Genetic Variation of MHC Class II DRB3 gene in Local Goat from South
Sulawesi Indonesia
Muhammad Ihsan Andi Dagong1, Lellah Rahim1, Rr Sri Rachma Aprilita Bugiwaty1, & Nurul
Purnomo2
1
Animal Breeding and Genetic Laboratory, Animal Husbandry Faculty, Hasanuddin University,
Tamalanrea Makassar 90245, Indonesia, 2Integrated Biotechnology Laboratory, Animal Husbandry
Faculty, Hasanuddin University, Tamalanrea Makassar 90245
Abstract
Major Histocompatibility Complex (MHC) class II proteins are primarily restricted to the surface of
immune cells and are responsible for immune regulation. The objective of this study is to identify the
genetic variation of MHC class II DRB3 genes in Indonesian local goats from South Sulawesi
province. A total of 113 blood samples were collected from three local goats (Boerawa, Kacang and
Peranakan Ettawa) in South Sulawesi province. The genomic DNA was extracted by using Genomic
DNA extraction Kit and then MHC Class II DRB3 gene were amplified by PCR with predicted
amplicon length 285 bp. To identify alleles variation of MHC gene, the PCR product were cut with
HaeIII restriction enzymes. Genetic variation between populations calculated based on genotypic and
allelic frequencies, observed heterozygosity (Ho), expected heterozygosity (He) and the HardyWeinberg Equilibrium. There were 6 alleles found in local goat population (A, B, C, D, E and F
alleles). C and F were the common alleles found in this population (0.278 and 0.269 respectively),
while the rare allele was E allele (0.022). Genotype frequencies of this gene were respectively AA
(0.018), AB (0.053), BB (0.009), AC (0.142), BC (0.088), CC (0.106), AD (0.080), BD (0.018), CD
(0.009), DD (0.009), AE (0.018), BE (0.0), CE (0.009), DE (0.0), EE (0.0), AF (0.177), BF (0.053),
CF (0.097), DF (0.0), EF (0.018) and FF genotype (0.097). The observed heterozygosity value (Ho)
was 0.761 while the expected heterozygosity (He) was 0.771. Allelic variation found in local goat
population could be used as genetic information in selection program for disease resistant.
Keywords: Genetic Variation, MHC Class II DRB3, Local Goat, Disease Resistant, South Sulawesi