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SI Figure legends
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Fig. S1. Crossing for recombination analysis between Bm and Ws. Wild type
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females and F1 offspring (Bm × Ws) were crossed to obtain individuals with
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recombination between the Bm and Ws mutations.
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Fig. S2. Phenotypes of Bm and Ws mutations in pupae. Wild type (p50T),
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Bm mutant (No. 908), and Ws mutant (u42). The panels show the three
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different pupal stages: day 0, eye-pigmented stage, and 1 day before
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emergence. The phenotypes of both mutations were observed clearly from 1
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day before emergence. The arrowheads indicate the spot at the apex of the
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wing.
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Fig. S3. RT-PCR analysis of the candidate genes of Bm and Ws in the
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overlapping region. Twenty-four candidate genes were predicted based on
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the overlapping region (Fig. 4 and Table 3). The letters correspond to the
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numbers in Table 3. 18S ribosomal RNA was used as an internal control.
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Fig. S4. RT-PCR analysis of the candidate genes for Bm and Ws. RT-PCR
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was performed using primer sets based on the 5′- and 3′-UTRs using the
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sequences of full-length cDNA or EST clones that corresponded to the
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predicted genes: BGIBMGA005550 (I), 005522 (M), 005557 (S), and
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005656 (R) (Table 3). cDNA was prepared from the wing on pupal day 0. 1,
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p50T (wild type); 2, No. 908 (Bm mutant); 3, u42 (Ws mutant). The
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numbers on the right represent the fragment sizes of . 174 DNAs were
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digested separately with Hind III and Hae III. 18S ribosomal RNA was used
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as an internal control.
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