Tender Specifications
Chemical Glass Wares & Equipment’s for IBMS & KMU
Section – III
S.No
Specification / Bid Form
Description
Specifications
1
Tanks for liquid
Gases with
Accessories
Liquid Nitrogen NO2, Oxygen and CO2 Gas, 20 liters capacity with
regulators where required. With six stainless-steel holders to
accommodate vials. Canisters are numbered and color-coded to simplify
sample identification. Durable aluminum construction and vacuum
insulation. Narrow-mouth design minimizes LN2 evaporation. Secure
locking clasp
2
Water Distillation
Unit
Auto start, Capacity: 4-6litre/hour. Power: 220VAC. 50/60Hz. capable of
producing pyrogen free distillate.
b) Distillate temperature is around 20-50 degree C
Conductivity: 2.0 to 4.0 µS/cm
Distilled Water Quality: Pyrogen Free
pH: 5.5-6.0
Hygrometer, adjustable humidity, water tank safety setup, taking the
water tank without working automatically cutting off power without
water, lower power 360 degree auto turning. Humidification Capacity 1 to
10 L/h, Voltage/Cycle 220VAC/50HZ , Work Temperature -5 to 40, Air
Volume 350m³/h, Setting range:25-80% RH Control Humidity/Time
4
Humidifier
5
Thermobrite Stat
Spin Slide Hybridizer
Capacity 10 -14 Slides,
Processing Time: Programmable 0 to 100 hours
Continuous mode
Power 220VAC/50HZ
Temperature Control Programmable 20-99° C
Ambient Operating Temperature 5-40° C(41-104° F)
Ambient Operating Humidity 10-80% relative
European, USA, Japan made
6
Real Time PCR with
HRM software
Real Time PCR with HRM software
Complete system having capability of 4 colors multiplexing for the
detection of FAM, SYBR, HEX, ROX, JOE, VIC and CY5. Block capacity 96 x
0.2 ml pcr tubes or 96 well PCR plate or 12 x 8 well strips. System should
have following specifications.
Dye Selection:
Excitation and emission
Reaction Volume:
10μl to 30μl
Chemistries Supported:
Thermal System:
SYBR, Probe, HRM
Six peltiers made from two ceramic
plates with semi-conductor
Unit
price
including
all taxes
7
Refrigerated Shaking
Incubators
8
Pressure Cooker
Scientific
elements, 96 well
Thermal System Temperature Range:25.0 – 99.9°C Heating: 6°C/sec
Cooling: 2.5°C/sec Accuracy: ± 0.2°C
Dynamic Range:
9 orders of magnitude
Comparative Quantitation: 2x (Range 1.3x to 3x), 10x (Range 6.5x to 15x)
Multiplex:
4 channels
Sensitivity (FAM):
Discriminates between 2 fold
populations ranging from 100k to
12 copies with 95% confidence
Cq uniformity:
Cq St Dev <0.20 at fast cycling (5sec
95°C/10sec 60°C)
Uniformity:
± 0.4°C
Tm Uniformity with HRM: ± 0.1°C St. Dev. Less than or equal to 0.03°C
Data acquisition time:
3 sec for data acquisition in all six
channels
Electrical Power (input):
100 – 240VAC, 50/60Hz, 1100A
Operating Environment:
18 – 35°C (65 – 95°F), 85% noncondensing humidity 7500 feet, max
altitude
Operating System:
Windows 7
Run Modes:
• Stand alone
• PC connected
• LAN connected: more than 20
instruments can be connected and
monitored remotely
• Two USB ports for extending to
external devices
European, USA, Japan made
Forced Convection Uniform Temperature Control,
Electronic Control with Safety Back-Up,
Optional Humidifier, Optional Communication interface,
Temp. Range: -5oC to 70oC,
Controller: Microprocessor PID Multi-Function controller,
Display: LED 4 Digits Display,
Material Inner: Stainless Steel (SUS 304),
Circulation: Forced Convection Air Circulation, Safety: over Temp. CutOff/ over current breaker flask capacity: 10mL to 2.8L,
Shaking speed range: 50 to 400rpm Shaking orbit: 0.75 in. dia. (19mm)
Digital keypad control and LED display for speed, run time, alarms and
temperature,
Timer: 0.1 to 99.9 hours, Capacity: 60 to 70 liters
European, USA, Japan made
Stainless steel, pressure setting between 0.6 - 1 bar (10 - 15 psi)
Capacity; 10-15 liter
9
Glass Door
Refrigerator
Double door (glass), upright conventional local make, 900 L volume, 28°C. Suitable for clinical specimens and chemicals (Pakistan made).
