1 ONLINE SUPPLEMENTAL FILE 2 APPENDIX 1 – PROCEDURE FOR WETLAND LAND COVER ASSESSMENT 3 4 Riparian buffers play an important role in improving the overall water quality of wetlands 5 by intercepting and filtering surface run-off containing nutrients and sediments (Skagen 6 et al 2008). Two scores are calculated, first a riparian score for a 50 m buffer width, 7 starting from the wetland itself and representative of the ability of its 50 m buffer strip to 8 filtrate contaminants from the upland zone. This 50 m value is chosen as the The 9 Planning and Development Act 2004 says that no construction must be done at 15 m 10 buffer zone restriction with a further 35 m control zone. So, it is assumed that there will 11 be mostly some form of natural vegetation existing within the 50 m buffer strip. The 12 second score is calculated for a buffer width of 950 m, starting from the outer edge of the 13 50 m buffer (Note that the distance of each buffer is bounded by the watershed of the 14 wetland). This means that if a wetland has a watershed (i.e., an upslope drainage area) of 15 only 100 m2 and maximum length 15 m, then the buffer cannot go past this maximum 15 16 m length and in area, cannot exceed 100 m2. The watershed, in this case, is the limit to 17 the surface water flow to the wetland. Any land cover outside a wetland’s watershed is 18 thus assumed to not affect or contribute to surface water flow to the wetland. The 19 drainage basins, flow direction and flow accumulations were obtained from Nigel and 20 Rughooputh (2010b). 21 22 1 23 Assessing land cover types and their threats to wetlands 24 A land cover map of Nigel and Rughooputh (2010a) lists 11 land cover types obtained by 25 partly digitizing the LU maps and its updated versions, coupled with supervised image 26 classification on a SPOT 10 m pan sharpened multi-resolution image of 1994. The land 27 cover map is in raster format with 10 m cell size. The 11 land cover (LC) types were 28 assigned risk factors, indicative of the potential for a particular LC to either improve or 29 impact negatively on the overall condition of a wetland and ranges from 0 (no impact) 30 representing the lowest risk, to 5 (very high impact) or equivalent to highest risk. The 11 31 land cover types and their associated risk factors for the 50 m buffer width and 950 m 32 buffer width are: sugarcane (5 for 50 m, 4 for 950 m); sparse vegetation (2, 2); scrub (3, 33 2); barren land (4, 3); food crops (5, 4); tea (4, 4); forest (1, 1); urban area (5,5). The 34 remaining land cover types which are wetlands, water bodies and sand are given a risk 35 factor of 0 (no impact) as they are assumed to be of no threat to the wetlands themselves. 36 37 Generating Wetland Buffer Zones 38 The ArcGIS™ software, ArcInfo™(9.3) was used to create wetland buffer zones using as 39 main inputs 1) a GIS data layer containing the 14 wetland polygons and 2) the land cover 40 map of Nigel and Rughooputh (2010a) at 10 m ground pixel size. In GIS, buffer widths 41 can be automatically generated using the buffer analysis tool. After selecting the wetland 42 polygon of interest from the wetland layer map, two buffer widths, one of 50 m, labelled 43 riparian buffer zone and a second buffer width of 950 m (starting from the 50 m buffer 44 zone) labelled upland buffer zone, were created for each polygon. A total buffer width of 2 45 1 km was thus generated around each wetland. Adjustments to the 950 m buffer zone 46 were needed in order to account for watershed boundary and upslope contributing areas 47 only. As an example, La Prairie wetland’s buffer zones were trim to keep only upslope 48 contributing areas that are inside the wetlands’ watershed boundary (Fig. 2). The river 49 basins and watersheds of the island, which give the equivalent watershed perimeters for 50 the studied wetlands, were obtained from Nigel and Rughooputh (2010a). 