Columbia Stem Cell Day 2013
June 6, 2013
Myrna Daniels Auditorium and the Riverview Terrace; Vivian and Seymour
Family Heart Center; 173 Fort Washington Avenue, New York, NY 10032
Photo: Míriam Navarro Sobrino
PROGRAM
Program inquiries: Hynek Wichterle hw350@columbia.edu
General inquiries: Dola Sengupta ds2865@columbia.edu
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
2
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Columbia Stem Cell Day
June 6, 2013
9:30 AM
Breakfast and Registration
10:00 AM
Opening remarks
Hynek Wichterle and Joel Stein
10:05 AM
Stem cell approaches for epidermolysis bullosa using revertant mosaic iPSCs
Angela Christiano
10:25 AM
Cell Fate Restriction during C. elegans development
Tulsi Patel (Hobert lab)
10:40 AM
3
Adult neural stem cells and their niche: a dynamic duo during homeostasis and regeneration
Fiona Doetsch
11:00 AM
Coffee break
11:20 AM
Drug screens using patient specific iPS cells
Masayuki Yazawa
11:40 AM
Nervous system contributes to gastric cancer stem cell niche
Yoku Hayakawa (Wang lab)
11:55 AM
Genetic Dissection of the Hematopoietic Stem Cell Niche
Lei Ding
12:15 PM
LUNCH/Poster session
2:00 PM
Vision of CSCI
Lee Goldman, Executive Vice President for Health & Biomedical Sciences
Dean of the Faculties of Health Sciences & of Medicine
2:05 PM
Overview of CSCI
Chris Henderson
2:20 PM
Stem cells and the origin of prostate cancer
Michael Shen
2:40 PM
Parp1 and Tet2 coordinate chromatin modifications at an early stage of iPSC reprogramming
Claudia A. Doege (Abeliovich lab)
2:55 PM
Modeling stem cell asymmetry in budding yeast
Rodney Rothstein
3:15 PM
Coffee break
3:45 PM
A breath of fresh air: iPSC to the rescue
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Amy Firth (Verma lab, Salk Institute)
4:00 PM
4
Modeling Psychiatric diseases using iPSCs
Keynote Speech by Fred Gage, Salk Institute
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Short talks
4
Cell Fate Restriction during C. elegans development
Bogdanowicz, DR; Levine, WN; Morrison, B; and Lu, HH
Columbia University, New York, NY
Tulsi Patel and Oliver Hobert
Nervous system contributes to gastric cancer stem
cell niche
Yoku Hayakawa, Christoph B. Westphalen, Bernhard Renz,
Samuel Asfaha, Daniel L. Worthley, Yagnesh Tailor, Duan Chen,
and Timothy C. Wang
Parp1
and
Tet2
modifications at an
reprogramming
coordinate
chromatin
early stage of iPSC
Claudia A. Doege, Keiichi Inoue, Skylar Travis, David B. Rhee,
Ryousuke Fujita, Paolo Guarnier1, Alan Shih, Ross L. Levine,
Emily I. Chen, Asa Abeliovich
A Breath of Fresh Air: iPSC to the rescue
Amy L Firth and Inder M Verma
The Salk Institute
Posters
Heart Center Riverview Terrace
Methods for Directing Stem Cell Differentiation
5
Generation of insulin-producing cells in gut
organoids derived from human inducible
pluripotent stem cells
Ryotaro Bouchi, Kylie Foo, Haiqing Hua, Dieter Egli, Rudolph
Leibel, Domenico Accili
6
An Induced Pluripotent Stem Cell Model of PraderWilli Syndrome
Lisa C. Burnett1,2, Carlos R. Sulsona3, Daniel J. Driscoll3, Dieter
Egli1,4, Rudolph L. Leibel1,2,5
1 Columbia University, 2 Institute of Human Nutrition, 3 University of
Florida, 4 New York Stem Cell Foundation, 5 Naomi Berrie Diabetes
Center
7
Understanding ALS Disease Mechanisms using
human TDP-43 iPS-derived motor neurons.
Carrasco, M.A., Roberts, T., Skop., H, Friedman, B.A., Phatnani, H.,
Guernieri, P., Muratet M, O'Keeffe S., Bilacan, B., Nishimura A.L.,
Shaw CE, Chandran S., Maniatis, T.
