Fig. S1
Fig. S1 Phylogenetic tree of S-RBPs. The phylogenetic tree was made by ClastalW2 Phylogeny using default
parameters.
Fig. S2
Fig. S2 Salt stress response of S-RBP11 RNAi transgenic plants. (a) Schematic map of RNAi vector for the
suppression of S-RBP11 and expression analysis of S-RBP11 in T1 RNAi lines. GAPc was used as an internal
control. (b) and (c) Responses of WT and S-RBP11 RNAi T2 seedlings to 0, 130, or 140 mM NaCl. In (c), error
bars represent standard deviation (n = 24 plants).
Fig. S3
Fig. S3 Expression analysis of S-RBP11 under abiotic stresses using semi-quantitative RT-PCR. (a) Expression
of S-RBP11 under 300 mM NaCl treatment for 0, 1, 2, 4, or 8 h. (b) Expression of S-RBP11 under cold treatment
for 0, 1, 6, 12, or 24 h. (c) Expression of S-RBP11 under 10 μM treatment for 0, 1, 2, 4, or 8 h. In (a) to (c),
GAPc was used as an internal control. Similar results were obtained from at least two biological replicates, with
one shown here.
Table S1 Primers used in this study
Gene
35S enhancer
copy 1
35S enhancer
copy 2
35S enhancer
copy 3
35S enhancer
copy 4
Forward
Reverse
Purpose
5′-TATCTCGAGGATCCCCAACATGGTGGAGC-3′
5′-CGCGTCGACTAGATATCACATCAATCCAC-3′
Cloning
5′-TATGTCGACGATCCCCAACATGGTGGAGC-3′
5′-CGCAAGCTTTAGATATCACATCAATCCAC-3′
Cloning
5′-TATAAGCTTGATCCCCAACATGGTGGAGC-3′
5′-CGCGAATTCTAGATATCACATCAATCCAC-3′
Coning
5′-TATGAATTCGATCCCCAACATGGTGGAGC-3′
5′-CGCCTGCAGTAGATACACATCAATCCAC-3′
Coning
LB1
5′-ATGGTCATAGCTGTTTCCTGTGTGAAATTG-3′
TAIL-PCR
LB2
5′-AACCTGTCGTGCCAGCTGCATTAATGAATC-3′
TAIL-PCR
AD1
5′-NGTCGASWGANAWGAA-3′
TAIL-PCR
AD2
5′-TGWGNAGSANCASAGA-3′
TAIL-PCR
AD3
5′-AGWGNAGWANCAWAGG-3′
TAIL-PCR
AD4
5′-WGTGNAGWANCANAGA-3′
TAIL-PCR
LB-seq
5′-CATTAATGAATCGGCCAACG-3′
Sequencing
S-RBP11
5′-ATCGTTTTGCACTACAGAGC-3′
5′-CACGAAACCGAAACCTTTTG-3′
RD29A
5′-CCTGAAGTGATCGATGCACC-3′
5′-CAGTGGGTTTGGTGTAATCG-3′
COR15A
5′-AACGCAAGAAGTCGTTGATC-3′
5′-CGTTGAGGTCATCGAGGATG-3′
GAPc
5'-GTGTCCCAACCGTTGATGTC-3'
5'-TCCCTTGAGTTTGCCTTCGG-3'
S-RBP11
At5g06190
5'-AAATTTTAATGGCGGCGCTC-3'
5′-TACAGCTCGCGATCCAGATC-3′
5'-TGCGTAGTCCACAAATATCG-3'
5′-ATTATTCCTCTAGCCCTCAG-3′
Quantitative
RT-PCR
Quantitative
RT-PCR
Quantitative
RT-PCR
Quantitative
RT-PCR
Semi-quantitative
RT-PCR
Semi-quantitative
RT-PCR
Semi-quantitative
GAPc
5'-CACTTGAAGGGTGGTGCCAAG-3'
5'-CCTGTTGTCGCCAACGAAGTC-3'
Entire S-RBP11
5'-ATATCTAGAATGGCGGCGCTCGCA AGGAT-3'
5'-TATCCCGGGGTCGCTTTTCGATGTTTCGG-3'
Cloning
5'-CCGTCTAGAATGTCCGCCTCATGTTTCTTC-3'
5'-TATCCCGGGGTCGCTTTTCGATGTTTCGG-3'
Cloning
5'-ATCCTGCAGATGGTGAGCAAGGGCGAGGAG-3'
5'-ATCGTCGACCTTGTACAGCTCGTCCATGCC-3'
Cloning
5′-GAAGATCCAGCGAAAAGATGGAC
5′-GATCCAAAAAAACGGACAGCAATTAA
GGCTGTTCAAGAGACAGCCGTCCA
ACGGCCGGACGTCTCTTGAACGTCCGG
TCTTTTCGCTGGATCTTTTTTTG-3′
CCGTTTAATTGCTGTCCGTTCTGCA-3′
Truncated
S-RBP11
sGFP
S-RBP11 RNAi
RT-PCR
Cloning
Table S2 List of small RNA-binding proteins in Arabidopsis
S-RBP
Protein code
AGI code
aa length
Domain
S-RBP1
CAB78428, NP_193122
At4g13860
126
One RRM
S-RBP2
BAB09544, NP_200183
At5g53720
100
One RRM
S-RBP3
AAC61284, NP_179093
At2g14870
101
One RRM
S-RBP4
CAB88326, NP_190188
At3g46020
102
One RRM
S-RBP5
CAB78306, NP_193001
At4g12640
823
Two RRMs and SPOC domain
S-RBP6
AAC36547
S-RBP7
AAG51218 , NP_973958
At1g33470
245
One RRM
S-RBP8
AAD20390, NP_179762
At2g21690
117
One RRM
S-RBP9
AAC23648, NP_181287
At2g37510
142
One RRM
S-RBP10
AAF21210, NP_187457
At3g08000
143
One RRM
S-RBP11
BAB09686, NP_196239
At5g06210
146
One RRM
S-RBP12
BAB09337, NP_200269
At5g54580
156
One RRM
S-RBP13
BAB08354, NP_200794
At5g59860
157
One RRM
S-RBP14
AAB88654, NP_850366
At2g42240
292
Two RRMs
S-RBP15
BAB09540, NP_200179
At5g53680
169
One RRM
alcohol dehydrogenase, partial
(Fragaria viridis)