51 52 53 Fig. 2 Buffer zones for La Prairie wetland 3 54 55 Analysis of slope 56 We also aim to determine the slope gradient of the buffer zones to assess their 57 susceptibility to erosion, which affects wetland condition through surface run-off. Nigel 58 and Rughooputh (2010a) found that nearly 31% of the Island’s surface area has slopes > 59 8% which by soil erosion standards, are areas highly susceptible to erosion. The slope 60 map used in this paper for slope analysis is at 25 m cell resolution, was produced in 61 ArcGIS™ software from a 25X25 m Digital Elevation Model (DEM). The 25 m DEM in 62 turn was produced in ArcGIS™ software with the topogrid tool, which uses the 63 ANUDEM programme of Hutchinson (1989). Input elevation data for the DEM creation 64 were 10 m interval contours from MHL (2005). The slope layers (in percent) for the 50 m 65 and 950 m buffer zones were extracted for each 14 wetland using the ‘Extraction by 66 mask’ spatial analyst tool in ArcInfo™(9.3), where in this case, the mask corresponds to 67 the buffer zone being used. Mean slope and standard deviation for each slope layer was 68 then obtained by enquiring after the ‘slope layer statistics’. Slope statistics are 69 automatically calculated by the software and include mean, standard deviation, maximum 70 and minimum values of the extracted slope. 71 72 Calculating percentage land cover 73 The method used was to first extract the land cover pattern corresponding to a particular 74 buffer zone using the ‘Extraction by Mask’ spatial analyst tool in ArcInfo™ 9.3. This 75 step generates a land cover layer which was saved as a layer file. The GIS software 4 76 automatically generates an attribute table for each land cover layer created which consists 77 of the number of ‘counts’ of cells (pixels) for each land cover feature, where one count is 78 equivalent to one pixel size of 10X10 m (or 100 m2). The attribute table was then 79 exported to a Microsoft Excel Sheet for calculation of percentage land cover within each 80 buffer. To calculate the percentage of a land cover class within a particular buffer zone, 81 for example sugarcane, the total number of counts for the buffer zone layer was first 82 computed. The number of counts for sugarcane was then divided by the total number of 83 counts to obtain percentage sugarcane. This procedure was carried twice for each 14 84 wetlands, one for the upland buffer zone and the other for the riparian buffer zone. 85 86 Final computation of Wetland Land Cover Assessment (WLCA) 87 After obtaining all these information on land cover types, the following steps are carried 88 out to calculate the WLCI: 89 1. Assign risk factors (0–5) to each 11 land cover types 90 2. Calculate the percentage of each 11 land cover types (sugarcane, scrub, forest, 91 urban area etc.) in riparian buffer zone for wetland of interest 92 3. 93 corresponding risk factor. This step is repeated for each 11 land cover types 94 4. 95 score (RIPSCORE) 96 5. 97 upland buffer score (UPLSCORE) A value is then obtained by multiplying percentage land cover with the The 11 values obtained for the wetland are then added to obtain a wetland riparian Steps 1 through 5 above are repeated for the upland buffer zone to obtain an 5 98 Addition of the UPLSCORE and RIPSCORE then yields a wetland score called 99 WETSCORE which is then divided by 10 to obtain an overall Wetland Land Cover Index 100 (WLCI) value between 0 and 1 for a particular wetland. 101 102 WLCI UPLSCORE RIPSCORE 10 Equation 1 103 104 The LCIs calculated are then grouped into categories to represent the degree of impact. 105 These are: 0–0.29 (no impact), 0.30–0.45 (low impact), 0.46–0.61 (moderate impact), 106 0.62–0.77 (high impact), and >0.77 (very high impact). 107 The WLCA methodology developed in this study however may need some 108 adjustment for specific wetland types such as floodplains and mangroves. This is because 109 floodplains receive their water supply from catchments linked to the stream network such 110 that local land use around the floodplain is relatively unimportant. In contrast, mangroves 111 receive their water supply from streams and from the sea. It is thus proposed that in a 112 future study, the land-cover assessment methodology is extended to the watershed scale 113 for floodplains and not limited to the 1-km buffer. In the case of wetlands situated along 114 the coastal plains and which are directly influenced by the sea, such as mangroves, 115 further studies including modelling of current flow and contaminant movement in the 116 lagoon is suggested to get a clearer picture of the overall impact brought about by land 117 and sea pollution. 6