SCREENING OF KDML 105 (Oryza sativa) MUTANTS BY RAPD ANALYSIS AND
SALT STRESS TOLERANCE
Jyotsna Joshi*, Kanyaratt Supaibulwatana#
Department of Biotechnology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok
10400, Thailand.
*email: jyotsna.joshi7@gmail.com, #email: kanyaratt.sup@mahidol.ac.th
Abstract
Soil salinity, regarded as one of the major abiotic stresses that limits the productivity of crop
species. This investigation is aim to determine the overview genetic variations in response to salt
tolerance among the mutants of Khao Dwak Mali105 (KDML 105). For evaluation of the genetic
mutation, Random amplified polymorphic DNA (RAPD) analysis was used to detect the genetic
variation among the putative mutants to their original KDML105. Among several series of
Operon kit, the operon primers OPA, OPB, OPC, OPJ, OPK, OPL, and OPW were selected due
to their potential to screen the different among control and mutants. Multiple 10 base pair (bp)
oligonucleotide primers were used each to an individual sample of DNA which was then
subjected to PCR. The resulting amplified DNA markers revealed random polymorphic segments
with band sizes from 100 to 3000 bp depending upon the genomic DNA and the primer. Among
116 primers used in this study, 31 primers showed several bands. The appropriate primers were
selected based on the range of the marker size. These appropriate primers can be used as
reference patterns of the control plant and to compare with the mutants. RAPD analysis was used
to identify mutants that showed tolerant ability to 150 mM NaCl stress after screening in
hydroponic system and showed different scores of salt tolerance.
Keywords: RAPD, heterozygosity, KDML 105
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