Transgenic and Gene Targeting Core
Cryopreservation of Sperm or Fertilized Embryos
Investigator:
SDRC member
Submission Date:
CCTSI member
eRA Commons Name:
Cryopreservation Protocol:
Contact Person:
Experimental Protocol:
Contact Number:
Speedtype:
Contact email:
Service Needed: Please mark one
EMBRYO CRYO: TGTC recommends 8-12, proven, stud males for mating with either investigator
supplied females or wild type females. In order to consider a line properly cryopreserved, the transgenic
community recommends having 300-400 embryos frozen (TGTC freezes at the 2-cell stage). Depending on
background strain, transgenic line and efficiency of breeders, this may take multiple collection attempts to
achieve the desired number of embryos.
SPERM CRYO: TGTC requests 2 proven, male mice between the ages of 8 and 20 weeks to freeze a
total of 20 straws. We will thaw one straw to check for sperm viability; please note TGTC cannot guarantee a
successful IVF recovery at this time.
Location of Investigator’s Mice:
Location of mice:
RC1
RC2
Outside Location
Background Info:
Background Strain:
FVB
C57BL/6
Room #:
Institute Name:
129
Balb/c
Other
Embryo Cryo Info:
Male Construct/Gene Mutation
# of Males Provided and Zygosity
Female Construct/Gene Mutation
# of Females Provided and Zygosity
Construct name here
HOM #
HET #
WT #
Construct name here
HOM #
HET #
WT #
Sperm Cryo Info:
Male ID #
Male DOB
Male age
Donor male zygosity/phenotype/genotype/comment
For more details, please contact the TGTC at
makeamouse@ucdenver.edu