1. A study was undertaken of the evolution of two species of wasps, one the parasite of the other. The parasite queen, Vespula squamosa, kills the host queen, Vespula maculifrons, and takes over her role in the colony. Data was compiled for 13 colonies of V. squamosa and 37 colonies of V. maculifrons to analyse the genetic structure of the two species. Each point on the graphs represents the genetic distance and geographic distance between a pair of colonies. The genetic distance indicates the number of differences in specific DNA markers between a pair of colonies. The results are shown below. (a) i. Determine the greatest genetic distance between any pair of colonies of V. squamosa. [1] (a) ii. Identify the greatest geographic distance between any pair of colonies of V. maculifrons. [1] (b) Describe the relationship between the geographic distance and genetic distance in the two species. [1} (c) Compare the two species in terms of genetic distance between the pairs of colonies. [2] (d) Data collected on eight specific alleles in the two species indicated that each species was in Hardy–Weinberg equilibrium. Evaluate all the data regarding possible evolutionary changes within the two species. [2] 2. Discuss the incompleteness of the fossil record and the resulting uncertainties about human evolution [6]. 3. (a) i. Distinguish between transient polymorphism and balanced polymorphism. [2] (a) ii. State an example of transient polymorphism [1] (b) Describe an example of a barrier between gene pools. [2] (c) Outline how variations in DNA can indicate phylogeny. [2] 4. (a) Define the term clade. [1] (b) Suggest two reasons for using cladograms for the classification of organisms. [2] (c) Distinguish between analogous and homologous structures, giving an example of each. [2] (d) The table below lists five animals along with four morphological characteristics. A plus sign (+) indicates that the animal has this characteristic while a minus sign (-) indicates that the characteristic is absent. Based on the features, a student constructed a cladogram. State the names of the organisms missing in the following cladogram. [2] (e) Describe the major anatomical features that define humans as primates. [2] 3. Discuss the endosymbiotic theory for the origin of eukaryotes. [6] Markscheme: 1. 2. few hominid fossils have been found/not often found/are very rare; most hominids/organisms not preserved/decompose; only teeth and bones remain / soft tissues do not fossilize; require certain conditions for preservation/dry/anaerobic/quickly covered/frozen; earlier cultures did not bury the dead / remains were scavenged/dispersed; acids break down/dissolve teeth/bones; many missing links/fossils of intermediate stages; difficult to get conclusive evidence / difficult to falsify theories; theories change radically with one/few discoveries; 3. (a) i. transient when one allele gradually replaces another / directional selection; balanced when allele frequencies do not change / no/stabilizing selection; (ii) e.g. gradual increase in darker peppered moths due to environmental change/ industrial melanism Accept any other valid example. (b) name of type of barrier; description; e.g. behavioural isolation; different courtship behaviour so no mating between two populations; Accept only the first type of barrier in the answer. Accept geographical separation, hybrid infertility, difference in chromosome number or breeding time. (c) variation due to random mutations / count numbers of difference in base sequence; can be used as evolutionary clock / estimate time since divergence/separation; amount of variation indicates how closely species are related/linked; differences can indicate ancestry/sequence in which groups diverged; 4. (a) a group of related organisms sharing a common ancestor / a group of organisms containing an ancestor and all of its descendants / OWTTE (b) methods used to prepare cladograms use a different approach from traditional classification/taxonomy; show ancestral relationships; reflect how recently two groups shared a common ancestry; cladograms are (objective/accurate because they are usually) based on molecular differences; they should be considered as a good complement to traditional classification; (c) homologous structures evolved from a common ancestor while analogous structures did not; example of homologous and example of analogous; (both needed) e.g. an example of homologous is pentadactyl limb in mammals and birds / mouth parts in house fly and mosquito/other valid example and an example of analogous is eye in vertebrates and squid/octopus / wings of insect and bat / jointed legs of vertebrates and insects/other valid example (d) (e) opposable thumbs (enable grabbing); flat fingernails (instead of claws/for scratching); forward-facing eyes for stereoscopic vision; rotatable shoulder joints (for tree-climbing adaptation); skull modified for upright posture; large brain to body/skull ratio enables higher levels of thinking; 3. eukaryotes evolved from prokaryotes; mitochondria/chloroplasts evolved from (independent) prokaryotic cells; taken in by larger (heterotrophic) cell by endocytosis; theory supported by characteristics of chloroplasts/mitochondria; [2 max] for mitochondria/chloroplast characteristics: mitochondria/chloroplasts have naked DNA; mitochondria/chloroplasts divide/carry out fission; mitochondria/chloroplasts have 70S ribosomes / synthezise own proteins; mitochondria/chloroplasts have double membranes; cristae similar to mesosomes / thylakoid have similar structures in prokaryotes; but theory cannot be falsified as it predicts something occurring in the past; theory does not explain the origins of cilia/flagella/linear chromosomes/meiosis; weaker evidence that cilia/flagella evolved from attached bacteria/spirochetes;