Supplementary Table 1: Sampled localities and genetic variability
Supplementary Table 2: Characteristics of 22 microsatellite loci for Drusus discolor
Supplementary Table 3: Genetic differentiation among sites (pairwise F
ST
and D Jost)
Supplementary Table 4: Variables used in SDM and their relative importance in the model
Supplementary Table 5: Correlation of the modified distances against Euclidian distance
Supplementary Table 6: Results of the multimodal inference model selection approach
1

Supplementary Table 1: Sampled localities and genetic variability within sites of Black Forest separated by headwater regions Feldberg (FB), Schauinsland (SL) and
Rohrhardsberg (RB), and Thuringian Forest separated by main drainage direction. Populations are numbered by 1) drainage and 2) by population number. ‘n’ = sample size; ‘F
IS
= inbreeding within a population; ‘A
R
’ = allelic richness; ‘H
O
’ = mean observed heterozygosity (SD). Deviations from Hardy Weinberg equilibrium are marked by ‘*’.
’
Region Population Catchment Longitude Latitude n F
IS
A
R
Gene diversity Ho
Black Forest
FB
RB
SL
FB01.1
FB02.1
FB02.2
FB02.3
FB03.1
FB03.2
FB03.3
FB03.4
FB04.1
FB04.2
FB05.1
FB05.2
FB05.3
FB06.1
SL07.1
SL07.2
SL07.3
SL08.1
SL08.2
SL09.1
SL10.1
RB11.1
RB12.1
RB12.2
RB12.3
RB13.1
RB13.2
S14.1
S15.1
S16.1
Sägenbach
Hauhensteiner Alb
Farnwittebach
Schwennbach
Schläglebach
Tiefkängelbach
Rotenbach
Rote Wiese tributary to St. Wilhelmer Talbach tributary to St. Wilhelmer Talbach tributary to St. Zastlerbach
Ödenbächle
Stollenbach
Schulterdobelbach
Tiefenbach
Haldenbächle
Schwarzenbach
Neumagen
Glashofbach
Langenbach
Reichenbach
Hirzbach
Glasbach
Obergefällbächle tributary to Haslachsimonswaldbach tributary to Yachbach tributary to Yachbach
Ferndobelbach
Sommerdobelbach
Steina
47.88228 24
47.85244 25
47.84369 24
47.82694 22
47.85208 25
47.84383 25
47.86106 23
47.85411 24
47.87667 24
47.87533 21
47.90231 24
47.91743 22
47.90630 24
47.92906 24
47.90791 24
47.89519 24
47.88167 23
47.89195 23
47.86383 24
47.91881 18
47.91810 24
48.12369 20
48.10186 24
48.12511 24
48.11914 24
48.14183 24
48.14506 24
47.99639 19
47.99008 24
47.81975 25
8.02439
8.03386
8.06878
8.02519
8.00186
8.00331
7.99078
7.97317
7.96778
7.98303
8.01861
8.00837
7.98940
8.00972
7.92096
7.91792
7.92347
7.86901
7.87001
7.87981
7.90126
8.15142
8.12897
8.12058
8.10722
8.13114
8.13294
8.15433
8.29897
8.24043
3.914
4.053
4.015
4.266
4.212
4.331
4.074
3.774
3.858
4.196
3.713
3.805
3.855
3.563
