DNA affinity purification assay (DAPA). DNA precipitations were carried out essentially as described previously.
1
Briefly, cell extracts (500
 g) were precleared with Streptavidin-agarose beads (Sigma) for 30 min at 4 º C, then incubated with 4
 g of biotinylated double-stranded oligonucleotides corresponding to the regulatory element of the CDH1 promoter, together with 2
 g of poly(dI-dC) for 16 h at 4 º C.
DNA-bound proteins were collected with Streptavidin-agarose beads for 2 h and analyzed by Western blotting. 5’-biotinated sequences were:
(5’-Biotin-GGCTGTGGCCGGCAGGTGACCCCTCAGCCAATCAGCGGTACGGG
GGGCGGTGCTCCGGGGCTCACCTGGCTGCAGCCA-3’)
References:
1.
Hata A., Seoane J., Lagna G., Montalvo E., Hemmati-Brivanlou A., Massagué J.
OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and
Olf signaling pathways. Cell . 2000;100:229–240 .

Figure S1. Examples of representative G9a immunohistochemical (IHC) staining in endometrial cancer tissues ( left column ) and normal paired tissues ( right column ) from tissue arrays. A strong G9a nuclear staining for endometrial cancer tissues is shown in the magnifier.
Figure S2. Effects of G9a on cell survival and invasiveness in endometrial cancer cells . (a), Western blot analysis of G9a, GLP, and H3K9me2 in various endometrial cancer cell lines. (b), Top , G9a protein expression was examined by Western blotting in RL95-2 stably expressing a control shRNA or G9a shRNA. Bottom , The cellular growth and survival of RL95-2 cells expressing G9a shRNA. * P < 0.01 relative to the control cells. (c), Individual cell migration was monitored by time-lapse microscopy.
Overlays of representative trajectories (n = 5) are shown for the control cells and
G9a -knockdown cells (RL95-2). (d), The average distance traveled was calculated by tracking the individual cell movement of 30 cells per condition. Three independent experiments were performed with similar results.
Figure S3. Knockdown of G9a expression induces apoptosis of endometrial cancer cells. Cells were stained with Annexin V-FITC and propidium iodide (PI), followed by analysis on a flow cytometer. Columns , the mean of three independent experiments; bars , SD. * P < 0.01.

Figure S4. Western blot analysis results for G9a and E-cadherin expression in RL95-2 cells and G9a-knockdown RL95-2 cells.
Figure S5. The binding of Snail, DNMT1 or G9a to the biotinylated putative regulatory element of CDH1 promoter was performed by DAPA and analyzed by
Western blot.