Name _______________________________
Since all cells in our body contain DNA, there are lots of places for mutations to occur; however, some mutations cannot be passed on to offspring and do not matter for evolution. Somatic mutations occur in non-reproductive cells and won't be passed onto offspring. For example, the golden color on half of this Red
Delicious apple was caused by a somatic mutation. Its seeds will not carry the mutation.
Enrichment
The only mutations that matter to large-scale evolution are those that can be passed on to offspring. These occur in reproductive cells like eggs and sperm and are called germ line mutations .
Effects of germ line mutations
A single germ line mutation can have a range of effects:
1.
No change occurs in phenotype.
Some mutations don't have any noticeable effect on the phenotype of an organism. This can happen in many situations: perhaps the mutation occurs in a stretch of DNA with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affecting the amino acid sequence of the protein .
2.
Small change occurs in phenotype.
A single mutation caused this cat's ears to curl backwards slightly.
3.
Big change occurs in phenotype.
Some really important phenotypic changes, like DDT resistance in insects are sometimes caused by single mutations. A single mutation can also have strong negative effects for the organism. Mutations that cause the death of an organism are called lethals — and it doesn't get more negative than that.
Little mutations with big effects: Mutations to control genes
Mutations are often the victims of bad press — unfairly stereotyped as unimportant or as a cause of genetic disease. While many mutations do indeed have small or negative effects, another sort of mutation gets less airtime. Mutations to control genes can have major (and sometimes positive) effects.

Some regions of DNA control other genes, determining when and where other genes are turned "on". Mutations in these parts of the genome can substantially change the way the organism is built. The difference between a mutation to a control gene and a mutation to a less powerful gene is a bit like the difference between whispering an instruction to the trumpet player in an orchestra versus whispering it to the orchestra's conductor. The impact of changing the conductor's behavior is much bigger and more coordinated than changing the behavior of an individual orchestra member. Similarly, a mutation in a gene "conductor" can cause a cascade of effects in the behavior of genes under its control.
Many organisms have powerful control genes that determine how the body is laid out. For example, Hox genes are found in many animals (including flies and humans) and designate where the head goes and which regions of the body grow appendages. Such master control genes help direct the building of body "units," such as segments, limbs, and eyes. So evolving a major change in basic body layout may not be so unlikely; it may simply require a change in a Hox gene and the favor of natural selection .
Mutations to control genes can transform one body part into another. Scientists have studied flies carrying Hoxmutations that sprout legs on their foreheads instead of antennae!

Point mutations : a single nucleotide is changed, added, or removed.
1.
Substitution : one nucleotide is replaced with another.
These can be “silent” if the amino acid sequence is not changed.
2.
Deletion : one nucleotide is removed from the DNA strand.
3.
Insertion : one nucleotide is added to the DNA strand.
Original DNA strand: THE FAT CAT ATE THE RAT
(pretend that these are nucleotides, you can read them and it makes sense)
Determine whether the following examples are due to a substitution, deletion, or insertion:
1.
THE FAC ATA TET HER AT ______________________
2.
THE EFA TCA TAT ETH ERA T ______________________
3.
THE FTT CAT ATE THE RAT ______________________
4.
THE FAT CAT ATE THR AT ______________________
5.
THE FAT CAT ATE ETH ERA T ______________________
6.
THT FAT CAT ATE THE RAT ______________________
Chromosomal mutations: changes in the number or structure of chromosomes.

Deletions : involve the loss of all or part of a chromosome.

Duplications : produce extra copies of parts of a chromosome.

Inversions : reverse the direction of parts of chromosomes.

Translocations : when one part of a chromosome breaks off and attaches to another.
Substitution
Insertion
Deletion

Mutations Worksheet Name Per.
In each of the following DNA sequences, you will use the mRNA and amino acid sequences to identify the mutation that occurred and the effects of each on, if any. Look and analyze carefully! classify each as substitution, silent substitution, insertion, or deletion.
Original DNA Sequence: T A C, A C C, T T G, G C G, A C G, A C T mRNA Sequence:
Amino Acid Sequence:
Mutated DNA Sequence #1: T A C, A T C, T T G, G C G, A C G, A C T
What’s the mRNA sequence? (Circle the change)
What will be the amino acid sequence?
Will there likely be effects?
What kind of mutation is this?
Mutated DNA Sequence #2: T A C, G A C, C T T, G G C, G A C, G A C, T
What’s the mRNA sequence? (Circle the change)
What will be the amino acid sequence?
Will there likely be effects?
What kind of mutation is this?
Mutated DNA Sequence #3: T A C, A C C, T T A, G C G, A C G, A C T
What’s the mRNA sequence? (Circle the change)
What will be the amino acid sequence?
Will there likely be effects?
What kind of mutation is this?
Mutated DNA Sequence #4: T A C, A C C, T T G, G C G, A C T, A C T
What’s the mRNA sequence? (Circle the change)
What will be the amino acid sequence?
Will there likely be effects?
What kind of mutation is this?
1.
Which type of mutations are the most dangerous?
2.
WHY Can DNA MUTATIONS be SO HARMFUL TO YOUR BODY?