3.457
3.764
4.085
4.102
3.962
3.820
4.094
4.292
3.994
3.993
4.094
3.903
4.007
4.357
4.107
3.986
0.113
0.115
0.090
0.133
0.075
0.204
0.076
0.180
0.109
0.070
0.105
0.166
0.093
0.039
0.110
0.121
0.151
0.106
0.100
0.118
0.130
0.127
0.140
0.176
0.131
0.115
0.158
0.088
0.087
0.038
0.533
0.549
0.552
0.558
0.553
0.535
0.546
0.564
0.585
0.600
0.532
0.544
0.546
0.518
0.549
0.536
0.538
0.540
0.552
0.547
0.553
0.573
0.564
0.556
0.545
0.535
0.543
0.576
0.555
0.541
0.456 (0.023)*
0.482 (0.023)*
0.497 (0.023)
0.482 (0.024)*
0.481 (0.022)*
0.500 (0.023)*
0.485 (0.024)*
0.458 (0.023)*
0.474 (0.023)
0.474 (0.025)*
0.457 (0.023)*
0.525 (0.024)
0.507 (0.023)*
0.520 (0.023)
0.473 (0.023)
0.486 (0.023)
0.502 (0.023)
0.483 (0.023)*
0.512 (0.023)
0.426 (0.026)*
0.504 (0.023)
0.463 (0.025)*
0.521 (0.023)
0.558 (0.023)
0.476 (0.023)*
0.453 (0.023)*
0.495 (0.023)
0.498 (0.026)
0.489 (0.023)
0.471 (0.023)*
2

east west west
Thuringian
Forest west west west west east
Mean
T17.1
T17.2
T18.1
T19.1
T19.2
T19.3
T20.1
T20.2
T20.3
T21.1
T21.2
T22.1
T23.2
T24.1 mean
Goldene Lauter
Lange Lauter tributary to Lubenbach
Haselbach
Kohlenbach
Kienbach tributary to Wilde Gera
Zahme Gera
Steinbach
Kleiner Schmiebach
Kleiner Grundbach
Ebersbach
Inselwasser
Emse
10.76375
10.78458
10.70077
10.68485
10.66157
10.62840
10.74571
10.80120
10.74717
10.52383
10.54236
10.49370
10.46414
10.41510
50.64857 24
50.62752 25
50.68788 24
50.69888 24
50.70497 26
50.71829 22
50.69713 20
50.65852 21
50.66753 24
50.77255 24
50.77072 24
50.82282 24
50.84589 24
50.85542 24
0.104
0.080
0.032
0.092
0.011
0.038
0.056
0.091
0.038
0.147
0.073
0.110
0.014
0.088
3.988
3.417
3.335
3.192
3.179
3.349
3.543
3.553
3.309
3.268
3.510
3.387
3.266
3.204
3.235
3.339
0.551
0.507
0.511
0.486
0.499
0.508
0.534
0.533
0.494
0.500
0.519
0.531
0.481
0.468
0.516
0.506
0.454 (0.023)*
0.47 (0.023)
0.47 (0.023)
0.453 (0.023)*
0.503 (0.022)
0.514 (0.024)
0.503 (0.025)
0.449 (0.025)
0.481 (0.023)
0.443 (0.023)*
0.492 (0.023)*
0.428 (0.023)
0.461 (0.023)
0.471 (0.023)*
3

Supplementary Table 2: Characteristics of 22 microsatellite loci for Drusus discolor . For each locus we provide
GenBank accession numbers for the clone sequence, range size of alleles, number of alleles in total (and in
BF/TF), and observed and expected heterozygosity. primer sequence, repeat motif, annealing temperature and
MgCl
2
concentration for PCR.
Locus
Accession no. range number of alleles
Ho/ He Primer sequence 5'-> 3' Motif
T
A
[°C]
MgCL
2
[mM]
Dd02 1)M3
