SODIUM
Colorimetric Method
Principle
The Present method is based on reaction of sodium
with a selective chromogen producing a
chromophore whose absorbance varies directly at
the concentration of sodium in test.
Reagent composition, Contents and Safety warnings
standard Sodium
Reagent 1
150 mEq/l
Reagent 2
Color reagent
Pipetting in tubes:
BLANK
Reagent
1ml
(R2)
Distilled
10 µL
water
Standard
Sample
STANDARD
1ml
SAMPLE
1ml
10 µL
Mix, incubate for 5 min at room temperature (+1525°C.) Read the absorbance of standard and sample
tubes.
Volumes can be proportionally modified.
This methodology describes the manual procedure
to use the kit.
For automated procedure, ask for specific
application.
Calculation
(A) Sample
Store all reagents at +2-8oC the reagents are stable
until the expiration date as indicated on the label.
Sample
Fresh serum free of haemolysis or Plasma with Lithium heparin
Note: Do not use EDTA
Safety precautions
For in vitro diagnostic use only.
Do not pipette by mouth.
Exercise the normal precautions required for handling
laboratory reagents.
Procedure
Wavelength:
Optical path:
Temperature:
Reading:
Assay type:
623nm (620-640)
1 cm light path
+25/30/37°C.
Against reagent blank
End Point
Reference values
Serum or Plasma:
Quality control
Control sera are recommended to monitor the performance
of manual and automated assay procedures.
Note
Use only new or perfectly cleaned glassware and tips.
Free from metallic lons or oxidants.
Bibliography
1. Kumar et Al : Clin. Chem. 34/9, 1709 - 1712 (1988)
2. Berry et Al : Clin. Chem. 34/9, 2295 . 2298 (1988)
For in vitro diagnostic use only.
The following symbols are used on labels
135 - 150 mEq/l
For in vitro diagnostic use
Use by (last day of the month)
These values should only be used as a guideline.
Each laboratory should establish its Normal Reference
Range.
Performance Characteristics
A. LINEARITY LIMIT
The reaction is linear in the range 80 - 180 mmol/l
For higher value dilute sample 1:2 with deionized water,
repeat the test and multiply the result by 2
B. DETECTION LIMIT
Values less than 80 mmol/l give - reproducible results
C. INTRA ASSAY PRECISION
Mean (mmol/l)
Level 1
120
Level 2
160
CV%
3.90
4.72
F. INTERFERENCES
Triglycerides till to 2500 mg/dl do not interfere
Bilirubin till to 27 mg/dl does not interfere
× 150
(A) Standard
Storage and Stability of Reagents
SD
4.80
7.31
E. ACCURACY
Comparation between this method and the determination
by Flame Photometry shows a correlating factor r = 0.985
10 µL
Sodium mEq/l =
D. INTER ASSAY PRECISION
Mean (mmol/l)
Level 1
123
Level 2
155
SD
2.48
4.59
CV %
2.06
2.86
Temperature limitation
Batch Code
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