S1. Specification of patients’ cohort with bacterial infections. Table includes information
about localization of wound, patients’ age and sex, associated diseases and infection severity.
Patients were divided into groups according to treatment duration (< 4 weeks; 4 - 7 weeks; ≥ 8
weeks and one dead patient).
< 4 weeks
Group by
Patient
treatment
duration
Localization of wound
Age
Sex
1
Abdomen
70
male
2
DM Ulcus cruris
69
male
3
Sinus pilonid
35
male
7
Scrotal abscess
43
male
8
Decubitus of right foot
35
female
10
Wound infection after OP
93
female
20
The defect of the sacral region
after excision of pivonid. Sinus
21
male
24
Carbuncle of neck
70
female
25
Infections of lacerations of
second finger
57
26
Trophic defect of sole left
lower limb with osteomyelitis
of headers
67
28
Varicose ulcer
49
29
Perianal abscess - perineum
19
male
31
Carbuncle of neck
36
male
1
Associated
diseases
Infection
severity
Treatment
duration
deep
3 weeks
surface
3 weeks
deep
2 weeks
surface
3 weeks
surface
2 weeks
surface
3 weeks
Sine
deep
2 weeks
Sine
surface
3 weeks
deep
3 weeks
deep
3 weeks
surface
3 weeks
Salmonelosis
surface
2 weeks
Morbid obesity,
hypertension,
surface
3 weeks
Ischemic heart
disease
Peripheral
arterial disease,
DM,
hypertension
Sine
Hypertension,
obesity
Pes
equinovarus
bilat.
DM,
hypertension
Hypertension,
gout
DM 1 - insulin,
Alzheimer’s
disease,
dementia,
female chronic renal
insufficiency,
coronary artery
disease,
hypothyroidism
Casus socialis,
chronic
alcoholism and
male
smoking,
contamination
of leg ulcers by
fly larvae
male
DM 2
32
The abdominal wall
83
34
Right knee - post-traumatic
abscess
29
35
38
Defect of right instep
Abscess of the left forearm
after insect bites
39
70
23
83
Infection after gastritic
resection
40
36
Carbuncle of neck
41
42
49
Varicose ulcer
The infected excoriations in the
elbow
44
76
59
Osteomyelitis of left calcaneus
with fistula
45
An infected wound of the right
72
2
Peptic
duodenal ulcer
penetrating into
the head of the
pancreas female resection of the
stomach, then
reoperation for
dehiscence of
the duodenal
stump
male
Sine
Surgery of the
cervical spine,
quadruparesis,
immunosuppres
sion for
female
rheumatoid
arthritis,
hypertension,
peripheral
arterial disease
Abscess of the
left forearm
male
after insect
bites
Resection of
the stomach,
infection
female
around the
abdominal
drain
Hypertension,
male DM 1 - insulin,
morbid obesity
Casus socialis,
contamination
male
of ulcer by fly
larvae,
enterocolitis
Coronary heart
female
disease, obesity
Hypertension,
chronic
male warfarinisation
for atrial
fibrillation
female
Hypertension
deep
3 weeks
surface
2 weeks
surface
3 weeks
deep
3 weeks
deep
3 weeks
surface
2 weeks
surface
3 weeks
surface
2 weeks
deep
3 weeks
deep
3 weeks
ankle
47
4 - 7 weeks
50
Varicose ulcer
Abscess and phlegmon of the
chest wall
35
male
50
male
13
Infection after external fixator
of right thigh-MRSA
68
female
15
Varicose ulcer
82
female
21
Decubitus of heel, DM - insulin
78
male
27
The wound after the upper
middle laparotomy
59
male
30
Perianal abscess
43
female
33
Dehiscent wound after
laparotomy - abdominal wall
72
female
36
Decubitus of sacrum
88
female
3
Casus socialis
Hypertension,
obesity, post
ATB clostridial
enterocolitis
Chronic
osteomyelitis
of the right
femur,
hypertension
Myeloproliferat
ive syndrome,
immunosuppres
sion corticosteroids
DM 2,
