OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Campylobacteriosis (including Bovine Genital
Campylobacteriosis and C. jejuni / C. coli)
Address of laboratory
Utrecht University, Faculty of Veterinary Medicine,
PO box 80.165, 3508 TD Utrecht, the Netherlands
Central Veterinary Institute, PO box 65, 8200 AB
Lelystad, the Netherlands
Tel.:
Utrecht : +31-30-2531242
Lelystad : +31-320-238157
Fax:
Utrecht: +31-30-2533199
Lelystad: +31-320-238961
e-mail address:
j.wagenaar@uu.nl
website:
http://www.uu.nl/faculty/veterinarymedicine/nl/
Name (including Title) of Head of
Laboratory (Responsible Official):
Prof. Jaap A. Wagenaar, DVM, PhD
Name (including Title and Position) of OIE
Reference Expert:
Prof. Jaap A. Wagenaar, DVM, PhD
Date of submission to the OIE
February 19th, 2013
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of
January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE
should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference
Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute
with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
Biochemical testing
10
Direct diagnostic tests
Nationally
Internationally
Amplified Fragment Length Polymorphism (AFLP)
650
150
PCR (Abril et al., 2007)
250
150
Multi Locus Sequence Typing (MLST)
10
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
4.
No
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
2
No
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No
Name of OIE
Member Country
seeking assistance
Date
United Kingdom
February and
October
9 and 5
Argentina
February, July
and August
12, 24 and 23
New-Caledonia
July
12
Bulgaria
November
2
Ethiopia
June and
October
87 and 40
Sudan
February and
October
19 and 16
10.
No. samples received for provision
of diagnostic support (i.e. from
surveillance campaign)
No. samples received for
provision of confirmatory
diagnoses
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
SVE, Sweden
Advice about labs performing ELISA test
Remote assistance, email
Coda – Cerva, Brussels, Belgium
Advice about available diagnostic tests
Remote assistance, email
Ethiopia
Advice about Campylobacter isolation
Remote assistance, email
Ahfad University for Women,
Omdurman, Sudan
Advice about Campylobacter isolation
Remote assistance, email
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
No
Title of the study
Duration
Purpose of the study
Whole genome
sequencing of
Campylobacter fetus
subspecies
ongoing
Whole genome
sequencing of
Campylobacter fetus
subspecies
Annual reports of OIE Reference Laboratories, 2012
Partners
(Institutions)
OIE Member Countries
involved other than
your country
USDA
USA
3
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
Infection trial of
Campylobacter fetus
subspecies in cattle
ongoing
Pathogenicity of
Campylobacter
subspecies in cattle
INTA
Argentina
Studies on
pathogenicity
ongoing
Molecular
characterisation of
strains
University of Graz
Austria
CamVac
ongoing
Vaccination against
Campylobacter jejuni
Danish Technical
University (DTU)
as projectleader
(Danish Research
Council)
Denmark, US, Indonesia
CamCon
ongoing
Vaccination against
Campylobacter jejuni
and phage therapy to
combat C. jejuni
EU-Consortium
(led by the
Norwegian
Zoonoses Center)
Norway, Denmark, UK,
Spain, Poland, Portugal
Campylobacter in
the poultry meat
production chain
ongoing
Descriptive
epidemiology of
Campylobacter in the
poultry production
chain in Sri Lanka
Vet School in
Peradeniya
Sri Lanka
WHO Global Food
Borne Infections
Network (WHO-GSS)
ongoing
WHO-Capacity
building program
Global
collaboration
Global collaboration
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 8
b)
International conferences: .............................................. 7
c)
National conferences: ...................................................... 2
d)
Other: ...............................................................................
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
4
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
a)
Technical visits: ................................................................ 1
b)
Seminars: ......................................................................... 2
c)
Hands-on training courses: .............................................. 0
d)
Internships (>1 month): ................................................... 0
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s) provided
with training
a
Sri Lanka (host), appr 15 participating
countries
b
Brazil (host), appr 50 participating countries
b
Japan (host) appr. 15 participating countries
No. participants from the
corresponding country
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
ISO 9001 and ISO 17025
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
No
National/
International
Title of event
Co-organiser
Date
(mm/yy)
Location
No.
Participants
international
OIE focal points Food
Safety
OIE, FAO, WHO
November
2012
Tokyo, Japan
40
international
WHO Expert Meeting on
Campylobacter
OIE, FAO
July 2012
Utrecht, The
Netherlands
30
20.
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Annual reports of OIE Reference Laboratories, 2012
No
5
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
Title of event
WHO Expert
meeting on
Campylobacter
Date
(mm/yy)
Location
Role (speaker, presenting
poster, short
communications)
Title of the work
presented
06/12
July 2012
Geneva WHO
Utrecht, NL
Expert and host/organiser
Control strategies for
Campylobacter
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
No
Purpose for inter-laboratory test comparisons1
No. participating
laboratories
Participating OIE Member
Countries
Proficiency testing for National Laboratories in NL
18
national
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
1
6
No
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
Kind of consultancy
Location
Subject (facultative )
Speaker at Focal Point Meeting Food
Safety
Tokyo, Japan
Campylobacter and Salmonella
Provision of comments on OIE Standards
Remote (e-mail)
Addition of a validated test to the
OIE Manual (BGC)
Attachment – Output associated with the Campylobacter Chapter (expert: JA Wagenaar))
Peer reviewed publications
1.
Friesema IH, Havelaar AH, Westra PP, Wagenaar JA, van Pelt W. 2012. Poultry Culling and
Campylobacteriosis Reduction among Humans, the Netherlands. Emerg Infect Dis. 18:466-468.