8
1
Multiple ovarian follicle stem cells reside in the
germarium and contribute stochastically to follicle
cell daughters
Amy Reilein, Ari Berg, Natania Field, Elisa Cimetta, Nina
Tandon,Gordana Vunjak-Novakovic, and Daniel Kalderon.
1) Biological
Sciences; 2) Biomedical Engineering, Columbia
University, New York, NY.
HILO-RMCE based generation of iPS cells for
studying C9orf72 mutation in ALS
Vijay Chandrasekar1, Angelica Struve1, Neil Shneider2 and Tom
Maniatis1
Department of Biochemistry and Molecular Biophysics1, Department
of Neurology2, Columbia University Medical Center, New York, NY
10032
9
2
Examining the role of novel long noncoding RNAs in
beta cell biology
Luis Arnes1, Ildem Akerman2, Jorge Ferrer2 and Lori Sussel1
1Dept. of Genetics and Development, Columbia University, New York,
USA; 2Genomic Programming of Beta-cells Laboratory, Institut
d'Investigacions August Pi I Sunyer (IDIBAPS), Barcelona, Spain.
3
Hierarchical Patterning of Biomolecules with Sub50 nm Resolution
Nevette A. Bailey, Helen Tran, Kacey Ronaldson, Nathaniel A.
Lynd, Gordana Vunjak-Novakovic, and Luis M. Campos.
5
Maintaining anterior foregut spheres, derived from
human pluripotent stem cells, in a novel 3D culture
model
Ya-Wen Chen, Sarah Huang, Hans-Willem Snoeck
10
Prospective identification and purification of
quiescent and activated adult neural stem cells
from their in vivo niche
Paolo Codega, Violeta Silva-Vargas, Erika Pastrana, Angel R
Maldonado-Soto, Alex Paul, Annina DeLeo and Fiona Doetsch
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
11
17
Expanding the Synthetic Capabilities of the Cell
Increased adult hippocampal neurogenesis is
sufficient for antidepressant-like effects in a model
of chronic stress.
Virginia Cornish Group
12
The personalized immune mouse: a humanized
mouse model to study hematopoietic cell intrinsic
immune pathogenesis of type 1 diabetes
Nichole Danzl1, Hannes Kalscheuer1,5, Clare French1, Robert
Winchester2, Robin Goland2,3, Ellen Greenberg2,3, David G.
Savage4, Thomas Spitzer6, Hiroyuki Tahara1, Yong-Guang
Yang1,5, Megan Sykes1,5
1Columbia Center for Translational Immunology; 2Department of
Medicine; 3Naomi Berrie Diabetes Center; 4Division of
Hematology/Oncology at the College of Physicians and Surgeons,
Columbia University, New York, NY, USA.
5Transplantation Biology Research Center, Department of Surgery;
6Division of Bone Marrow Transplantation, Department of Medicine
at the Massachusetts General Hospital, Harvard Medical School,
Boston, MA, USA.
13
Specification of Cranial placodes from human
pluripotent cells
Alexis S. Hill1, René Hen1,2, Amar Sahay3-5
1Department of Neuroscience, Department of Psychiatry, Columbia
University, New York, New York; 2Division of Integrative
Neuroscience, The New York State Psychiatric Institute, New York,
New York; 3Center for Regenerative Medicine, Department of
Psychiatry, Massachusetts General Hospital, Boston, Massachusetts;
4Harvard Stem Cell Institute, Boston, Massachusetts; 5Harvard
Medical School, Boston, Massachusetts.