hypertension
Rheumatoid
arthritis immunosuppres
sion, cardiac
arrhythmias,
prostatic
hyperplasia
Depression,
DM 2,
operation: HYE
adnexectomy
left, cystectomy
heterotopic
neovesica,
polyvalent drug
allergy
Hypertension,
hyperlipidemia,
operation of
Hodgkin's
lymphoma of
the spleen,
chemotherapy
+ radiotherapy
for carcinoma
of the vulva
APPE, chronic
nicotine abuse
Hypertension,
obesity, atrial
fibrillation,
coronary heart
surface
3 weeks
deep
3 weeks
deep
1 month
surface
1 month
deep
6 weeks
deep
5 weeks
deep
4 weeks
deep
6 weeks
deep
4 weeks
≥ 8 weeks
37
Decubitus of right knee
88
female
43
Phlegmon and abscess of the
chest wall
50
male
46
Decubitus of right knee
82
female
48
Decubitus of right heel
70
male
49
Infection after osteosynthesis
65
female
4
Infection of the external fixator
DM
85
female
5
Perianal abscess
55
female
6
Post-traumatic defect
58
male
9
Periproctal abscess DM
43
female
11
Sacral decubitus
36
male
12
Infection after extraction of the
external fixator of l. tibia
62
male
14
Diabetic gangrene of thumb
(amputation stump)
72
female
17
Pressure ulcers of cross-field
83
male
4
disease,
osteoporosis,
immobility
Hypertension,
obesity, atrial
fibrillation,
coronary heart
disease,
osteoporosis,
immobility
Hypertension,
obesity
Obesity,
hypertension,
ischemic heart
disease
Hypertension,
obesity, DM2,
polycythaemia
vera
Hypertension,
rheumatoid
arthritis immunosuppres
sion with
corticosteroids,
bladder
carcinoma
DM 2, chronic
obstructive
pulmonary
disease,
ischemic heart
disease
Ulcerative
colitis
surface
4 weeks
deep
4 weeks
deep
6 weeks
surface
6 weeks
deep
4 weeks
deep
months
surface
2 months
Psoriasis
deep
2 months
DM 2
deep
months
deep
months
surface
months
deep
3 months
deep
months
DM, obesity,
triplegia after
craniotrauma
Chronic posttraumatic
osteomyelitis
of tibia
DM 1,
hypertension,
obesity
Ischemic heart
disease,
paraplegia
Exitus
18
Recurrence of perianal fistula
55
female
19
Varicose ulcer
73
male
22
Varicose ulcer
73
male
23
Sacral decubitus
73
male
16
Diabetic gangrene of V. finger
(amputation stump)
72
male
5
Ulcerative
colitis
Parkinson’s
disease
Parkinson’s
disease
Parkinson’s
disease
DM1, acute
hepatic and
renal failure
surface
2 months
surface
months
surface
months
surface
months
deep
12 days
S2. 108 identified microorganisms (MO) in patients’ wounds by MALDI-TOF MS using MALDI
Biotyper™ software comparing protein profiles (2-30 kDa) with BDAL database. In case of incorrect
identification (score < 2), the bacterial strains were confirmed by Sanger sequencing of 16S bacterial
gene. The virulence of Staphylococcus aureus infection was investigated by presence of mecA and
fnbA genes.
Growth MO identified by MALDI-TOF
Sample
Score
medium
MS
1
b
Hafnia alvei DSM 30097 DSM
1.681
Hafnia alvei NC4221
c
Hafnia alvei DSM 30101 DSM
1.588
Hafnia alvei NC4221
Corynebacterium striatum
QC0411
Pseudomonas aeruginosa
NCGM 1900
Corynebacterium striatum
QC0411
a
2
c
a
3
4
d
a
b
a
5
6
7
d
b
b
b
a
8
9
d
e
b
10
MO identified by DNA
sequencing
c
Corynebacterium striatum DSM
20668T DSM
Klebsiella oxytoca DSM 5175T
HAM
Corynebacterium striatum
23086514 MLD
Enterococcus faecalis DSM
20409 DSM
Candida lambica(ana)
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Pseudomonas hydrocarboxydans
B333 UFL
Enterococcus faecium DSM
22502
Pseudomonas hydrocarboxydans
B333 UFL
Staphylococcus hominis ssp.
