Association between the MUC5B Promoter Polymorphism rs35705950 and Idiopathic Pulmonary Fibrosis: a Meta-analysis
and Trial Sequential Analysis in Caucasian and Asian Populations
Qing-Qing Zhu, MD, Xin-Lin Zhang, MD,Si-Min Zhang, MM, Shao-Wen Tang, PhD, Hai-Yan Min, PhD, Long Yi, PhD, Biao Xu, MD, PhD, Yong Song, MD,
PhD
Search strategy in PubMed:(“Idiopathic pulmonary fibrosis” or “IPF” or “Usual interstitial pneumonia” or “UIP” or “Cryptogenic FibrosingAlveolitis” or
“Hamman Rich Disease” or “Fibrocystic Pulmonary Dysplasia”) and (rs35705950 or (("MUC5B" or "mucin 5B" or "MG1" or "MUC5" or "MUC9" or
"MUC-5B") and ("polymorphism" or "mutation" or "variation")))
Association between the MUC5B Promoter Polymorphism rs35705950 and Idiopathic Pulmonary Fibrosis: a Meta-analysis and Trial Sequential Analysis in Caucasian
and Asian Populations
Qing-Qing Zhu, MD, Xin-Lin Zhang, MD,Si-Min Zhang, MM, Shao-Wen Tang, PhD, Hai-Yan Min, PhD, Long Yi, PhD, Biao Xu, MD, PhD, Yong Song, MD,
PhD
Table S1.Quality assessment of studies using the Newcastle-Ottawa Scale (NOS), and the definitions of IPF patients and controls.
First author
Year
Ethnicity
Selection
Comparability
Exposure
Total
IPF patients
Controls
Seibold, et al
2011
Caucasian
4
1
3
8
A COPD case-control cross-sectional study,
excluded self-reported or had a physician-diagnosis
of idiopathic pulmonary fibrosis or another
interstitial lung disease.
Borie, et al
2013
Caucasian
3
1
3
7
Noth, et al
2013
Caucasian
4
1
3
8
Stock, et al
2013
Caucasian
4
2
3
9
The 2001 American Thoracic Society/European
Respiratory Society consensus (A high resolution
computerized tomography (HRCT) scan was
required to show definite or probable IIP according
to predefined criteria, a surgical lung biopsy was
obtained in 46% of affected subjects.)
The 2001 American Thoracic Society/European
Respiratory Society consensus (either surgical
biopsy or a characteristic CT scan pattern)
The 2000 and 2011 guidelines from the American
Thoracic Society and European Respiratory
Society (A high-resolution CT scan that showed
definite or probable usual interstitial pneumonitis
was necessary for inclusion. A surgical lung biopsy
sample to confirm usual interstitial pneumonitis
could be obtained if the diagnosis was in doubt, a
surgical lung biopsy was obtained in 37.3% of
subjects)
The 2001 criteria of the American Thoracic
Society/European Respiratory Society
Healthy unrelated individuals who were ethnically
matched.
Healthy individuals free of lung diseases based on
ICD9 coding and medical record review.
Healthy blood donors based on a self-administered
questionnaire and by the routine laboratory
investigations performed on blood donors.
Horimasu, et al
2014
Asian
4
1
3
8
The 2001 criteria of the American Thoracic
Society/European Respiratory Society
Horimasu, et al
2014
Caucasian
4
1
3
8
The criteria of the American Thoracic
Society/European Respiratory Society in 2002
Wang, et al
2014
Asian
4
2
3
9
Peljto, et al.
2015
Caucasian
3
2
3
8
Peljto, et al.
2015
Asian
3
2
3
8
The 2011 American Thoracic Society/European
Respiratory Society International Consensus,
exclusion of the known causes of interstitial lung
disease (clinical features and HRCT evaluation,
and ahistopathologic diagnosis was made for the
cases with an atypical HRCT image)
The 2011 American Thoracic Society/European
Respiratory Society International Consensus
Guidelines
The 2011 American Thoracic Society/European
Respiratory Society International Consensus
Guidelines
Healthy subjects underwent pulmonary function tests
and chest X-ray studies, excluded those with
apparent lung disease such as interstitial lung
diseases (ILD) or chronic obstructive pulmonary
disease.
Healthy subjects underwent pulmonary function tests
and chest X-ray studies, excluded those with
apparent lung disease such as interstitial lung
diseases (ILD) or chronic obstructive pulmonary
disease.
Healthy individuals who were undergoing a routine
physical examination at the same hospital
NA
Control subjects with at least 5 years of smoking
history (former and current) and normal pulmonary
function tests were recruited from a smoking
cessation program at the same institute, and
nonsmoking control subjects were unrelated healthy
volunteers.
The Newcastle-Ottawa Scale (NOS) assesses the study quality in 3 categories: selection, comparability, and exposure. A maximum of 4 stars could be given to
selection items, 2 stars to comparability section and 3 stars to Exposure category. NA=not available.
Figure S1. Trial sequential analysis of rs35705950 polymorphism and IPF risk using the allelic model (T allele versus G allele) in
Caucasian population.
Figure S2. Trial sequential analysis of rs35705950 polymorphism and IPF risk using the allelic model (T allele versus G allele) in Asian
population.
Figure S3. Funnel plot assessing evidence of publication bias from the eligible studies using the allelic model (T allele versus
G allele).
Figure S4. Funnel plot assessing evidence of publication bias from the eligible studies using the genotypic model (GT genotype
versus GG genotype).
Figure S5. Trial sequential analysis of rs35705950 polymorphism and IPF risk using the genotypic model (GT genotype versus GG
genotype) in Caucasian population.
Figure S6. Trial sequential analysis of rs35705950 polymorphism and IPF risk using the genotypic model (GT genotype versus GG
genotype) in Asian population.