http://ki.mit.edu/sbc/microscopy/instrumentation
Instrumentation
Fluorescence and Bright Field Microscopy
 Applied Precision DeltaVision Spectris Imaging system, best for
live/fixed thin specimens
o Inverted Olympus X71microscope with XYZ nano-motion stage,
environmental chamber, Mercury lamp illumination and
o Softworx deconvolution software, see specifications.
 Applied Precision DeltaVision Microscope with Ultimate Focus and
TIRF module, best for live cell imaging, photokinetics experiments and
TIRF imaging
o Inverted Olympus X71 microscopes with XYZ nano-motion stage,
environmental chamber, Solid State illumination module, four (4)
lasers, Ultimate Focus, TIRF module, and
o Softworx deconvolution software, see specifications
 Applied Precision DeltaVision-OMX – Super-Resolution Microscope,
best for thin specimens
o Experimental microscopes (OMX) with XYZ nano-motion stage,
Structured Illumination, lasers, three cameras, heated stage, and
o Softworx processing software, see specifications.
 Nikon Laser Spinning-disk Confocal Microscope with TIRF module, best
for live imaging/thick specimens and for live TIRF imaging
o Nikon inverted microscope with XYZ stage, with lateral arm
Yokogawa spinning disk and back illumination TIRF module,
environmental chamber, laser / Mercury lamp illumination and
MetaMorph software, see specifications.
 FV1000 Olympus Multiphoton Confocal Microscope, best for imaging
live/fixed thick samples
o Olympus inverted IX81 microscope with heated & CO2 stage
incubator, MaiTai laser, photomultiplier detector -2 channels, and
o Olympus software, see specifications.
 Zeiss microscope, good for fixed thin samples and histology
o Zeiss Axioplan II upright microscope with monochrome and color
cameras, Mercury lamp illumination, and OpenLab / Volocity
software, see specifications.
Specialty Microscopes
 Olympus microscope specialized for Spectral Karyotyping (SKY) and
Fluorescence In Situ Hybridization (FISH).
o Olympus X70 upright microscope with SpectraCube interferometer,
Xenon illumination lamp, and Applied Spectral software, see
specifications.
 Arcturus Laser Capture Microdissection system ideal for precise –under
microscope - collection of tissue cells for DNA/RNA analysis.
o Compact system incorporating an inverted microscope for direct
visualization of the cells of interest, an IR diode laser and a UV laser;
see specifications, see specifications.
Image processing workstations/computers
 Linux workstation with Softworx software for image processing &
deconvolution of the data generated on the DeltaVision microscopes.
 Mac Power G5 computer with Volocity/OpenLab software for image
processing and for facilitating data transfer from all the Unix based imaging
systems.
 PC –dual core with Imaris, MicroView and Live software for image
processing and for facilitating data transfer from and to Window based
imaging systems.
Sample preparation
 Edwards Vacuum Deposition System for metal and carbon coating of
electron microscopy samples.
 Tousimis Samdri-795 Critical Point Drier for sample preparation for
electron microscopy.