hominis
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Streptococcus agalactiae
1.769
1.644
1.525
2.065
Staphylococcus haemolyticus
KMGL1309-SO8
Corynebacterium striatum
1.742
ATCC 6940
Staphylococcus aureus subsp. not1.565
aureus SA268
present present
1.502
2.021
Staphylococcus aureus subsp. notnot1.806
aureus SA268
present present
1.678
Neisseria mucosa
Staphylococcus aureus subsp. not1.406
aureus SA268
present present
Staphylococcus aureus subsp. not1.845
aureus SA268
present present
Streptococcus agalactiae
1.710
NGBS061
Lactobacillus parabuchneri DSM
5707T DSM
1.422
Shigella flexneri VITRNJ
Pseudomonas aeruginosa 19955
Pseudomonas aeruginosa BP
1 CHB
2.012
C2
Pseudomonas aeruginosa 19955
Staphylococcus
aureus
mecA
fnbA
gene
gene
2.020 Pseudomonas aeruginosa BP
6
1 CHB
b
a
c
11
d
b
12
a
b
13
a
c
b
a
14
d
c
15
b
c
b
a
16
d
b
c
17
a
b
a
18
19
20
e
b
b
Proteus vulgaris DSM 30119
DSM
Staphylococcus aureus ATCC
33591 THL
Pseudomonas nitroreducens
DSM 14399T HAM
Enterococcus faecalis DSM
20409 DSM
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Staphylococcus aureus ATCC
25923 THL
Staphylococcus aureus ATCC
33591 THL
Staphylococcus aureus ATCC
33591 THL
Hafnia alvei 20740 1CH
Staphylococcus aureus spp.
aureus DSM 4910 DSM
Staphylococcus aureus spp.
aureus DSM 4910 DSM
Actinocorallia libanotica B246
UFL
Escherichia coli MB11464 1CH
Pseudomonas mendocina DSM
50017T HAM
Raoutella ornithinolytica MB
18887 CHB
Proteus vulgaris DSM 3265 DSM
Staphylococcus epidermis ATCC
14990T THL
Enterococcus faecalis DSM
20409 DSM
Pseudomonas aeruginosa 19955
1CH
Pseudomonas aeruginosa ATCC
27853 CHB
Candida albicans ATCC 10231
THL
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Staphylococcus aureus ATCC
29213 THL
Enterococcus faecalis MB11464
1CH
Pseudomonas aeruginosa 19955
1 CHB
Staphylococcus lugdunensis DSM
6670 DSM
C2
1.904
Proteus vulgaris LSRC158
Staphylococcus aureus subsp. not1.762
aureus SA268
present present
Escherichia coli O25b:H41.392
ST131 EC958
2.094
notpresent present
Staphylococcus aureus subsp. notpresent
1.781
aureus SA268
present
Staphylococcus aureus subsp.
present
1.522
aureus SA268
present
Staphylococcus aureus subsp.
present
1.804
aureus SA268
present
2.004
2.350
notpresent present
notpresent present
2.362
2.147
1.443
Enterococcus faecalis KLDS
6.0316
2.081
2.076
1.957
Klebsiella oxytoca M1
1.877
Enterococcus faecalis E12
2.051
2.253
2.075
2.029
Staphylococcus aureus subsp.
present present
1.458
aureus SA268
present present
2.161
notpresent
present
2.048
2.039
2.074
1.701
7
Staphylococcus lugdunensis
Inje L3
21
22
a
c
c
23
d
b
24
a
b
a
25
26
c
1.421
1.781
Escherichia coli MB11646 CHB
2.233
d
Enterococcus faecalis 20247
CHB
2.321
e
Escherichia coli MB11464 CHB
2.389
e
c
b
2.290
2.034
2.372
2.235
2.098
2.115
2.387
2.345
b
Escherichia coli ATCC 25922
THL
2.250
c
Escherichia coli MB11464 CHB
2.209
a
Enterococcus faecalis 20247
CHB
Staphylococcus aureus ssp.
aureus DSM 4610 DSM
Staphylococcus aureus ATCC
33591 THL
notpresent
present
Staphylococcus aureus subsp. notpresent
1.956
aureus AMRF1
present
Escherichia coli MB11464 CHB
d
Staphylococcus lugdunensis
Inje L3
Staphylococcus aureus
ATCC 43300
2.295
c
b
31
2.194
c
b
30
2.075
a
Pseudomonas aeruginosa 8147
CHB
Staphylococcus epidermidis
ATCC 14990T THL
Enterococcus faecium 20218
CHB
Pseudomonas aeruginosa 8147
CHB
Acinetobacter genomospecies
Serovar 18 DSM 9341 DSM
Acinetobacter genomospecies
Serovar 18 DSM 9341 DSM
Klebsiella pneumoniae ssp
pneumoniae 9295 CHB
notpresent
present
2.105
Staphylococcus aureus ssp.
