Supplementary information
Supplementary Table 1 The scoring class in G. boninense disease
Scoring class Disease symptoms
1
2
3
4
5
6
Healthy plants
Colonized plant root surface with G. boninense
Appearance of white fungal mass, with or without chlorotic leaves
Appearance of basidiomata with 1-3 chlorotic leaves
Formation of basidiomata with > 3 chlorotic leaves
Formation of well-developed basidiomata and the plants dried
Supplementary Table 2 Treatments for experiments
Treatments Description
T1 (G)
T2 (GU)
G. boninense PER 71 inoculated (+ plant + carrier + fungi)
G. boninense PER 71 uninoculated (+ plant + carrier – fungi)
T3 (GPC)*
T4 (T)
T5 (TU)
T6 (NC)
G. boninense PER 71 inoculated carriers without the plant (+ carrier + fungi – plant)
T. harzianum FA 1132 inoculated (+ plant + carrier + fungi)
T. harzianum FA 1132 uninoculated (+ plant + carrier – fungi)
Untreated negative controls (absolute control)
*T3 (GPC) was used to verify G. boninense.
1

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Time points (day)
42 63
ACC
KAS II
OTE
ACT
PTE
KAS III
(b)
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ACT
PTE
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Time points (day)
42 63
Supplementary Fig. 1 Expression of FA biosynthetic pathway genes (a) in leaf and (b) root tissues in untreated negative control plant over a time points of 63 days.
Values are means ± S.E. (n =15). Different letters indicate significant differences at each time points at P ≤ 0.0001
2

Supplementary Fig. 2 Expression profile of genes associated with FA biosynthetic pathway in leaf tissues during oil palmG. boninense interaction at 0 to 63 dpi.
ACC , acetyl-CoA carboxylase; KAS II , β-ketoacyl-ACP synthases
II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase;
KAS III, β-ketoacyl-ACP synthases III; Ac , actin; M, Mass marker; NTC, PCR negative control
Supplementary Fig. 3 Expression profile of genes associated with FA biosynthetic pathway in root tissues during oil palmG. boninense interaction at 0 to 63 dpi. ACC , acetyl-CoA carboxylase; KAS II
, β-ketoacyl-ACP synthases
II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase;
KAS III, β-ketoacyl-ACP synthases III; Ac , actin; M, Mass marker; NTC, PCR negative control
3

Supplementary Fig. 4 Expression profile of genes associated with FA biosynthetic pathway in leaf tissues during oil palmT. harzianum interaction at 0 to 63 dpi. ACC , acetyl-CoA carboxylase; KAS II , β-ketoacyl-ACP synthases
II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase;
KAS III, β-ketoacyl-ACP synthases III; Ac , actin; M, Mass marker; NTC, PCR negative control
Supplementary Fig. 5 Expression profile of genes associated with FA biosynthetic pathway in root tissues during oil palmT. harzianum interaction at 0 to 63 dpi. ACC , acetyl-CoA carboxylase; KAS II
, β-ketoacyl-ACP synthases
II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase;
KAS III, β-ketoacyl-ACP synthases III; Ac , actin; M, Mass marker; NTC, PCR negative control
4

(a)
1,00
0,90
0,80
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0,60
0,50
0,40
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0,10
0,00
a b b b c d
ACC
b b b
a b b b
a
b b b
a
KASII
c
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OTE ACT
FA biosynthetic pathway genes b b bc
a
PTE d e b b b a
KASIII
c
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0
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63
(b)
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0,90
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63
0
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7
ACC KASII OTE ACT
FA biosynthetic pathway genes
PTE KASIII
Supplementary Fig. 6 Quantification of expression of FA biosynthetic pathway genes in oil palm leaf tissues at 0 to
63 dpi during interactions with G. boninense. (a) G. boninense inoculated; (b) G. boninense uninoculated. (c)
Untreated negative control. ACC , acetyl-CoA carboxylase; KAS II , β-ketoacyl-ACP synthases II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase; KAS III , β-ketoacyl-
ACP synthases III.
Data presented as mean ± S.E of three biological replicates and three technical replicates. Different letters indicate significant differences in each time points at P ≤ 0.0001
5

(c)
1,00
0,90
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0,60
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ACC KASII OTE ACT
FA biosynthetic pathway genes
PTE
Supplementary Fig. 6 continued
KASIII
0
3
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42
63
6

(b) 1,00
0,90
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ACC
b ab a a a a a a a a b b aba ab c c c c
b ab aab a
KASII OTE ACT
FA biosynthetic pathway genes
PTE d c bcbc aab
KASIII
0
3
7
21
42
63
21
42
63
0
3
7
ACC KASII OTE ACT
FA biosynthetic pathway genes
PTE KASIII
Supplementary Fig. 7 Quantification of expression of FA biosynthetic pathway genes in oil palm leaf tissues at 0 to
63 dpi during interactions with T. harzianum. (a) T. harzianum inoculated. (b) T. harzianum uninoculated. (c)
Untreated negative control. ACC , acetyl-CoA carboxylase; KAS II , β-ketoacyl-ACP synthases II; OTE , oleoyl-ACP thioesterase; ACT , glycerol-3-phosphate acyltransferase; PTE , palmitoyl-ACP thioesterase; KAS III , β-ketoacyl-ACP synthases III.
Data presented as mean ± S.E of three biological replicates and three technical replicates. Different letters indicate significant differences in each time points at P ≤ 0.0001
7

(c)
1,00
0,40
0,30
0,20
0,10
0,00
0,90
0,80
0,70
0,60
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ACC KASII OTE ACT
FA biosynthetic pathway genes
PTE
Supplementary Fig. 7 continued
KASIII
0
3
7
21
42
63
8