2.
van Bunnik BA, Katsma WE, Wagenaar JA, Jacobs-Reitsma WF, de Jong MC. 2012. Acidification of
drinking water inhibits indirect transmission, but not direct transmission of Campylobacter between
broilers. Prev Vet Med. 105(4):315-319.
3.
Louwen R, Nieuwenhuis EE, van Marrewijk L, Horst-Kreft D, de Ruiter L, Heikema AP, van Wamel WJ,
Wagenaar JA, Endtz HP, Samsom J, van Baarlen P, Akhmanova A, van Belkum A. 2012. Campylobacter
jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide
structures. Infect Immun. 80:3307-3318.
4.
Mughini Gras L, Smid JH, Wagenaar JA, de Boer AG, Havelaar AH, Friesema IH, French NP, Busani L,
van Pelt W. 2012. Risk factors for campylobacteriosis of chicken, ruminant, and environmental origin: a
combined case-control and source attribution analysis. PLoS One.7:e42599.
5.
Kalupahana RS, Kottawatta KS, Kanankege KS, van Bergen MA, Abeynayake P, Wagenaar JA. 2013.
Colonization of Campylobacter spp. in Broiler Chickens and Laying Hens Reared in Tropical Climates
with Low-Biosecurity Housing. Appl Environ Microbiol.79:393-395.
6.
Louwen R, Horst-Kreft D, de Boer AG, van der Graaf L, de Knegt G, Hamersma M, Heikema AP, Timms
AR, Jacobs BC, Wagenaar JA, Endtz HP, van der Oost J, Wells JM, Nieuwenhuis EE, van Vliet AH,
Willemsen PT, van Baarlen P, van Belkum A. 2013. A novel link between Campylobacter jejuni
bacteriophage defence, virulence and Guillain-Barré syndrome. Eur J Clin Microbiol Infect Dis.
32(2):207-26.
7.
Smid JH, Mughini Gras L, de Boer AG, French NP, Havelaar AH, Wagenaar JA, van Pelt W. 2013.
Practicalities of using non-local or non-recent multilocus sequence typing data for source attribution in
space and time of human campylobacteriosis. PLoS One. 8(2):e55029.
8.
Lapo Mughini Gras, Joost H. Smid, Jaap A. Wagenaar, Miriam G. Koene, Arie H. Havelaar, Ingrid H.M.
Friesema, Nigel P. French, Carolien Flemming, Caterina Graziani, Luca Busani, Wilfrid van Pelt
Increased Risk for Campylobacter jejuni/coli infection of Pet Origin in Dog Owners and Genetic Evidence
of Pet-Human Transmission. Epidemiol. Infect. Accepted for publication.
Annual reports of OIE Reference Laboratories, 2012
7
OIE RL for « Campylobacteriosis » – « Prof. Jaap A. Wagenaar » – « Netherlands »
Abstracts
1.
Maarten Gilbert, Linda van der Graaf–van Bloois, William G. Miller, Emma Yee, Birgitta Duim and Jaap
A. Wagenaar. CRISPR-Cas system in the genomes of Campylobacter fetus subspecies. The Scientific
Spring Meeting. Royal Dutch Society of Microbiology (KNMV) and the Dutch Society of Medical
Microbiology. Arnhem, the Netherlands. 17-18 april 2012. Ned Tijdsch Med Microbiol (2012) 19:S82-83.
2.
Linda van der Graaf – van Bloois, Gregor Gorkiewicz, William G. Miller, Jaap A. Wagenaar and Birgitta
Duim. Molecular characterization of the pathogenicity island in Campylobacter fetus subspecies. ASM
112th General Meeting June 16-19, 2012, San Francisco, US.
3.
Linda van der Graaf–van Bloois, William G. Miller, Emma Yee, Birgitta Duim and Jaap A. Wagenaar.
Whole genome sequencing of Campylobacter fetus subspecies. ASM 112th General Meeting June 16-19,
2012, San Francisco, US.
4.
Maarten J. Gilbert, William G. Miller, Emma Yee, Birgitta Duim, Andrew J. Lawson, Martin J. Blaser and
Jaap A. Wagenaar. Whole genome sequencing for taxonomic positioning of Campylobacter fetus
testudinum ssp. nov. ASM 112th General Meeting June 16-19, 2012, San Francisco, US.
5.
Regional Campylobacter outbreak investigation: combining epidemiology and microbiology. E.B.Fanoy,
R. Diepersloot, D.Ooms, M. De Rosa, I.H.M.Friesema, M.P.D. Deege, W.van Pelt, A. de Boer, J.A.
Wagenaar. ESCAIDE 2012. Edinburgh, 24-26 October 2012.
Lectures on invitation, expert meetings, workshops
Expert Workshop on EU protocol for harmonised monitoring of antimicrobial resistance in Salmonella and
Campylobacter from humans, ECDC, Stockholm. 29-30 May, 2012. Invited Expert. (Wagenaar)
WHO-Campylobacter Expert Consultation. Utrecht. 9-11 July 2012. Organiser and local host. (Wagenaar)
Campylobacter, a friend of poultry but an enemy of public health. World Poultry Congress XXIV, Salvador,
Brazil, August 8th, 2012. Invited Lecture. (Wagenaar)
Antimicrobial resistance: epidemiology, relation vet-human and the way forward. International Workshop on
the use of antimicrobials in livestock production and antimicrobial resistance in the Asia-Pacific region. 36th
Session of the Animal Production and Health Commission for Asia and the Pacific (APHCA). Negombo, Sri
Lanka, 20-24 October 2012, Keynote lecture. (Wagenaar)
Campylobacter: food safety aspects and interventions. Regional Seminar for the OIE National Focal Points on
Animal Production Food Safety. Tokyo, Japan. 31 October- 2 November 2012. Invited Lecture. (Wagenaar)
8
Annual reports of OIE Reference Centres, 2012