Electronic Supplementary Material for ‘TREC Based Newborn Screening for Severe Combined Immunodeficiency Disease: A Systematic Review’ Journal of Clinical Immunology Jet van der Spek, BSca Rolf H. H. Groenwold, MD, PhDb Mirjam van der Burg, PhDc Joris M. van Montfrans, MD, PhDa a Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht, Utrecht, The Netherlands b Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands c Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands Corresponding author: Joris M. van Montfrans, MD, PhD University Medical Center Utrecht Lundlaan 6, PO Box 85090, 3508 AB Utrecht, The Netherlands Tel: +31 (0)88 755 4003 Fax: +31 (0)88 755 5030 j.vanmontfrans@umcutrecht.n 1 1 ONLINE RESOURCE 1 2 3 Search strategy (conducted on June 20, 2014) 4 5 PubMed 6 7 #1 (17056 hits) 8 “Newborn 9 “Screening”[tiab]) OR (“Neonatal”[tiab] AND “Screening”[tiab]) OR “Guthrie card”[tiab] OR “Guthrie 10 test”[tiab] OR “Dried blood spot”[tiab] OR “Dried blood spots”[tiab] OR “T cell receptor excision 11 circle”[tiab] OR “T cell receptor excision circles”[tiab] OR “T-cell receptor excision circle”[tiab] OR “T- 12 cell receptor excision circles”[tiab] OR “TREC”[tiab] OR “TRECs”[tiab] OR “Kappa deleting 13 recombination excision circle”[tiab] OR “Kappa deleting recombination excision circles”[tiab] OR 14 “Kappa-deleting recombination excision circle”[tiab] OR “Kappa-deleting recombination excision 15 circles”[tiab] OR “KREC”[tiab] OR “KRECs”[tiab] OR “Neonatal screening”[MeSH] OR “Dried Blood 16 Spot Testing”[MeSH] screening”[tiab] OR “Neonatal screening”[tiab] OR (“Newborn”[tiab] AND 17 18 #2 (30605 hits) 19 “PID”[tiab] OR “primary immune deficiency”[tiab] OR “primary immune deficiencies”[tiab] OR 20 “primary immunodeficiency”[tiab] OR “primary immunodeficiencies”[tiab] OR “SCID”[tiab] OR 21 “Severe combined immunodeficiency”[tiab] OR “T cell lymphopenia”[tiab] OR “T cell 22 lymphopenias”[tiab] OR “T-cell lymphopenia”[tiab] OR “T-cell lymphopenias”[tiab] OR “XLA”[tiab] OR 23 “X linked agammaglobulinemia”[tiab] OR “X-linked agammaglobulinemia”[tiab] OR “22q11 deletion 24 syndrome”[tiab] OR “DiGeorge syndrome”[tiab] OR “ataxia telangiectasia”[tiab] OR “ataxia- 25 telangiectasia”[tiab] 26 syndrome”[tiab] OR “B cell lymphopenia”[tiab] OR “B cell lymphopenias”[tiab] OR “B-cell 27 lymphopenia”[tiab] OR “B cell lymphopenias”[tiab] OR “B cell maturation defect”[tiab] OR “B cell 28 maturation defects”[tiab] OR “B-cell maturation defect”[tiab] OR “B-cell maturation defects”[tiab] 29 OR 30 agammaglobulinemia”[Supplementary Concept] OR “22q11 Deletion Syndrome”[MeSH] OR 31 “DiGeorge Syndrome”[MeSH] OR “Ataxia Telangiectasia”[MeSH] OR “Nijmegen Breakage 32 Syndrome”[MeSH] “Severe OR “Nijmegen Combined Breakage Syndrome”[tiab] Immunodeficiency”[MeSH] OR OR “Nijmegen-breakage- “Bruton type 33 34 #3 (197 hits) 35 #1 AND #2 2 36 EMBASE 37 38 #1 (16584 hits) 39 ‘Newborn screening’:ti,ab OR ‘Neonatal screening’:ti,ab OR ‘Guthrie card’:ti,ab OR ‘Guthrie test’:ti,ab 40 OR ‘Dried blood spot’:ti,ab OR ‘Dried blood spots’:ti,ab OR ‘T cell receptor excision circle assay’:ti,ab 41 OR ‘T cell receptor excision circles’:ti,ab OR ‘T-cell receptor excision circle assay’:ti,ab OR ‘T-cell 42 receptor excision circles’:ti,ab OR TREC:ti,ab OR TRECs:ti,ab OR ‘Kappa deleting recombination 43 excision circle’:ti,ab OR ‘Kappa deleting recombination excision circles’:ti,ab OR ‘Kappa-deleting 44 recombination excision circle’:ti,ab OR ‘Kappa-deleting recombination excision circles’:ti,ab OR 45 KREC:ti,ab OR KRECs:ti,ab OR ‘Newborn screening’/exp OR ‘Dried Blood Spot