4.2 (c) Post-experiment questions- Prepare and examine a plant cell 1. Name the parts on the diagram of the light microscope. A _________________________________ B _________________________________ C _________________________________ D _________________________________ E _________________________________ F _________________________________ 2. Give the function of the parts E__________________________________________________________________ F __________________________________________________________________ B __________________________________________________________________ 3. If the structure labeled A is marked X 10 and the structure labeled D is marked X 40 what is the total magnification of the object? ___________________________________________________________________ ___________________________________________________________________ 4. Describe how you prepared a plant cell for examination under the microscope. Include details of the stain used and why. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ Biology SLSS 2009 1 5. Why is it advised to observe cells under low power first? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 6. Describe how you prepared an animal cell for examination under the microscope. Include details of the stain used and why. ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 7. All cells are small. A microscope is therefore needed to observe the detailed structure of cells. Small cell size is essential for a high rate of metabolism. Explain the underlined term. ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 8. Name the kingdom whose members do not have a nucleus in their cells. ______________________________________________________________________ 9. You are given two slides, one with plant and one animal tissue. (a) Using labeled diagrams show the procedure used to complete the preparation of the two stained slides for viewing under the microscope. Plant cell Biology SLSS 2009 Animal Cell 2 (b)Give one safety precaution followed during this investigation. _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ 10. (a) If making a plant cell slide to view under the microscope state the source of the plant cells. _________________________________________________________________ _________________________________________________________________ (b) From exactly what location in the plant are the cells extracted? _________________________________________________________________ (c) Outline how you used the microscope to view the slide. _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ _________________________________________________________________ (d) Draw a labelled diagram to show the results obtained. (e) How do you ensure that the lenses are in place properly? _________________________________________________________________ _________________________________________________________________ (f)What holds the slide in place on the stage? _________________________________________________________________ Biology SLSS 2009 3 Biology SLSS 2009 4