Revised on July 3rd, 2015 TIGP-MCB Experimental Approaches in Molecular and Cell Biology for Fall 2015 Course code: 3024180 Credits: 2 (lectures: 2 hr per week, in English) Organizers: Dr. Nan-Shih Liao Time: Monday, 3:30 pm-5:20 pm Place: Auditorium, 1st floor, Institute of Molecular Biology, Academia Sinica Student Evaluation: Homework or in-class quiz assigned by each lecturer Date Topic and engineering Lecturer 1 09/07 Cloning vectors production for protein 2 09/14 Protein purification and characterization Hanna Yuan 3 09/21 Introduction to X-ray crystallography David Hsiao 4 10/05 Methods to detect protein-protein interactions Chih-Yen King 5 10/12 Gene transcription and chromatin modeling Hung-Ta Chen 6 10/19 Methods to study RNA & control of gene expression at RNA level Huey-Nan Wu 7 10/26 High Throughput Next Generation Sequencing (I) Mei-Yeh Lu 8 11/02 High Throughput Next Generation Sequencing (II) Mei-Yeh Lu 9 11/09 Bioinformatic Analysis of NGS Data 10 11/16 Model organism I: Virus Wen Chang 11 11/23 Model organism II: Yeast Tien-Hsien Chang 12 11/30 Model organism III: Drosophila Ya-Hui Chou 13 12/07 Model organism IV: Arabidopsis Yi-Fang Tsay 14 12/14 Model organism V: Mouse 15 12/21 Protein localization 16 12/28 A Brief History Of Light Microscopy And How It Transformed Biomedical Research 12/28 Final-term Exam(Submit Final Score Due) Yu-Chan Chao Wei-Che Hsu Ching-Yen Tsai Hsou-min Li Suewei Lin