aureus DSM 4610 DSM
d
29
Pseudomonas aeruginosa
1.998
GR4RAU-A1
Staphylococcus haemolyticus
1.601
KMGL1309-SO8
Streptococcus agalactiae
1.646
NGBS061
Escherichia coli MB11464 CHB
a
28
2.069
b
b
27
Staphylococcus epidermis 10547
CHB
Pseudomonas aeruginosa DSM
50071T HAM
Staphylococcus haemolyticus Mb
18803 2CHB
Staphylococcus haemolyticus
10024 CHB
Staphylococcus aureus ssp.
aureus DSM 4910 DSM
Staphylococcus epidermis 6b s
ESL
Staphylococcus epidermis ATCC
14990T THL
Staphylococcus lugdunensis DSM
4805 DSM
Aeromonas caviae CECT 838T
DSM
2.291
2.155
2.276
8
notpresent
present
notpresent
present
b
c
32
d
b
33
34
c
b
b
35
c
b
c
36
d
b
37
38
c
e
a
c
d
39
e
b
a
40
41
42
e
e
e
b
a
43
e
Acinetobacter baumannii DSM
30007T HAM
Acinetobacter baumannii DSM
30007T HAM
Enterococcus faecalis 20247
CHB
Klebsiella pneumoniae ssp
pneumoniae 9295 CHB
Klebsiella pneumoniae ssp
pneumoniae 9295 CHB
Acinetobacter lwoffii DSM 2403T
DSM
Pseudomonas aeruginosa 8147
CHB
Pseudomonas aeruginosa DSM
1117 DSM
Pseudomonas aeruginosa DSM
1117 DSM
Escherichia coli MB11464 CHB
Enterococcus faecalis DSM 2570
DSM
Acinetobacter baumannii LMG
994 HAM
Acinetobacter baumannii GEIH
2000 HCB
Staphylococcus aureus ssp aureus
DSM 4910 DSM
Staphylococcus epidermidis
10547 CHB
Stenotrophomonas maltopilia
(PX) 230862229 MLD
Enterococcus faecium 20218
CHB
Enterococcus faecalis ATCC
29212 CHB
Staphylococcus aureus ATCC
33862 THL
Staphylococcus aureus ssp aureus
DSM 4910 DSM
Staphylococcus aureus ssp aureus
DSM 4910 DSM
Staphylococcus aureus ssp aureus
DSM 346 DSM
Staphylococcus aureus ssp aureus
DSM 20232 DSM
Staphylococcus aureus ssp aureus
DSM 4910 DSM
Staphylococcus aureus ssp aureus
DSM 4910 DSM
2.341
2.270
2.281
2.332
2.497
2.023
2.291
2.265
2.416
2.120
2.266
2.337
2.288
notpresent
present
2.060
2.025
2.244
2.012
1.753
2.105
2.072
2.187
2.008
2.122
2.082
2.153
Staphylococcus aureus ssp aureus 2.044
9
Enterococcus faecalis E12
notpresent
notpresent
notpresent
notpresent
notpresent
notpresent
notpresent
notpresent
not-
not-
present
present
present
present
present
present
DSM 346 DSM
44
45
e
e
b
46
47
c
a
b
48
49
a
b
b
a
50
c
Staphylococcus aureus ssp aureus
DSM 20232 DSM
Staphylococcus aureus ssp aureus
DSM 346 DSM
Enterobacter cloacae 13159 1
CHB
Enterobacter cloacae MB11506 1
CHB
Staphylococcus cohnii ssp cohnii
DSM 20261 DSM
Staphylococcus aureus ATCC
29213 THL
Staphylococcus aureus ssp aureus
DSM 346 DSM
Staphylococcus aureus ATCC
29213 THL
Staphylococcus aureus ssp aureus
DSM 346 DSM
Staphylococcus aureus ssp aureus
DSM 346 DSM
Staphylococcus epidermidis DSM
1798 DSM
present present
notpresent
present
Staphylococcus aureus subsp. notnot1.882
aureus SA268
present present
2.037
2.045
2.036
2.012
2.124
2.066
2.254
1.764
1.764
2.012
10
notpresent
notpresent
notpresent
Staphylococcus aureus subsp. notaureus SA268
present
Staphylococcus aureus subsp. notaureus SA268
present
present
present
present
present
present