10
-20 Freezer
11
Manual
Tissue
Arrayer MTA-1
Single door with inner glass doors and compartment, upright, 350-400 L
volume, having Display for temp and locked door safety,
Suitable for clinical specimens and chemicals
Speed (cores/hour): 30-70,
Default block capacity: 1
Max block capacity: 4 Note! with indexer RBH1 (optional), Donor block
height: 3 cm
12
Digital dry block
heating system for
micro tubes
13
UPS
14
Online
system
15
Horizontal laminar
flow clean benches
Width 42"
40"
Depth 36"
20"
Height 38"
28"
68"
Air Velocity
60-100LFM
Noise Level
<60DBA
Light
1 x 20W
Filtration
HEPA 99.95%
Controls Microprocessor
Electric Supply: 220V, 50/60Hz
Slide type door, Internal Work Area Space: 3-6 Sq. ft,
Double Blower System.
16
Refrigerated
Centrifuge
(Multipurpose, with
Swinging buckets)
MTP rotor, for 12 x 15ml tubes, 60ml Falcon tube rotor and Micro test
tube, speed range 200 to 20,000 rpm, Speed Set/Display, Timer
Stabilizer
Temperature Range (Metric) Ambient +5° to +130°C
Temperature Uniformity ±0.2° at 37°C Temperature Resolution ±0.3° at
37°C Height (English) 2-4 in.
Height (Metric) 8-10cm Length (English) 10-15 in.
Length (Metric) 35-40cm Width (English) 8-10 in.
Width (Metric) 20-25cm Wattage 150w Hertz 50/60Hz Amperage 1.25A
Certifications/Compliance CE marked, CSA approved Dimensions (L x W x
H) (35-40 x 20-25 x 8-10cm) 3 KVA dry rechargeable batteries with standard backup time, automatic
charging, low noise beep, inbuilt relay system for power fluctuation.
20 KVA online stabilizer with servo motors, can regulate the voltage
between 220-230
Set/Display 00 sec–99 min+ continuous, Temperature Set/Display Angle
+ Swinging Rotor with Automatic rotor recognition
Stores up to 50 routine programs
Menu guided operation in English “at set rpm” for max. reproducibility
Automatic lid opening following application sequence (only 5430)
Temp. range: -11 °C - +40 °C
+ 4 °C at max. speed with all rotors
‘Continues cooling’ after the centrifugation process
‘Fast Temp’ for achieving required temperature in shortest possible time,
having separate rotors, buckets, and Adaptors.
European, USA, Japan made
The Minifuge complete with two rotors, one for micro centrifuge tubes
(24 x 1.5 mL tubes) and another for 0.2 mL PCR strip tubes. Max RPM
16,000
17
Mini-Fuge (Microcentrifuges)
18
Digital Orbital Shaker Variable shaking speed: 50 to 450rpm
Holds up to 8.0 lbs
Automatic load balancing system
Continuous or timed operation with automatic switch-off,
Timer 0—99 min or continuous,
Motion/orbit size Circular, 2-5 mm,
capacity: 60 x 1./5.0 mL micro tubes,
microplates, microplates Ambient operating range +4° to 70°C,
Electrical 230V~, 50 Hz or 120V~, 60 Hz
Maximum speed for 115V unit 1,400rpm
19
Nano Drop UV
Spectrophotometer
Wide spectral range (190-840nm) for measuring a variety of sample e.g
Peptides, DNA and RNA, Purified protein, Toxicology assays, Gold
nanoparticles, Colorimetric protein assays, No dilutions required, even for
highly concentrated samples Patented sample retention system
automatically optimizes path length to accommodate low and high
concentrations 1 to 20,000 ng/µL ds DNA), required sample size 0.5 – 1
µL, sample purity ratios (260/280 nm and 260/230 nm measurement
time 3 -15 seconds, friendly software. With computer and other all
accessories. Lower Limit of Detection 4 ng/µl (dsDNA) 0.12 mg/ml (BSA).
Maximum Concentration 1,500 ng/µl dsDNA) 45 mg/ml (BSA).
Measurement Time < 5 seconds. Footprint 16 x 11.5 cm. Weight 0.8 kg.
Sample Pedestal Material of Construction 303 stainless steel and quartz
fiber. Operating Voltage 6 V (DC). Operating Power Consumption 18 W.
Standby Power Consumption ≤ 2.5 W. Software Compatibility Local
control only
European, USA, Japan made
20
Electronic Balance
Capacity 10mg to 500g, readability up to 0.001g, communication with
printers, • Bright LCD display - easy to read in all lighting conditions
including bright sunlight, Reads in multiple units - allows flexibility of
weighing across many disciplines, Adjust balance response to differing
environmental conditions for more controlled results
European, USA, Japan made
Capacity 30 L, Convection, Power Consumption (Microwave)10001800W, LED Display, Power Source 230v / 50hz, Digital timer control,
Programmable heating facilities
21
Microwave Oven
22
Heating, Ventilation
& air conditioning
system
Should be design according to Lab infrastructure.
