong carbon sinks are becoming carbon sources. Although ecosystem respiration is the largest carbon flux out of ecosystems, our ability to model respiration lags considerably behind our ability to model photosynthesis in this ecosystem. Additionally, understanding these fluxes is 19 especially important for an ecosystem which appears to be experiencing the greatest climate warming and also contains large stores of soil carbon. Partitioning respiration into its component fluxes is a critical first step towards improving our ability to model changes in respiration. I aim to partition and model each component, both above and belowground, into autotrophic and heterotrophic constituents to gain a better understanding of the biotic and abiotic factors controlling respiration. Partitioning belowground constituents has proven to be challenging in most ecosystems. Therefore, I will use three methods to accurately estimate root respiration and bulk soil microbial respiration. The first involves measuring excised root respiration and scaling these values to the ecosystem level. The second is an herbicide treatment that eliminates respiration of all autotrophic species, and the third is an isotopic 13CO2 pulse-chase approach that results in minimal disturbance of belowground processes. We will measure leaf, wood, and mosses separately, and scale these values to the ecosystem level as well. I expect belowground respiration to be a large percent of total ecosystem respiration. This hypothesis is contrary to the currently accepted model which assumes that minimal amounts of CO2 originate from the soil. These models will produce more accurate predictions of ecosystem respiration in future climatic conditions. Behavioral health aide manual ethics Matthew Sena University of Alaska Anchorage, Department of Clinical-Community Psychology Mattsena2003@yahoo.com This presentation will describe the process and outcome of a community based participatory project to develop Behavioral Health Aide Manual (BHAM) ethics chapter. The BHAM is a new practice manual in press, authored by a state-wide committee of rural indigenous behavioral health aides, supervisors and mental health practitioners, coordinated by the Alaska Native Tribal Health Consortium. The ethics chapter took approximately three months to develop, blending traditional Alaska Native values with clinical behavioral health ethics. Qualitative and quantitative data will be presented, summarizing community feedback on its cultural responsiveness, as well as the final chapter product. 20 CO2 exchanges in High Arctic ecosystems: responses to multiple levels of warming and increases in summer precipitation, NW Greenland Elizabeth Sharp1*, Patrick Sullivan1,2, Claudia Czimczik3, Jeffrey Welker1,2 1 University of Alaska Anchorage, Department of Biological Sciences 2 Environment and Natural Resources Institute, University of Alaska Anchorage 3 University of California Irvine, Department of Earth System Sciences * esharp0369@gmail.com Greenland’s climate is changing at rates unprecedented across the Arctic and these are especially acute in the NW region of the island. For the past 9 years we have been examining how experimental low and high warming, with and without added summer water will alter CO2 exchanges and soil and vegetation traits as part of the Biocomplexity and International Tundra Experiment Programs. We found that low levels of warming with no water addition (T1) resulted in a 60% (2009) and 110% (2010) increase in net CO2 uptake. High levels of warming with no water addition (T2) resulted in a 30% (2009) and 60% (2010) reduction in net CO2 uptake. High warming and added summer water (T2W) appear to stimulate net CO2 fixation the most, increasing net CO2 sequestration by 140% in 2009 and 210% in 2010. These differences are caused by the strong co-limitation of gross ecosystem photosynthesis by temperature and water. The higher rates of net CO2 uptake are due primarily to higher rates of gross ecosystem production (GEP) as opposed to lower rates of ecosystem respiration (ER). Our findings are consistent between 2009 and 2010 and indicate that the combination of warmer summers plus increases in rain will have the largest effect on CO2 exchange and thus carbon cycling in these extreme habitats which in turn may affect vegetation cover and surface albedo. Recent phenotypic and molecular evolution of trophic polymorphism in a species pair of kokanee (Oncorhynchus nerka) in southwest Alaska Kyle Shedd University of Alaska Anchorage, Department of Biological Sciences krshedd@alaska.edu Several morphologically distinct species pairs occur across many freshwater fish taxa as a result of differences in feeding ecology and trophic life history. Such distinctions may arise from divergent selection due to food competition and niche adaptation, providing an example of ecological speciation. Sockeye salmon (Oncorhynchus nerka) have diverged into anadromous and nonanadromous