18
A stem cell model of diabetes due to glucokinase
deficiency
Haiqing Hua1,2, Linshan Shang2, Hector Martinez2, Matthew
Freeby 1, Thomas Ludwig 1 , Liyong Deng 1, Ellen Greenberg1,
Charles LeDuc1, Wendy Chung1, Robin Goland1, Rudolph Leibel1,
Dieter Egli2
1Division of Molecular Genetics, Department of Pediatrics and Naomi
Berrie Diabetes Center, Columbia University, New York, New York,
USA
2The New York Stem Cell Foundation Laboratory, New York, New
York, USA,
Zehra Dincer and Lorenz Studer
19
14
An in-vitro system to study NMJ dysfunction in ALS
Elena K. Kandror, Monica Carrasco, Tom Maniatis
15
Electromechanical Conditioning of Human Stem
Cell Derived Cardiomyocytes
George Eng1,2, Benjamin W. Lee1,2, Mark Gagliardi5, Kristy
Brown4, Gordon Keller5 and Gordana Vunjak-Novakovic1, 3
1Department of Biomedical Engineering, 2College of Physicians and
Surgeons, 3Department of Medicine, 4Department of Pathology and
Cell Biology, Columbia University, New York NY 10032; 5McEwen
Centre for Regenerative Medicine, University Health Network,
Toronto, Ontario M5G 1L7, Canada
16
Exosomes Mediate Stem Cell Crosstalk Between
Epithelium and Mesenchyme
Nan Jiang, Mo Chen, Guodong Yang and Jeremy J. Mao*
Center for Craniofacial Regeneration, Columbia University Medical
Center, New York, NY;
6
Human
hair
follicle
neogenesis
microenvironmentally reprogrammed
papilla cells
using
dermal
Claire A Higgins1, Colin AB Jahoda2, Angela M Christiano1,3
1: Department of Dermatology, Columbia University
2: School of Biological and Biomedical Sciences, Durham University
3: Department of Genetics and Development, Columbia University
20
Forming mechanosensing units in muscle cells
Thomas Iskratsch1, Giovanni Meacci1, Karen Maass2, Virginie
Stevenin1, Matthew Stachowiak3, Shuaimin Liu4, Ben
O’Shaughnessy3, James Hone4, Elisabeth Ehler5 and Michael
Sheetz1
1Department of Biological Sciences, Columbia University, New York,
NY; 2Department of Medicine, NYU, New York. 3Department of
Chemical Engineering, Columbia University, New York, NY;
4Department of Mechanical Engineering, Columbia University, New
York, NY; 5Randall Division of Cell and Molecular Biophysics and
Cardiovascular Division, King’s College, London, UK
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
1Project
21
Uncoupling of SMN requirement for motor neuron
survival and function in a stem cell model of the
motor circuit
Anna M. Janas1,2, Christian M. Simon1,2, Francesco Lotti1,2,
George Z. Mentis1,2,3, Livio Pellizzoni1,2
1. Center for Motor Neuron Biology and Disease,
2. Depts. of Pathology and Cell Biology and 3. Neurology, Columbia
University, NY, 10032
A.L.S./Jenifer Estess Laboratory for Stem Cell Research,
Departments of Regenerative Medicine, Pathology, Neurology and
Neuroscience, Center for Motor Neuron Biology and Disease (MNC),
Columbia Stem Cell Initiative (CSCI), Columbia University Medical
Center, New York, USA; 2 iPierian, Inc., South San Francisco,
California, USA; 3The Howard Hughes Medical Institute, Harvard
Stem Cell Institute, Department of Stem Cell and Regenerative
Biology, Department of Molecular and Cellular Biology, Harvard
University, Cambridge, Massachusetts, USA
27
22
PDGFRb in the adult SVZ stem cell lineage
Coupling of Hedgehog and Hippo pathways
promotes stem cell maintenance by stimulating
proliferation
Angel R. Maldonado-Soto, Paolo Codega, Alex Paul, Annina M.
DeLeo, and Fiona Doetsch
Jianhua Huang and Daniel Kalderon
Department of Biological Sciences, Columbia University, New York,
NY10027, USA
23
Columbia Genome Center
Screening Core Facility
High
Throughput
Andrea Califano, Christopher E. Henderson, Charles Karan, Hai
Li, Sergey Pampou, Ronald Realubit
28
Generation of induced pluripotent stem cells from
revertant mosaic keratinocytes and fibroblasts
Noriko Umegaki 1, Anna M.G. Pasmooij 3, Zongyou Guo 1,
Munenari Itoh 1, Antoni Gostynski 3, Marcel F. Jonkman 3 and
Angela M. Christiano 1, 2
Departments of Dermatology 1 and Genetics and Development 2,
Columbia University, New York; Department of Dermatology,
University of Groningen, Groningen 3
29
24
The Role of Prdm16 and Mitochondrial Dynamics
in Hematopoietic Stem Cell Function
Larry Luchsinger, Kenjiro Kamezaki, David Corrigan, Eric A.
