PHUSA PCR MIX
V1214.02
Description
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PHUSA PCR Mix is a preconditioned gel containing Taq DNA polymerase,
dNTPs with optimum concentration for one (1) reaction, packed ready to use in a
standard PCR tube.
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Shelf life : 1 month
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Delivered with 2 buffers to be used for 25l or 50l PCR condition:
For 25l reaction volume, use the PHUSA Mix Buffer-V25 (1X)
For 50l reaction volume, use the PHUSA Mix Buffer-V50 (1X)
General Protocol
Use the following table to make your final mix:
Components
25µL PCR volume
50µL PCR volume
PHUSA PCR Mix
5l
5l
PHUSA Mix Buffer-V25
15l
N/A
PHUSA Mix Buffer-V50
N/A
45l
(4 - 7l)
(4 - 7l)
(Adjust to your optimal primers
(Adjust to your optimal primers
& templates condition)
& templates condition)
25l
50l
Primer (F+R) mix + DNA template
Total Volume/1 reaction
Typical PCR program:
Temperature (°C)
Time
Number of Cycle
First denaturation
95°C
3-5 min
1
Denaturation
95°C
30 sec
30-40
Annealing
Ta
30 sec
Extension
72°C
1 min /kb
72°C
5 – 10 min
Complete PCR
product
1
* Ta: Annealing temperature depends on the designed primer pair
* Quality Testing of Phusa PCR Mix:
Quality of Phusa PCR Mix is evaluated by the comparison of PCR product formed from
Solution Master Mix (as Reference sample) and Phusa PCR Mix (Investigated Sample)
200bp
Reference Samples
Investigated Samples
Agarose gel electrophoresis Result of Quality Testing
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PHUSA PCR Mix can be used with your current PCR protocols without any limitation.
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Following is the general way to handle PHUSA PCR Mix: add to the Mix the
buffer, the DNA and primers, then place the PCR tube into the PCR machine
WITHOUT ANY VORTEXING prior running the PCR.
-
Once the PCR is done, the sample can be used in Agarose gel analysis as usual.
If it was conserved at 4-100C for a long period of time (more than 1h), a
preheating at 85oC for 5 minutes of the PCR sample is recommended before
analysis.
Storage Temperature recommended: 4oC – 30oC - Avoid direct sunlight exposure
WARNING: DO NOT KEEP AT FREEZING TEMPERATURE (below 0*C)
WARNING: DO NOT VORTEX