OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which
you are a designated OIE Reference
Laboratory:
Escherichia coli
Address of laboratory
Reference Laboratory for Escherichia coli (EcL)
Faculté de médecine vétérinaire
Université de Montréal
3200 Sicotte Street
Saint-Hyacinthe (Quebec)
CANADA J2S 2M2
Tel.:
+ 450.773.8521
Fax:
+ 450.778.8108
e-mail address:
john.morris.fairbrother@umontreal.ca
website:
www.ecl-lab.ca
www.apzec.ca
Name (including Title) of Head of
Laboratory (Responsible Official):
John M. FAIRBROTHER
Name (including Title and Position) of
OIE Reference Expert:
John M. FAIRBROTHER
Date of submission to the OIE
2013-01-24
Annual reports of OIE Reference Centres, 2012
Professor
Professor
1
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
N/A
N/A
Nationally
Internationally
173
195
Conventional PCR pathogenic E. coli for
sample testing (10 virulence genes)
2 381
148
Conventional PCR pathogenic E. coli for
isolate testing (10 virulence genes)
1 623
2 560
1 776
-
N/A
Direct diagnostic tests
Slide agglutation E. coli O serotyping
Radioactive gene probe pathogenic E. coli
(20 virulence genes)
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
Type of
reagent
available
Related
diagnostic
test
Produced
/stored
E. coli
reference
strains
Slide
agglutation
E. coli O
serotyping
Produced
and
stored
F4 (K88)
antiserum
2
Slide
agglutation
E. coli
serotyping
Produced
and
stored
No
Amount
supplied
nationally
Amount
supplied
internationally
Name of recipient OIE Member
Countries and of institutions
2 strains
Private laboratory, USA
25 strains
Private laboratories, Canada
32 ml
University of Guelph, ON, Canada
3 ml
Prairie Diagnostic Services, SK, Canada
2 ml
Manitoba Agriculture, Food and Rural
Initiatives, MB, Canada
8 ml
University of Saskatchewan, SK, Canada
9 ml
Ministère de l’Agriculture, des
Pêcheries et de l’Alimentation du
Québec, QC, Canada
Annual reports of OIE Reference Centres, 2012
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
Type of
reagent
available
Related
diagnostic
test
Produced
/stored
F5 (K99)
antiserum
Slide
agglutation
E. coli
serotyping
Produced
and
stored
4.
Amount
supplied
nationally
Amount
supplied
internationally
32 ml
University of Guelph, ON, Canada
44 ml
Prairie Diagnostic Services, SK, Canada
1 ml
Manitoba Agriculture, Food and Rural
Initiatives, MB, Canada
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
Name of recipient OIE Member
Countries and of institutions
No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
No
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
10.
No
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
National Veterinary Research and
Quarantine Service(NVRQS), South
Korea
Development of O serotyping for E. coli
Remote assistance
Monitoring and control of animal
pathogenic and zoonotic E. coli
In loco
University of Yangzhou, China
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
Title of the study
Monitoring of
potentially zoonotic E.
coli in chickens
Epidemiological study
of contagious diseases
in the dromedary and
development of
therapeutic strategies
based on VHH
fragments against
diarrhea in the
newborn dromedary
Monitoring and
characterisation of
foodborne E. coli
O157:H7 in abattoirs
Identification of
potentially pathogenic
E. coli and their
antimicrobial
resistance from
chickens on farm and
at slaughter in Senegal
Identification of
potentially pathogenic
E. coli and their
antimicrobial
resistance from pigs
and chickens on farm
and at slaughter in
Vietnam
Prevalence and
characterisation of
potentially pathogenic
E. coli in unpasteurised
cheese in Brazil
No
Duration
Purpose of the study
Partners
(Institutions)
OIE Member Countries
involved other than
your country
2 years
Monitoring of
potentially zoonotic
E. coli in chickens on
farms
Arizona State
University (ASU)
USA
1 to 3
years
Detection and
monitoring of the
presence of
pathogenic E. coli in
the newborn
dromedary
Institut des
Régions Arides
(IRA), Ministère de
l’Agriculture
Tunisia
2 years
Monitoring and
characterisation of
foodborne E. coli
O157:H7 in abattoirs
University of Sao
Paulo State
(UNESP)
Brazil
3 years
Characterisation of
virotype, serotype,
phylotype, and
antimicrobial
resistance of E. coli
isolates
École Inter-États
des Sciences et
Médecine
Vétérinaires
(EISMV)
Senegal
2 years
Characterisation of
virotype, serotype,
phylotype, and
antimicrobial
resistance of E. coli
isolates
National Institute
of Veterinary
Research (NIVR)
Vietnam
2 years
Characterisation of
virotype, serotype,
phylotype, and
antimicrobial
resistance of E. coli
isolates
University of Sao
Paulo State
(UNESP)
Brazil
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
4
Annual reports of OIE Reference Centres, 2012
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 3
b)
International conferences: .............................................. 0
c)
National conferences: ...................................................... 5
d)
Other: ............................................................................... Invited speaker: 3, Internet
List of publications in peer-reviewed journals
Byun JW, Jung BY, Kim HY, Fairbrother JM, Lee WK (2012). Real-time PCR for differentiation of F4 (K88)
variants (F4ab, F4ac, F4ad) of enterotoxigenic Escherichia coli from diarrhoeic piglets. The Veterinary
Journal 193: 593-594.
Côté JP, Berthiaume F, Houle S, Fairbrother JM, Dozois C, Mourez M (2012). Identification and evolution
mechanism of new alleles coding for the AIDA-I autotransporter of porcine pathogenic Escherichia coli.
Applied and Environmental Microbiology. 78: 4597-4605.
Delisle B, Calinescu C, Mateescu MA, Fairbrother JM, Nadeau E (2012). Oral immunization with F4 fimbriae
and CpG formulated with carboxymethyl starch enhances F4-specific mucosal immune response and
induces Th1 and Th2 cytokines in swine. Journal of Pharmacy & Pharmaceutical Sciences 15: 642-656.
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
No
a)
Technical visits: ................................................................ 1
b)
Seminars
c)
Hands-on training courses
d)
Internships (>1 month): ................................................... 4
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
a
Brazil
1
d
Brazil
2
d
Senegal
1
d
Tunisia
1
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
American Association of Veterinary Laboratory
Diagnosticians (AAVLD)
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
No
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
6
No
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Escherichia coli » – « John Morris FAIRBROTHER » – « Canada »
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
No
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
No
_______________
Annual reports of OIE Reference Laboratories, 2012
7