Dd03 1)M3
Dd04 1)+M4
Dd09 1)M2
Dd11 1)M5
Dd12 1)M2
Dd17 1)M3
Dd27 2)M2
Dd31 2)M5
Dd33 2)M3
Dd49 2)
Dd52 2)M6
Dd54 2)M4
Dd59 2)M1
Dd61 2)M1
Dd66 2)+M2
Dd67
DQ838653.1 98-224
DQ838654.1
DQ838659.1
DQ838655.1
DQ838656.1
DQ838657.1
DQ838658.1
JF437616.1
JF437617.1
JF437620.1
JF437619.1
JF437620.1
JF437621.1
JF437623.1
JF437624.1
JF437625.1 to be provided
176-216
247-256
233-263
194-214
197-307
181-195
182-226
211-227
236-278
198-312
138-174
142-150
203-211
222-239
121-149
10 (8/8)
11 (8/11)
94-310 41 (40/30)
4 (4/2)
11 (9/9)
7 (6/5)
25 (25/11)
5 (5/3)
12 (12/10)
8 (8/5)
7 (6/5)
34 (24/21)
18 (18/6)
5 (5/3)
5 (5/4)
10 (8/6)
9 (7/7)
0.420/
0.569*
0.608/
0.596
0.791/
0.807
0.639/
0.766*
0.317/
0.395*
0.578/
0.600
0.331/
0.431
0.194/
0.373*
0.403/
0.414
0.736/
0.904
0.271/
0.278
0.366/
0.615*
0.378/
0.373
0.755/
0.788
0.438/
0.428
0.720/
0.712
0.493/
0.659*
L: CAAGAAAGAAAGCTTCCGAATG
R: GACCACCTACAGATATACGTAAAGGA
L: TGGAACAATCGAAGTTATTTAGGA
R: GCTGCTCAAGATGAACACCA
L: GTTTCTTCGGCTCATTTCCA
R: AACACGCAGACCGGTAAAAC
L: GTGTCTTGGTCGTAAGGCAGCCATAAA
R: AATCAGGAGGTTGCGGTTC
L: CGGGGAAATACGCAGTTTTA
R: GTGTCTTACTTAAATCGGGCCATGTGA
L: GCGCCAAATGTCAGATGTAA
R: CGCTGTCTGGATCAGACGTA
L: ATCGGAAAAATGGTCAAGCA
R: GTGTCTTCGATCGGACTAGGAGGAAAC
F: TTTGATAGATACCGCACTTAGGG
R: CCTGGAATCCACTGCCTAAA
F: TTCCGGAACGAATAATGCTC
R: GAATGGAGACCACTCGGATG
F: GCAAGAAGTGTGTGCCAAGA
R: GGCCCTAATCTTCGATTTGA
F: TGACCTTGCTTTGACTTGAATG
R: TCAAAATGCGTGGCTGATTA
F: AATGGATTCTTTGCATTTGG
R: CGGCAACATACCACATAACA
F: CTGCCCCATGTGGAACTTAT
R: CAATAGGTCGGATGGAGAACA
F: TCTCGCATTTCGTTGTTATCTG
R: ACACATTGTGCCATCGGTAA
F: ATGCTCGTTGACGATGTTTG
R: TCCAAAAATTACGACGTTTGC
F: AGTCGACACCCCATCGTAAA
R: CTGCCATATCCACGAGCTACT
F: TGCAGCAACAAATTCTCCAA
R: GTGGTGTTCTGTGGCGAAG
F: ATGATTGGCCGACTTTGTTGC
Dd68 M7
Dd70 M7
Dd72 M7 to be provided to be provided to be provided
189-197
250-276
174-189
5 (5/3)
7 (7/3)
6 (5/5)
0.452/
0.434
0.381/
0.423
0.617/
0.624
R: TCTGCGCTCCGGATACTCTTG
F: ATGAGGAGATCTCGGCTTTTG
R: ATTACGCCATCTACCTTCCTTC
F: CGATTCGCCAACTTCAGTCC
R: ATCCGGTGTCAATGTATGCC
F: AGTGGGTCTATGGCATTTCC
R: TGAACAACCACGGTGACATT
Dd75 M7 to be provided
154-166 5 (4/3)
0.290/
0.306
0.506/
0.542
F: TACCCGGCATACTTAGCAATG
Dd77 M6 to be provided
110-184 18 (15/11)
R: AAAATCGGTGTTTTGTTCACG
+ Locus was excluded from all analysis due to impact of null alleles
* Locus was excluded from Structure analysis due to a deviation from HWE
M1 to M7 number of PCR multiplex
1) published in Pauls et al.
(2007)
2) published in Geismar et al.
