Purification and Identification of Novel Antioxidative Peptide Released from Black-Bone Silky
Fowl (Gallus gallus domesticus Brisson) Muscle
Jianhua Liu b, Yousheng Huang c, Yinggang Tian a, Shaoping Nie a, Jianhua Xie a, Yong Wang a,
Mingyong Xie a, *
a
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R.
China
b
Department of Food Science and Engineering, College of Biological and Environmental Engineering,
Zhejiang University of Technology, Hangzhou 310014, P. R. China
c
Analytical and Testing Center of Jiangxi Province, Nanchang 330029, P. R. China
*Corresponding author. Tel.: +86 791 83969009; fax: +86 791 83969009.
E-mail address: myxie@ncu.edu.cn (M. Y. Xie).
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Supporting information
Fig. 1. The UV absorbance curves from 210 nm to 395 nm of both subfraction 4-3 (a) and 8-3 (b) were
nearly identical.
0 .8 0
0 .7 0
234.4
0 .6 0
au
au
0 .5 0
0 .4 0
0 .3 0
0 .2 0
0 .1 0
311.2
349.3
375.4 393.4
0 .0 0
2 2 0 .0 0
2 4 0 .0 0
2 6 0 .0 0
2 8 0 .0 0
3 0 0 .0 0
纳米
3 2 0 .0 0
3 4 0 .0 0
3 6 0 .0 0
3 8 0 .0 0
nm
(a)
1 .6 0
1 .4 0
234.4
1 .2 0
au
au
1 .0 0
0 .8 0
0 .6 0
0 .4 0
0 .2 0
371.8
0 .0 0
2 2 0 .0 0
2 4 0 .0 0
2 6 0 .0 0
2 8 0 .0 0
3 0 0 .0 0
纳米
3 2 0 .0 0
3 4 0 .0 0
3 6 0 .0 0
3 8 0 .0 0
nm
(b)
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Fig. 2. A single peak was identified at 6.83 min by UV detection (210 nm).
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Fig. 3. A major peak with high height and large area was identified at 6.83 min under total ion current
(TIC, ESI+) scan condition.
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