resident populations (kokanee) in many lakes throughout western North America. However, trophic polymorphism within kokanee populations has not previously been documented. Kokanee are thought to be constrained to a planktivorous diet due to competition from other limnetic predators. In the present study, I will examine a previously documented naturally occurring species pair of kokanee in Lake Jo-Jo in southwest Alaska that appears to have arisen within the past 200-800 years. Both suspected morphotypes appear to have adopted distinct, non-planktivorous diets; one piscivore morph specializes on 21 threespine stickleback, and one non-piscivore morph appears to specialize on leeches. Piscivore and non-piscivore morphotypes will be defined using both stomach content analysis and carbon and nitrogen stable isotope analysis. Once separated, I will analyze the two populations for differences in head, body, and gill raker morphology using geometric-morphometrics and meristic parameters. Additionally, life history parameters such as population size, age class structure, and fecundity will be assessed for the two ecotypes. Finally, genetic analysis using fourteen unique tetranucleotide microsatellites will demonstrate whether any potential divergence in diet, morphology, and life history among the morphotypes might have led to and be enforced by genetic differentiation. Such genetic divergence could be indicative of reproductive isolation and the initial stages of ecological speciation. Stable isotope (δ13C, δ15N) analysis and satellite telemetry depict the complexity of Gray Wolf (Canis lupus) diets in Southwest Alaska Ashley Stanek1*, Dominique Watts2, Brian Cohn1, Page Spencer3, Buck Mangipane3, Jeffery Welker1 1 Environment and Natural Resources Institute, University of Alaska Anchorage, Anchorage, AK 99508 2 U.S. Fish and Wildlife Service, Alaska Peninsula and Becharof National Wildlife Refuges, King Salmon, AK 3 National Park Service, Lake Clark National Park and Preserve, Port Alsworth, AK 99653 *ashleystanek@gmail.com Throughout Alaska, gray wolves (Canis lupus) are a top predator of large ungulates. While they primarily rely on ungulates such as moose (Alces alces) and caribou (Rangifer tarandus), they are opportunistic feeders and also use alternative resources. The variation and supplemental protein sources in wolf diet has not been studied extensively on live animals currently using the landscape. With large seasonal influxes of Pacific salmon (Oncorhynchus sp.) into Alaska, terrestrial carnivore use of marine species is of particular interest. Using stable isotope (δ13C, δ15N) analysis of wolf guard hair and blood, this study aims to determine the proportion of marine derived nutrients (MDN) in the diet of wolf packs in southwest Alaska. Satellite telemetry from the animals sampled facilitates quantification of landscape use patterns in correspondence with isotopic traits. Wolf pack territories in and around Lake Clark National Park appear to vary in spatial extent and in availability of MDN, such as salmon. Initial analysis shows that two packs with smaller home ranges, centrally located around areas with greater salmon availability, have enriched δ15N values compared to packs that have larger home ranges not centralized around salmon spawning waters. This pattern of isotopic enrichment is found in red blood cells, blood serum and hair, representing diets over different time scales. The enrichment in both blood and hair indicates a sustained use of MDN over the previous six to nine months. In the Lake Clark region, packs with smaller home ranges salmon may be a substantial component of their diet. In contrast, packs with larger home ranges and less access to salmon have stable isotope values representative of a terrestrial diet. 22 To reproduce or not: Nutrition-hormonal influence on reproductive decisions in seasonally synchronous breeders Cory Stantorf University of Alaska Anchorage, Department of Biological Sciences corystantorf@gmail.com Changes in forage quality and quantity can affect body condition of herbivores which, in turn, has consequences for reproductive fitness. To date, few if any studies have addressed how large herbivores integrate nutritional signals resulting in reproductive decisions. Understanding the link between nutritional status and the hormones, leptin and ghrelin in moose may provide a significant improvement in our ability to assess a bottom up mechanism that modulates an animal’s productivity and population potential. Furthermore, it will better enable us to understand the current status and potential of moose populations, regardless of the nature of the limitations present. The effects of hibernation on the gut microbial community of the Arctic Ground Squirrel Tim Stevenson University of Alaska Anchorage, Department of Biological Sciences tim.stevenson83@gmail.com Hibernation is both a behavioral and physiological adaptation employed by endotherms to survive periods of