Mosaic Origin of the Heme Biosynthesis Pathway in Photosynthetic Eukaryotes The evolution of metabolic pathways is a subject of much current interest, with a number of different hypotheses to explain the origin of new enzymes (Nara, Hshimoto, and Aoki 2000; Illingworth et al. 2003; Schmidt et al. 2003). In this work, we address the next level of evolution: gene acquisition/replacement related to the endosymbiotic origins of organelles. Because all known prokaryotes, eubacteria and archaea, require heme for numerous cellular functions, it is safe to assume that both the original amitochondriate eukaryote (assumed to have arisen from the archaeal lineage) and the a-proteobacterium that became the mitochondrion were able to synthesize heme. However, several enzymes in the pathway do not appear to be of cyanobacterial origin. In the case of porphobilinogen deaminase, the plastid enzymes are clearly related to those of a-proteobacteria Importance of it: Heme biosynthesis represents one of the most essential metabolic pathways in living organisms, providing the precursors for cytochrome prosthetic groups, photosynthetic pigments, and vitamin B(12). Phylogenetic analyses showed the mosaic character of this pathway in photosynthetic eukaryotes. Nyllaine Ann M. Rodriguez Grade 9- Maaasahan Take Home UT – Biology. Metabolic Pathway. 2. Nonphotosynthetic Heterokonts Phytophthora species are plant pathogens classified asoomycetes, the sister group to the photosynthetic heterokonts(e.g., diatoms). It is still unclear whether the oomycetes once had a chloroplast and lost it or whether the photosynthetic heterokonts acquired plastids after the two branches of the family separated. 3. How would this Heme Biosynthesis will really affect the body if these synthesis act as enzymes? 4. Heme contained in the food is taken up from the intestines, but this is only relevant because of the iron it contains. The organic porphyrin ring is mostly synthesized from scratch. The synthetic pathway is split across two compartments; the initial and final steps occur in the mitochondria, while the intervening ones occur in the cytosol. Heme exercises feedback inhibition on the first step in the pathway. Mosaic Origin of the Heme Biosynthesis Pathway in Photosynthetic Eukaryotes Mol. Biol. Evol. 22(12):2343–2353. 2005 doi:10.1093/molbev/msi230 Advance Access publication August 10, 2005 The Author 2005. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org Accepted July 18, 2005 References: http://mbe.oxfordjournals.org/content/22/12/2343.full.pdf http://www.ncbi.nlm.nih.gov/pubmed/16093570 http://www.gopixpic.com/485/hemebiosynthesis/http:%7C%7Cwww*sivabio*50webs*com%7Cplas026*jpg/ http://watcut.uwaterloo.ca/webnotes/Metabolism/ironHemeBiosynthesis.html