seven (7) days. Hemolyzed specimens should not be used
as
erythrocytes contain fifteen times the AST (SGOT) activity
in serum.4
Safety precautions
For in vitro diagnostic use only.
Do not pipette by mouth.
Exercise the normal precautions required for handling
laboratory reagents.
QUALITY CONTROL
Normal and abnormal control sera of known concentrations
of AST (SGOT) should be analyzed routinely with each
group of unknown samples
AST (GOT)
Colorimetric End point
*Reaction principle
The colorimetric determination of AST is based on the
following reaction:
L-Aspartate+ 2-oxoglutarate
Glutamate
Oxaloacetate+ L-
Wavelength
Optical path
Incubation temperature
Measurement against
546 nm (530 - 550 nm)
1 cm
37°C
Reagent blank
Pipette into test tubes
Blank
Specimen
-----
Specimen
0.1 ml
*Reagents composition, Contents and Safety warnings
R1
R2
Additional
Reagent
Phosphate buffer pH
7.4
L-Aspartate
2-Oxoglutarate
2,4dinitrophenylhydrazine
Biomed-Sodium
Hydroxide
Storage and Stability of Reagents
Store at +2-8oC .
Stable until the expiration date reported upon the package.
After the unsealing and the taking of the reagent , it is
advised to close up the bottle immediately in order to avoid
evaporation , direct light exposure and bacterial
contamination
Reagent Preparation
All reagents are ready for use.
Sample
This assay is intended for use with human serum. Reports
indicate
that AST (SGOT) in serum remains stable at 4°C for a
minimum of
Obtain the activity of GOT in the serum from the table
Absorbance
U/l
Absorbance
U/l
0.020
7
0.100
36
0.030
10
0.040
13
0.120
47
0.050
16
0.130
52
0.060
19
0.140
59
0.070
23
0.150
67
0.080
27
0.160
76
0.090
31
0.170
89
41
*Procedure
Oxaloacetate formed reacts with 2,4dinitrophenylhydrazine to yield a colored hydrazone that
can be measured at 546 nm (530-550).
100
mmol/l
100
mmol/l
4.0
mmol/l
2.0
mmol/l
0.4
mol/l
*Calculation
Reagent 1
0.5 ml
Dist. Water
0.1 ml
0.5 ml
-----
Mix, incubate for exactly 30 minutes at 37°C.
Reagent 2
0.5 ml
0.5 ml
Mix, allow standing for exactly 20 minutes at 20-25°C.
NaOH (0.4 mol/l)
5.0 ml
5.0 ml
Bibliography
F. Schiele: Additional information regarding the
recommended method for determining the catalytic
concentration of alanine aminotransferase in human serum
at 30 ° C.: Ann. Biol. Clin. 40, 87 (198 2)
For in vitro diagnostic use only.
The following symbols are used on labels
For in vitro diagnostic use
Mix, read the absorbance of specimen (Specimen) against the
reagent blank after 5 minutes. The color intensity is stable for 1
hour.
Use by (last day of the month)
Temperature limitation
Batch Code
*EXPECTED VALUES
Serum
Up to 12 U/l