reduced food availability. It is characterized by protracted episodes of inactivity and profoundly decreased rates of metabolism and body temperature. The arctic ground squirrel (Urocitellus parryii) is the northern most hibernator in North America and, as such, has evolved the most extreme hibernation phenotype, exhibiting the greatest depth, duration and energy savings of hibernation of any species studied. The impact of hibernation on the gut microbial flora has been little studied across hibernating species, and of the few studies, all but one was limited to culture dependent techniques. Host organisms and gut microbes share a mutualistic relationship. The host depends on microbes for digestion and energy extraction, while microbes depend on their host for substrate and an environment in which to live. Despite the positive benefits of the relationship, gut microbes and the host can influence each other negatively. For example, gut microbes have been implicated in disease, such as, irritable bowel disease and obesity, although the relationships are not fully understood. I propose to investigate the effects of hibernation on the microbial flora of arctic ground squirrels using culture-independent methods. Diversity, composition, enumeration, and metabolic short chain fatty acid production of the gut microbial community will be measured at various time-points and phases of the hibernation season: pre-hibernation fattening, late torpor, late arousal, and post-emergence. Hibernators have been used extensively as models for studies of human diseases such as diabetes, stroke, ischemia and reperfusion and obesity. The extreme physiology of the arctic ground squirrel 23 suggests that this species could serve as an excellent model for studies of gut microbialhost interactions, gut microbe-related diseases, and physiology of the gut. Testing the conservation of the mammalian WINAC and B-WICH ATP-dependent remodeling complexes in Xenopus laevis Nicole Stopa1*, Jocelyn Krebs1 1 University of Alaska Anchorage, Department of Biological Sciences *nstopa@gmail.com The DNA inside our cells is in a highly compacted form termed chromatin. ATPdependent chromatin remodelers utilize the energy of ATP to rearrange chromatin and are necessary for regulation of gene expression, especially during development. As such, dysfunction of ATP-dependent chromatin remodelers has been linked to a number of developmental diseases. One of these diseases is Williams-Beuren Syndrome, in which a section of chromosome 7 containing approximately 26 genes is deleted. One of these genes, Williams Syndrome Transcription Factor (WSTF), encodes a protein that is a component of at least three biochemically identified ATP-dependent chromatin remodeling complexes in humans: WICH, B-WICH and WINAC. In Xenopus laevis, however, only WICH has been shown to be present. ATP-dependent chromatin remodeling complexes are highly conserved, with many specific complexes occurring in yeast, Drosophila, Xenopus and humans. Therefore, it would be expected that the WINAC and B-WICH complexes also exist in Xenopus, especially since WSTF is present and it forms the WICH complex. WINAC is necessary to mediate binding of the vitamin D receptor (VDR) to VDR-responsive gene promoters. The evidence for the existence of WINAC is especially strong given the presence of VDR in Xenopus. I propose to test interaction of WSTF with the WINAC-specific component VDR and the B-WICH-specific component SAP155 by doing coimmunoprecipitation assays in Xenopus embryo extracts. If these assays indicate VDR and SAP155 stably interact with WSTF in vivo it would be indicative of the presence of the WINAC and B-WICH complexes in Xenopus. This finding will allow more in depth studies of the molecular pathways that lead to many of the developmental abnormalities seen in William-Beuren Syndrome, as well as fundamental gene expression and molecular interactions needed for normal development. If these ATP-dependent chromatin remodeling complexes are not present in Xenopus, a potentially important difference between mammalian and amphibian development will be discovered. 24 Habitat-specific foraging drives mercury concentrations in benthic and limnetic morphotypes of stickleback in Benka Lake, Alaska James Willacker1,2 *, Frank von Hippel1, Kerri Ackerly3, and Todd O’Hara2 1 University of Alaska Anchorage, Department of Biological Sciences 2 University of Alaska Fairbanks, Department of Biology and Wildlife and Institute of Arctic Biology 3 Adelphi University, Department of Biology *jjwillac@gmail.com Mercury (Hg) is a widespread environmental contaminant known for the neurotoxicity of its methylated form, which biomagnifies in aquatic food webs. The concentration and biomagnification of Hg is known to vary among species utilizing different food webs (benthic versus limnetic) within and between systems. We used stable isotopes of carbon and nitrogen to determine if similar differences in total