An-najah National University
Faculty of medicine and health sciences
Course specifications
Basic Information
Program on which the course is given: Bachelor of pharmacy
Department offering the programme: Department of Pharmacy
Department offering the course: Pharmaceutical chemistry and natural products division
Academic year/level: 2011(Forth year- First Sem)
Course title: Instrumental analysis
Course code: 7303303
Course Credit hours: 4 hours
Prerequisites: analytical chemistry (74004004).
Time and location:19G100
Instructor:
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Name: Murad Abualhasan, B.Pharm, Msc (Clin Epi), Msc (Pharm analysis), PhD
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Phone: 0595472322
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Email: M_abualhasan@najah.edu
Professional information:
Course Description:
Instrumental analysis course will
explain the theory of operation, instrumental design,
methodology, and applications of instrumental techniques of spectroscopic methods including
infrared, UV/VIS, MS, and chromatographic methods including gas, liquid, and thin layer
chromatography and some potentiometric and coulometric methods of analysis.
Course objectives:
At the end of the course the student will have gained of the followings:

The theory of operation, instrumental design and application of various
chromatographic methods

The theory of operation, instrumental design and application of various spectroscopic
methods

The theory of operation, instrumental design and application of various
Potentiometric, Voltammetric and coulometric methods
Intended learning outcomes (ILOs):
a. Knowledge and understanding:
a.1
Recognise the interactions of the electromagantic radiation with drug atom or molecule
a.2
Describe the instrumental principles involved in the atomic absorption and emission
a.3
Explain the principles of molecular absorption and fluorescence.
a.4
Explain the principles involved in UV/Vis spectrophotmetry
a.5
Recognise the pharmaceutical application of different spectrophotmetric instruments
a.6
Describe the function of some specific parts of the instrument
a.7
Describe different chromatographic separation technique
a.8
Explain the different potentiometric and coulometric instruments
b. Intellectual Skills:
b.1
Differentiate between absorption and emission involved in the atomic
spectrophotometry
b.2
Differentiate between UV/visible and spectrofluorometry.
b.3
Interpretate the advantages and the limitation of spectrophotmetric and
chromatographic instrumentation
b.4
Differentiate between direct calibration and standard addition method
c. Professional and practical skills:
c.1
Propose the most suitable instrument for analysing drugs
c.2
Develop certain drug analytical method based on the compounds structure
c.3
Determine the percentage assay of the drug in different pharmaceutical formulation
based on the result of the instrumental analysis.
c.4
Evaluate the instrumental application in the international pharmacopeia.
d. General and transferable skills:
d.1
Search the internet database of up to date pharmaceutical instrumentation
d.2
Independent learning through literature review and presenting the work
d.3
Appreciate team work in the seminar
d.4
Work in a team
d.5
Respect assisting personal.
Course outline and calendar:
Topics
Chapter I: Introduction to instrumental analysis
Week/ hours
1 hours
Chapter II: Spectrophotometric instrumentation

In troduction to Spectrophotometry

Electromagnetic Radiation and energy

Atomic emission spectrophotometry

Atomic Absorption Spectrophotometry

Ultraviolet /visible spectrophorometry

Infra Red spectrophotometry

Near Infrared Spectroscopy (NIRS)

Spectropolarimetry

Fluorescence spectrophotometry
11 hours
First hour exam
Chapter III: Chromatographic instrumentation

Introduction to chromatographic techniques

High Pressure liquid Chromatography (HPLC)
o Chromatographic peaks produced by a HPLC
o Stationary and Mobile Phases used in the HPLC
o HPLC Analysis of Acids and Bases
o Quantitative HPLC
12 hours

Normal phase chromatography

Reverse phase chromatography

Ion chromatography

Chiral chromatography

Thin layer chromatography

Gas chromatography
Second hour exam
Chapter IV: Other instrumentation commonly used in the
pharmaceutical application

Mass spectrometry
8 hours

Capillary electrophoresis

pH meter

Water Determination. (Karl Fischer Method).

NMR
Final Exam
32 hours
Total hours
Teaching and learning methods:
Tool
Lectures
ILOs
a.1, a.2, a.3, a.4, a.5, a.6, a.7, a.8.
Small group discussion sessions and seminars
d.4, d.5
Micro-project (Review articles)
d.1 , d.2, a8
Intellectual and practical skills
b.1, b.2, b.3, b.4, b.5, c.1, c.2, c.3, c.4
Text Books:
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Pharmaceutical Analysis, David G. Watson, 2004
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Principles of Instrumental Analysis Douglas A. Skoog, 5th Edition
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Modern Instrumentation Methods and Techniques, Francis Rouessac and Annick
Rouessac, Second Edition.
References:
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Published scientific papers
Additional material or equipments needed for the course:
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Internet sources
Supplementary Material:
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Reviews and articles from the international journals on the web
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The references in the library of the university