23
-86 C freezer
Includes Voltage Booster, CFC Free: Foam Insulation 127 Mm(5 Inches),
Access Port, Heavy-Gauge Steel Cabinet, Heavy-Duty Swivel Casters, ClawLike Inner Door Latches, Key Lock, Two (2) Air Insulated Inner Doors,
Four(4) Shelves-Three (3) Adjustable, Multi-Point Gasket Seals, Capacity;
500-550 liter, European, USA, Japan made
24
Digital LCD Inverted
Biological
Microscope
Complete with objectives and LED illumination system having:
Optical System: Infinity corrected system, 45 mm parafocality distance.
Field of view 22 mm.
Head: Trinocular: 30° inclined, 360° rotating.
Interpupillary distance: 48 - 75 mm.
Adjustable dioptric compensation.
Eyepieces: Wide field EWF10x/22mm, eyeglass-compatible
Nosepiece: 5 positions, with bidirectional rotation on ball bearings and
click stop.
Objectives: Long working distance (LWD) infinity corrected (IOS) plan
achromatic:
4x/0.10 (working distance 18 mm),
phase contrast 10x/0.25 (working distance 10 mm),
phase contrast 20x/0.40 (working distance 5.1 mm),
40x/0.60 (working distance 2.6 mm),
Corrected for 1.2 mm cover glass.
Specimen stage: Size: 250 x 230 mm.
Translator with lowered ergonomic coaxial controls.
X-Y translation: 119 x 70 mm. Interchangeable metallic inserts for
specimen slides, Petri dishes and flasks.
Focusing system: Macro and micrometric regulation, with coaxial knobs
on both sides of the stand. Adjustable tension.
Condenser: Long working distance condenser, numerical aperture 0.30,
working distance 72 mm. The condenser can be removed in order to
increase the working distance to 150 mm.
Illumination system: X-LED8™ system, with adjustable intensity, filter and
phase ring holder and aperture diaphragm. European, USA, Japan made
25
PT Link,PreTreatmeent Module
for Tissue Specimens
DAKO Company
B. Laboratory Chemicals and Consumables
No
Item Name
Specifications
Unit Price (Inclusive
of all Taxes)
1
DNA ladder 50,100,500,1000
Maximum resolution 1.5% Agarose gel
2
PCR master mix
Ready Reaction for Routine PCR
3
Genomic DNA extraction kit
Human whole blood, tissue, Microbial
cells
4
RNA extraction kit
Human whole blood, Plant, Microbial
cells
5
EDTA
Molecular Biology grade
6
dNTPs or dATP, dCPT, dGTP, dTTP
Molecular Biology grade
7
Taq Polymerase
Ready for Routine PCR
8
10ml Syringe fitting water filters
Minimal pore size for microbes escape
9
Collagenase enzyme
Ready to use
10
Boric acid
Molecular Biology grade
11
C-tab Enzymes
Ready to use
12
Tris Base
Molecular Biology grade
13
Racks
For Eppendorf tube, Test tubes etc.
14
Ultra-pure Tris
Molecular Biology grade
15
c DNA Synthesis Kits
For Molecular use
16
Viral DNA Extraction Kits
For PCR
17
Reagents for routine chemistry (Roche)
for COBAS CIII
Glucose, ALT, Bilirubin (Total)
Bilirubin (Direct) Gamma GT, urea,
creatinine, Cholesterol, Triglyceride,
HDL, LDL, controls and calibrators
18
Reagents for Sysmax Xs 500i
Diluent, Lyse, calibrators
19
Protein standards markers
20
Bradford assay reagents
21
Oligo (dt) primers
22
Eva Green qPCR master mix
For high resolution melt curve analysis
on ABI 7500 Real time thermal cycler
23
Viral DNA Extraction Kits
For PCR
24
Silver Nitrate
25
Chloro auric acid
26
Palladium chloride
27
Platinum tera chloride
28
Developer for SDS-PAGE
29
Fixer for SDS-PAGE
30
Acridine Orange Stain
31
Antibodies
(Her2/Neu,
Estrogen
receptor, Progesteron Receptor, Ki-67,
P53)
Slide Racks with containers for antigen
retrieval
Endogenous Peroxidase Block
32
33
36
Secondary antibody both anti-mouse and
anti-rabbit
3,3’-Diaminobenzidine
tetra
hydrochloride
Gills haematoxylin
37
Haematoxylin Z
38
Faramount Mounting Medium, Aqueous
39
Coplin Jars
40
Slide Racks for Immunohistochemistry
41
Containers for slide Racks
Immunohistochemistry
Staining Troughs and Racks
34
35
42
43
44
45
46
47
(Dako EnVision)
(Dako EnVision)
(DAKO)
for
PT Link, Pre-Treatment Module for
Tissue Specimens
Tris EDTA
Qiagen QIAmp DNA micro Ready to use
kit,200 rxn
Qiagen DNeasy blood and tissue Ready to use
kit, 250 rxn
Ready to use
Qiagen RNeasy mini kit, 250 rxn
48
High capacity cDNA
transcription kit, 250 rxn
49
Cell culture bottles
reverse Ready to use
Sterilized, Filtered, DNA/RNAs free,
For cell culture, Volume 60ml