1
Supplementary figure captions
2
3
Fig. S1 Species accumulation curves for the four extensively sampled logs at site F, based on
4
number of samples taken per log. Thirty-two samples were taken for all four logs, but not all of
5
them amplified successfully.
6
Fig. S2 CCA plot showing distances among the eight samples taken at position B on the 12 logs
7
at site F. Sample positions are indicated: 1, 2 mean left or right side of log; the same unfilled and
8
filled symbol represents the outer and inner sample from the same upper or lower position on the
9
log.
10
Fig. S3 Fungal community composition in logs, f – site F, g – site G. (a) Levels of abundance of
11
the different ecological groups: WD – wood decayers, Sa – saprophytes, SP – both saprophytic
12
and parasitic strategy, PP – plant parasites, Mp – mycoparasites, My – mycorrhizal fungi, Li –
13
lichenized fungi, OG – other ecological roles, Unk – no data about ecology available. (b) OTU
14
numbers in the ecological groups. (c) Phylogenetic composition - abundances, (d) OTU numbers
15
in the ecological groups; A – Ascomycetes, B –Basidiomycetes, unk – unidentified to lower
16
taxonomical level than Ascomycetes or Basidiomycetes, id – identified.
17
Fig. S4 Fungal community composition at different positions in logs (f – site F, g – site G). (a)
18
Levels of abundance of the different ecological groups: WD – wood decayers, Sa – saprophytes,
19
SP – both saprophytic and parasitic strategy, PP – plant parasites, Mp – mycoparasites, My –
20
mycorrhizal fungi, Li – lichenized fungi, OG – other ecological roles, Unk – no data about
21
ecology available. (b) OTU numbers in the ecological groups. (c) Phylogenetic composition-
22
abundances, (d) OTU numbers in the ecological groups; A – Ascomycetes, B –Basidiomycetes,
23
unk – unidentified to lower taxonomical level than Ascomycetes or Basidiomycetes, id –
1
24
identified. The ecological differences among logs were larger than differences among positions
25
within logs.
26
Fig. S5 CCA plots showing OTUs with the highest fit and weight values to the decay stage of the
27
outer samples at sites F (a) and G (b).
28
Fig. S6 Fungal community composition in decay classes – abundances of ecological (a) and
29
taxonomical (b) groups (a), for abbreviations see legend of Fig. S2 and Fig. S3. Class f8 and g6
30
were not shown because of the low number of samples in these decay classes.
31
32
2
33
Log 1
Log 4
Log 6
Log 9
N. of detected species
400
300
200
100
0
0
10
15
20
25
30
35
N. of samples per log
34
35
5
Fig. S1
36
3
1.0
Up Out 1
Down Out 1
Up In 1
Down Out 2
Down In 1
Up In 2
-1.0
Up Out 2
-1.0
37
38
39
40
Down In 2
1.0
Fig. S2
4
41
Percentage of sequences
0
20
40
60
80
100
f1 f2 f3 f4 f5 f6 f7 f8 f9 f10 f11 f12 g1 g3 g5 g7 g8 g10g11g13g15g16g21g25g26
42
43
Site and log
wd
sa
sp
pp
mp
my
li
og
unk
a)
Percentage of OTUs
0
20
40
60
80
b)
5
G26
G25
G21
G16
G15
G13
G11
G8
G10
G7
G5
G3
F12
F11
F9
F10
F8
F7
F6
F5
F4
F3
F1
G1
Site and log
wd
sa
sp
pp
mp
my
li
og
unk
44
45
F2
100
Percentage of sequences
0
20
40
60
80
100
f1
f3
f4
f5
f6
f7
f8
f9 f10 f11 f12 g1 g3 g5 g7 g8 g10 g11 g13 g15 g16 g21 g25 g26
Site and log
unk A
id A
unk B
id B
other groups
unclassified
46
47
f2
c)
Percentage of OTUs
0
20
40
60
80
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G3
G5
G7
G8
G10
G11
G13
G15
G16
G21
G25
G26
100
unk A
id A
unk B
id B
other groups
unclassified
48
49
d)
50
Fig. S3
Site and log
51
6
55
54
b)
7
G18
G30
G42
G do
52
G up
G in
G ou
FD
FC
FB
FA
F do
F up
53
F in
F ou
Percentage of OTUs
G18
G30
G42
G do
G up
G in
G ou
FD
FC
FB
FA
F do
F up
F in
F ou
Percentage of sequences
0
20
40
60
wd
sa
sp
pp
mp
my
li
og
unk
80
100
Site and position
a)
0
20
40
60
wd
sa
sp
pp
mp
my
li
og
unk
80
100
Site and position
unk A
id A
unk B
id B
unclassified
20
40
60
80
G18
G30
G42
Gdo
Site and position
56
57
G up
G in
G ou
FD
FC
FB
FA
F do
F up
F in
100
F ou
Percentage of sequences
0
c)
Percentage of OTUs
0
20
40
60
80
59
d)
60
Fig. S4
G 18
G30
G 42
G down
G up
Site and position
unk A
id A
unk B
id B
other groups
unclassified
58
G in
G out
FD
FC
FB
FA
F down
F up
F in
F out
100
8
1.0
Sar gla
Thele
42
Het par
Bot bot
Res
bic
Chaet 16
Cat bra Hyp min
Hyp pal
Cal vis Lep ela
-1.0
Agari124 347 Chlo vir
Chaet 78 Cla cha
A79
Fom pin
F decay Pen spi
Phial
sp
A189 A251
Hyp hyp
Pau pea
Helot 80 B201 Rhi atr B135 Hyp hym
Dacry 57 Hyp alu
Coni 17
Chaet 88
Leu sor
Ath sub
Cortic34 A243
Phall 86 B31 Corti 60
-1.0
1.0
61
62
a)
-1.0
1.0
63
Amp bys Ath neu
Helot191
Trech119
Str hor 68
Sebac499
Lec lut 162 B40 Tub bor
Tyl fib Phial sp Can pal
A100
Asc cyl
B201 B76 Trem 10
Phial 77
Cla con B35 G decay
Helot248
Chaet379
Helot212
Verruc97 Agaro141 Helot174 Pen spi
Phi dim
Verruc 6 Botry sp Helot203
Cla con
Verruc 4 Chaet 16
Verru277
Agyri103 Sord 39
Aca liv Col abi
-1.0
1.0
64
65
b)
66
Fig. S5
67
68
69
9
Percentage of OTUs
0
20
40
60
80
100
f1
f2
f4
f5
f6
f7
f9 g1 g2 g3 g4 g5 g7 g8 g9
Site and decay class
WD
Sa
SP
PP
Mp
My
Li
OG
Unk
70
71
f3
a)
Percentage of OTUs
0
20
40
60
80
100
f1
f2
f3
f4
f5
f6
f7
f9 g1 g2 g3 g4 g5 g7 g8 g9
Site and decay class
unk A
id A
unk B
id B
other groups
unclassified
72
73
b)
74
Fig. S6
10