A few LEGO AFM talking points for use by Nano Day Student Guides 2013 What is an AFM The atomic force microscope (AFM) was first demonstrated in 1986 to image the surface of a material with high spatial resolution. The AFM is the most popular family member from a broad class of instruments called Scanning Probe Microscopes (SPMs). SPMs generally use the atomic force between atoms on a sharp tip and atoms on the surface of a material to image the surface features of that material. Note the acronyms AFM (Atomic Force Microscope) and SPM (Scanning Probe Microscope) tend to be used interchangeably. The AFM was developed to solve a wide-spread need – the need to reliably image nanometer scale surface features on materials with high resolution. In the 1980s, existing optical and electron microscopes could not meet this need. Overview During NanoDays, the LEGO Scanning Probe Microscope (SPM) will start scanning once in the morning (~9 AM) and once in the 1 afternoon (~1 PM). When visitors arrive at the Birck Center, the LEGO SPM will be acquiring an image of a LEGO landscape. Depending on when a group arrives, the LEGO SPM image will be at different stages of completion. As a NanoDay guide, it is important that you clearly tell visitors what to note as the LEGO SPM goes about its job of imaging the LEGO landscape. Emphasize that the LEGO SPM is a model of a real instrument; but is different in many ways. You must help them understand the similarities and differences. What is an AFM used for and why is it necessary? Research-quality Scanning Probe Microscopes are used everywhere in the world to image objects with nanometer dimensions. Such features are way too small to be seen with conventional optical microscopes. The capabilities provided by Scanning Probe Microscopes were one of the prime reasons why Nanotechnology grew so rapidly in the late 1980s. It is amazing that modern science and technology have developed in the past 30 years to make functional “things” with nanometer scale dimensions – transistors, sensors, medical devices. Progress ALWAYS requires you to view what you’ve made. Research-quality Scanning Probe Microscopes serve this purpose. 2 Many of the images displayed around the Birck lobby were acquired at Purdue with real SPMs. A few of the images were even taken by the LEGO SPM! Suggested Topics for Discussion A. The scale of “nano;” (give a perspective) A nanometer is really small! Nano comes the Greek word νανοσ that means dwarf. A useful way to convey the small size of a nanometer is provided by the “meter-stick” poster that hangs on the side of the LEGO SPM exhibit. It tells how long it would take to walk a distance of 1 meter if you took nanometer-size steps. How is it possible to make things with nanometer dimensions (1 nanometer=0.000000001 m) when you really cannot see what you are making? Nanotechnology requires that you know the shapes and locations of things you have no way of actually “seeing.” The LEGO Scanning Probe Microscope (SPM) is a working model designed to help you understand how scientists have solved this very fundamental problem of imaging at the nanoscale. 3 B. The Purdue LEGO SPM Built in 2003-2004 by a group of undergraduates at Purdue for use in science museums and middle schools throughout Indiana. Was featured in 2005 exhibit at the Children’s Museum of Oak Ridge (Tennessee) Has been in the Birck Center at Purdue since ~2008. Majority of funds for the design and construction came from a NASA grant. There are ~6 research-grade Scanning Probe Microscopes used in nanoscale research in the Birck Center here at Purdue. C. It’s a Different type of “microscope”! Explain that the SPM is a different “kind” of microscope than those that might be familiar to kids: It does not use “light.” (Ask students if they might know why. The wavelength of visible light is way too big to “reflect” from nanometer-size objects) Use the LEGO SPM to define S (scanning), P (probe) and M (microscope). An SPM only tells us about the surface, not what is inside. When using an SPM, we can’t actually “see” anything; the data obtained is a representation (a map) generated by the computer. It looks a lot like a 3-dimensional graph. 4 Students tend to have confused meanings about the vocabulary. Ask them to think about what these words mean: Scan Probe Atom Lever Cantilever Periodic Force Clarify what these words mean using the LEGO SPM as a demo tool so students can follow the discussion. D. Features of the SPM The “model” LEGO SPM faithfully captures all the essential features of a research-grade SPM. 1. A sharp tip is moved across an unknown sample that has features that you want to measure. 2. The tip is attached to a cantilever and the deflections of the cantilever are monitored by a focused laser beam. 3. The distance between the sample and a sharp tip is decreased in a systematic way until the tip “feels” the sample. 4. From this information, an accurate 3-dimensional image of the unknown sample can be obtained. E. How the LEGO model SPM works The LEGO SPM acquires an image at a rate of ~10 data points per minute. Image acquisition is slowed down so you can see how the SPM actually works. 5 The instrument measures 48 “data points” in each direction. Explain that a data point in a measurement of height at one specific point. What we see is a representation of all those data points. An entire image of the LEGO landscape requires ~ 3 hours and has ~2300 data points. The LEGO SPM is designed to image LEGO landscapes with overall dimensions of 15 inches x 15 inches. As you can see, the surface under study today has features made from stacked LEGO bricks. The features appear in a periodic (repeating) pattern, in much the same way as atoms are arranged on the surface of a crystalline solid. The computer software interprets the probe position at the point of contact to create a color map of the surface of our sample on the monitor (look at the image in the red box below). Different colors represent different heights. The image is updated every few seconds. Imagine you put a blanket over the entire LEGO SPM, so you are unable to directly observe the LEGO landscape by eye. If you look at the computer image of the LEGO landscape, what conclusions might you make about the actual LEGO landscape? ANSWER: After careful study of the entire LEGO SPM image, you might surmise the LEGO landscape is comprised of localized features arranged in a 6 x 6 periodic pattern. 6 Ask students “What changes do you think you could make to the LEGO SPM to improve the image?” POSSIBLE ANSWERS: use a sharper tip, make it go faster, take smaller steps so the image will have more data points. F. Additional Information Real SPMs acquire an image once every few minutes. Real SPMs map areas with dimensions that are about 1 million times smaller than the LEGO landscape that you see. R. Reifenberger Birck Center Purdue University April 17, 2013 7