Supporting Methods
Methods S1
Genotying mutant alleles
For genotyping point-mutant alleles, the following primers were used:
Name
Primer
length PCR product
cut by
paps1-1
dCAPS
oSV126/166
210
mutant by EcoRI
pad4-1
CAPS
GTO295/296
452
WT by BsmFI
npr1-1
dCAPS
GTO181/182
286
WT by NlaIII
jar1-1
dCAPS
GTO158/159
130
WT by BglI
ap2-1
dCAPS
GTO23/24
309
mutant by Hpy188I
eds1-2
Indel
GTO293/294
WT 1359, mutant
420
For genotyping T-DNA insertion alleles, gene-specific primers (called LP and RP primer) that
flank the T-DNA insertion site, and a T-DNA right border primer (BP) were used. These are
listed below
Oligonucleotide Sequence
Description
name
oSV166
oSV126
GTO295
genotype paps1-1
genotype paps1-1
genotype pad4-1
TAATGCCCATCATTACTCCTGCGAAT
GCTTTGTTTGATTCCATAGC
AGATTCAATGGTACAAAGATCGTT
GTO296
GTO181
GTO182
GTO158
TCTCGCCTCATCCAACCACTCTT
ATAAGGCACTTGACTCGGATG
AGTGCGGTTCTACCTTCCAA
TTTCTCAGTGTGTGTGTTTTTGATCATCAGAT
GTO159
GTO293
CTGTTTCTGAAGGCAAAAGCAGTGCGAA
ATATTGTCCCTCGGATTATGCT
GTO294
oSV100
oSV91
oSV126
oSV78
oSV77
oSV79
oSV120
oSV121
GTO151
GTO152
ML437
oSV139
ML438
CTCCAAGCATCCCTTCTAATGT
TCTCGTACAATCCAACATCTTG
AGTGTCCAACTCTCCAAGTTTC
GCTTTGTTTGATTCCATAGC
TGGGACCTAGACATGCAACTAG
TGTGAAGTAAACTCAACCCAGAC
GGTCTTCTATCAATGGAATTG
ACATGGAGATGTTGAACTGCC
CCACTGTTCCACGTATATCAAAC
TTGAAACCTTCGAAATATAAG
GTGGTGAAGAACTTGAAAGA
TGGTTCACGTAGTGGGCCATCG
AACGTCCGCAATGTGTTATTAAGTTGTC
TTCATAACCAATCTCGATACAC
genotype pad4-1
genotype npr1-1
genotype npr1-1
genotype jar1-1
a35S::NPR1::GFP
genotype jar1-1
genotype eds1-2
genotype eds1-2
genotype paps1-2 LP
genotype paps1-2 RP
primer
genotype paps1-3 LP
primer
genotype paps1-3 RP
primer
genotype paps1-4 LP
primer
genotype paps1-4 RP
primer
genotype paps2-3 LP
primer
genotype paps2-3 RP
primer
genotype pad3-1 LP
primer
genotype pad3-1 RP
Primer
BP primer for SALKPrimer
BP primer for WsTDNA
BP primer for SAILTDNA
TDNA
qRT-PCR
Primers used for the different genes are listed below.
Gene Name
AGI code
PDF2
At1g13320
PR1
PR2
AT2G14610
AT3G57260
Sequence
fw
GCATTTCACTCCTCTGGCTAAG
rev
GGCACTTGGGTATGCAATATG
fw
TGTGCCAAAGTGAGGTGTAAC
rev
TGATGCTCCTTATTGAAATACTGATAC
fw
GGTGTCGGAGACCGGTTGGC
SID2
AT1G74710
AT5G62730
At1g09500
At5g24780
rev
CCCTGGCCTTCTCGGTGATCCA
fw
TGAAGCAACAACATCTCTACAGGCG
rev
CCCGAAAAGGCTCGGCCCAT
fw
TCTTCGCCGCTTCCTATAAC
rev
AACTCCATCATACCGGCTAGAG
fw
CTCCTACAGAAACAAGCCTTAGAG
rev
GACCTGCAAGGAATAACAAGTAAC
fw
AATGGGCTGATTTGGTTGAG
rev
GTGCCAAAACGGCTACAAAG
Phenotypic analysis, measurements of organ and cell sizes
Petals were dissected from the 6th to 15th flowers and used for measurements. For leaves, the
4th and 5th leaves of plants at the bolting stage or the entire rosette were taken for
measurements. To measure organ size, the organs were placed with forceps onto Sellotape. Once
all organs were collected, the tape was stuck onto a black Perspex sheet for petals or on a blank
white paper sheet for leaves. The organs were scanned with a resolution of 3600 dpi in greyscale
(8-bit, petals) or 1200 dpi colour image (leaves) using the HP Scanjet 4370. The organ size was
then measured using the Image Processing and Analysis in Java (ImageJ) software
(http://rsbweb.nih.gov/ij/).