Hannah Winters 5/26/10 Abstract Ever since blood typing has been investigated, many new medical advancements have been achieved, saving the lives of many. Not only does blood typing save lives, it can determine who a father is of a child, and it could lead to more medical advances. In the experiment, we needed to determine who the father of a baby is by comparing its blood to the mothers, and two possible fathers. In the procedure we took the four different samples of synthetic blood and placed them into a depression on the blood type slide. We added anti A, B, and Rh serum to the samples to determine what type of blood the person had. The blood showed the Puck more likely the father compared to Finn. The experiment was very simple and it would be difficult to make an error. Not only did the experiment explain how blood typing works, but it gave you a real life example, even if a television show isn’t real life. Hannah Winters 5/26/10 Genetics’ Formal Lab Report Ever since Pope Innocent VIII received a blood transfusion but then later died, blood typing and blood transfusions has been investigated. Back then some trials and testing were banned because of a high death rate because it wasn’t known that your body would reject different blood types. But fortunately for us, Karl Landsteiner, the most important person in the area of blood transfusions, discovered the three different blood types found in humans, A, B, and O. From that birthed the same blood types we have today, A+, A-, B+, B-, AB+, AB-, O+, and O-. Since then many lives have been saved by blood transfusions. But blood typing isn’t only used for blood transfusions; it can also determine parents of a child by blood types and whether or not the child is Rh+ or Rh-. If you have a + blood type, then you have Rh antigens, you don’t have them if you are Rh-. All these factors are important if you are getting a blood transfusion because you don’t want to get the wrong type of blood in your system. These factors sum up the importance and the basis of blood typing and transfusion. Without all this, we wouldn’t have the amazing medical technology like we do today. In the blood typing lab we needed to figure out who the father of a baby was using the known blood types of the mother, possible fathers, and child. If Puck is the father of the child, then his blood type and Quinn’s blood type will make possible genotypes which the child has because the child needs a trait, dominate or recessive, from each parent. Hannah Winters 5/26/10 Experiment Materials Blood type slide 10 toothpicks Anti-A serum Anti-B serum Anti-Rh serum 4 blood samples Procedure After the materials were prepared, we added a drop of synthetic blood into each depression in the first row on the slide. We repeated this step with each type of blood. Then we added the anti serums and mixed them with a toothpick. We then recorded if the blood clumped together or not. We analyzed the results, if the blood clumped together, that meant that the blood was positive for whatever antiserum was used. Hannah Winters 5/26/10 Results Observations Sample of Blood Before Experiment During Experiment After Experiment 1 Liquidy Two clumped A antiserum clumped, so did the Rh. 2 Liquidy Two clumped B antiserum clumped, along with the Rh. 3 Liquidy Two clumped A antiserum clumped and so did the B. 4 Liquidy One clumped Only Rh antiserum clumped. Hannah Winters 5/26/10 Conclusion The experiment showed that Quinn, or blood sample 1, was positive for Rh, and she had blood type A. All of those blood samples had clumped, while the others stayed a liquid. Puck’s blood, blood sample 2, was positive for Rh and he is blood type B. Blood sample 3, Finn, was negative for Rh, but both A and B antiserums clumped, which means he is AB-. The baby’s blood, blood sample 4, was only positive for Rh, which means the baby is O+. These results follow my hypothesis, that Puck was the father of the baby, not Finn. This experiment left little room for error, and it helped with understanding how blood typing works. It was a very simple procedure , all you had to do was take the blood samples and add the antiserums to find out if the blood type was positive for the antiserum or not, to do that you have to see if it clumps or not. I wouldn’t change the procedure because it was very simple, but if I wanted to add anything to the procedure I would add something more to investigate and make the lab a little more complex. I would like to see something more, though I am unsure what to add because the simple lab covered a lot. Hannah Winters 5/26/10 References "History of Blood Transfusion Medicine - BloodBook, Blood Information for Life." Blood Information Blood Test Results, Blood Transfusion, Blood Disorders and Health. N.p., n.d. Web. 28 May 2010. <http://www.bloodbook.com/trans-history.html>. O'Neil, Dennis . "Rh Blood Types." anthro.palomar.edu. N.p., n.d. Web. 28 May 2010. <anthro.palomar.edu/blood/Rh_system