Schon & Hans-Willem Snoeck
25
Identification of the Migratory Precursor to Human
Dendritic Cells
Jaeyop Lee, Ines Matos, Sarah Puhr, Carolyn Lee, Kang Liu
Department of Microbiology and Immunology, Columbia University
Medical College, New York
26
An SMN-dependent, cell-autonomous survival
deficit in human Type 1 SMA ES-derived motor
neurons
Mathieu Desclaux1, Yoon A. Kim1, Alejandro Garcia Diaz1, Shila
Mekhoubad3, John Dimos2,3, Kevin Eggan3, Hynek Wichterle1 and
Christopher Henderson1.
Extracellular Matrix Derived from Kidney
Regulates the Growth and Metabolism of Kidney
Stem Cells with Regional Specificity
J. D. O'Neill, D. O. Freytes, A. Anandappa, J. A. Oliver, and G.
Vunjak-Novakovic
30
Personalized Medicine: Choloroquine Toxicity in
human RPE is Dependent on ARMS2 and HTRA1
Genotypes
Eric Chi-Hsien Peng1,3 Yao Li1, Chyuan-Sheng Lin2, Stephen H.
Tsang1.
1Bernard and Shirlee Brown Glaucoma Laboratory, Department of
Pathology and Cell Biology, College of Physicians and Surgeons,
Columbia University, New York, New York, United States of America
2. Herbert Irving Comprehensive Cancer Center, Columbia University,
New York, New York, United States of America
3. Shin Kong Wu Ho-Su Memorial Hospital and Catholic Fu Jen
university, Taipei, Taiwan.
31
Experimental approaches to elucidate cell of origin
of bladder cancer using GEM models
Tomasz B. Owczarek, Cory Abate-Shen
Departments of Urology, Pathology & Cell Biology, College of
Physicians and Surgeons, Herbert Irving Comprehensive Cancer
Center, Columbia University Medical Center, New York, NY
7
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
32
37
Using Embryonic Stem Cells To Study Motor
Neuron-Glia Interactions In ALS
A novel fluorescent reporter system for in vivo
visualization of hematopoietic stem cell dynamics
in the bone marrow niche
Hemali P. Phatnania, Paolo Guarnierib,*, Brad Friedmanc,*,
Monica A. Carrascoa, Michael Muratetd, Sean O’Keeffea,
Chiamaka Nwakezea, Florencia Pauli-Behnd, Kimberly M.
Newberryd, Sarah K. Meadowsd, Juan Carlos Tapiae, Richard M.
Myersd, and Tom Maniatisa
aColumbia University Medical Center, Department of Biochemistry &
Molecular Biophysics, New York, NY 10032; bColumbia University
Medical Center, Columbia Initiative in Systems Biology, Herbert Irving
Comprehensive Cancer Center, New York, NY 10032; cHarvard
University, Department of Molecular and Cellular Biology, Cambridge,
MA 02138; present address: Genentech, Inc., Department of
Bioinformatics and Computational Biology, South San Francisco, CA
94080; dHudsonAlpha Institute for Biotechnology, Huntsville,
Alabama 35806; eColumbia University Medical Center, Department of
Neuroscience, New York, NY 10032; *These authors contributed
equally to this work
33
Loss of the ubiquitin-specific protease usp-48
allows for direct conversion of a somatic tissue into
neurons in Caenorhabditis elegans
Dylan P. Rahe1, Tulsi Patel2, and Oliver Hobert1,2,3
1. Department of Biological Sciences 2. Department of Genetics and
Development 3. Howard Hughes Medical Institute and Department of
Biochemistry and Molecular Biophysics, Columbia University, New
York NY
34
The Columbia NYSTEM CPS Facility Design and
Synthesis of Chemical Probes
Rachid Skouta1, Brent R. Stockwell1, 2, 3, 4
1 Chemical Probe Synthesis facility, 2 Howard Hughes Medical
Institute, 3 Department of Biological Sciences, 4 Department of
Chemistry, Columbia University
35
The Organic Chemistry Collaborative Center (OCCC)
Black Building 1029, CUMC Campus
Shi-Xian Deng 1,2,3, Donald Landry1,2,3, Alison Rinderspacher1,2,3
1Department of Medicine; 2Columbia University Medical Center;
3Columbia Stem Cell Initiative
36
Generation of airway and lung epithelial cells from
human pluripotent stem cells
Sarah X.L. Huang1, Ya-Wen Chen1, Melanie Mumau1, HansWillem Snoeck1
1Columbia Center for Translational Immunology, Columbia University
Medical Center, New York, NY, USA
8
Katie Sawai and Boris Reizis
38
Targeted gene correction of type VII collagen in
recessive dystrophic epidermolysis bullosa patientspecific induced pluripotent stem cells
Satoru Shinkuma1, Noriko Umegaki1, Munenari Itoh1, 2, Rodolfo
Murillas3, Marcela Del Río3, 4, Fernando Larcher3, Angela M
Christiano1, 5
1. Departments of Dermatology, Columbia University, New York
2. Department of Dermatology, Jikei University School of Medicine,
Tokyo, Japan
3. Epithelial Biomedicine Division, CIEMAT-CIBERER, Madrid, Spain
4. Department of Bioengineering, Madrid, Spain
5. Department of Genetics and Development, Columbia University,
New York, U.S.A.