(2011)
(CATCTAT)17 58
GTTA)9
(GTTGTTT)19 56
(TGT)8
(TAAC)7
(CATA)5
(GTAT)7
(AT)8
(GATT)6
(GTTA)6
(TAAA)5
(GTTT)5
(TA)9
(AT)7
(AT)9
(TTTTA)6
(CA)14
(GT)7
(AT)6
(ACA)7
(AAT)7
(AT)11
58
58
56
58
58
58
55
58
58
58
52
58
58
60
60
2.5
2.5
2.5
3
2.5
3
2.5
2.5
2.5
2.5
2
2.5
2.5
2.5
2.5
3
2.5
58/60 2.5
58/61 2.5
58/62 2.5
58/63 2.5
58/64 2.5
4

Supplementary Table 3: Genetic differentiation between the samples from (a) Black Forest and (b) Thuringian Forest: lower diagonal F
ST
and D Jost (upper diagonal). * indicates significant values (p < 0.05) and bold values are significant after Bonferroni correction. Negative F
ST
and D Jost values were given as 0.
a FB01.
1
-
FB02.
1
0.04*
FB02.
2
0.05
FB02.
3
0.02
FB03.
1
0.02
FB03.
2
0.02
FB03.
3
0.01
FB03.
4
0.00
FB04.
1
0.01
FB04.
2
0.00
FB05.
1
0.02
FB05.
2
0.00
FB05.
3
0.01
FB06.
1
0.03
SL07.
1
0.03
SL07.
2
0.02
SL07.
3
0.01
SL08.
1
0
SL08.
2
0.02
SL09.
1
0.02
SL10.
1
0.02
RB11.
1
0.09
RB12.
1
0.03
RB12.
2
0.08
RB12.
3
0.11
RB13.
1
0.07
RB13.
2
0.08
S14.
1
0.11
S15.
1
0.12
S16.
1
0.10
RB11.
1
RB12.
1
RB12.
2
RB12.
3
RB13.
1
RB13.
2
SL07.
1
SL07.
2
SL07.
3
SL08.
1
SL08.
2
SL09.
1
SL10.
1
3
FB03.
4
FB04.
1
FB04.
2
FB05.
1
FB05.
2
FB05.
3
FB06.
1
FB01.
1
FB02.
1
FB02.
2
FB02.
3
FB03.
1
FB03.
2
FB03.
0.02
0.03
0.01
0.01
0.00
0.01
0.00
0
0
0.01
0.00
0.01
0.01*
0.01*
0.00
0.00
0
0.01
0.01
0.00
0.06
0.03
0.04
0.06
0.04
0.05
-
0.02*
0.02*
0.02*
0.01
0.02*
0.02*
0.01*
0.01
0.02*
0.02
0.02
0.02
0.02*
0.02
0.01
0.01*
0.01*
0.02*
0.02
0.07
0.04
0.04
0.06
0.06
0.07
0.02
-
0.03*
0.04
0.02*
0.03
0.03
0.02
0.03
0.03
0.02
0.02
0.03
0.04
0.04
0.01
0.02*
0.03
0.03
0.03
0.06
0.03
0.03
0.05
0.06
0.07
0.04*
0.05
-
0.02*
0.02*
0.02*
0.01
0.01
0.00
0.01
0.01
0.01
0.01*
0.03
0.02*
0.01
0.00
0.01*
0.01
0.02*
0.07
0.03
0.06
0.07
0.06
0.06
0.03
0.06
0.04
-
0.00
0.01
0.01
0.01
0.00
0.01
0.01
0.02*
0.01
0.02*
0.01
0.02*
0.01
0.00
0.01
0.01*
0.08
0.04
0.05
0.07
0.07
0.07
0.01
0.03
0.04*
0.00
-
0.00
0.00
0
0.00
0
0.00
0.01
0.00
0.01
0.00
0.01
0.01
0
0.01
0.00
0.05
0.03
0.02*
0.04
0.05
0.05
0.03
0.05
0.03
0.01
0
-
0.01
0.01
0
0.01
0.00
0.01
0.01
0.01
0
0.01
0.00
0.00
0.01
0.01
0.07
0.03
0.04
0.05
0.06
0.06
0.03
0.05*
0.01
0.01
0.00
0
-
0
0
0
0.00
0.01
0.01
0.01
0.00
0.00
0.00
0
0.01
0.00
0.06
0.03
0.04
0.05
0.05
0.05
0.04*
0.04*