Hg (THg) concentration and biomagnification could be observed in threespine stickleback fish (Gasterosteus aculeatus) from Benka Lake Alaska, a population that demonstrates both benthic and limnetic morphotypes. The average THg concentration in the limnetic morphotype was nearly twice that of the benthic morphotype and THg concentration was significantly correlated with the proportion of benthic carbon in the diet of individual fish. Limnetic fish had a higher average trophic position than benthic fish and calculated biomagnification factors were greater in the limnetic food web. Additionally, strong sex effects were observed with female fish of each morphotype having lower THg concentrations than males. These results indicate that trophic ecology and sex are both important determinates of Hg contamination even within a single species and lake, and may have important implications for Hg levels in higher trophic levels. Trauma in schizophrenia disorders: Asking the question Ann Yates1* 1 University of Alaska Anchorage, PhD Program in Clinical-Community Psychology *annkyates@gmail.com I am creating a research study as part of my project/thesis for my Master’s of Science in Nursing/Psychiatric Nurse Practitioner program. This qualitative study asks the following question: what do people with schizophrenia disorders and a history of trauma experience when asked about past trauma? In research studies, 29-46% of people with schizophrenia disorders have a history of significant trauma, and yet by chart review only 3-4% have trauma documented (Muenzenmaier, 2005; Lommen & Restifo, 2009). Somehow the process of asking questions about trauma is not working well; research indicates healthcare providers have concerns about the validity of responses and the possibly negative effect questions may have on clients (Mueser, Bolton, & Rosenberg, 2007). In addition, trauma screening tools that do not involve an open interview may not be adequate for those with schizophrenia disorders as this population has significant difficulties with trust and feelings of shame or guilt (Tucker, 2002). Trauma greatly complicates and worsens the prognosis for this population and leads to a poor quality of 25 life (Duke, Allen, Ross, Strauss, & Schwartz, 2010). My project seeks to further understand the process of asking about trauma, and therefore diagnosing trauma, in those with schizophrenia disorders. In this process the clients’ point of view is essential and yet studies have not specifically focused on this. Therefore, my study revolves around a series of interviews with people with schizophrenia disorders and a history of trauma. The more information that is gathered and explored, the more diagnosis and treatment of schizophrenia and trauma can improve. The more diagnosis and treatment improve, the better this population can manage their mental and physical health, and flourish in the community. 26 Plenary Speakers Paula Donson: Associate Vice President for Academic Affairs for the University of Alaska System Paula assists with implementation of the university’s Academic Master Plan, is involved in Strategic Planning, and oversees the e-learning program. She is adjunct faculty at the College of Business and Public Policy and College of Engineering’s Project Management Program (which she helped launch). She has been involved in several Alaskan businesses, including the Alaska Railroad Corporation, Mactel Incorporated, Alaska Seafood International, and Alaska USA Federal Credit Union. Stephanie McAfee: Climate change scientist with The Wilderness Society in Anchorage Stephanie works on questions about how to use climate change projections for land management planning. Making her way steadily north, she moved to Anchorage in July after a PhD at the University of Arizona in Tucson and a year as a National Research Council postdoc at the NOAA Earth System Research Lab in Boulder. She will be presenting on: The Life History of a Climate Projection Projecting climate change is often thought to be the solely domain of physicists and computer programmers. However, people with a wide range of specialties are involved in developing, creating, and using climate projections. As we move toward using climate projections to shape policy on a number of fronts, it is important to understand the complete process of developing a climate projection from the socio-economically based input scenarios, to the mechanics of running a state-of-the-art climate model to the many ways in which model output is used. David Yesner: Associate Dean of the UAA Graduate School David’s primary research interests are environmental archaeology, ecological anthropology, and hunting and gathering societies. His research has taken him all over North America, as well as Cyprus, but his main interests lie in circumpolar areas, such as Alaska, Russia, and southern South America. He has recently participated in a number of archaeological excavations in these three regions. 27