SUPPLEMENTAL MATERIALS
Table S1: Summary of the results of the multiple linear regressions examining the
relationship between microsatellite-based estimates of multilocus heterozygosity (MLH)
and offspring survival in Chinook salmon (Oncorhynchus tshawytscha). Regressions were
run separately for survival to the embryo, larvae, and fry developmental stages, and
included MLH, sex, and population as factors in the model. F-statistics (P-values) and
degrees of freedom (DF) for the effects of MLH on survival are indicated.
Developmental
Stage
DF
F (P)
Embryo
1
0.17 (0.68)
Larvae
Control
Translocation
1
1
0.02 (0.89)
0.38 (0.54)
Fry
Control
Translocation
1
1
0.30 (0.59)
0.05 (0.83)
Table S2: Summary of the results of the Monte Carlo simulations for tests of non-random
offspring survival with respect to nucleotide divergence (Dxy) across the entire MHC
molecule in two populations of Chinook salmon (Oncorhynchus tshawytscha). Observed
values for MHC class I and II Dxy that were greater than or less than the mean expected
value based on an assumption of random offspring survival are indicated respectively by
the + and - symbols. The p-value associated with the difference is shown in parentheses.
Control families remained in their natal environment whereas translocation families were
reared in the reciprocal hatchery.
Population Stage
Big
Qualicum Embryo
Quinsam
MHC class I Dxy
MHC class II Dxy
+ (0.001)
+ (0.09)
Larvae
Control
Translocation
+ (0.09)
+ (0.02)
+ (0.77)
+ (0.80)
Fry
Control
Translocation
+ (0.32)
+ (0.60)
+ (0.04)
+ (0.51)
+ (0.03)
+ (0.08)
Embryo
Larvae
Control
Translocation
+ (0.05)
+ (0.24)
- (0.0001)
+ (0.58)
Fry
Control
Translocation
+ (0.76)
+ (0.90)
- (0.09)
+ (0.27)
Figure S1: Summary of the Monte Carlo simulations for tests of non-random embryo
survival in two populations of Chinook salmon (Oncorhynchus tshawytscha). Results are
presented for the nucleotide divergence (Dxy) at the MHC class I and II loci. Data represent
the distributions of 1000 simulations for the expected value under random embryo survival
for (a) Big Qualicum MHC class I Dxy, (b) Quinsam MHC class I Dxy, (c) Big Qualicum
MHC class II Dxy, (d) Quinsam MHC class II Dxy. The vertical line in each panel indicates
the observed value.
Figure S2: Summary of the Monte Carlo simulations for tests of non-random larvae
survival in two populations of Chinook salmon (Oncorhynchus tshawytscha). Results are
presented for nucleotide divergence (Dxy) at the MHC class I and II loci. Data represent the
distributions of 1000 simulations for the expected value under random larvae survival for
(a) Big Qualicum control larvae MHC class I Dxy, (b) Big Qualicum translocation larvae
MHC class I Dxy, (c) Quinsam control larvae MHC class I Dxy, (d) Quinsam translocation
larvae MHC class I Dxy, (e) Big Qualicum control larvae MHC class II Dxy, (f) Big
Qualicum translocation larvae MHC class II Dxy, (g) Quinsam control larvae MHC class II
Dxy, (h) Quinsam translocation larvae MHC class II Dxy. The vertical line in each figure
indicates the observed value.
Figure S3: Summary of the Monte Carlo simulations for tests of non-random fry survival in
two populations of Chinook salmon (Oncorhynchus tshawytscha). Results are presented for
nucleotide divergence (Dxy) at the MHC II locus. Data represent the distributions of 1000
simulations for the expected value under random fry survival for (a) Big Qualicum control
fry MHC class II Dxy, (b) Big Qualicum translocation fry MHC class II Dxy, (c) Quinsam
control fry MHC class II Dxy, (d) Quinsam translocation fry MHC class II Dxy. The vertical
line in each figure indicates the observed value.