0.01
0.01
0.00
0.01
0
-
0
0
0
0.00
0.00
0.01
0.00
0.00
0.00
0
0.00
0.00
0.07
0.03
0.04
0.05
0.06
0.06
0.02
0.05
0
0.01
0.00
0
0
0
-
0
0.00
0.00
0.00
0.00
0
0.00
0
0
0
0
0.08
0.03
0.05
0.07
0.06
0.06
0.04*
0.06
0.02
0.01
0.00
0.01
0
0
0
-
0.00
0.01
0.00
0.01
0.00
0.01
0.00
0.00
0.01
0.00
0.08
0.04
0.04
0.07
0.07
0.07
0.04*
0.04*
0.02
0.01
0
0
0.00
0
0
0
-
0.01
0.01
0.02*
0.00
0.00
0.00
0.00
0.01
0.01
0.07
0.03
0.04
0.06
0.05
0.06
0.04*
0.04*
0.01
0.05*
0.02
0.02
0.01
0.01
0.00
0.02
0.02
-
0.01*
0.02
0.01
0.01
0.00
0.01*
0.01
0.01
0.08
0.03
0.04
0.05
0.07
0.06
0.04
0.06
0.04
0.02
0
0
0.01
0.02
0.01
0.01
0.01
0.04*
-
0.01
0.01
0.02*
0.01
0.01*
0.01
0.01*
0.09
0.05
0.05
0.07
0.08
0.08
0.05
0.07
0.05
0.03
0.01
0.00
0.02
0.01
0
0.00
0.03
0.04*
0.02
-
0.00
0.02*
0.01*
0.00
0.01
0.01
0.08
0.04
0.04
0.07
0.07
0.07
0.06
0.07
0.04*
0.02
0.01
0
0.01
0.01
0.00
0.00
0.01
0.02
0.01
0.00
-
0.01*
0.00
0.00
0.01
0.00
0.07
0.03
0.04
0.06
0.05
0.05
0.03
0.02
0.02
0.04*
0.02
0.01
0.01
0.01
0.01
0.02
0.01
0.01
0.05*
0.02
0.03
-
0.00
0.01
0.02*
0.01*
0.06
0.02*
0.02
0.05
0.04
0.05
0.03
0.03
0.00
0.02
0.03
0.02
0.01
0.01
0
0.01
0.01
0.01
0.03
0.03
0.02
0.02
-
0.00
0.01
0.01
0.07
0.03
0.04
0.06
0.06
0.06
0.03
0.06
0.02
0.01
0
0.01
0
0
0
0.00
0.00
0.02
0.02
0.00
0.02
0.02
0.02
-
0
0
0.07
0.03
0.04
0.06
0.06
0.06
0.04
0.06
0.02
0.02
0.00
0.02
0.01
0.00
0
0.01
0.01
0.02
0.02
0.02
0.02
0.04
0.03
0
-
0
0.08
0.04
0.05
0.07
0.07
0.07
0.04*
0.06
0.03
0.02
0.00
0.01
0.01
0.01
0
0.01
0.01
0.02
0.02
0.01
0.01
0.03
0.03
0
0
-
0.08
0.04
0.05
0.07
0.07
0.07
0.11
0.10
0.09
0.11
0.08
0.09
0.08
0.10
0.09
0.10
0.09
0.09
0.12
0.11
0.10
0.08
0.09
0.10
0.10
0.11
-
0.01
0.02*
0.05
0.02*
0.02*
0.07
0.06
0.06
0.07
0.04
0.05
0.04
0.04*
0.05
0.06
0.04*
0.04*
0.08
0.07
0.05*
0.03
0.05
0.06
0.06
0.07
0.02
-
0.01
0.03
0.02*
0.02*
0.07
0.07
0.11
0.09
0.04*
0.07
0.06
0.08
0.09
0.08
0.07
0.06
0.09
0.08
0.07
0.04*
0.09
0.07
0.09
0.09
0.05*
0.02
-
0.02
0.02*
0.03
0.10
0.08
0.13
0.13
0.08
0.10
0.09
0.10
0.12
0.13
0.11
0.09
0.12
0.12
0.11
0.09
0.10
0.10
0.10
0.11
0.08
0.05*
0.04
-
0.04
0.04
0.11
0.10
0.10
0.12
0.10
0.09
0.08
0.10
0.10
0.11
0.10
0.09
0.13
0.11
0.08
0.06
0.10
0.10
0.10
0.12
0.03
0.03
0.04
0.07
-
0
0.11
0.11
0.10
0.12
0.10
0.09
0.09
0.11
0.10
0.12
0.12
0.10
0.13
0.12
0.08
0.09
0.10
