March 8, 2013 – Chapter 14 Questions 1) a) 6 domestic cats b) two c) one 2) Outline two uses of restriction enzymes in biotechnology. Genetic modification and DNA profiling. 3) Suggest why therapeutic cloning might be opposed by advocates of women’s rights. Advocates of women’s rights may feel that therapeutic cloning turns 4) Compare the polymerase chain reaction with DNA replication as it occurs in cells. 5) List three examples of where cloning occurs naturally. Asexual reproduction, identical twins, 6) a) IV – it travelled the farthest from the origin. Because the gel opposes movement, the smallest fragments are the ones that are able to slip through and get the farthest away from the origin. b) Child 2 7) A March 8, 2013 – DBQ on p. 171 1) State the number of codons in the genetic code. 64 codons. 2) Determine the fraction of codons that are stop codons in the genetic code. 3/64 3) In Table 2, the codons could start with the first, second or third base. These correspond to three different reading frames (RF1, RF2 or RF3). Determine which of the reading frames, 1, 2, or 3 above, might be an open reading frame. RF3. March 8, 2013 – DBQ on p. 171 1) Deduce the diploid chromosome number of mice. 42 chromosomes. 2) Identify the two human chromosome types that are most similar to mouse chromosomes. The X chromosome and 3) Identify mouse chromosomes which contain sections that are not homologous to human chromosomes. 4) Suggest reasons for the many similarities between the mouse and human genomes. 5) Deduce how chromsomes have mutated during the evolution of animals such as mice and humans. March 6, 2013 – DBQ on p. 168 1) State the most common number of differences in base sequence between pairs of humans. 8 differences in base sequences 2) Humans and Neanderthals are both classified in the genus Homo and chimpanzees are classified in the genus Pan. Discuss whether this classification is supported by the data in the bar chart. This classification is supported by the data in the bar chart because the most common number of differences in base sequences between humans and Neanderthals, 27, is a lot smaller than the number of differences in base sequences between humans and chimps, 56. This suggests that humans are more similar to Neaderthals than chimps, thus, it makes sense that humand and Neanderthals are both classified in the same genus. Furthermore, the number of differences in base sequences between humans and Neanderthals and the number of differences in base sequences between pairs of humans are very close, suggesting that the two species are still very similar. 3) Suggest a limitation to drawing any conclusion from the human-Neanderthal comparison. A limitation to drawing any conclusion from the human-Neanderthal comparison is that the DNA used was only from one Neanderthal, which means that there isn’t enough basis to effectively support any conclusions drawn.