Testing’/exp 46 47 #2 (42391 hits) 48 ‘PID’:ti,ab OR ‘primary immune deficiency’:ti,ab OR ‘primary immune deficiencies’:ti,ab OR ‘primary 49 immunodeficiency’:ti,ab OR ‘primary immunodeficiencies’:ti,ab OR ‘SCID’:ti,ab OR ‘Severe combined 50 immunodeficiency’:ti,ab OR ‘T cell lymphopenia’:ti,ab OR ‘T cell lymphopenias’:ti,ab OR ‘T-cell 51 lymphopenia’:ti,ab 52 agammaglobulinemia’:ti,ab 53 syndrome’:ti,ab OR ‘DiGeorge syndrome’:ti,ab OR ‘ataxia telangiectasia’:ti,ab OR ‘ataxia- 54 telangiectasia’:ti,ab OR ‘Nijmegen Breakage Syndrome’:ti,ab OR ‘Nijmegen-breakage-syndrome’:ti,ab 55 OR ‘B cell lymphopenia’:ti,ab OR ‘B cell lymphopenias’:ti,ab OR ‘B-cell lymphopenia’:ti,ab OR ‘B cell 56 lymphopenias’:ti,ab OR ‘B cell maturation defect’:ti,ab OR ‘B cell maturation defects’:ti,ab OR ‘B-cell 57 maturation 58 Immunodeficiency’/exp OR ‘X linked agammaglobulinemia’/exp OR ‘chromosome deletion 59 22q11’/exp OR ‘DiGeorge syndrome’/exp OR ‘Ataxia Telangiectasia’/exp OR ‘Nijmegen Breakage 60 Syndrome’/exp OR defect’:ti,ab ‘T-cell OR OR lymphopenias’:ti,ab ‘X-linked ‘B-cell OR ‘XLA’:ti,ab agammaglobulinemia’:ti,ab maturation defects’:ti,ab OR OR OR ‘X ‘22q11 ‘Severe linked deletion Combined 61 62 #3 (170 hits) 63 #1 AND #2 AND [embase]/lim NOT [medline]/lim 64 65 Cochrane 66 67 #1 (596 hits) 68 "Newborn screening" or "Neonatal screening" or ("Newborn" and "Screening") or ("Neonatal" and 69 "Screening") or "Guthrie card" or "Guthrie test" or "Dried blood spot" or "Dried blood spots" or "T 70 cell receptor excision circle" or "T cell receptor excision circles" or "T-cell receptor excision circle" or 3 71 "T-cell receptor excision circles" or "TREC" or "TRECs" or "Kappa deleting recombination excision 72 circle" or "Kappa deleting recombination excision circles" or "Kappa-deleting recombination excision 73 circle" or "Kappa-deleting recombination excision circles" or "KREC" or "KRECs":ti or "Newborn 74 screening" or "Neonatal screening" or ("Newborn" and "Screening") or ("Neonatal" and "Screening") 75 or "Guthrie card" or "Guthrie test" or "Dried blood spot" or "Dried blood spots" or "T cell receptor 76 excision circle" or "T cell receptor excision circles" or "T-cell receptor excision circle" or "T-cell 77 receptor excision circles" or "TREC" or "TRECs" or "Kappa deleting recombination excision circle" or 78 "Kappa deleting recombination excision circles" or "Kappa-deleting recombination excision circle" or 79 "Kappa-deleting recombination excision circles" or "KREC" or "KRECs":ab (Word variations have been 80 searched) 81 82 #2 (470 hits) 83 "PID" or "primary immune deficiency" or "primary immune deficiencies" or "primary 84 immunodeficiency" 85 immunodeficiency" or "T cell lymphopenia" or "T cell lymphopenias" or "T-cell lymphopenia" or "T- 86 cell lymphopenias" or "XLA" or "X linked agammaglobulinemia" or "X-linked agammaglobulinemia" or 87 "22q11 deletion syndrome" or "DiGeorge syndrome" or "ataxia telangiectasia" or "ataxia- 88 telangiectasia" or "Nijmegen Breakage Syndrome" or "Nijmegen-breakage-syndrome" or "B cell 89 lymphopenia" or "B cell lymphopenias" or "B-cell lymphopenia" or "B cell lymphopenias" or "B cell 90 maturation defect" or "B cell maturation defects" or "B-cell maturation defect" or "B-cell maturation 91 defects" or "Bruton type agammaglobulinemia":ti or "PID" or "primary immune deficiency" or 92 "primary immune deficiencies" or "primary immunodeficiency" or "primary immunodeficiencies" or 93 "SCID" or "Severe combined immunodeficiency" or "T cell lymphopenia" or "T cell lymphopenias" or 94 "T-cell lymphopenia" or "T-cell