NATIONAL INSTITUTE OF TECHNOLOGY, ARUNACHAL PRADESH, YUPIA, Pin-791112
(Established by MHRD, Govt. of India)
Website : www.nitap.in, Fax No: (0360) 2284972
E-Mail: nitarunachal@gmail.com / admin@nitap.in
DEPARTMENT OF BIOTECHNOLOGY & CHEMICAL ENGINEERING
Exercises Proposed for teaching in (Jan – June, 2016 Semester)
1.
2.
3.
4.
Name of the Teacher:
Department:
Course Title:
Course code:
Dr. Pallabi Kalita Hui
Biotechnology & Chemical Engineering
Plants Cell and Tissue Culture
BT- 403
5. Course Hands out (in reference to framed & approved syllabus) (maximum 500 words)
Contents
Class estimate
Unit I:
Plant genomes and plant tissue culture:
Introduction-gene structure and gene expression-regulation,
12 classes
implication
for
plant
transformation,-Protein
targeting,
heterologous promoters, genome size and organization,
Arabidopsis and new technologies. Plant tissue culture plasticity
and totipotency, culture environment, growth regulators, media
regulators, culture types, plant regeneration.
8 classes
Unit II:
Plant transformation techniques:
Introduction- Agrobacterium mediated gene transfer –Ti-plasmidprocess of T-DNA transfer and integration, transformation in plant,
Direct gene transfer methods. Binary vectors- basic features of
vectors-optimization-clean gene technology.
8 classes
Unit III:
Transgenic plants-herbicide and pest resistance:
Herbicide resistance-use of herbicide in modern agriculturestrategies for engineering herbicide Resistance environment
impact, pest resistance-nature and scale of insect / pest damage to
crop GM strategies-Bt approach to insect resistance-copy nature
strategy-insect resistant crops and food safety.
12 classes
Unit IV:
Plant disease resistance and stress tolerance:
Introduction-plant-pathogen interactions-natural disease resistance
pathways-biotechnological - Approaches to disease resistance.
Plant viruses- types-entry and replication-transgenic approach
PDR Stress tolerance-abiotic stress-water deficit stress and various
approaches for tolerance. Introduction-carbohydrates and lipids
production-molecular farming of proteins-economic considerations
for molecular farming.GM crops-current status-concerns about
GM crops regulations of GM crops and products-Greener genetic
engineering.
NATIONAL INSTITUTE OF TECHNOLOGY, ARUNACHAL PRADESH, YUPIA, Pin-791112
(Established by MHRD, Govt. of India)
Website : www.nitap.in, Fax No: (0360) 2284972
E-Mail: nitarunachal@gmail.com / admin@nitap.in
DEPARTMENT OF BIOTECHNOLOGY & CHEMICAL ENGINEERING
6. Books/Literature to be followed:
(a) Books (Min. 2 texts + 3 references)
(i)
(ii)
(iii)
Title
Author
Publisher
Title
Author
Publisher
Year
Title
Author
Publisher
Year
Plant Biotechnology - The genetic manipulation of plants
Adrian Slater, NigelW.Scott and Mark R.Fowler
Oxford university press
Plant Cell and Tissue Culture
Indra K. Vasil, Trevor A. Thorpe
Springer Netherlands
1994
Practical Book of Biotechnology & Plant Tissue Culture Paperback
Madhavi Adhav , Santosh Nagar
Amazon
2010
.
(b) Magazines/Journals (Minimum 5)
1. Plant Cell, Tissue and Organ Culture (PCTOC) Springer
2. Plant Biotechnology Reports, Springer
3. Plant tissue culture & Biotechnology, Dhaka : Bangladesh Association for Plant Tissue
Culture & Biotechnology
4. Plant tissue culture: A history, Roger J. Gautheret, Springer
5. In Vitro Cellular & Developmental Biology - Plant Journal Impact Factor & Information,
Publisher: Tissue Culture Association; Society for In Vitro Biology, Society for In-Vitro
Biology
7. Mode of Teaching: J.C Bose√ / S. N. Bose (please tick).
8. If the course is of practices, list the experiments to be offered.
(i)
Sterilizing technique in plant tissue culture
(ii)
Preparation of culture media
(iii) Callus induction
(iv)
Micro propagation with shoot tip culture
(v)
Isolation of protoplast
Are the manuals ready for the experiments to be conducted?
Yes√ /No (please tick)
If “No”, the reasons there of and fixing responsibility
__________________________________________
NATIONAL INSTITUTE OF TECHNOLOGY, ARUNACHAL PRADESH, YUPIA, Pin-791112
(Established by MHRD, Govt. of India)
Website : www.nitap.in, Fax No: (0360) 2284972
E-Mail: nitarunachal@gmail.com / admin@nitap.in
DEPARTMENT OF BIOTECHNOLOGY & CHEMICAL ENGINEERING
__________________________________________
__________________________________________
__________________________________________
Remarks/ Endorsement by the HoD
With his /her signature with date
Name of the Teacher: Dr. Pallabi Kalita Hui
Designation: Assistant Professor
Signature with Date: