Supplementary figure legends
Supplementary Fig. S1. Screening of the CaCcs promoter from Capsicum genomic library. A.
Hybridization signal with 32P-CaCcs full-length cDNA on 1st plaque screening. B.
Hybridization signal with 32P-CaCcs full-length cDNA on 2nd plaque screening. C. Three
restriction enzyme digest patterns of a putative CaCcs genomic clone. D. Southern
hybridization of a putative CaCcs genomic clone with a 32P-0.2 kb fragment of N-terminal
region of CaCcs cDNA. Lane 1, EcoRI; Lane 2, HincII; Lane 3, HpaI. The resulting EcoRIdigested 2.5-kb fragment containing 2.3 kb of 5′ proximal promoter and 0.2 kb of the Nterminal coding region of the CaCcs gene was subcloned into pSK as pSK-CaCcs-G-2.5kb.
Supplementary Fig. S2. DNA sequences of the 5’-proximal promoter region for CaCcs and
schematic illustration of deletion vector constructs for driving Gus gene expression. A. DNA
sequence of a 2,555-bp EcoRI fragment containing 2,309 bp of the 5' promoter (upper case)
and 246 bp of N-terminal coding region (lower case) of CaCcs. Restriction enzyme sites used
for 5'-deletion series of CaCcs promoter are indicated in blue above the nucleotide
sequences. Nucleotides shown in red indicate the transcriptional (+1) and translational
(methionine) start sites. B. Scheme for subcloning the CaCcs promoter (CaCcsP6) and its 5′deletion fragments (CaCcs-P5, P4, P3, P2, P1) into pBluescript (pKS) and fusion with a βglucuronidase gene (Gus) and Nos terminator (Tnos). C. Size confirmation of the full-length
CaCcs promoter and its deletion fragments by restriction enzyme digestion. Common 2.2-kb
fragments indicated in all six lanes are Gus:Tnos fragments.
Supplementary data
Table S1. Scan results of predicted cis-acting motifs in the region of –367 to +30 of the
Capsicum Ccs promoter.
No. Motif name
Signal sequence
Number of signal Species origin
Promoter origin
Binding protein
Spatial specificity
Stress-regulator
chloroplast
blue, white or UV-A light
1
-10PE
TATTCT
1
Barley
psbD
2
ARR1
NGATT
4
Arabidopsis
NSHB
3
CAAT box1
CAAT
7
Pea
legA
4
CACT motif
YACT
7
Flaveria trinervia
ppcA1
5
CArG motif
CWWWWWWWWG
6
Arabidopsis
6
CCAAT box1
CCAAT
2
Soybean
7
GATA box
GATA
2
CaMV, petunia, Arabidopsis , rice CaMV 35S, petunia Cab22
8
GT-1 consensus
GRWAAW
2
Pea, oat, rice, tobacco,
Arabidopsis , spinach, bean
many rbcS et al.
9
GT-1
GAAAAA
1
Soybean
SCaM-4
10
GT-1 motif
KWGTGRWAAWRW
1
Pea
rbcS-3A
11
GTGA motif
GTGA
5
Tobacco
g10
12
INR element
YTCANTYY
3
Tobacco
psaDb
13
MYB1 element
GTTAGTT
1
Arabidopsis , tomato
Pti4(ERF)
14
MYB2
TAACTG
1
Arabidopsis
15
MYB2 consensus
YAACKG
1
Arabidopsis
16
MYB core
CNGTTR
1
Arabidopsis , petunia
17
NODCON2/
OSE2ROOTNODULE
CTCTT
2
Soybean, Vicia faba
Ibc3, N23, VfLb29
18
POLLEN1
AGAAA
6
Tomato
lat52, LeMAN5
19
RAV1A
CAACA
1
Arabidopsis
20
ROOT motif
ATATT
2
Agrobacterium
rolD
21
SEF4 motif
RTTTTTR
3
Soybean
7S globulin
SEF4
embryo (seed)
22
TAAAG motif
TAAAG
1
Potato
KST1
StDof1
guard cell
23
T box
ACTTTG
1
Arabidopsis
GAPB
Supplementary Fig. S1
ARR1
cytokinin-regulated
seed
mesophyll
AGL15
heat shock protein
flower
flowering
ASF-2
light
light
pathogen and salt
GT-1
light
pollen
light
defense
ATMYB2
water stress (dehydration)
rd22
water stress (dehydration)
ATMYB1, ATMYB2 petal epidermiss
water stress (dehydration)
root nodule
anther, pollen
RAV1
rosette leaves, roots
root
light