lymphopenias" or "XLA" or "X linked agammaglobulinemia" or "X- 95 linked agammaglobulinemia" or "22q11 deletion syndrome" or "DiGeorge syndrome" or "ataxia 96 telangiectasia" or "ataxia-telangiectasia" or "Nijmegen Breakage Syndrome" or "Nijmegen-breakage- 97 syndrome" or "B cell lymphopenia" or "B cell lymphopenias" or "B-cell lymphopenia" or "B cell 98 lymphopenias" or "B cell maturation defect" or "B cell maturation defects" or "B-cell maturation 99 defect" or "B-cell maturation defects" or "Bruton type agammaglobulinemia":ab (Word variations 100 or "primary immunodeficiencies" or "SCID" or "Severe combined have been searched) 101 102 #3 (3 hits) 103 #1 AND #2 104 4 105 ONLINE RESOURCE 2 106 107 Completeness of reporting assessment protocol for included studies 108 109 Assessment items per domain: 110 111 1) Methods 112 a. Describes TREC assay (incl. control gene) 113 b. Reports TREC unit clearly 114 c. Reports TREC cut-off value 115 d. Reports rationale for cut-off 116 2) Results 117 a. Reports number of re-tests precisely 118 b. Reports number of repeat DBSs precisely 119 c. Reports number of referrals precisely 120 d. Reports mean normal TREC content 121 e. Reports exact TREC content for newborns with TCL 122 f. 123 g. Reports genetic diagnoses of individual typical SCID patients with TREC content 124 h. Reports diagnosis of individual other TCL patients with TREC content 125 i. Reports how many newborns referred did were lost to diagnostic follow-up 126 j. Describes why newborns were lost to follow-up 127 Reports CD3 values for identified cases 3) Discussion 128 a. Discusses whether cases could have been missed 129 130 Assessment protocol 131 132 I. Case series were assessed on items 1a-d, 2d-g. Pilot cohort studies were assessed on items 133 1a-d, 2a-d. Other items were considered non applicable (NA). If only typical SCID patients 134 were tested item 2g was NA. If only other TCL patients were tested item 2f was NA. 135 Population-based cohort studies were assessed on all items. 136 137 II. All questions were answered with yes (X) or no (O). 138 5 139 140 III. If more than half of applicable items within methods, results or discussion was scored as ‘no’, the study was considered to have low completeness of reporting. 6 ONLINE RESOURCE 3 Table 5. Assessment of completeness of reporting in studies included in systematic review based on adjusted STARD criteria Methods Results TREC TREC TREC Rationale Nr. Nr. repeat Nr. Mean Exact CD3 values Genetic assay unit cut-off cut-off retests DBS referrals normal TREC diagnosis TREC content SCID content Case series Morinishi X X O O NA NA NA X X X X Borte X X X X NA NA NA O O O X La Marca X X X O NA NA NA O X O X Mallott X X X O NA NA NA O X X NA Somech X X X X NA NA NA X X X X Adams X X X X NA NA NA X X O O Audrain X X X X NA NA NA O X O X Lingman X X X X NA NA NA O X X NA Cohort studies Borte X X X X X X X O NA NA NA Adams X X X X X X X X NA NA NA Audrain X X X X X X X O NA NA NA Chien X X X X O O O X X X X Verbsky X X X X X X X O X X X Kwan (2013) X X X O O X X O X X X Kwan (2014) X X X O O O X O O O X Vogel X O X X X X X X X X X X, item reported; O, item not reported (for specification of individual items see Online Resource 2); NA, not applicable Diagnoses other TCL Nr. loss to follow-up Reason loss to followup Discussion Missed cases Overall comple tness NA X NA X NA NA NA X NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA high high high high high high high high NA NA NA X X X X X NA NA NA O O X O X NA NA NA O O X O X NA NA NA X X X X X high high high low high high low high 7