Questions G11 Biology 2.1 Cell Theory 1. What are the functions of life, as demonstrated by all living organisms? Nutrition, growth , response to stimuli, reproduction, homeostasis, movement, order 2. Define the following terms, with examples: Unicellular: made of one cell Multicellular: made of many cells Acellular: not made of cells 3. Why are viruses often considered non-living? Not composed of cells Only carry out life processes whilst inside another cell 4. Outline the three fundamental statements of cell theory i. All living things are made of cells ii. All cells come from pre-existing cells iii. Cells are the smallest structural unit that carries out the life processes 5. What is the significance of the discoveries made by Antonie van Leeuwenhoek and Robert Hooke? Leeuwenhoek – discovered protists (animalicules) Hooke – named the cell 6. Some types of cell seem to break the laws of cell theory. a. Give two examples of cells which are multinucleated Fungal hyphae/muscle cells b. Why are viruses often considered ‘acellular’ or even non-living? Acellular – because not made of cells Non- living – because only carry out the life processes whilst inside another cell 7. Complete this table of SI units of length: Metric Equivalent Whole scientific notation Unit Abbreviation kilometer km 1 000 m 103 m Meter m 1m - centimeter cm 0.1 10-2 m Millimeter mm 0.001 m 10-3 m micrometer μm 0.000 001 m nanometer nm 0.000 000 001 10 Mag = imagesize/actual size Mag = 25mm/2µm Mag = 25 000 µm/2µm = X 12 500 b. By which method (shown here) do bacteria reproduce? Binary fission m 10-9 m 8. The diagram below shows the characteristic rod-shaped structure of E. coli bacteria. a. What is the magnification of the image? –6 9. What is the actual size of the structures delineated in yellow? Actual size = image size/magnification a. b. c. d. 55mm/1000 = 0.055mm OR 55µm 75mm/5000 = 0.015mm OR 15µm 45mm/200 = 0.225mm OR 225µm 25mm/100 = 0.25mm OR 250µm 10. Calculate the magnification of these scale bars: Mag = Image size (scale bar)/actual size a. b. c. d. e. f. g. 17mm/2µm = 17000µm/2µm = X8500 17mm/67µm = 17000µm/67µm = X253 33mm/100nm = 33 000 000nm/100nm = X330,000 33mm/100µm = X330 20mm/50µm = X 400 20mm/50mm =X0.4 23mm/500m = X 0.000046 11. What is the magnification of these images? a. Scale bar 10µm measures 40mm on the image. Mag = Image/actual Mag = 40mm/10µm = 40 000 µm/10µm = X4000 b. Scale bar 5µm measures 25mm on the image. Mag = 25 000µm/5µm = X5000 12. A micrograph has a scale bar of 2µm, which measures 40mm on the image. Measuring the maximum length of the cell in the image, the ruler reads 180mm. How long is the cell? 40mm = 2 µm 1mm = 2/40 = 0.05 µm 180 mm = 9µm 13. A student views an image of a cell magnified 350 times. The image is 250mm long. What is the actual length of the sample in the image? Actual size = image size/magnification Actual size = 250mm/350 = 0.71mm OR 710µm 14. Compare the sizes of these structures. Use SI units. Plant cell Animal cell nucleus bacteria Mitochondria virus ribosome Membrane thickness molecules 40µm 20µm 5µm 0.5 – 1.0 µm 0.5 – 1.0 µm 10nm 20nm 10nm 1nm 15. As the volume of a cell increases, what happens to…? (increase/ decrease) a. Production of waste products. Increases b. Usage of nutrients and oxygen. Increases c. The surface area: volume ratio. Decreases 16. What are the advantages of maximizing the surface area: volume ratio in a cell? More membrane per unit area of the cell/ cell can supply its needs more efficiently 17. What strategies do cells use to maintain an efficient SA:Vol? Folding of membranes/ keep cells small/ compartments within the cell 18. What are some of the ways in which larger organisms maximize SA:Vol? Elephants – large ears 19. How can a large SA:Vol be harmful or costly to smaller animal species? Small animals will lose heat to their environment very quickly/they will need to eat all the time in order to replace this lost energy 20. Unicellular organisms carry out all the functions of life, multi-cellular organisms differentiate and show emergent properties. a. What is meant by the term emergent properties? Sum is more than its parts Watch has complex parts but when it is put together it shows properties that were nto evident previously b. What are the advantages of cells differentiating to carry out specific functions? Division of labour/ cells can specialize/ more efficient at one job 21. All cells in a living organism carry the same genetic information. a. What is a stem cell? An undifferentiated cell capable of differentiating and producing more stem cells b. What are the following types of stem cells? Pluripotent: can differentiate into any type of body cell Multipotent: can differentiate into several types of body cells Nullipotent: cannot divide c. What type of cell can a liver stem cell become? A liver cell 22. Outline the process of cell differentiation that leads from an uncommitted stem cell to a specialized cell, including the role of gene expression. A flow chart might help. Uncommitted stem cell environmental signal (chemical) key genes are activiated cell differentiation specialized cell 23. Give three examples of specialized cells in multicellular organisms. Describe how their structure relates to their function. i. Nerves: elongated/myelinated/many dendrites ii. Retina: light sensitive iii. Muscle: large number of mitochondria to provide energy for contraction 24. Complete the table below to show how stem cells can be used in medicine. Therapeutic cloning Parkinson’s disease Alzheimer’s Used to treat Diabetes Skin Brief method: Involves the culturing of stem cells outside of the body. These can then be used to replace damaged tissue. Stem cell transplants Bone marrow (leukemia) Ethical Use of embryonic stem cells/sacrifice of considerations one human life to save another. Adult stem cells are used/not so controversial Stem cells can be harvested, bone marrow destroyed. Stem cells can then be used to replace the bone marrow 25. What are the objections of some groups to the use of embryonic stem cells? Taking of human lives Possible use in reproductive cloning Cosmetic use