0.11
0.11
0.12
0.04
0.03
0.06
0.07
0
-
0.15
0.13
0.14
0.14
0.11
0.13
0.11
0.12
0.13
0.14
0.11
0.12
0.14
0.14
0.12
0.12
0.14
0.12
0.12
0.12
0.13
0.06
0.10
0.11
0.11
0.11
0.13
0.12
0.13
0.09
0.11
0.10
0.12
0.10
0.12
0.10
0.09
0.15
0.11
0.11
0.11
0.11
0.11
0.11
0.13
0.14
0.16
0.12
0.13
0.19
0.17
0.17
0.08
0.05
0.11
0.10
0.09
0.12
0.11
0.10
0.11
0.11
0.09
0.11
0.10
0.13
0.11
0.10
0.06
0.11
0.11
0.12
0.13
0.12
0.13
0.15
0.16
0.16
0.06 0.09 0.07 0.08 0.08 0.06 0.08 0.06 0.06 0.07 0.07 0.05 0.06 0.07 0.08 0.06 0.07 0.07 0.06 0.07 0.07 0.08 0.04 0.05 0.06 0.06 0.06 - 0.19 0.17
S14.1
0.07 0.07 0.06 0.07 0.06 0.06 0.06 0.06 0.06 0.06 0.07 0.05 0.08 0.07 0.07 0.07 0.07 0.06 0.06 0.07 0.08 0.09 0.07 0.07 0.10 0.10 0.10 0.09 - 0.14
S15.1
0.08 0.06 0.04 0.08 0.08 0.06 0.08 0.07 0.07 0.07 0.08 0.07 0.08 0.08 0.09 0.08 0.07 0.06 0.08 0.08 0.08 0.10 0.09 0.08 0.10 0.11 0.12 0.11 0.07 -
S16.1
b
T17.1
T17.1
-
T17.2
0.02*
T18.1
0.04
T19.1
0.01
T19.2
0.01
T19.3
0.01
T20.1
0
T20.2
0.03
T20.3
0.01
T21.1
0.07
T21.2
0.03*
T22.1
0.05
T23.1
0.07
T24.1
0.11
T17.2
0.01 - 0.07 0.01 0.01 0.00 0.01 0.02 0.02 0.05 0.01 0.03* 0.04* 0.05
T18.1
0.05 0.06 - 0.07 0.06 0.07 0.02 0.06 0.08 0.11 0.10 0.08 0.13 0.14
5

T19.1
T19.2
T19.3
T20.1
T20.2
T20.3
T21.1
T21.2
T22.1
T23.1
T24.1
0.01
0.02
0.02*
0
0.03
0.02*
0.04
0.03
0.05
0.07
0.10
0.03
0.02*
0.01
0.02
0.02*
0.02*
0.04
0.01
0.02*
0.04
0.05
0.08
0.07
0.09
0.08
0.08
0.07
0.06
0.04
0.06
0.12
0.12
-
0.03
0.03
0.03
0.03
0.02
0.02*
0.01
0.03
0.05
0.08
0.01
-
0.00
0.01*
0.02*
0.01*
0.05
0.02*
0.03
0.04
0.07
0.01
0
-
0.02*
0.01
0.02*
0.04
0.01
0.02*
0.04
0.05
0.01
0
0.01
-
0.02*
0.02*
0.03*
0.02*
0.04
0.06
0.08
0.03
0.05
0.06
0.03
0.03
0.02
0.01
0.02
-
0.07
0.08
0.01
-
0.06
0.03
0.04
0.01
0.02
0.01
0.04
0.05
0.09
0.05
0.07
-
0.05
0.05
0.07
0.05
0.06
0.09
0.06
0.04
0.03*
0.03
0.03
-
0.05
0.02
0.01
0.02
0.05
0.04
0.04
0.03*
0.05
0.06
0.07
-
0.04
0.03
0.04
0.04*
0.04
0.08
0.05
0.06
0.07
0.06
0.05
0.05
0.04
0.06
0.08
-
0.07
0.09
0.10
0.05
0.05
0.10
0.07
0.08
0.06
0.09
0.10
-
6

1
2
3
4
5
6
7
8
9
Supplementary Table 4: Averaged estimated relative contributions of environmental and bioclimatic variables of the SDM. Contribution to the model of each variable is indices and importance calculated on permutated test and background data.