39
Cerebrospinal fluid regulation of adult neural stem
cells
Violeta Silva Vargas and Fiona Doetsch.
40
The effects of an ALS-causing TDP-43 mutation on
RNA binding and Motor Neuron Survival
Helaina Skop*, Monica Carrasco*, Aarti Sharma*, Lei Lu*, Yuan
Yuan#, Robert Darnell#, Neil Shneder*, Tom Maniatis*
*Columbia University, #Rockefeller University
41
Investigating
amyotrophic
lateral
sclerosis
pathophysiology by functional characterization of
motor neurons generated from mouse embryonic
stem cells
John Smerdon1, Sebastian Thams2, Christopher E. Henderson2,3,4,
Hynek Wichterle2,3,4, and Amy B. MacDermott1,3, Damian J.
Williams1
1Department of Physiology and Cellular Biophysics, Columbia
University, New York, NY. 2Project A.L.S./Jenifer Estess Laboratory for
Stem Cell Research, New York, NY. 3Department of Neuroscience,
Columbia University, New York, NY. 4Departments of Pathology,
Neurology and Neuroscience, Center for Motor Neuron Biology and
Disease (MNC), and Columbia Stem Cell Initiative (CSCI), Columbia
University, New York, NY.
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
42
Nkx Genes Regulate Second Heart Field
Development at the Poles of theHeart through
Opposing Mechanisms
Sophie Colombo1, Vanessa George1 and Kimara L. Targoff1
1Division of Cardiology, Department of Pediatrics, College of
Physicians & Surgeons, Columbia University, New York, NY, 10032.
1 Division of Molecular Genetics and Naomi Berrie Diabetes Center,
Columbia University College of Physicians and Surgeons, New York,
NY 10032. 2 New York Stem Cell Foundation. 3 Institute of Human
Nutrition, Columbia University, New York, NY 10032. 4 Department
of Physiology & Cellular Biophysics, Columbia University, New York,
NY 10032. 5 Department of Ophthalmology, Columbia University,
New York, NY10032.
48
43
Nkx2.2: a major regulator of the intestinal
epithelium?
Stefanie Gross1, Dina Balderes1, Samuel Asfaha2 and Lori Sussel1
1Department {Vassoler, 2013 #6329}of Genetics and Development,
Columbia University Medical Center, New York, USA
2Division of Digestive and Liver Diseases, Irving Cancer Research
Center, Columbia University Medical Center, New York, USA.
Molecular identity and specification of crossed
versus uncrossed retinal ganglion cells
Qing Wang, Takaaki Kuwajima, and Carol A. Mason
Doctoral Program in Neurobiology and Behavior; Departments of
Pathology and Cell Biology, Neuroscience, and Ophthalmology,
College of Physicians and Surgeons
49
The transcription factor ZFX controls self-renewal
of acute leukemia
44
Biomimetic electrical stimulation platform for
neural differentiation of retinal progenitor cells
N Tandon1, E Cimetta1, E deBernardinis1, A Taubman1, N
Kupferstein2, U Madaan3, J Mighty3, S Redenti3 and G Vunjak--‐
Novakovic1
1Dept. Of Biomedical Engineering, Columbia University, Dept. of
Electrical Engineering, The Cooper Union for the Advancement of
Science and Art2, Dept. of Molecular Cell and Developmental Biology,
Lehman College City of New York
45
Combining genet ic and pharmacologic means to
achieve specific, inducible, and reversible
enhancement of adult hippocampal neurogenesis
Tzong-Shiue (Tony) Yu, Jing Du, Autumn Kim, and Steven G.