Variable bioclimatic/environmental description
Percent contribution
Permutation importance bio1 bio4 slope bio9 forest bio2 bio7 scrub_herbage ind_urban wetlands waterbodies annual mean temperature temperature seasonality (SD*100) slope mean temperature of driest quarter forest mean diurnal range temperature annual range scrubs urban area wetlands waterbodies
52.1
21.2
10.8
8.5
2.6
2.5
2.1
0.2
0
0
0
41.2
17.4
8.5
24.2
0
5.8
2.7
0
0.1
0
0
Supplemantay Table 5: Correlation coefficient r of correlation of modified distances against Euclidian distance of BF and TF, highly correlated comparisons (<0.8) are indicated by an asterisk.
Modified distances
Complete
Black Forest
Within catchments
Among catchments
Thuringian Forest
Complete
Within catchments
Among catchments
Net suitability distance
Normalized gross suitability distance
Relative least-cost distance
Normalized absolute least-cost
Relative least-cost
Stepping stone distance
0.74
0.96*
0.47
0.92*
0.60
0.93*
0.82*
0.76
-0.02
0.74
0.05
0.61
0.73
0.96*
0.46
0.91*
0.59
0.92*
0.96*
0.99*
0.49
0.89*
0.46
0.94*
0.92*
0.91*
0.38
0.76
0.37
0.83*
0.96*
0.99*
0.45
0.87*
0.42
0.93*
7

10
11
12
13
14
Supplemantay Table 6: Results of multimodal inference model selection approach. Models are ranked by AIC, from smallest to largest.; only those models with ΔAIC < 2 are shown here. Distance variables are abbreviated as followed: Euclidian distance (ED), Net suitability distance (INS), Normalized gross suitability distance (IGSn),
Normalized absolute least-cost (aLCn), Relative least-cost (rLC), Stepping stone distance (SSD)
Category Model log-likelihood AIC
ΔAIC
Model weight
BF complete
BF among catchments
BF wihin catchments
TF complete
TF among catchments
TF wihin catchments
ED+IGSn
ED+IGSn+rLC
ED+IGSn+INS
ED+IGSn
ED+IGSn+INS
ED+IGSn+rLC aLCn+rLC aLCn+SSd+rLC aLCn+rLCd+rLC aLCn+IGSn+rLC aLCn+ED+rLC
ED+rLC
ED+IGSn
ED
ED+IGSn+rLC
ED+IGSn
ED+rLC
ED
ED+Igsn+rLC
NA
ED+SSD
SSD
1124.709 -2241.419
1124.710 -2239.420
1124.710 -2239.419
1063.017 -2118.034
1063.029 -2116.058
1063.020 -2116.041
75.499 -142.998
75.785 -141.570
75.741 -141.482
75.508 -141.017
75.500 -141.000
212.547 -417.094
212.530 -417.059
210.769 -415.538
212.553 -415.107
191.296 -374.592
191.292 -374.583
189.481 -372.962
191.308 -372.616
25.713 -47.427
27.462 -46.923
26.300 -46.599
0.534
0.197
0.197
0.532
0.198
0.196
0.155
0.076
0.073
0.058
0.057
0.355
0.349
0.163
0.132
0.355
0.353
0.157
0.132
0.356
0.277
0.235
0.000
1.999
1.999
0.000
1.976
1.994
0.000
1.428
1.515
1.981
1.998
0.000
0.035
1.556
1.987
0.000
0.009
1.630
1.976
0.000
0.504
0.828
ED 25.716 -45.433 1.994 0.131
8