Kernie
46
Asymmetric B lymphocyte division in the early
immune response
William C. Adams and Steven L. Reiner
Department of Microbiology and Immunology, Columbia University
47
Stem cell-based approaches to the neurobiology of
obesity in bardet-biedl syndrome
Liheng Wang1,2,3, Damian Williams4, Stephen H. Tsang5, Robin S.
Goland1, Amy B. MacDermott4, Dieter Egli1,2, Rudolph L. Leibel1,3
9
Stuart P. Weisberg1, Matthew R. Smith-Raska1, Jose M. Esquilin2,
Michael Churchill3, Teresita L. Arenzana1, Ji Zhang4, Haiyan
Pan1, Apostolos Klinakis5, Jack E. Dixon4, Siddhartha Mukherjee3
and Boris Reizis1*
1Department of Microbiology and Immunology, Columbia University
Medical Center, New York, NY, 10032, USA; 2Division of Pediatric
Hematology, Columbia University Medical Center, New York, NY,
10032, USA; 3Department of Medicine, Division of Oncology, Columbia
University Medical Center, New York, NY, 10032, USA; 4Department of
Pharmacology, University of California, San Diego, La Jolla, CA; 5
Biomedical Research Foundation, Academy of Athens, Athens 11527,
Greece
50
Cytokine-dependent Regulation of SOD1 Astrocyte
Toxicity in Amyotrophic Lateral Sclerosis
Andrew H. Williams1, Jacqueline de Vegvar1, Jonathan Sisti1,
Thomas M. Jessell1,2,3, and Tom Maniatis1
1Department of Biochemistry and Molecular Biophysics, 2Department
of Neuroscience, 3Howard Hughes Medical Institute, Columbia
University Medical Center, New York, NY
51
Gremlin 1 defines a mesenchymal stem cell in the
gastrointestinal
tract,
bone
and
tumor
microenvironment
Worthley DL, Churchill M, Si Y, Asfaha S, Manieri N, Westphalen
CB, Tailor Y, Hayakawa Y, Carpenter J, Nair A, Jin G, Quante M,
Glaire M, Renz B, Pradere J-P, Troeger J, Schwabe R, Levin D,
Nizami S, Grikscheit T, Stappenbeck T, Lee F, Mukherjee S, Wang
TC.
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
52
Potential role of HDC as a regulator of HSC myeloid
lineage differentiation
Xiaowei Chen,1 Lei Ding,2 Samuel Asfaha,1 Ashlesha Muley,1
Yagnesh Tailor1 Karan Nagar,1 Daniel L Worthley,1 Bernhard W
Renz1 Guangchun Jin, 1
Yoku Hayakawa,1 Christoph B.
Westphalen,1 Timothy C Wang1
Division of Digestive and Liver Disease, Department of Medicine and
Herbert Irving Comprehensive Cancer Center, Columbia University,
New York, NY, USA. Department of Microbiology and Immunology,
Department of Rehabilitation and Regenerative Medicine, Columbia
University Medical Center, New York, NY, USA.
53
Transcriptional regulation of retinoic acid-induced
neural lineage commitment in mouse embryonic
stem cells
Yuan-Ping Huang1, Esteban O. Mazzoni2 and Hynek Wichterle1
1Departments of Pathology and Cell Biology, and Neuroscience,
Center for Motor Neuron Biology and Disease, and Columbia Stem
Cell Initiative, Columbia University, New York, NY 10032, USA, U.S.A.
2Department of Biology, New York University, New York, NY, U.S.A.
54
Identification of master regulators of pluripotency
in mouse epiblast stem cells
Hui Zhao1,2,3, Mariano Alvarez1,4,5, Celine Lefebvre1,4,5, Andrea
Califano1,4,5 and Michael M. Shen1,2,3.
1Herbert Irving Comprehensive Cancer Center, 2Department of
Medicine, 3Department of Genetics and Development, 4Joint Centers
for Systems Biology, 5Department of Biomedical Informatics,
Columbia University Medical Center, New York, NY 10032.
55
Generation of 3D Full-Thickness Skin Equivalents
Exclusively From Human induced Pluripotent Stem
Cell (iPSC)-Derived Keratinocytes and Fibroblasts
Zongyou Guo 1, Munenari Itoh 1, Noriko Umegaki 1 and Angela M.
Christiano 1, 2
Departments of Dermatology 1 and Genetics and Development 2,
Columbia University, New York
10